Annual Report 2006

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President Vice President(Insect and Animal Sciences) Vice President(Plant Sciences) Auditor Department of Research Planning and Coordination Research Planning Division Research Evaluation Division Research Coordination Section Office for GMO Research and Development Technology Transfer Section Administration Section Information and Public Relation Section Field Management Section Genome Resource Center QTL Genomics Research Center Center for GMO Research and Information Insect Gene Function Research Center Department of Administration General Affairs Section Accounting Section Facility Management Section Director of Genome and Biodiversity Research Division Genome Research Department Plant Genome Laboratory Animal Genome Laboratory Insect Genome Laboratory Bioinformatics Laboratry DNA Bank Genetic Diversity Department Molecular Biodiversity Laboratory Biosystematics Laboratory Evolutionary Dynamics Laboratory Germ Cell Conservation Laboratory Applied Microbiology Laboratory Adaptation Systems Laboratory Biometrics Laboratory Genebank Plant Genetic Resources Labolatory Microorganism Genetic Resources Laboratory Animal Genetic Resources Laboratory Genetic Resources Management Section Developmental Biology Department Developmental Mechanisms Laboratory Developmental and Differentiation Laboratory Animal Genetic Engineering Laboratory Embryonic Technology Laboratory Insect Growth Regulation Laboratory Reproductive Biology and Technology Laboratory Molecular Biology and Immunolory Laboratory Molecular Immunolory Laboratory Innate Immunity Laboratory Experimental Animals Laboratory Physiology and Genetic Regulation Department Insect Life-Cycles and Physiology Laboratory Insect Nutrition and Matabolism Laboratory Insect Neurobiology Laboratory Insect Behavior Laboratory Animal Gene Function Laboratory Animal Cell Biology Laboratory Animal Neurophysiolory Laboratory Animal Neuroendocrinnolory Laboratory Insect Genetics and Evolution Department Insect-Plant Interactions Laboratory Natural Enemies Laboratory Symbiosis Laboratory Insect Patholory Laboratory Insect Genetics Laboratory Insect Molecular Evolution Laboratory Insect Biomaterial and Technology Department Biopolymer Characterization Laboratory Biomaterial Development Laboratory Biomimetic Laboratory Insect Products Utilization Laboratory Insect Biotechnology and Sericology Department Insect Cell Engineering Laboratory Insect Gene Engineering Laboratory Mass Production System Laboratory New Silk Materials Laboratory Sericultural Science Laboratory Molecular Genetics Department Functional Genomics Laboratory Applied Genomics Laboratory Epigenetics Laboratory Gene Expression Laboratory Gene Regulation Laboratory Biochemistry Department Crystallography Laboratory Biophysics Laboratory Glycobiology Laboratory Membrane Biology Laboratory Plant Physiology Department Photosynthesis Laboratory Carbon Metabolism Laboratory Development Biology Laboratory Environmental Physiology Laboratory Disease Physiology Laboratory Nitrogen Fixation Laboratory Plant Biotechnology Department Gene Design Laboratory Plant Gene Engineering Laboratory Plant Cell Engineering Laboratory Molecular Breeding Laboratory Biosystems Laboratory Institute of Radiation Breeding Mutation Genetics Laboratory Radiation Technology Laboratory Mutation Breeding Laboratory
Topics of Research in This Year Positional cloning of a densovirus-resistance gene in Keiko KADONO-OKUDA, Katsuhiko ITO, Junko NOHATA, Kimiko YAMAMOTO, Motoe SASANUMA, Shunichi SASANUMA, Ryokitsu EGUCHI, Wajiro HARA and Kazuei MITA Genome Research Department densovirus ( BmDNV ) multiplies in the columnar cell nuclei of the midgut epithelia of the silkworm, . It is classified into two species, DNV-1 and DNV -2 based on their symptoms, serological characters, genome structure and sequences. Some silkworm strains were identified as resistant against DNV-1 and/or DNV-2. In such strains the response reflects non-susceptibility rather than resistance because even high dose of inoculum does not affect their survival rate. So far four non-susceptibility genes have been reported, namely; (L21-8.3 cM), (L17 -31.1 cM), (L17-24.5 cM) and (L15- 50.7 and 30.0 cM from apical and distal ends, respectively). However, none of them have yet been isolated as responsible genes. Studies on these genes are useful in understanding the mechanism of the viral invasion and multiplication, and introducing those genes into the silkworms or cultured cells will lead us to understand the functions of the genes. We have identified four EST markers closely linked with By taking advantage of Bombyx genome information, positional cloning, BAC-contig construction and linkage analysis of the virusselected BC1 (backcross 1) progenies (Fig. 1) enabled to find the resistant strain-specific deletion within the candidate region of 500 kb (Fig. 2). We have succeeded in identifying the candidate gene for on the deletion region (Fig. 3). The gene was expressed only in the midgut throughout the larval stage. RT-PCR revealed that mutant strains examined showed a common shorter transcript compared with that of susceptible strains, which was caused by the common deletion in the gene among resistant strains (Fig. 4). Sequence analysis of the cDNA tells that encodes a transmembrane protein (Fig. 5). Mutation of this gene endows the resistance against the virus. Identification of gene strongly accelerates the virus research through establishing transgenic cultured cell lines hypersensitive to the virus, in addition to the direct marker-assisted breeding of strains resistant to DNV-2. Fig. 1 Segregation of BC1 with DNV-2 infection.
