Botryodiplodia sp. - Crops for the Future

Two of the Trichoderma sp. with a high effectiveness were isolated from rambutan
plantations in Warakapola. The third of the high active ones was found in a soil sample taken
from an Annona muricata tree in Wijayapura. The fungi which were isolated from soil
samples taken from Annona squamosa trees showed the lowest activities against
Botryodiplodia sp.. Only one of these fungi had a moderate effectiveness.
Table 4 presents the results of the bio-assay, whereas “+++” identifies a good, “++” a
moderate, “+” a low and “0” no effectiveness against the pathogens.
Table 4: Results of the effectiveness of the isolated Trichoderma sp.
Code Source Activity
Tra I Urapola ++
Tra II Warakapola +++
Tra III Warakapola +++
Tsq I Matale 0
Tsq II Matale +
Tsq III Wijayapura ++
Tsq IV Medawachchiya 0
Tmu I Galagedera ++
Tmu II Galagedera ++
Tmu III Galagedera 0
Tmu IV Matale +
Results of the cross-inoculation experiment:
To interpret the results of the experiments with their replications as overall results T-tests
were undertaken. In all tests the null hypothesis could be disproved.
All pathogens have shown the same severity on the rambutan fruits after three days (Figure
32). In the first two days the severity which was caused by the pathogen “Bmu I” was higher.
Cross-inoculationNephelium lappaceum
Severity [%]
100
80
60
40
20
0
Day 1 Day 2 Day 3 Day 4 Day 5
Time after inocculation [d]
Bmu I Bsq IV Bra III Bsq III Control I Control II
Figure 32: Results of the cross-inoculation on rambutan
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