Annual Report 2006

More documents

Recommendations

Info

ecipient aborted two fetuses on day 61 and the other recipient delivered one live piglet (Fig. 6). Genomic PCR of DNA extracted from the piglet and the aborted fetuses confirmed the presence of EndoGalC gene. The FACS analysis showed that the levels of elimination of αGal epitope in the fibroblasts collected from the piglet and the aborted two fetuses were 98.3%, 99.0% and 99.2% respectively. The cloned piglet had no physical abnormalities and reached normal maturity. These results clearly showed that the combination of FACS with nuclear transfer has much superiority to produce transgenic pigs like our previous success of cloned pig with highly expression of human decay accelerating factor (hDAF). Moreover, we are planning to produce transgenic pigs with both hDAF and EndoGalC expression by mating with each transgenic cloned pig for xenotransplantation. Fig. 6 Cloned transgenic pig expressing EndoGalC for elimination of αGal epitope from follicle cells of the silkworm at late pupal state with high reactivity with JH was phospholylated by incubation with methoprene for 5 min. (2) New prothoracicostatic factors, FMRFamide (BRFa) were identified in . BRFa is predominantly expressed in neurosecretory cells of thoracic ganglia, and the neurons in prothoracic ganglia innervate the prothoracic glands to supply the peptides to the gland surface (Fig. 7). These results suggest that BRFa is controlling the activity of prothoracic gland by direct innervation. (3) We analyzed transactivation abilities of three isoforms of a metamorphosis-specific transcriptional factor Broad-complex (BR-C) isolated from by luciferase gene assay using a cell line. BR-C Z2 isoform that processes zinc finger DNA binding motives induced luciferase expression via either of three metamorphosis-specific gene promoters, but BR-C NZ1 and NZ4 isoforms without zinc finger motif did not, suggesting that BR-C isoforms with zinc finger motives play an important role for induction of multiple metamorphosis-specific genes. (4) We cultured pieces of larval integument in MGM-450 medium with 10 % fetal bovine serum (FBS). Reduced form glutathione was added in the medium to inhibit melanization of medium and integument. The pieces of integument were put on the supports of polyester membrane in order to expose air on surface of cuticle. At 48 hours after initiation of the culture we observed malformed cells at surround of the wound, but recognized only a few melanizing or damaged Regulation of Silkworm Larval Development The regulatory mechanisms on insect development, such as ecdysis and metamorphosis are being studied on the silkworm at Insect Growth Regulation Laboratory. Recent progresses are as follows; (1) A protein of cultured cells derived from silkworm ovary, which was phospholylated by methoprene, a juvenile homone (JH) analog, was MAP kinase, and it was also observed that the MAP kinase Fig. 7 BRFa-immunoreactive axons on the surface of prothoracic glands
cells. The epidermal cells maintained apparently cell growth activity on the culture condition for the immunofluorecent staining of incorporated bromodeoxyuridine (BrdU). These observations suggested that the integument culture was available method for study of growth and development on epidermal cells. Identification of new molecules in bovine placenta: Prolactin-related proteins and BCL2A1 Prolactin-related proteins (PRPs) are specific proteins of the growth hormone/ prolactin (GH/PRL) family in bovine placenta. A full-length cDNA for two new members of bovine PRPs, bPRP-VIII and -IX were identified, and their placental localization and quantitative expression were examined. New bPRP-VIII and -IX were identified from bovine placentome. Localization and quantitative gene expression in the placenta were respectively investigated by hybridization and real-time RT-PCR methods. Recombinant proteins of these genes were produced by a mammalian HEK293 cell expression system. Full-length bPRP-VIII and - IX cDNA were respectively cloned with 909 and 910 nucleotide open-reading-frames corresponding to proteins of 236 and 238 amino acids. The predicted bPRP-VIII amino acid sequence shared about 40 to 70% homology with other bPRPs, and bPRP-IX had about 50 to 80% homology of others. The two new bPRPs were detected only in the placenta by RT-PCR. mRNA was primarily expressed in the cotyledon