Botryodiplodia sp. - Crops for the Future

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The effectivity of the BC-agent was established by doing daily measurements which recorded the diameter of the pathogen between the wells. If the diameter of the pathogen was getting smaller during the experimental time or the pathogen disappeared completely, the BC-agent had a good activity. If the BC-agent had no activity against the pathogen the diameter of the pathogen increased and the BC-agent could not develop. Each experimental series was incubated for 7 days at a temperature of 28 °C and daily observed (Figure 25 and Figure 26). Figure 25: BC-agent with a good (right) and a bad (left) effectiveness Figure 26: Experimental series of bio-assay after 2 (left) and 6 (right) days 9.1.6 Cross-inoculation The cross-inoculation experiment is used as an examination if the pathogens which were isolated from the various fruits are host specific or if they are able to infect the other fruits with the same aggressiveness. 30
The experiment was carried out on rambutan, sugar apple and soursop fruits and repeated. In every experimental trial four pathogens were tested on ten fruits and two different controls were undertaken, in which likewise ten fruits were used. So that in total 60 fruits were necessary for one experimental series. The soursops and sugar apples were bought from local markets in Colombo whereas the rambutan fruits were picked direct from a rambutan plantation. The experiments undertaken with rambutan were only done with fruits of the variety `Malwana Special´. At the beginning of the experiments the fruits had to be surface sterilized to avoid field inoculations. Therefore the fruits were dipped for five minutes into a 5 % sodium hypochlorite solution. After drying the fruits on air in the laminar flow cabinet one well was cut near the stem by using a cork borer (No. 5). It was important that the well was not cut too deep into the fruit at best only the skin should be removed. Than a seven day old mycelia disk of the pathogen was insert into the well. In the first control (control 1) the wells were filled with distilled water whereas the second control (control 2) consisted out of untreated fruits. All fruits where stored in self-made humidity chambers (Figure 27) during the experimental time of six days. An observation of the fruits was undertaken daily which gathered the disease spread in percent by using an infestation key (Figure 28 and Figure 29). Figure 27: Humidity chamber for the rambutan Cross-inoculation experiment Figure 28: Infestation key for crossinoculation experiment with sugar apple 31
Page 1: Control of Post Harvest Disease (Bo
Page 4 and 5: List of Figures Figure 1: The Sri L
Page 6 and 7: 1 Summary Experiments were undertak
Page 8 and 9: 3 Sri Lanka The Democratic Socialis
Page 10 and 11: 3.2 Climate The climate of Sri Lank
Page 12 and 13: valuable consultations in industria
Page 14 and 15: Rambutan trees are either dioecious
Page 16 and 17: However, the most important genus i
Page 18 and 19: 6.2.1.2 Annona muricata Annona muri
Page 20 and 21: Figure 9: Annona squamosa Figure 10
Page 22 and 23: Characteristically for this fungus
Page 24 and 25: Figure 15: Phialides of Trichoderma
Page 26 and 27: autoclave is used for liquid steril
Page 28 and 29: Figure 21: Soil sampling in a rambu
Page 30 and 31: 9.1.4 Isolation of the pathogen Mat
Page 34 and 35: Figure 29: Experimental series of a
Page 36 and 37: Two of the Trichoderma sp. with a h
Page 38 and 39: Results of the sporulation experime
Page 40 and 41: 12 References ALAM, M.S., BEGUM, M.
Page 42 and 43: ONG, P.K.C., ACREE, T.E. and LAVIN,
Page 44 and 45: Figure 36: List of bio-control agen
Page 46: Tmu III Galagedera • Mycelial gro

Botryodiplodia sp. - Crops for the Future

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