Annual Report 2006

More documents

Recommendations

Info

Fig. 4 The ventral surfaces of female 5th foretarsal segment of 1: 2: 3: 4: 5: , 6: 7: 8: 9: 0: .D: P: Fig. 5 Amale landing on an untreated black lure (-) paired with a white lure (+) treated with pheromone (10 mg of anthranilic acid) and set on a lawn with a stick and bar The interval between lures is 5 cm. Visual pinpoint location associated with pheromonal cue in males of the black chafer (Coleoptera: Scarabaeidae) Females of the black chafer (Sawada) (Coleoptera: Scarabaeidae) release anthranilic acid, which functions as sex attractant pheromone for males and aggregation pheromone for females. When a white lure treated with anthranilic acid was placed next to an untreated black lure in the field, males were observed to make pinpoint landings significantly more frequently on the latter (Fig. 5). When the distance between the two lures was increased from 0 cm to 20 cm, frequency of pinpoint landing onto the untreated black lures significantly decreased while that onto the treated white lures slightly increased. When the lures were further separated to 2-m intervals, males approached only to the treated lures regardless of the color but significantly more frequently landed on the black ones than on the white. These observations demonstrated that males locate and land on a female by visual cues after reaching the vicinity by olfaction. Relationship between imprinting disorder and overweight at birth in bovine somatic cell nuclear transfer clones Genomic imprinting, which is the parentalorigin-specific gene regulation mechanism in mammals, plays essential roles in development and growth. The mechanisms discriminating between paternal and maternal genes are not clear yet but DNA methylation as epigenetic marker is thought to be a major clue for their recognition. DNA methylation for parental imprinted markers persists in somatic cells after fertilization, and is erased and reestablished in germ cells according to sex. Recent remarkable technique of somatic cell nuclear transfer (SNT) cloning made us to reproduce animals without germ cell transmission and fertilization. But survival rate of SNT clone at birth is still miserable. One possibility of this inefficiency has been thought to be epigenetic disorder. Weight of bovine SNT clones at birth are usually heavier than average and the neonatal survival rate is low. We had determined bovine imprinting genes and identified several genes expressed paternally monoallelic in Holstein Japanese black families. Based on the information, we successively investigated allelic expressions for polymorphic imprinting genes in adult
Fig. 6 Birth weight of 11 adult SNT clones Red characters indicate individuals with bi-allelic expression of imprinting gene(s). Blue characters indicate those with mono-allelic ones. Green circle and yellow square indicate average birth weight of female and male artificially inseminated(AI). Japanese black SNT clones. Surprisingly, almost half of healthy and well-grown adult bovine SNT clones had imprinting disorder, i.e. bi-allelic expressions. Some of them were also confirmed fertile. These results were clearly indicated that imprinting disorder was common for bovine SNT clones with no apparent abnormality in adulthood. But our further analysis revealed the relationship between overweight at birth and bi-allelic disorder (Fig. 6). Our results indicate that the management of imprinting will crucial for effective SNT clone reproduction. Protein-permeable scaffold of a collagen vitrigel membrane useful for reconstructing crosstalk models between two different cells We recently succeeded in converting a soft andturbiddiskofcollagengelintoastrongand transparent gel-membrane by utilizing a concept for the vitrification of heat-denatured proteins and named the novel gel in a stable condition for vitrigel. The collagen vitrigel membrane involving a nylon frame can be easily