Botryodiplodia sp. - Crops for the Future

Figure 29: Experimental series of a cross-inoculation on Annona squamosa
(day 2 to day 6)
9.1.7 Sporulation experiment
As mentioned in chapter 7 Botryodiplodia sp. under artificial conditions need a very long time
to produce spores, which are crucial for the identification of the fungus. Therefore two
different methods were tested which possibly affect the sporulation of Botryodiplodia sp. in
consideration of its speed.
The different Botryodiplodia sp. cultures were exposed to three different conditions.
First all cultures were subcultured nine times and incubated for five days at 28 °C to obtain
completely grown plates. Following the cultures were divided into three sets each with three
plates.
One set remained in the incubator, the second one was exposed to the daylight and the third
one was exposed to daylight after a cold shock was admitted by storing the culture plates for
seven days at 13 °C in a cold room.
The experimental time was one month while the cultures were analysed regarding to spores
twice a week.
9.2 Results
Trichoderma sp. found in the soil samples:
In total twelve Trichoderma sp. could be isolated from six sources, which were different
concerning their morphology and their development (Figure 30). The exact differences
between the several isolates are found in the Annexes. Table 2 gives an overview of the
isolated Trichoderma sp. regarding their source and the time they need to full sporulation of
the plate.
32