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44. Volume 15- Number 2 - IP Australia

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DESCR<strong>IP</strong>TIONS<br />

PLANT VARIETIES JOURNAL 2002 VOL <strong>15</strong> NO. 2<br />

TIME OF MATURITY<br />

early to medium late<br />

medium to early<br />

____________________________________________________<br />

Trifolium pratense<br />

Red Clover<br />

‘Crossway’<br />

Application No: 2002/091 Accepted: 27 May 2002.<br />

Applicant: AgResearch Limited, Palmerston North, New<br />

Zealand.<br />

Agent: Denis McGrath, Drumcondra, VIC.<br />

Characteristics (Table 43, Figure 58) Ploidy: diploid.<br />

Plant: growth habit prostrate, natural height in spring short,<br />

width broad, maturity medium. Stem: density very high,<br />

length long (59.4cm), thickness thin (2.80mm), intensity of<br />

anthocyanin colouration medium, density of hairs medium,<br />

internode length (at 4 th internode) medium to long<br />

(11.7cm), number of internodes per stem medium to high<br />

(mean 12.1). Leaf: shape of medial leaflet ovate, length of<br />

medial leaflet medium to short (22.9mm), width of medial<br />

leaflet narrow (8.9mm), frequency of plants with white<br />

marks very high (98%), intensity of green colour in spring<br />

light. Time of flowering: medium (41.3 days from 1 st Nov).<br />

Flower: colour medium purple 45% RHS 77C-D, dark<br />

purple 54% RHS 77B. Seed: colour of coat multicoloured,<br />

distribution of colour approximately even.<br />

Origin and Breeding Phenotypic selection followed by<br />

polycross: ‘Crossway’ originated from selections within 27<br />

naturalised populations in Spain and Portugal collected in<br />

1986 by consensual arrangement with relevant authorities.<br />

Several plants from each population were grown at<br />

Palmerston North, New Zealand from 1987-9; and about 60<br />

were then removed and inter-pollinated in isolation. The<br />

parent plants were characterised by semi-prostrate growth<br />

habit, variable formonenetin levels, variable stem thickness,<br />

sparse to dense stem density and variable flowering. The<br />

seed harvested was bulked, grown and further selections<br />

made for prostrate and creeping habit in grazed swards for<br />

two generations. In 1995, about 250 plants were potted and<br />

measured for formononetin levels in leaves and scored for<br />

habit. Those with lowest levels of formononetin and<br />

prostrate habit were inter-pollinated and further selections<br />

continued each year for these characteristics until 1998. The<br />

selected plants were inter-pollinated and a further selection<br />

made for high seed yield. The final generation of selection<br />

for habit and low formononetin was harvested in 1999 as<br />

nucleus seed and code named GF67 and later named<br />

‘Crossway’. Selection criteria: growth habit, formononetin<br />

levels in leaves and potential seed yield. Propagation: seed.<br />

Breeder: Dr W. (Bill) Rumball, AgResearch Grasslands,<br />

Palmerston North, New Zealand.<br />

Choice of Comparators The grouping characteristic used<br />

in identifying the most similar varieties of common<br />

knowledge was – Ploidy: diploid. On this basis,<br />

‘Broadway’, ‘Sensation’ ‘Grasslands Hamua’. ‘Grasslands<br />

Colenso’, ‘Grasslands Turoa’, ‘Astred’. ‘Redwest’,<br />

‘Redquin’, ‘Quinequeli’, ‘Renegade’ and ‘PAC 19’ were<br />

included in the PBR trial as comparators. The parental<br />

plants were not included because ‘Crossway’ differed from<br />

the parents in having a more prostrate growth habit and<br />

lower levels of formonenetin in leaf levels (Dr W. (Bill)<br />

Rumball pers.comm.). ‘Grasslands G27’ was not included<br />

as it is a tetraploid variety.<br />

Comparative Trial Comparators: Location: AgResearch<br />

Grasslands Research Centre, Palmerston North, New<br />

Zealand (Latitude 40º23′ South, elevation 33m), autumnsummer<br />

2000-2002. Conditions: plants raised from seed<br />

sown on 22/3/00 (trial 1) and <strong>15</strong>/3/01 (trial 2) in seed flats<br />

in controlled glasshouse conditions. Plants trimmed on<br />

28/4/00 (trial 1) and <strong>15</strong>/3/01 (trial 2) to enhance<br />

establishment and placed in the open for hardening. Plants<br />

transplanted into open field site on 8/7/00 (trial 1) and 8-<br />

11/6/01 (trial 2) at 60cm between plants and 120cm<br />

between plots. Trial design: randomised block of 10 plots of<br />

10 plants of each variety arranged in a completely<br />

randomised design in each block. Measurements: from all<br />

available plants.<br />

Prior Applications and Sales<br />

Country Year Current Status Name Applied<br />

New Zealand 2001 Applied ‘Crossway’<br />

Prior sale nil.<br />

Description: Jeff E. Miller, AgResearch Grasslands, Palmerston North,<br />

NZ.<br />

Table 43 Trifolium varieties<br />

‘Crossway’ *‘Broadway’ *‘Sensation’ *‘G. Hamua’ *‘G. Colenso’*‘G. Turoa’ *‘Astred’ *‘Redwest’ *‘Redquin’ *‘Quinqueli’ *‘Renegade’ *‘PAC19’<br />

______________________________________________________________________________________________________________<br />

DAYS TO MEAN FLOWERING (Days from 1st flower on 1/11/2000)<br />

mean 41.3 42.6 42.0 46.2 46.0 74.5 42.7 34.5 49.0 53.6 34.7 61.9<br />

std deviation 8.67 9.45 12.5 16.2 13.8 7.0 12.7 12.2 7.4 5.8 <strong>15</strong>.1 14.8<br />

LSD/sig 4.9 ns ns P≤0.01 ns P≤0.01 ns P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01<br />

______________________________________________________________________________________________________________<br />

STEM LENGTH (cm)<br />

mean 59.4 64.7 63.2 66.6 60.7 79.0 80.7 56.6 72.5 95.5 64.5 75.5<br />

std deviation 12.2 11.9 13.5 18.0 <strong>15</strong>.6 10.0 18.5 <strong>15</strong>.2 13.6 17.3 16.2 16.9<br />

LSD/sig 6.5 ns ns P≤0.01 ns P≤0.01 P≤0.01 ns P≤0.01 P≤0.01 ns P≤0.01<br />

______________________________________________________________________________________________________________<br />

STEM THICKNESS (mm)<br />

mean 2.80 3.<strong>15</strong> 3.82 3.79 3.45 3.75 3.57 3.76 3.87 3.84 4.30 4.51<br />

std deviation 0.38 0.44 0.56 0.60 0.40 0.45 0.49 0.62 0.57 0.45 0.69 0.70<br />

LSD/sig 0.27 P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01 P≤0.01<br />

______________________________________________________________________________________________________________<br />

79

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