Biosafety in the laboratory - VIB

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3. Activities with animal cells in combination with viruses or viral vectors Second direction Activities in which genetic material is or has been added that has not been characterised Activities in which characterised genetic material is or has been added First direction 7. The viral vector is a complete or defective ecotropic mouse retrovirus 8. The viral vector is a human or animal pathogenic autonomously replicating infectious virus of respectively risk group 4, 3 or 2 or a defective virus that has been developed from it and the possibility that automously replicating virusparticles emerge does exist 9. The viral vector is, or has been developed from a satelite virus or is a defective virus developed from a human or animal pathogen of respectively risk group 4, 3 or 2; the possibility that automously replicating virusparticles emerge does not exist a. The donor produces a toxin of T3 L2 Resp. L4, L4, L4 Resp. L3, L3, L3 respectively class T2 L2 Resp. L4, L4, L3 Resp. L3, L2, L2 T1 L2 Resp. L4, L4, L3 Resp. L3, L2, L2 b. The donor is, or has been developed from, Risk group 4 L4 Resp. L4, L4, L4 Resp. L4, L4, L4 a for eucaryotic cells infectious virus of Risk group 3 L3 Resp. L4, L3, L3 Resp. L3, L3, L3 respectively risk group 4, 3 or 2, and the Risk group 2 L2 Resp. L4, L3, L2 Resp. L3, L2, L2 viral sequences that have been brought into the host can give rise to the formation of autonomously replicating virusparticles* c. The donor is, or has been developed from, Risk group 4 L3 Resp. L4, L3, L3 Resp. L3, L3, L3 a defect for eucaryotic cells infectious Risk group 3 L2 Resp. L4, L3, L2 Resp. L3, L2, L2 virus of respectively risk group 4, 3 or 2, Risk group 2 L1 Resp. L4, L3, L2 Resp. L3, L2, L1 and the viral sequences that have been brought into the host cannot give rise to the formation of autonomously replicating virusparticles* d. The donor is a non-viral pathogen of Risk group 4 L3 Resp. L4, L3, L3 Resp. L3, L3, L3 respectively Risk group 3 L2 Resp. L4, L3, L2 Resp. L3, L2, L2 Risk group 2 L1 Resp. L4, L3, L2 Resp. L3, L2, L1 e. The donor is an organism of biological risk L1 Resp. L4, L3, L2 Resp. L3, L2, L1 risk group 1, or a plant or an animal f. The sequence contains genetic T3 L2 Resp. L4, L4, L4 Resp. L3, L3, L3 information that codes for the production T2 L2 Resp. L4, L4, L3 Resp. L3, L2, L2 of a toxin of respectively class T1 L2 Resp. L4, L4, L3 Resp. L3, L2, L2 g. The sequence contains genetic Risk group 4 L4 Resp. L4, L4, L4 Resp. L4, L4, L4 information for the formation of a for Risk group 3 L3 Resp. L4, L3, L3 Resp. L3, L3, L3 eucaryotic cells infectious virus, of Risk group 2 L2 Resp. L4, L3, L2 Resp. L3, L2, L2 respectively risk group 4, 3 or 2 and the viral sequences that have been brought into the host can give rise to the formation of autonomously replicating virusparticles* h. The sequence contains genetic Risk group 4 L3 Resp. L4, L3, L3 Resp. L3, L3, L3 information for the formation of a defect Risk group 3 L2 Resp. L4, L3, L2 Resp. L3, L2, L2 for eucaryotic cells infectious virus, of Risk group 2 L1 Resp. L4, L3, L2 Resp. L3, L2, L1 respectively risk group 4, 3 or 2 and the viral sequences that have been brought into the host cannot give rise to the formation of autonomously replicating virusparticles* i. The sequence contains genetic L2 Resp. L4, L3, L2 Resp. L3, L2, L2 information that codes for a hazardous gene product, other than in f.** j. The sequence does not contain genetic L1 Resp. L4, L3, L2 Resp. L3, L2, L1 information that codes for a hazardous gene product * The risk classification may in some cases have to be higher than given above in the case of the formation of an infectious virusparticle with an increased host(cell)range or an increased virulence or pathogenicity. ** What constitutes a hazardous gene product is the most difficult risk classification question. Virulence gene products might be an example of a hazardous gene product. Final risk classification depends on the suspected effect of the gene product in the used hostorganism. 60 Biosafety in the laboratory
