Annual Report 2006

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An evolutionay sequence was suggested most likely from type I to type IV, from type IV to type IX, and from type IX to type III (Fig. 3). Types IVa and IVb were independently formed from type IV by different short insertions. Type VI was also evolved from type I, and type VIII from type VI. Five landraces of type V ( ) and one of type X () have an completely identical LTR sequence. The LTRs of were identical among six landraces of type VII. These findings indicate that the insertions occurred quite recently from a paleontological viewpoint. Allele types I to X can be identified by Long PCR using four sets of primers, and additional two sets can distinguish types IVa and IVb from type IV. Publication 1) Kawase M., K. Fukunaga, K. Kato (2005) Diverse origins of waxy foxtail millet crops in East and Southeast Asia mediated by multiple transposable element insertions. Mol. Gen. Genomics, 274:131-140. Development of practical method to discriminate Nagoya breed from other chicken breeds Hideaki TAKAHASHI, Akihiro NAKAMURA* and Mitsuru MINESAWA Genebank *Akichi-ken Agricultural Research Center Nagoya breed (Fig. 1) is a famous native chicken in Aichi Prefecture of Japan, a dual purpose breed for eggs and meat. The purebred (Nagoya breed Nagoya breed) has been commercialized, so the commercial chickens are produced by intra-strain mating. Nagoya breed has four strains (NG1, NG2, NG3 and NG4) established at Aichi-ken Agricultural Research Center. They are maintained at Aichi Fig. 1 Nagoya breed Livestock and Poultry Breeding Center, which supplies parent stocks to the hatcheries using the four strains. Therefore, all commercial Nagoya breed chicken are derived from four strains. Since the taste, palatability and texture of Nagoya breed meat are well recognized in Japan, the market price of Nagoya breed meat is much higher than that of broiler meat. However, cut-up meat of Nagoya breed and broiler cannot be easily distinguished by appearance. So, a technology to discriminate between Nagoya breed and broiler is vital for preventing false sales and guaranteeing the quality of meat. The objective of the present study is to develop a method to discriminate between Nagoya breed and all other chicken on the market using microsatellite DNA markers. Four strains of Nagoya breed established at the Aichi-ken Agricultural Research Center were analyzed using twenty-five microsatellite markers. In these strains, five of the markers
(ABR0015, ABR0257, ABR0417, ABR0495 and ADL0262) had a single allele. Other 448 chicken samples of various breeds and hybrids were analyzed using the same five markers. None of these chicken samples had the same allele combination as the Nagoya breed. These five microsatellite markers provide a practical method to accurately discriminate the Nagoya breed from other chicken and can contribute to checking the validity of labeling Nagoya breed. Intrabody technology: a novel method for domain-specific knockdown of cytosolic protein in mice Mitsuru SATO Molecular Biology and Immunology Department Intracellularly expressed antibodies (intrabodies) have been considered useful for not only clinical applications such as viral neutralization and cancer therapy but also functional analysis of proteins inside the cell. A variety of intrabody formats have been designed. Single-chain variable fragments (scFvs) consist of one heavy chain variable region (VH) linked through a flexible peptide spacer, a repeated motif of 3 GGGGS, to one light chain variable (VL). They are able to fold and retain the antigen-binding specificity and affinity of the parental antibody. However, technical problems, such as stability and functional expression of intrabodies in the cytosol remain to be overcome. In this study, we constructed the scFv intrabodies derived from hybridoma cells producing the monoclonal antibodies (Fig. 1A) that specifically bind to an Ena/VASP homology 1 (EVH1) domain of Wiskott-Aldrich syndrome protein (WASP). WASP resides in the cytosol as a multifunctional adaptor molecule and mediates actin polymerization Fig. 1 Antibody structure (A) The basic structure of a conventional antibody consists of four polypeptide, two identical heavy (H) chains and two identical light (L) chains, held together by disulfide bonds. Each chain has both a variable (V) and a constant (C) region. (B) Schematic representation of the two scFv DNA constructions for microinjection. Shown are the leader signal sequence, VH region, polypeptide linker (Gly4Ser)3, VL region, light chain constant (CL) region and Myc tag sequence. The scFv DNA fragments were cloned into the pCAGGS-MCS expression vector.
Page 1 and 2: 2006
Page 3 and 4: Teruo Ishige, President The Nationa
Page 5 and 6: List of Publication Original paper
Page 7 and 8: Topics of Research in This Year Pos
Page 9 and 10: studies. To construct a fully satur
Page 11: Fig. 2 Geographical distribution of
Page 15 and 16: Mulberry latex defense mulberry tre
Page 17 and 18: Fig. 2 Growth inhibitory effects of
Page 19 and 20: Fig. 3 Change of the levels of luci
Page 21 and 22: Next, we examined the effect of tra
Page 23 and 24: G enome Research Department Introdu
Page 25 and 26: The Rice Annotation Project Databas
Page 27 and 28: industrial and agricultural concern
Page 29 and 30: One of the main problems involving
Page 31 and 32: Fig. 3 Mapping of QTL for rachis in
Page 33 and 34: two genes, XOO2263 and XOO4134 cont
Page 35 and 36: G enebank Genebank Project The NIAS
Page 37 and 38: using dormant winter buds. Also, we
Page 39 and 40: Insect and Animal Sciences Division
Page 41 and 42: Expression patterns of two novel ge
Page 43 and 44: Fig. 5 PGC and EBC fused cells PGCs
Page 45 and 46: cells. The epidermal cells maintain
Page 47 and 48: Animal and cell culture models for
Page 49 and 50: terminus, respectively. A signal se
Page 51 and 52: Fig. 2 Trehalose content of body pa
Page 53 and 54: Fig. 6 Birth weight of 11 adult SNT
Page 55 and 56: The role of glucose as a metabolic
Page 57 and 58: analysis using microsatellite marke
Page 59 and 60: Fig. 2 Recorded Electromyograms (le
Page 61 and 62: Table 1 Menu of Commercial silkworm
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Fig. 3 Expression of GFP gene in th
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also surveyed in the genetic stocks
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esearch were located in or near gen
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elatives results in severe reductio
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and circadian clocks may control th
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B iochemistry Department Structural
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Proteomic analysis Based on the pro
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photoautotrophic medium (Fig. 1B).
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Fig. 4 Blast resistance of OsWRKY45
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phosphatase activity by SIPK depend
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the endosperm in co-operation with
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isolated genes in transgenic Anal
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Fig. 1 Gene structures and mutation
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cytochimera among the mulberries (
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15 G. Hariraj, Kinoshita Haruo, T.
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49 Kameda Tsunenori, Teramoto Hidet
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85 Matsuyama Shuichi, Ohkura Satosh
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120 Ozawa Kenjiro, Kawahigashi Hiro
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155 Taguchi-Shiobara Fumio, Yamamot
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192 Ueno Osamu, Agarie Sakae, (2005
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8 Murakami Ritsuko, Koyama Akio, Sh
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20 Todoroki Junichi, Kaneko Hiroyuk
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Executive Members and Research Staf
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Developmental Biology Department Di
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Research Leader Hayasaka Shoji Rese
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Mutation Genetics Laboratory Head N
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thousands of yen TOTAL BUDGET OPERA
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Annual Report 2006 (Apr. 2005Mar. 2
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Annual Report 2006

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