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RAPID AND SIMPLE ANALYSIS OF ALLICIN IN ALLIUM SPECIES BY LC-CIS-MS/MS and CL 659750) was deposited at the Herbarium of “A. Borza” Botanical Garden, “Babes-Bolyai” University of Cluj-Napoca. Before analysis, the vegetal extracts were diluted 10 folds with distilled water, then 1 µL was injected in the chromatographic system. Method validation In three different days, a calibration curve was run. The linearity of the peak area against standard concentration was verified between 18-864 µg/mL ALC by applying least-squares linear regression. The applied calibration model was y = a*x+b, 1/y weight, where y is peak area and x, concentration. Distribution of the residuals (% difference of the back-calculated concentration from the nominal concentration) was investigated. The calibration model was accepted if the residuals were within ±20% at the lower limit of quantification and within ±15% at all other calibration levels and at least 2/3 of the standards meet this criterion [13-15]. The limit of quantification was established as the lowest calibration standard with an accuracy and precision less than 20%. ACKNOWLEDGMENTS This work was supported by CNCSIS–UEFISCSU, project PNII–IDEI code 2272/2008. REFERENCES 1. C. Nencini, F. Cavallo, A. Capasso, G.G. Franchi, G. Giorgio, L. Micheli, Phytother. Res., 2007, 21(9), 874. 2. D. Stajner, J. Canadanovic-Brunet, A. Pavlovic, Phytother. Res., 2004, 18, 522. 3. H. Amagase, B.L. Petesch, H. Matsuura, S. Kasuga, Y. Itakura, J. Nutr., 2001, 131, 955S. 4. C.A. Newall, A.A. Anderson, J.D. Phillipson, "Herbal Medicines. A guide for Healthcare Professionals", The Pharmaceutical Press, London, 1996. 5. M. Parvu, A.E. Parvu, O. Rosca-Casian, L. Vlase, G. Groza, J. Med. Plant. Res., 2010, 4(2), 138. 6. I. Arnault, J.P. Christides, N. Mandon, T. Haffner, R. Kahane, J. Auger, J. Chromatogr. A, 2003, 991, 69. 7. K. Baghalian, S.A. Ziai, M.R. Naghavi, H.N. Badi, A. Khalighi, Scientia Horticulturae, 2005, 103, 155. 8. P. Bocchini, C. Andalo, R. Pozzi, G.C. Galletti, A. Antonelli, Anal. Chim. Acta, 2001, 441, 37. 9. E.M. Calvey, K.D. White, J.E. Matusik, D. Sha, E. Block, Phytochem., 1998, 38(1), 248. 10. M. De Diego, M. Avello, S. Mennickent, M. Fernandez, P. Fernandez, J. Sep. Sci., 2007, 30, 2703. 303
L. VLASE, M. PÂRVU, A. TOIU, E. A. PÂRVU, S. C. COBZAC, M. PUŞCAŞ 11. A. Medvedovici, K. Lazou, A. d'Oosterlinck, Y.N. Zhao, P. Sandra, J. Sep. Sci., 2002, 25, 173. 12. E. Block, "Garlic and Other Alliums: The Lore and the Science". The Royal Society of Chemistry, Cambridge, 2009. 13. D. Mihu, L. Vlase, S. Imre, C.M. Mihu, M. Achim, D.L. Muntean, <strong>Studia</strong> Univ. Babeş-Bolyai, Chemia, 2009, 54( 3), 151. 14. M. Achim, D. Muntean, L. Vlase, I. Bâldea, D. Mihu, S.E. Leucuţa, <strong>Studia</strong> Univ. Babeş- Bolyai, Chemia, 2009, 54(3), 7. 15. D.S. Popa, L. Vlase, S.E. Leucuţa, F. Loghin, Farmacia, 2009, 57(3), 301. 304
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CHEMIA 4/2010
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L.M. PĂCUREANU, A. BORA, L. CRIŞA
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Studia Universitatis Babes-Bolyai C
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MIRCEA V. DIUDEA theorem in multi-s
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MIRCEA V. DIUDEA 4. M.V. Diudea, Cs
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STUDIA UBB. CHEMIA, LV, 4, 2010 DIA
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DIAMOND D 5 , A NOVEL ALLOTROPE OF
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MONICA L. POP, MIRCEA V. DIUDEA AND
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STUDIA UBB. CHEMIA, LV, 4, 2010 EVA
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EVALUATION OF THE ANTIOXIDANT CAPAC
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STUDIA UBB. CHEMIA, LV, 4, 2010 TO
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TO WHAT EXTENT THE NMR “MOBILE PR
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LORENTZ JÄNTSCHI, SORANA D. BOLBOA
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STUDIA UBB. CHEMIA, LV, 4, 2010 DIA
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DIAGNOSTIC OF A QSPR MODEL: AQUEOUS
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STUDIA UBB. CHEMIA, LV, 4, 2010 MOD
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MODELING THE BIOLOGICAL ACTIVITY OF
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MODELING THE BIOLOGICAL ACTIVITY OF
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LILIANA M. PĂCUREANU, ALINA BORA,
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A. R. ASHRAFI, P. NIKZAD, A. BEHMAR
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GHOLAM HOSSEIN FATH-TABAR, ALI REZA
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GHOLAM HOSSEIN FATH-TABAR, ALI REZA
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MODJTABA GHORBANI symmetric but it
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MODJTABA GHORBANI group can be comp
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MODJTABA GHORBANI 10. P.V. Khadikar
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HOSSEIN SHABANI, ALI REZA ASHRAFI,
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MIRCEA V. DIUDEA, CSABA L. NAGY, PE
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STUDIA UBB. CHEMIA, LV, 4, 2010 WIE
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WIENER INDEX OF MICELLE-LIKE CHIRAL
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WIENER INDEX OF MICELLE-LIKE CHIRAL
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STUDIA UBB. CHEMIA, LV, 4, 2010 TUT
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TUTTE POLYNOMIAL OF AN INFINITE CLA
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TUTTE POLYNOMIAL OF AN INFINITE CLA
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ALI REZA ASHRAFI, HOSSEIN SHABANI,
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MOHAMMAD A. IRANMANESH, A. ADAMZADE
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RALUCA O. POP, MIHAI MEDELEANU, MIR
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MELINDA E. FÜSTÖS, ERIKA TASNÁDI
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STUDIA UBB. CHEMIA, LV, 4, 2010 APP
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APPLICATION OF NUMERICAL METHODS IN
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APPLICATION OF NUMERICAL METHODS IN
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VLADIMIR R. ROSENFELD, DOUGLAS J. K
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M. GHORBANI, M. A. HOSSEINZADEH, M.
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STUDIA UBB. CHEMIA, LV, 4, 2010 CLU
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CLUJ CJ POLYNOMIAL AND INDICES IN A
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CLUJ CJ POLYNOMIAL AND INDICES IN A
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