Annual Report 2006

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Fig. 1 Phylogenetic relationships among species A strict consensus tree was calculated using the maximum parsimony method based on the sequence data of 18 chloroplast DNA regions. Tree length = 264, confidence interval =0.8659, retention index =0.9299, rescaled consistency index =0.8912. Species names and abbreviations of each accession (in parentheses) are shown. If two accessions of the same species have identical sequences, they are shown as 2 acc. The numbers above the nodes represent bootstrap values expressed as the percentage of 1,000 bootstrap replications. and form an independent clade; (2) has a paraphyletic relationship with the other five species; and (3) no or very low intraspecific variation was observed in and whereas higher intraspecific variation was observed in Based on the number of nucleotide substitutions, the divergence time between and and between and maize were calculated to be about 730-780 thousand years ago and about 5.9 million years ago, respectively. These results suggest that the cytoplasm of species are very closely related. Analysis of a large seeded mutant in black gram The Asian consists of domesticated species among them is black gram. A mutant of this species has a seed size about double that of traditional cultivated black gram and six times the size of wild black gram (Fig. 2a). This mutant is also larger in other organs such as leaves (Fig. 2b). In order to determine the location of this mutation(s) on the black gram genome, a genome map was developed based on a cross between the mutant black gram and Fig. 2 Variation in (a) seeds and (b) leaves of mutant, cultivated and wild black gram wild black gram. In a BC1F1 population of 180 individuals from this cross 148 marker loci could be assigned to the 11 linkage groups that corresponds to the haploid chromosome number of black gram. This is the first genetic linkage map for black gram and it was compared with that of a close relative, azuki bean. Inversions, insertions, deletions / duplications and a translocation were detected between the black gram and azuki bean linkage maps. Domestication trait QTL, including seed size, locations have been identified on the genome map of black gram. Identification and mapping of QTL for rachis internode length associated with cleistogamy in barley General knowledge of the closed flowering trait, or cleistogamy, of barley, L., is still limited. We studied the relationship between cleistogamy and characters of spike morphology and detected a linkage of
Fig. 3 Mapping of QTL for rachis internode length () on the long arm of chromosome 2H of barley A: Genetic map of 126 DH lines of Mikamo Golden x Harrington; B: Genetic map of 150 F2 plants of Misato Golden x Satsuki Nijo. The genetic distance between markers is given in cM. Arrows represent positions; shaded bars represent 90% confidence interval of cleistogamy genes with a highly significant quantitative trait locus (QTL) for rachis internode length on the long arm of chromosome 2H(Fig.3). The mapping populations consisted of 129 doubled haploid lines of Mikamo Golden Harrington and 150 F2 plants of Misato Golden Satsuki Nijo. The phenotypic variance explained by this QTL accounted for 77.5% and 82.6% of the variance in rachis internode length in these two populations. The peaks of the QTL coincided with the positions of the cleistogamy gene loci. Development of GCP domain model The Generation Challenge Programme (GCP) aims to assist farmers in the developing world by using advances in molecular biology to harness the rich global heritage of plant genetic resources and create a new generation of crops that meet the needs of resource-poor people. Crop research within the GCP has many interrelated data types, focused on germplasm. The scope of the GCP scientific domain model is to cover this range of data types spanning germplasm, phenotype, genotype, mapping, functional genomics and location environment data. NIAS has a role to develop the functional genomics sub-domain model. Software implementations of the GCP domain model will form the middleware of the GCP platform and network that will link and user tools and interfaces to local and remote (web service connected) data source (Fig. 4). The model is documented in Unified Modelling Language (UML). Computable versions of the UML model are published in the Demeter package of the GCP Middleware project in CropForge (http:// cropforge. irri. org/ projects/ gcpmiddleware / ) . The alpha release of the domain model is also published to http://www.generationcp.org/ model. Fig. 4 Relationship of GCP domain model to platform
Page 1 and 2: 2006
Page 3 and 4: Teruo Ishige, President The Nationa
Page 5 and 6: List of Publication Original paper
Page 7 and 8: Topics of Research in This Year Pos
Page 9 and 10: studies. To construct a fully satur
Page 11 and 12: Fig. 2 Geographical distribution of
Page 13 and 14: (ABR0015, ABR0257, ABR0417, ABR0495
Page 15 and 16: Mulberry latex defense mulberry tre
Page 17 and 18: Fig. 2 Growth inhibitory effects of
Page 19 and 20: Fig. 3 Change of the levels of luci
Page 21 and 22: Next, we examined the effect of tra
Page 23 and 24: G enome Research Department Introdu
Page 25 and 26: The Rice Annotation Project Databas
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Page 29: One of the main problems involving
Page 33 and 34: two genes, XOO2263 and XOO4134 cont
Page 35 and 36: G enebank Genebank Project The NIAS
Page 37 and 38: using dormant winter buds. Also, we
Page 39 and 40: Insect and Animal Sciences Division
Page 41 and 42: Expression patterns of two novel ge
Page 43 and 44: Fig. 5 PGC and EBC fused cells PGCs
Page 45 and 46: cells. The epidermal cells maintain
Page 47 and 48: Animal and cell culture models for
Page 49 and 50: terminus, respectively. A signal se
Page 51 and 52: Fig. 2 Trehalose content of body pa
Page 53 and 54: Fig. 6 Birth weight of 11 adult SNT
Page 55 and 56: The role of glucose as a metabolic
Page 57 and 58: analysis using microsatellite marke
Page 59 and 60: Fig. 2 Recorded Electromyograms (le
Page 61 and 62: Table 1 Menu of Commercial silkworm
Page 63 and 64: Fig. 3 Expression of GFP gene in th
Page 65 and 66: also surveyed in the genetic stocks
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Page 69 and 70: elatives results in severe reductio
Page 71 and 72: and circadian clocks may control th
Page 73 and 74: B iochemistry Department Structural
Page 75 and 76: Proteomic analysis Based on the pro
Page 77 and 78: photoautotrophic medium (Fig. 1B).
Page 79 and 80: Fig. 4 Blast resistance of OsWRKY45
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phosphatase activity by SIPK depend
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the endosperm in co-operation with
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isolated genes in transgenic Anal
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Fig. 1 Gene structures and mutation
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cytochimera among the mulberries (
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15 G. Hariraj, Kinoshita Haruo, T.
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49 Kameda Tsunenori, Teramoto Hidet
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85 Matsuyama Shuichi, Ohkura Satosh
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120 Ozawa Kenjiro, Kawahigashi Hiro
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155 Taguchi-Shiobara Fumio, Yamamot
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192 Ueno Osamu, Agarie Sakae, (2005
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8 Murakami Ritsuko, Koyama Akio, Sh
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20 Todoroki Junichi, Kaneko Hiroyuk
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Executive Members and Research Staf
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Developmental Biology Department Di
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Research Leader Hayasaka Shoji Rese
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Mutation Genetics Laboratory Head N
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thousands of yen TOTAL BUDGET OPERA
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Annual Report 2006 (Apr. 2005Mar. 2
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Annual Report 2006

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