Biosafety in the laboratory - VIB

3. Working practices and waste management
Specifications
Containment level
A1 A2 A3 A4
34 Restricted access required required required (and control) required (and control)
35 Notification on the door: “Biohazard” required, except the required required required
symbol, coordinates of person in
“Biohazard” symbol
charge, containment level, type of
biological risk, list of persons who
have access, criteria for admittance
36 Animal housing to contain its own not required recommended required required
equipment
37 Protective clothing that is specific required required required, + optionally required, complete
for the containment zone appropriate shoe change of clothing
protection and footwear when
entering or leaving
38 Decontamination of the protective not required not required required required
clothing before it leaves the
containment zone
39 Gloves optional recommended required required
40 Mouth mask not required optional optional required
41 Face protection (eyes, mucosa) not required optional optional required
42 Generation of aerosols minimise minimise prevent prevent
43 Specific measures to prevent the spread not required recommended required required
of aerosols (including appropriate
equipment)
44 Mechanical pipetting required required required required
45 No drinking, eating or smoking, no use required required required required
of cosmetics, no manipulation of contacts,
or the stocking of consumables
46 Register in which all activities are notified required required required required
(transport of laboratory animals, inoculation
of GMM’s etc....)
47 Verification of control measures and required required required required
protective equipment
48 Notice on the use of decontaminants required required required required
49 Decontaminants in the siphons not required recommended required required
50 Instruction of the personnel required required required required
51 Written instructions on the biosafety required required required required
procedures
52 Efficient vector control (eg. for rodents recommended required required required
and insects)
53 Isolation of laboratory animals used required required required required
for experimentation (separate room) (separate room) (separate room)
54 In case of manipulation of zoopathogens, not required not required recommended, required,
period during which contact of the personnel period to be specified period to be specified
with the host animals should be avoided in the authorisation in the authorisation
WASTE MANAGEMENT
55 Inactivation of biological waste and/or biological required required required required
residues (contaminated cadavers, faeces 9 ) using an
appropriate and validated method before dumping
56 Inactivation of contaminated material required required required required
(glassware, cages, etc...) using an
appropriate and validated method before
reuse or destroying
57 Inactivation of the effluents of sinks and not required not required recommended required
showers using an appropriate and validated
method before dumping
9
Faeces from transgenic animals does not have to be inactivated
52
Biosafety in the laboratory