Biosafety in the laboratory - VIB

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Biosafety in the laboratory 3rd revised edition, May 2004 VIB publication, Flanders Interuniversity Institute for Biotechnology Editor: René Custers, regulatory affairs manager, VIB This booklet can be ordered from: VIB Rijvisschestraat 120 9052 Zwijnaarde, Belgium tel.: (09) 244 66 11 fax: (09) 244 66 10 e-mail: vib@vib.be web: http://www.vib.be Responsible publisher: Jo Bury, VIB Rijvisschestraat 120 9052 Zwijnaarde, Belgium Nothing from this publication may be copied and/or made public for commercial purposes. For educational purposes the source has to be mentioned. May 2004 FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY ©
PREFACE This is the third, revised edition of the booklet ‘Biosafety in the laboratory’. In this booklet you will find all the information that was already present in the earlier editions. Only a few alterations have been made, especially in the text concerning decontamination and inactivation. Modern biotechnology is a very important technology in the current biological and biomedical research. Thousands of researchers in the life sciences are using micro-organisms, plants or animals on a daily basis to answer relevant scientific questions. More and more use is made of genetically modified organisms. The scientific community itself has been the first to stress the importance of working safely with GMOs. Nowadays many regulations and guidelines exist for GMOs. These are designed to protect human health and the environment. We want to stress the importance of working safely and responsibly with biological and especially genetically modified material. It is the obligation of the researcher to apply all the necessary safety measures. It is also the obligation of the researcher to teach all inexperienced personnel the theoretical and practical aspects of biosafety. We hope that this booklet, together with practical instructions and guidance, will prove to be a useful tool to welcome people in a safe manner into the fascinating world of biotechnological laboratory research. Jo Bury and Rudy Dekeyser General management, VIB Preface 3
Page 1: FLANDERS INTERUNIVERSITY INSTITUTE
Page 5 and 6: 1. INTRODUCTION Biological material
Page 7 and 8: ates in weight units per kilogram b
Page 9 and 10: 3. CLASSIFICATION AND RISK ASSESSME
Page 11 and 12: Definition of a GMO GMOs are organi
Page 13 and 14: There is already quite some experie
Page 15 and 16: Routes of contamination Laboratory
Page 17 and 18: Animal and human cells Animal and h
Page 19 and 20: The spread of transgenic plants or
Page 21 and 22: Containment at the source As is the
Page 23 and 24: Guidelines for the proper use of a
Page 25 and 26: Room air Contaminated air HEPA-filt
Page 27 and 28: In addition to following these safe
Page 29 and 30: 6. CONTAMINATION, ACCIDENTS, DECONT
Page 31 and 32: • Sodium hypochlorite (bleach) Th
Page 33 and 34: The appropriate decontamination and
Page 35 and 36: 7. WORKING WITH COMMONLY USED LABOR
Page 37 and 38: therefore sufficient to check in av
Page 39 and 40: onment in a safety cabinet. Yeasts
Page 41 and 42: Working with genetically modified v
Page 43 and 44: Working with genetically modified A
Page 45 and 46: 8. REFERENCES Begrip voor Veilige M
Page 47 and 48: ANNEX 1: CONTAINMENT REQUIREMENTS I
Page 49 and 50: 3. Working practices and waste mana
Page 51 and 52: Specifications Containment level A1
Page 53 and 54:
Belgian requirements for G1-G3 grow
Page 55 and 56:
Specifications Containment levels G
Page 57 and 58:
The schemes should be used as follo
Page 59 and 60:
2. Activities with animal cells wit
Page 61 and 62:
4. Activities using plant cells Sec
Page 63 and 64:
CHAPTER C. GUIDELINES FOR THE CLASS
Page 65 and 66:
Human and animal pathogenic viruses
Page 67 and 68:
Fytopathogenic viruses P 2 Alfalfa
Page 69 and 70:
ANNEX 5: RESPONSIBLE PERSONS AND SO
Page 71:
SELFTEST ANSWERS Question 1: A gene
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