Plant Variety Journal - IP Australia

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Plant Varieties Journal Vol. 23 Number 4 Details of Application Application Number 2008/275 Variety Name Riverina Thomas Genus Species Lavandula x intermedia Common Name Lavandin Synonym Nil Accepted Date 15 Dec 2008 Applicant Charles Sturt University Agent N/A Qualified Person Nigel Urwin, Charles Sturt University, Wagga Wagga, NSW. Details of Comparative Trial Location Charles Sturt University Descriptor Lavandula (Lavandula) TG/194/1 Period Sep 2009-Dec 2010 Conditions All plants were propagated by Larkman Nurseries, Lilydale, Melbourne and provided in 50x75mm tubes. All plants were potted into 9cm diameter pots in Debco TM Terracotta and Tub potting mix. Plants were watered every other day by hand. Plants were repotted into 25cm diameter pots in the same growing medium late Aug 2010. Plants were then watered by automatic overhead sprinklers and occasionally by hand. Osmocote TM slow release fertiliser and fungicides were applied occasionally as required. Trial Design The trial consisted of 8 L.x intermedia ‘Hidcote Giant’, 10 L.x intermedia ‘Impress Purple’, 10 L.x intermedia ‘Seal’, 9 L.x intermedia ‘Grosso’ 10 L. hybrid ‘Riverina Thomas’ and 10 L. hybrid ‘Riverina Alan’. Plants were arranged in a completely randomised block design (10x6). The trial was designed for DUS analysis of two varieties the other being Riverina Alan which was included as a comparator also Measurements Observations were made on all varieties, mid- flowering on 13th Dec 2010 and also early Oct before flowering for observation on plant size and form. RHS Chart - edition Fifth edition Origin and Breeding Open pollination: The starting material was L.x intermedia ‘Grosso’. This is the most commonly grown variety of L.x intermedia world-wide. Shoots of glasshouse grown ‘Grosso’ plants were excised and stood in 0.1% colchicine for 16 hr to induce polyploidy. The shoots were rinsed with water and propagated by dipping in rooting hormone and planting in seed raising mix. Plants were maintained on misting beds at 25°C until either roots formed or the cuttings died. Surviving plants with roots were potted and grown to flowering in the lavender collection at Charles Sturt University, Wagga Wagga. The plants were allowed to flower under conditions which allowed open pollination. All known L.x intermedia varieties are infertile hybrids of L. angustifolia and L. latifolia. They occur naturally in the wild and some have been bred. Consequently they do not produce seed. Often conversion of a diploid sterile hybrid to tetraploid results in restoration of fertility and seed production in other genera. It was anticipated that similar conversion of L.x intermedia ‘Grosso’ to tetraploidy status would restore fertility. To detect tetraploids we therefore attempted to collect seed from the ‘Grosso’ plants surviving colchicine treatments. A number of plants which had colchicine treatments Page 152 of 322
Plant Varieties Journal Vol. 23 Number 4 produced seed whereas none of the controls plants treated with water rather than colchicine produced any seed. Ten seeds obtained from a single colchicine treated ‘Grosso’ cutting were germinated in a petri-dish on filter paper soaked in gibberellic acid to induce germination. All the seeds germinated and plants were potted up and grown until flowering. The plants were grown under the same conditions as the parent plants above. Cuttings from individual plants were propagated and planted in the field collection to observe their morphology and performance. Of the ten plants grown some morphological variation was seen however most were more vigorous and much larger plants than the parent variety ‘Grosso’. The plant with the greatest vigour and largest flowers was selected. This plant was designated ‘Riverina Thomas’ or CSU150. All plants called ‘Riverina Thomas’ were clonally propagated as cuttings from the one plant. Characteristics of L.x intermedia ‘Riverina Thomas’ are that it is a larger plant in the field than ‘Grosso’ with larger leaves and flowers. It has thicker peduncles and stems, is large but compact and globular in form and flowers slightly earlier than ‘Grosso’. From chromosome number estimates and flow cytometry analysis of nuclear DNA content ‘Riverina Thomas’ and the other ‘Grosso’ derived seedlings are approximately triploid whereas the parent ‘Grosso’ was diploid. ‘Riverina Thomas’ is infertile and did not produce any seed tested over two seasons in the collection at CSU. In summary, the diploid L.x intermedia ‘Grosso’ (infertile) was converted to a tetraploid (fertile). Following open pollination of the tetraploid seeds were collected from these and ‘Riverina Thomas’ is a seedling selected on the basis of size of the whole plant and various plant organs. Choice of Comparators Characteristics used for grouping varieties to identify the most similar Variety of Common Knowledge Organ/Plant Part Context State of Expression in Group of Varieties Plant size medium Most Similar Varieties of Common Knowledge identified (VCK) Name Comments ‘Grosso’ Parent. ‘Hidcote Giant’ ‘Impress Purple’ ‘Seal’ ‘Riverina Alan’ Variety Description and Distinctness - Characteristics which distinguish the candidate from one or mo of the comparators are marked with a tick. Organ/Plant Part: ‘Riverina ‘Hidcote ‘Impress ‘Riverina ‘Grosso’ ‘Seal’ Context Thomas’ Giant’ Purple’ Alan’ *Plant: growth habit globular globular globular globular bushy bushy *Plant: size medium medium medium medium medium medium Plant: intensity of green colour of foliage Plant: intensity of grey tinge of foliage *Plant: attitude of outer flowering stems medium medium medium medium medium medium medium to strong medium to strong medium to strong medium to strong medium to strong semi-erect semi-erect erect semi-erect erect erect medium to strong *Plant: density medium medium medium medium medium medium Page 153 of 322
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