Biosafety in the laboratory - VIB

3. Working practices and waste management
Specifications
Containment level
L1 L2 L3 L4
31 Restricted access recommended required required (and required (and
control)
control)
32 Notification on the door: recommended required required required
a: “Biohazard” symbol (b, c) (a, b, c) (a, b, c, d, e, f) (a, b, c, d, e, f)
b: co-ordinates of the person in charge
c: containment level
d: type of biolocal risk
e: list of persons who have access
f: criteria for admittance
33 Laboratory to contain its own equipment not required not required required required
34 Protective clothing required required required required
(specific for the (specific for the
containment zone) 4 containment zone) 5
35 Decontamination of the protective not required not required recommended required
clothing before it leaves the
containment zone
36 Gloves not required optional required required
37 Mouth mask not required not required optional required
38 Face protection (eyes, mucosa) not required optional required required
39 Fysical containment of live recommended required required required
micro-organisms (closed systems)
40 Generation of aerosols minimize minimize prevent prevent
41 Specific measures to prevent the spread not required recommended required required
of aerosols (including appropriate
equipment)
42 Mechanical pipetting required required required required
43 No drinking, eating or smoking, no use required required required required
of cosmetics, no manipulation of
contacts, or the stocking of consumables
44 Appropriate laboratory registers required required required required
45 Verification of control measures and required required required required
protective equipment
46 Notice on the use of decontaminants required required required required
47 Decontaminants in the siphons not required not required recommended required
48 Instruction of the personnel required required required required
49 Written instructions on the biosafety required required required required
procedures
50 Efficient vector control not required recommended required required
(e.g. for rodents and insects)
51 Circulation of animals forbidden forbidden forbidden forbidden
52 In case of manipulation of zoopathogens, not required not required recommended, period required, period
period during which contact of the to be specified in to be specified in
personnel with the host animals should the authorisation the authorisation
be avoided
WASTE MANAGEMENT
53 Inactivation of biological waste and/or required required required required
biological residues using an appropriate
validated method before dumping
54 Inactivation of contaminated material required required required required
(glassware, etc.) using an appropriate and
validated method before reuse or destroying
55 Inactivation of the effluents of sinks and not required not required optional required
showers using an appropriate and validated
method before dumping
4
Suitable footwear (optional)
5
Full change of clothing and shoes before entrance and leaving
Containment requirements 49