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70 uicROScopic ruxGi.<br />

conclusIon_, careful examination was necessary^ and<br />

new modes of manipulation essential. The details<br />

of one metliod employed were to tlie following<br />

effect :— A sufficient number of spores were removed<br />

on tlie<br />

point of a lancet^ and placed on a slide in a<br />

di'op of alcoliol. Before <strong>the</strong> spu^t was quite evaporated_,<br />

two or three drops of strong<br />

nitric acid<br />

were added^ and <strong>the</strong> whole covered with thin glass.<br />

The slide was <strong>the</strong>n warmed over a spirit-lamp, <strong>the</strong><br />

acid not being allowed to boil, but only gradually<br />

heated to boiling point. By this means <strong>the</strong><br />

fruit was found to consist of an outer membrane,<br />

nearly transparent, and studded with tubercles;<br />

that this membrane enclosed a number of cells<br />

which constituted <strong>the</strong> apparent joints, and which<br />

were natm-ally flattened at ei<strong>the</strong>r end by mutual<br />

pressure.<br />

or ruptured, <strong>the</strong>se cells escaped,<br />

'VYhen <strong>the</strong> outer membrane was dissolved<br />

and became detached<br />

from each o<strong>the</strong>r. The cells, thus set free,<br />

exhibited a brownish or yellow ring around a paler<br />

area, in <strong>the</strong> interior of which an inner cell was<br />

to be sometimes marked with<br />

visible, sometimes globular, often irregular in shape.<br />

The examination of <strong>the</strong> ring was not entirely satisfactory;<br />

it appeared<br />

concentric lines having <strong>the</strong> appearance of wrinkles.<br />

The inner cell had granular contents and a central<br />

nucleus. "V^Qien perfectly free <strong>the</strong>y were spherical<br />

in form, with a distinct membrane of <strong>the</strong>ir own;<br />

and colourless, except when acted upon by reagents.<br />

The means employed to determine <strong>the</strong>

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