Botryodiplodia sp. - Crops for the Future

List of Contents
List of Contents .......................................................................................................................... 1
List of Figures ............................................................................................................................ 2
List of Tables.............................................................................................................................. 3
List of Acronyms........................................................................................................................ 3
1 Summary ................................................................................................................................. 4
2 Introduction ............................................................................................................................. 5
3 Sri Lanka ................................................................................................................................. 6
3.1 Geography ........................................................................................................................ 6
3.2 Climate ............................................................................................................................. 8
3.3 Socio-economics .............................................................................................................. 8
4 The host institutes.................................................................................................................... 9
4.1 International Centre for Underutilised Crops (ICUC)...................................................... 9
4.2 Industrial Technology Institute (ITI)................................................................................ 9
5 Reason for research project ................................................................................................... 10
6 The plants .............................................................................................................................. 11
6.1 Rambutan (Nephelium lappaceum)................................................................................ 11
6.1.1 Description of the plant/fruit ................................................................................... 11
6.1.2 Main diseases .......................................................................................................... 13
6.1.3 Economics in Sri Lanka .......................................................................................... 13
6.2 Annona (Annona sp.)...................................................................................................... 13
6.2.1 Description of the plant/fruit ................................................................................... 14
6.2.1.1 Annona cherimola ................................................................................................ 14
6.2.1.2 Annona muricata .................................................................................................. 16
6.2.1.3 Annona squamosa................................................................................................. 17
6.2.1.4 Annona reticulata................................................................................................. 18
6.2.2 Main diseases .......................................................................................................... 19
6.2.3 Economics in Sri Lanka .......................................................................................... 19
7 The disease (Botryodiplodia sp.)........................................................................................... 19
7.1 Taxonomy....................................................................................................................... 19
7.2 Disease pattern ............................................................................................................... 21
8 Bio-control agent (Trichoderma sp.)..................................................................................... 21
8.1 Taxonomy....................................................................................................................... 21
8.2 The role of mycoparasitism............................................................................................ 22
9 Experiments........................................................................................................................... 23
9.1 Experimental procedure ................................................................................................. 23
9.1.1 Laboratory set-up ........................................................................................................ 23
9.1.2 Soil sampling........................................................................................................... 25
9.1.3 Isolation of the bio-control agent ............................................................................ 26
9.1.4 Isolation of the pathogen ......................................................................................... 28
9.1.5 Bio-Assays............................................................................................................... 29
9.1.6 Cross-inoculation..................................................................................................... 30
9.1.7 Sporulation experiment ........................................................................................... 32
9.2 Results ............................................................................................................................ 32
10 Conclusion........................................................................................................................... 37
11 Acknowledgement............................................................................................................... 37
12 References ........................................................................................................................... 38
13 Annexes............................................................................................................................... 41
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