handled with tweezers, consequently it functions as a scaffold excellent for three-dimensionally culturing cells on double surfaces of it. Here, we investigated the molecular permeability of the collagen vitrigel membrane in time-course using glucose and serum proteins. The glucose added to one compartment penetrated into another compartment via the collagen vitrigel membrane and the glucose concentration of each compartment came nearly up to a plateau level within 24 hours. Serum proteins with not only low molecular weight but also high one more than 100 kDa passed gradually through the collagen vitrigel membrane (Fig. 7). These Fig. 7 Permeability tests in time-course for the collagen vitrigel membrane using glucose and serum proteins Change of glucose concentration (A), quantitative clearance of FBS (B), and qualitative clearance of FBS (C) were estimated using the samples at the indicated times.
Page 1 and 2: 2006
Page 3 and 4: Teruo Ishige, President The Nationa
Page 5 and 6: List of Publication Original paper
Page 7 and 8: Topics of Research in This Year Pos
Page 9 and 10: studies. To construct a fully satur
Page 11 and 12: Fig. 2 Geographical distribution of
Page 13 and 14: (ABR0015, ABR0257, ABR0417, ABR0495
Page 15 and 16: Mulberry latex defense mulberry tre
Page 17 and 18: Fig. 2 Growth inhibitory effects of
Page 19 and 20: Fig. 3 Change of the levels of luci
Page 21 and 22: Next, we examined the effect of tra
Page 23 and 24: G enome Research Department Introdu
Page 25 and 26: The Rice Annotation Project Databas
Page 27 and 28: industrial and agricultural concern
Page 29 and 30: One of the main problems involving
Page 31 and 32: Fig. 3 Mapping of QTL for rachis in
Page 33 and 34: two genes, XOO2263 and XOO4134 cont
Page 35 and 36: G enebank Genebank Project The NIAS
Page 37 and 38: using dormant winter buds. Also, we
Page 39 and 40: Insect and Animal Sciences Division
Page 41 and 42: Expression patterns of two novel ge
Page 43 and 44: Fig. 5 PGC and EBC fused cells PGCs
Page 45 and 46: cells. The epidermal cells maintain
Page 47 and 48: Animal and cell culture models for
Page 49 and 50: terminus, respectively. A signal se
Page 51: Fig. 2 Trehalose content of body pa
Page 55 and 56: The role of glucose as a metabolic
Page 57 and 58: analysis using microsatellite marke
Page 59 and 60: Fig. 2 Recorded Electromyograms (le
Page 61 and 62: Table 1 Menu of Commercial silkworm
Page 63 and 64: Fig. 3 Expression of GFP gene in th
Page 65 and 66: also surveyed in the genetic stocks
Page 67 and 68: esearch were located in or near gen
Page 69 and 70: elatives results in severe reductio
Page 71 and 72: and circadian clocks may control th
Page 73 and 74: B iochemistry Department Structural
Page 75 and 76: Proteomic analysis Based on the pro
Page 77 and 78: photoautotrophic medium (Fig. 1B).
Page 79 and 80: Fig. 4 Blast resistance of OsWRKY45
Page 81 and 82: phosphatase activity by SIPK depend
Page 83 and 84: the endosperm in co-operation with
Page 85 and 86: isolated genes in transgenic Anal
Page 87 and 88: Fig. 1 Gene structures and mutation
Page 89 and 90: cytochimera among the mulberries (
Page 91 and 92: 15 G. Hariraj, Kinoshita Haruo, T.
Page 93 and 94: 49 Kameda Tsunenori, Teramoto Hidet
Page 95 and 96: 85 Matsuyama Shuichi, Ohkura Satosh
Page 97 and 98: 120 Ozawa Kenjiro, Kawahigashi Hiro
Page 99 and 100: 155 Taguchi-Shiobara Fumio, Yamamot
Page 101 and 102: 192 Ueno Osamu, Agarie Sakae, (2005
Page 103 and 104:
8 Murakami Ritsuko, Koyama Akio, Sh
Page 105 and 106:
20 Todoroki Junichi, Kaneko Hiroyuk
Page 107 and 108:
Executive Members and Research Staf
Page 109 and 110:
Developmental Biology Department Di
Page 111 and 112:
Research Leader Hayasaka Shoji Rese
Page 113 and 114:
Mutation Genetics Laboratory Head N
Page 115 and 116:
thousands of yen TOTAL BUDGET OPERA
Page 117:
Annual Report 2006 (Apr. 2005Mar. 2
show all

Annual Report 2006

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?