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A B S T R A C T
RGUHS Journal of Pharmaceutical Sciences
Influence of Vitamin C with Lansoprazole in Pylorus Ligation Induced Ulcer
Model in Rats
Nitin M*, Prasad K, Girish M, Ather Javed, Chetan M and Krunal S
Department of Pharmacology, HKES College of Pharmacy, Sedam Road, Gulbarga-585105, Karnataka, India.
Gastric hyperacidity and ulceration of stomach mucosa due to various factors are serious health problems of global concern. Though
currently available drugs provide adequate relief against ulcer, the problem of relapse has eluded success. Hence an attempt is being
made to find a therapy with more efficacy and better results. The present study was designed to evaluate the combination effect of
vitamin C with lansoprazole against pylorus ligation induced ulcer model in rats. The antiulcer effect of the combination of vitamin C 4.5
mg/200 g and lansoprazole 0.54 mg/200 g b.w orally was compared with the reference standard lansoprazole 0.54 mg/200 g b.w orally.
The ulcer index was calculated and other biochemical parameters of gastric juice were estimated. The ulcer index of combination
showed significant (P < 0.05) reduction while other biochemical parameters like volume, pH, free acidity and total acidity of gastric juice
showed highly significant (P < 0.001) reduction when compared to control and standard lansoprazole. The percentage protection of
combination was 94.3% as compared to standard lansoprazole 82.8%. Thus the combination group was found to be synergistic in nature
when compared to lansoprazole alone.
Keywords: Antiulcer activity, vitamin C, lansoprazole, pylorus ligation, ulcer index.
INTRODUCTION
Gastric ulcer, the most common disorder of GIT has
1
multifunctional causes in its pathophysiology . The
pathophysiology of peptic ulcer has been centralized on an
imbalance between aggressive and protective factors in the
stomach such as acid-pepsin secretion, mucosal barrier,
mucus secretion, blood flow, cellular regeneration,
prostaglandins and epidermal growth factors. Various causes
of gastric ulceration include stress, alcohol, Helicobacter pylori
and use of NSAIDs have been shown to be mediated largely
through generation of reactive oxygen species (ROS),
2
especially the hydroxyl radical . A number of excellent drugs,
developed over the decades have proven useful in controlling
hyperacidity and ulceration but their long term use is reported
to have various side effects. Hence the investigation with an
objective to find a therapy with more efficacy and lesser side
effects.
Vitamin-C is a well known antioxidant that neutralizes
various reactive oxygen species such as superoxide radical,
3
singlet oxygen, hydrogen peroxide , alkoxyl, hydroxyl radical
3
directly by hydrogen donation and also neutralizes the radical
4
form of other antioxidants like glutathione & vitamin-E .
Apart from scavenging free radicals it translates haemeoxgenase-1
mRNA into active protein, which then may exert
gastroprotection by its antioxidant and vasodilative
RGUHS Journal of Pharmaceutical Sciences
Received: 12/3/2011, Modified: 28/7/2011, Accepted: 20/8/2011
127
Original Research Article
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properties . Hence an attempt was made to study the
combination effect of vitamin C with antisecretory drugs like
lansoprazole. Lansoprazole is a proton pump inhibitor that
+ +
suppresses the gastric acid secretion by inhibiting H K
ATPase pump. The FDA has approved it for treatment and
prevention of recurrence of NSAIDs associated gastric ulcers
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in patients who continue NSAID use . It is more potent, has
longer duration of action, better bioavailability and lesser
drug interaction than other drugs. Therefore, the present
study is designed to evaluate the combination of vitamin C
and lansoprazole against pylorus ligation induced ulcer
model.
MATERIALS AND METHODS
Albino wistar rats of either sex weighing between 180 to 220 g
were selected for the present study. The animals were
acclimatized for seven days and housed under standard
conditions of temperature (25±2°C) and relative humidity
(30-70%) with a 12:12 light-dark cycle. The animals were fed
with standard pellet diet (Hindustan liver co. Mumbai) and
water ad libitum. Pure drug samples of lansoprazole and
vitamin C were procured from Lee Pharmaceuticals
(Hyderabad.) and Wockhardt Ltd (Aurangabad,
Maharashtra.). The dose calculations were extension of
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human dose based on body surface area . Animal studies were
performed with prior permission of Institutional Animal
Ethics Committee (IAEC) of H.K.E.S College of pharmacy,
Gulbarga (Protocol No. HKECOP/ IAEC/ 16/ 2009-10/
CPCSEA).
RJPS, Jul - Sep, 2011/ Vol 1/ Issue 2