and intercotyledonary tissues throughout gestation. An hybridization analysis revealed the presence of bPRP-VIII and -IX mRNA in the trophoblastic binucleate and /or trinucleate cells. bPRP-VIII mRNA was observed in the extra-embryonic membrane on Day 27 of gestation, however, no bPRP-IX mRNA was observed in the extra-embryonic membrane in the same stage of pregnancy by quantitative real-time RT-PCR analysis. Both new bPRP genes were possible to translate a mature protein in a mammalian cell expression system with approximately 28 kDa in bPRP- VIII and 38 kDa in bPRP-IX. Their different temporal and spatial expressions suggest a different role for these genes in bovine placenta during gestation. A full-length cDNA for the bovine BCL2 antiapoptotic family member, BCL2-related protein A1 (BCL2A1) was identified. Spaciotemporal expression profiles of BCL2A1 suggested the implication to trophoblast cell proliferation and differentiation during pregnancy. We cloned a full-length bovine BCL 2A1 cDNA with 725 nucleotides and an openreading frame corresponding to a protein of 175 amino acids. The predicted amino acid sequence shared 78% homology with human BCL2A1. All BCL2 homology domains (BH1, BH 2, BH3, and BH4) in bovine BCL2A1 were conserved as in other mammalian BCL2A1. In Fig. 8 hybridization for BCL2A1, BAX, CASP3 and PL in bovine placenta
Page 1 and 2: 2006
Page 3 and 4: Teruo Ishige, President The Nationa
Page 5 and 6: List of Publication Original paper
Page 7 and 8: Topics of Research in This Year Pos
Page 9 and 10: studies. To construct a fully satur
Page 11 and 12: Fig. 2 Geographical distribution of
Page 13 and 14: (ABR0015, ABR0257, ABR0417, ABR0495
Page 15 and 16: Mulberry latex defense mulberry tre
Page 17 and 18: Fig. 2 Growth inhibitory effects of
Page 19 and 20: Fig. 3 Change of the levels of luci
Page 21 and 22: Next, we examined the effect of tra
Page 23 and 24: G enome Research Department Introdu
Page 25 and 26: The Rice Annotation Project Databas
Page 27 and 28: industrial and agricultural concern
Page 29 and 30: One of the main problems involving
Page 31 and 32: Fig. 3 Mapping of QTL for rachis in
Page 33 and 34: two genes, XOO2263 and XOO4134 cont
Page 35 and 36: G enebank Genebank Project The NIAS
Page 37 and 38: using dormant winter buds. Also, we
Page 39 and 40: Insect and Animal Sciences Division
Page 41 and 42: Expression patterns of two novel ge
Page 43: Fig. 5 PGC and EBC fused cells PGCs
Page 47 and 48: Animal and cell culture models for
Page 49 and 50: terminus, respectively. A signal se
Page 51 and 52: Fig. 2 Trehalose content of body pa
Page 53 and 54: Fig. 6 Birth weight of 11 adult SNT
Page 55 and 56: The role of glucose as a metabolic
Page 57 and 58: analysis using microsatellite marke
Page 59 and 60: Fig. 2 Recorded Electromyograms (le
Page 61 and 62: Table 1 Menu of Commercial silkworm
Page 63 and 64: Fig. 3 Expression of GFP gene in th
Page 65 and 66: also surveyed in the genetic stocks
Page 67 and 68: esearch were located in or near gen
Page 69 and 70: elatives results in severe reductio
Page 71 and 72: and circadian clocks may control th
Page 73 and 74: B iochemistry Department Structural
Page 75 and 76: Proteomic analysis Based on the pro
Page 77 and 78: photoautotrophic medium (Fig. 1B).
Page 79 and 80: Fig. 4 Blast resistance of OsWRKY45
Page 81 and 82: phosphatase activity by SIPK depend
Page 83 and 84: the endosperm in co-operation with
Page 85 and 86: isolated genes in transgenic Anal
Page 87 and 88: Fig. 1 Gene structures and mutation
Page 89 and 90: cytochimera among the mulberries (
Page 91 and 92: 15 G. Hariraj, Kinoshita Haruo, T.
Page 93 and 94: 49 Kameda Tsunenori, Teramoto Hidet
Page 95 and 96:
85 Matsuyama Shuichi, Ohkura Satosh
Page 97 and 98:
120 Ozawa Kenjiro, Kawahigashi Hiro
Page 99 and 100:
155 Taguchi-Shiobara Fumio, Yamamot
Page 101 and 102:
192 Ueno Osamu, Agarie Sakae, (2005
Page 103 and 104:
8 Murakami Ritsuko, Koyama Akio, Sh
Page 105 and 106:
20 Todoroki Junichi, Kaneko Hiroyuk
Page 107 and 108:
Executive Members and Research Staf
Page 109 and 110:
Developmental Biology Department Di
Page 111 and 112:
Research Leader Hayasaka Shoji Rese
Page 113 and 114:
Mutation Genetics Laboratory Head N
Page 115 and 116:
thousands of yen TOTAL BUDGET OPERA
Page 117:
Annual Report 2006 (Apr. 2005Mar. 2
show all

Annual Report 2006

Create successful ePaper yourself

Delete template?

Save as template?