Botryodiplodia sp. - Crops for the Future

More documents

Recommendations

Info

Figure 21: Soil sampling in a rambutan plantation In this manner 16 soil samples were taken from 4 different rambutan plantations and 13 samples from annona trees from 3 different areas. 7 of the “annona samples” were taken from Annona squamosa trees and 6 from Annona muricata trees; all the trees were standing in private gardens or little home gardens. Table 1 gives an overview of the soil samples and their place of sourcing. Table 1: An Overview of the soil samples which were taken District City Gampaha District Urapola Quantity soil samples of: rambutan A. squamosa A. muricata 1 st plantation 4 2 nd plantation 4 3 rd plantation 4 Date 3. May Kegalle District Warakapola 4 8. June Anuradhapura Wijayapura 2 1 10. May District Medawachchiya 3 11. May Kegalle District Galagedera 1 12. May Matale District Matale 2 4 26. May 9.1.3 Isolation of the bio-control agent To find the bio-control agent in the soil samples you have to see total number of fungi which are present in the soil. If Trichoderma sp. is present within the fungi an isolation can be done. 26
The following materials and chemicals are needed to analyse a soil sample regarding the population of the fungi: Materials: • Bunsen burner • 2 flasks • Funnel carped with cotton wool • 6 closable tubes • Micropipette for 1 ml with 20 pipette-tips • Petri-dishes filled with PDA Chemicals: • 10 g soil • 150 ml distilled water To analyse a soil sample a spore suspension has to be done before. Therefore 10 g of the soil sample was placed in a flask to which 10 ml distilled water was added and shaken carefully in a circular movement for 20 minutes. The solution was then filtered through a funnel with cotton wool into a second flask and in doing so the spore solution was obtained. Normally the spore concentration of the spore solution is too high and a dilution series has to be done. Therefore 1 ml of the spore solution was used with 9 ml distilled water to obtain a 10 -1 dilution. This dilution procedure was repeated until a dilution series with the powers 10 -1 to 10 -6 was obtained. 0.1 ml of each dilution was spread on petridishes filled with PDA and incubated. These cultures (Figure 22) were observed every day for one week to check weather Trichoderma sp. is appearing somewhere. If the existence of the wanted fungus (Trichoderma sp.) was speculated an inspection by using the microscope was undertaken. If the identification was positive an isolation of the fungus to a pure culture was undertaken. Figure 22: Seven days old plate from a soil experiment 27
Page 1: Control of Post Harvest Disease (Bo
Page 4 and 5: List of Figures Figure 1: The Sri L
Page 6 and 7: 1 Summary Experiments were undertak
Page 8 and 9: 3 Sri Lanka The Democratic Socialis
Page 10 and 11: 3.2 Climate The climate of Sri Lank
Page 12 and 13: valuable consultations in industria
Page 14 and 15: Rambutan trees are either dioecious
Page 16 and 17: However, the most important genus i
Page 18 and 19: 6.2.1.2 Annona muricata Annona muri
Page 20 and 21: Figure 9: Annona squamosa Figure 10
Page 22 and 23: Characteristically for this fungus
Page 24 and 25: Figure 15: Phialides of Trichoderma
Page 26 and 27: autoclave is used for liquid steril
Page 30 and 31: 9.1.4 Isolation of the pathogen Mat
Page 32 and 33: The effectivity of the BC-agent was
Page 34 and 35: Figure 29: Experimental series of a
Page 36 and 37: Two of the Trichoderma sp. with a h
Page 38 and 39: Results of the sporulation experime
Page 40 and 41: 12 References ALAM, M.S., BEGUM, M.
Page 42 and 43: ONG, P.K.C., ACREE, T.E. and LAVIN,
Page 44 and 45: Figure 36: List of bio-control agen
Page 46: Tmu III Galagedera • Mycelial gro

Botryodiplodia sp. - Crops for the Future

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?