Biosafety in the laboratory - VIB

3. Activities with animal cells in combination with viruses or viral vectors
Second direction
Activities in which genetic material is or has been added that has not been
characterised
Activities in which characterised genetic material
is or has been added
First direction
7. The viral vector is a complete or
defective ecotropic mouse
retrovirus
8. The viral vector is a human or
animal pathogenic autonomously
replicating infectious
virus of respectively risk group
4, 3 or 2 or a defective virus
that has been developed from it
and the possibility that automously
replicating virusparticles
emerge does exist
9. The viral vector is, or has been
developed from a satelite virus
or is a defective virus developed
from a human or animal pathogen
of respectively risk group 4,
3 or 2; the possibility that automously
replicating virusparticles
emerge does not exist
a. The donor produces a toxin of T3 L2 Resp. L4, L4, L4 Resp. L3, L3, L3
respectively class T2 L2 Resp. L4, L4, L3 Resp. L3, L2, L2
T1 L2 Resp. L4, L4, L3 Resp. L3, L2, L2
b. The donor is, or has been developed from, Risk group 4 L4 Resp. L4, L4, L4 Resp. L4, L4, L4
a for eucaryotic cells infectious virus of Risk group 3 L3 Resp. L4, L3, L3 Resp. L3, L3, L3
respectively risk group 4, 3 or 2, and the Risk group 2 L2 Resp. L4, L3, L2 Resp. L3, L2, L2
viral sequences that have been brought
into the host can give rise to the
formation of autonomously replicating
virusparticles*
c. The donor is, or has been developed from, Risk group 4 L3 Resp. L4, L3, L3 Resp. L3, L3, L3
a defect for eucaryotic cells infectious Risk group 3 L2 Resp. L4, L3, L2 Resp. L3, L2, L2
virus of respectively risk group 4, 3 or 2, Risk group 2 L1 Resp. L4, L3, L2 Resp. L3, L2, L1
and the viral sequences that have been
brought into the host cannot give rise to
the formation of autonomously
replicating virusparticles*
d. The donor is a non-viral pathogen of Risk group 4 L3 Resp. L4, L3, L3 Resp. L3, L3, L3
respectively Risk group 3 L2 Resp. L4, L3, L2 Resp. L3, L2, L2
Risk group 2 L1 Resp. L4, L3, L2 Resp. L3, L2, L1
e. The donor is an organism of biological risk L1 Resp. L4, L3, L2 Resp. L3, L2, L1
risk group 1, or a plant or an animal
f. The sequence contains genetic T3 L2 Resp. L4, L4, L4 Resp. L3, L3, L3
information that codes for the production T2 L2 Resp. L4, L4, L3 Resp. L3, L2, L2
of a toxin of respectively class T1 L2 Resp. L4, L4, L3 Resp. L3, L2, L2
g. The sequence contains genetic Risk group 4 L4 Resp. L4, L4, L4 Resp. L4, L4, L4
information for the formation of a for Risk group 3 L3 Resp. L4, L3, L3 Resp. L3, L3, L3
eucaryotic cells infectious virus, of Risk group 2 L2 Resp. L4, L3, L2 Resp. L3, L2, L2
respectively risk group 4, 3 or 2 and the
viral sequences that have been brought
into the host can give rise to the
formation of autonomously
replicating virusparticles*
h. The sequence contains genetic Risk group 4 L3 Resp. L4, L3, L3 Resp. L3, L3, L3
information for the formation of a defect Risk group 3 L2 Resp. L4, L3, L2 Resp. L3, L2, L2
for eucaryotic cells infectious virus, of Risk group 2 L1 Resp. L4, L3, L2 Resp. L3, L2, L1
respectively risk group 4, 3 or 2 and the
viral sequences that have been brought
into the host cannot give rise to the
formation of autonomously
replicating virusparticles*
i. The sequence contains genetic L2 Resp. L4, L3, L2 Resp. L3, L2, L2
information that codes for a hazardous
gene product, other than in f.**
j. The sequence does not contain genetic L1 Resp. L4, L3, L2 Resp. L3, L2, L1
information that codes for a hazardous
gene product
* The risk classification may in some cases have to be higher than given above in the case of the formation of an infectious virusparticle with an
increased host(cell)range or an increased virulence or pathogenicity.
** What constitutes a hazardous gene product is the most difficult risk classification question. Virulence gene products might be an example of a
hazardous gene product. Final risk classification depends on the suspected effect of the gene product in the used hostorganism.
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Biosafety in the laboratory