Page 1 and 2: 2006
Page 3 and 4: Teruo Ishige, President The Nationa
Page 5: List of Publication Original paper
Page 9 and 10: studies. To construct a fully satur
Page 11 and 12: Fig. 2 Geographical distribution of
Page 13 and 14: (ABR0015, ABR0257, ABR0417, ABR0495
Page 15 and 16: Mulberry latex defense mulberry tre
Page 17 and 18: Fig. 2 Growth inhibitory effects of
Page 19 and 20: Fig. 3 Change of the levels of luci
Page 21 and 22: Next, we examined the effect of tra
Page 23 and 24: G enome Research Department Introdu
Page 25 and 26: The Rice Annotation Project Databas
Page 27 and 28: industrial and agricultural concern
Page 29 and 30: One of the main problems involving
Page 31 and 32: Fig. 3 Mapping of QTL for rachis in
Page 33 and 34: two genes, XOO2263 and XOO4134 cont
Page 35 and 36: G enebank Genebank Project The NIAS
Page 37 and 38: using dormant winter buds. Also, we
Page 39 and 40: Insect and Animal Sciences Division
Page 41 and 42: Expression patterns of two novel ge
Page 43 and 44: Fig. 5 PGC and EBC fused cells PGCs
Page 45 and 46: cells. The epidermal cells maintain
Page 47 and 48: Animal and cell culture models for
Page 49 and 50: terminus, respectively. A signal se
Page 51 and 52: Fig. 2 Trehalose content of body pa
Page 53 and 54: Fig. 6 Birth weight of 11 adult SNT
Page 55 and 56: The role of glucose as a metabolic
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analysis using microsatellite marke
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Fig. 2 Recorded Electromyograms (le
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Table 1 Menu of Commercial silkworm
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Fig. 3 Expression of GFP gene in th
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also surveyed in the genetic stocks
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esearch were located in or near gen
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elatives results in severe reductio
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and circadian clocks may control th
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B iochemistry Department Structural
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Proteomic analysis Based on the pro
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photoautotrophic medium (Fig. 1B).
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Fig. 4 Blast resistance of OsWRKY45
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phosphatase activity by SIPK depend
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the endosperm in co-operation with
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isolated genes in transgenic Anal
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Fig. 1 Gene structures and mutation
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cytochimera among the mulberries (
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15 G. Hariraj, Kinoshita Haruo, T.
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49 Kameda Tsunenori, Teramoto Hidet
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85 Matsuyama Shuichi, Ohkura Satosh
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120 Ozawa Kenjiro, Kawahigashi Hiro
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155 Taguchi-Shiobara Fumio, Yamamot
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192 Ueno Osamu, Agarie Sakae, (2005
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8 Murakami Ritsuko, Koyama Akio, Sh
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20 Todoroki Junichi, Kaneko Hiroyuk
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Executive Members and Research Staf
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Developmental Biology Department Di
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Research Leader Hayasaka Shoji Rese
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Mutation Genetics Laboratory Head N
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thousands of yen TOTAL BUDGET OPERA
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Annual Report 2006 (Apr. 2005Mar. 2
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Annual Report 2006

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