4. Activities using plant cells Second direction First direction 10. Activities in which characterised genetic material is or has been added 11. Activities in which genetic material is or has been added that has not been characterised Activities in which genetic material is or has been added that has not been characterised Activities in which characterised genetic material is or has been added a. The donor produces a toxin of T3 L2 L3 respectively class T2 L1 L2 T1 L1 L2 b. The donor is, or has been developed from, Risk group 4 L4 L4 a for eucaryotic cells infectious virus of Risk group 3 L3 L3 respectively risk group 4, 3 or 2, and the Risk group 2 L2 L2 viral sequences that have been brought into the host can give rise to the formation of autonomously replicating virusparticles c. The donor is, or has been developed from, Risk group 4 L1 L2 a defect for eucaryotic cells infectious virus of Risk group 3 L1 L2 respectively risk group 4, 3 or 2, and the viral Risk group 2 L1 L2 sequences that have been brought into the host cannot give rise to the formation of autonomously replicating virusparticles d. The donor is a non-viral pathogen of Risk group 4 L1 L2 respectively Risk group 3 L1 L2 Risk group 2 L1 L2 e. The donor is an organism of biological risk L1 L2 group 1, or a plant or an animal f. The sequence contains genetic information T3 L2 L3 that codes for the production of a toxin of T2 L1 L2 respectively class T1 L1 L2 g. The sequence contains genetic information Risk group 4 L4 L4 for the formation of a for eucaryotic cells Risk group 3 L3 L3 infectious virus, of respectively risk group 4, Risk group 2 L2 L2 3 or 2 and the viral sequences that have been brought into the host can give rise to the formation of autonomously replicating virusparticles h. The sequence contains genetic information Risk group 4 L1 L2 for the formation of a defect for eucaryotic Risk group 3 L1 L2 cells infectious virus, of respectively risk Risk group 2 L1 L2 group 4, 3 or 2 and the viral sequences that havebeen brought into the host cannot give rise to the formation of autonomously replicating virusparticles: i. The sequence contains genetic information L1 L2 that codes for a hazardous gene product, other than in f. j. The sequence does not contain genetic infor- L1 L2 mation that codes for a hazardous gene product Guidelines for the classification of GMO-activities 61
Page 1 and 2:
FLANDERS INTERUNIVERSITY INSTITUTE
Page 3 and 4:
PREFACE This is the third, revised
Page 5 and 6:
1. INTRODUCTION Biological material
Page 7 and 8:
ates in weight units per kilogram b
Page 9 and 10: 3. CLASSIFICATION AND RISK ASSESSME
Page 11 and 12: Definition of a GMO GMOs are organi
Page 13 and 14: There is already quite some experie
Page 15 and 16: Routes of contamination Laboratory
Page 17 and 18: Animal and human cells Animal and h
Page 19 and 20: The spread of transgenic plants or
Page 21 and 22: Containment at the source As is the
Page 23 and 24: Guidelines for the proper use of a
Page 25 and 26: Room air Contaminated air HEPA-filt
Page 27 and 28: In addition to following these safe
Page 29 and 30: 6. CONTAMINATION, ACCIDENTS, DECONT
Page 31 and 32: • Sodium hypochlorite (bleach) Th
Page 33 and 34: The appropriate decontamination and
Page 35 and 36: 7. WORKING WITH COMMONLY USED LABOR
Page 37 and 38: therefore sufficient to check in av
Page 39 and 40: onment in a safety cabinet. Yeasts
Page 41 and 42: Working with genetically modified v
Page 43 and 44: Working with genetically modified A
Page 45 and 46: 8. REFERENCES Begrip voor Veilige M
Page 47 and 48: ANNEX 1: CONTAINMENT REQUIREMENTS I
Page 49 and 50: 3. Working practices and waste mana
Page 51 and 52: Specifications Containment level A1
Page 53 and 54: Belgian requirements for G1-G3 grow
Page 55 and 56: Specifications Containment levels G
Page 57 and 58: The schemes should be used as follo
Page 59: 2. Activities with animal cells wit
Page 63 and 64: CHAPTER C. GUIDELINES FOR THE CLASS
Page 65 and 66: Human and animal pathogenic viruses
Page 67 and 68: Fytopathogenic viruses P 2 Alfalfa
Page 69 and 70: ANNEX 5: RESPONSIBLE PERSONS AND SO
Page 71: SELFTEST ANSWERS Question 1: A gene
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