Biosafety in the laboratory - VIB

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ANNEX 4: AKNOWLEDGEMENT We hereby show our gratitude to the following persons that have made useful comments to earlier editions of this booklet. Ann Van Gysel: communication manager VIB Bernadette Van Vaerenbergh, WIV, Section Biosafety and Biotechnology Greet Van Eetvelde: environmental coordinator, University of Ghent Huub Schellekens, platform committee VIB, University of Utrecht Kim De Rijck Mieke Van Lijsebettens, VIB department of Plant Genetics, University of Ghent Hilde Revets, VIB department of Molecular and Cellular Interactions, Free University of Brussels Peter Brouckaert, VIB department Molecular Biomedical Research, University of Ghent Thierry Vandendriessche, VIB department Transgene Technology and Gene Therapy, Catholic University of Leuven Wim van de Ven, VIB department Human Genetics, Catholic University of Leuven Hubert Backhovens, VIB department Molecular Genetics, University of Antwerp 68 Aknowledgement
ANNEX 5: RESPONSIBLE PERSONS AND SOURCES Names and coordinates of responsible persons The list below is meant to serve as an overview of the persons responsible for the safety in biotechnological laboratories. These persons differ from one department to another. Fill out the list below and make sure that you have it within reach in your laboratory. Name Telephone Other Emergence number Laboratory responsible person First aid responsible person Location of first aid kit Biosafety coordinator Workers protection contact Company doctor Waste contact person Regulatory affairs manager, VIB René Custers 09 244 6611 rene.custers@vib.be If you have general questions about classifications or necessary containment measures, you should first contact your superior. And in case this does not give you a decisive answer, you should subsequently contact your groupleader, internal biosafety expert or external biosafety expert. Responsible persons and sources 69
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FLANDERS INTERUNIVERSITY INSTITUTE
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PREFACE This is the third, revised
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1. INTRODUCTION Biological material
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ates in weight units per kilogram b
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3. CLASSIFICATION AND RISK ASSESSME
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Definition of a GMO GMOs are organi
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There is already quite some experie
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Routes of contamination Laboratory
Page 17 and 18: Animal and human cells Animal and h
Page 19 and 20: The spread of transgenic plants or
Page 21 and 22: Containment at the source As is the
Page 23 and 24: Guidelines for the proper use of a
Page 25 and 26: Room air Contaminated air HEPA-filt
Page 27 and 28: In addition to following these safe
Page 29 and 30: 6. CONTAMINATION, ACCIDENTS, DECONT
Page 31 and 32: • Sodium hypochlorite (bleach) Th
Page 33 and 34: The appropriate decontamination and
Page 35 and 36: 7. WORKING WITH COMMONLY USED LABOR
Page 37 and 38: therefore sufficient to check in av
Page 39 and 40: onment in a safety cabinet. Yeasts
Page 41 and 42: Working with genetically modified v
Page 43 and 44: Working with genetically modified A
Page 45 and 46: 8. REFERENCES Begrip voor Veilige M
Page 47 and 48: ANNEX 1: CONTAINMENT REQUIREMENTS I
Page 49 and 50: 3. Working practices and waste mana
Page 51 and 52: Specifications Containment level A1
Page 53 and 54: Belgian requirements for G1-G3 grow
Page 55 and 56: Specifications Containment levels G
Page 57 and 58: The schemes should be used as follo
Page 59 and 60: 2. Activities with animal cells wit
Page 61 and 62: 4. Activities using plant cells Sec
Page 63 and 64: CHAPTER C. GUIDELINES FOR THE CLASS
Page 65 and 66: Human and animal pathogenic viruses
Page 67: Fytopathogenic viruses P 2 Alfalfa
Page 71: SELFTEST ANSWERS Question 1: A gene
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