Biosafety in the laboratory - VIB

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Belgian requirements for A1-A4 animal houses 1. Equipment and technical requirements Specifications Containment level A1 A2 A3 A4 1 The animalarium is separated from not required required required required other working zones in the same building or is located in a separate building 2 Entry via an airlock not required recommended required required (with three compartments) 3 Doors that can be locked required required required required 4 Doors that close automatically not required required required required 5 Windows that cannot be opened not required not required, required required buth windows should (able to withstand be closed during breaking) experimentation 6 Airtight laboratory that enables not required optional required required decontamination with a gas 7 Building designed in such a way recommended required required required that accidental escape of animals is avoided 8 Observation window or equivalent recommended recommended required required system that enables one to see who is working in the laboratory 9 Decontamination facilities for the required (sinks) required (sinks) required (sinks near required (sinks and personnel the exit or in the shower, the latter fitted airlock) shower: with a chemical sprinkler recommended in case use is made of isolation suit fitted with positive air pressure) 10 Sinks that can be operated not required recommended required required non-manually 11 Coathooks or changing room for required required required required the protective clothing 12 Tubing fitted with a system that not required not required recommended required prevents backward flow of fluids 13 Separate room for the stocking of recommended required required required clean cages, feed and caging material 14 Surfaces resistant to decontaminants, required required required required impermeable and easy to clean (cages, benches) (cages, benches, (cages, benches, (cages, benches, floor) floor, walls, ceiling) floor, walls, ceiling) 15 Facility for the washing of cages required required required required 16 Automous electricity backup system not required not required recommended required 17 Fire alarm (not taking into account not required not required required required local fire regulations) 18 System that enables communication not required not required required required (nonwith the outside world manual operation) VENTILATION 19 Input airflowsystem separated from not required not required recommended required adjacent laboratories 20 Extract airflowsystem separated from not required optional recommended required adjacent laboratories 21 Input and extract airflowsystem not required optional required required interconnected to prevent accidental overpressure 22 Input and extract airflowsystem can be not required optional required required closed using valves 50 Biosafety in the laboratory
Specifications Containment level A1 A2 A3 A4 23 Negative airpressure in the not required optional required (control and required (control and controlled zone when compared alarm systems) alarm systems) to adjacent zones 24 HEPA-filtration of the air 6 not required optional required (on the required (on the inward extracted air airflow and double filtration on the extraced air) 25 System that allows changing of - - required required the filters without contamination of the environment 26 HEPA-filtered air may be recirculated - - optional forbidden 27 Specific measures to ventilate to such optional optional required 7 required an extent that contamination of the air is minimized 6 In case of use of virus not retained by HEPA filtration, special appropriate measures must be taken with regard to the extract air 7 Measures to be specified by the notifier and by the competent authorities 2. Safety equipment Specifications Containment level A1 A2 A3 A4 28 Microbiological safety cabinet not required optional optional optional (class I or II) (class I or II) (class III, or class II with the use of an isolation suit fitted with overpressure) 29 Animals in cages or equivalent optional optional optional optional isolation installations 30 Cages fitted with a HEPA filter not required optional required required 31 Autoclave on site in the building in the animalarium or in the animalarium an adjacent room 8 32 Double-ended autoclave not required not required recommended required 33 Fumigationsystem or not required recommended required required decontamination immersion bath 8 Transfer of material to an autoclave situated outside the laboratory should occur according to a validated method that guarantees an equivalent protection level. Containment requirements 51
Page 1 and 2: FLANDERS INTERUNIVERSITY INSTITUTE
Page 3 and 4: PREFACE This is the third, revised
Page 5 and 6: 1. INTRODUCTION Biological material
Page 7 and 8: ates in weight units per kilogram b
Page 9 and 10: 3. CLASSIFICATION AND RISK ASSESSME
Page 11 and 12: Definition of a GMO GMOs are organi
Page 13 and 14: There is already quite some experie
Page 15 and 16: Routes of contamination Laboratory
Page 17 and 18: Animal and human cells Animal and h
Page 19 and 20: The spread of transgenic plants or
Page 21 and 22: Containment at the source As is the
Page 23 and 24: Guidelines for the proper use of a
Page 25 and 26: Room air Contaminated air HEPA-filt
Page 27 and 28: In addition to following these safe
Page 29 and 30: 6. CONTAMINATION, ACCIDENTS, DECONT
Page 31 and 32: • Sodium hypochlorite (bleach) Th
Page 33 and 34: The appropriate decontamination and
Page 35 and 36: 7. WORKING WITH COMMONLY USED LABOR
Page 37 and 38: therefore sufficient to check in av
Page 39 and 40: onment in a safety cabinet. Yeasts
Page 41 and 42: Working with genetically modified v
Page 43 and 44: Working with genetically modified A
Page 45 and 46: 8. REFERENCES Begrip voor Veilige M
Page 47 and 48: ANNEX 1: CONTAINMENT REQUIREMENTS I
Page 49: 3. Working practices and waste mana
Page 53 and 54: Belgian requirements for G1-G3 grow
Page 55 and 56: Specifications Containment levels G
Page 57 and 58: The schemes should be used as follo
Page 59 and 60: 2. Activities with animal cells wit
Page 61 and 62: 4. Activities using plant cells Sec
Page 63 and 64: CHAPTER C. GUIDELINES FOR THE CLASS
Page 65 and 66: Human and animal pathogenic viruses
Page 67 and 68: Fytopathogenic viruses P 2 Alfalfa
Page 69 and 70: ANNEX 5: RESPONSIBLE PERSONS AND SO
Page 71: SELFTEST ANSWERS Question 1: A gene

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