Biosafety in the laboratory - VIB

2. Safety equipment
Specifications
Containment levels
G1 G2 G3
25 Autoclave on site in the building in the greenhouse
26 Double-ended autoclave not required not required optional
27 Fumigationroom or immersion bath for not required not required optional
the transfer of living materials
3. Working practices and waste management
Specifications
Containment levels
G1 G2 G3
28 Restricted access required required required
(and control)
29 Notification of the biological risk not required required required
30 Specific equipment not required not required required
31 Protective clothing required required required
(and specific for
the containment zone
32 Decontamination of the protective clothing not required not required required
before it leaves the containment zone
33 Gloves not required optional optional
34 Shoe-coverings or decontamination bath for not required optional optional
the shoes
35 Generation of aerosols minimise minimise prevent
36 Specific measures to prevent the spread of aerosols not required recommended required
37 Mechanical pipetting required required required
38 No drinking, eating or smoking, no use of cosmetics, required required required
no manipulation of contacts, or the stocking of
consumables
39 Register in which all activities are notified required required required
(transport of plant material, inoculation
of GMM’s etc....)
40 Verification of control measures and protective required required required
equipment
41 Notice for the use of decontaminants required required required
42 Instruction of the personnel required required required
43 Written instructions on the biosafety procedures required required required
44 Circulation of animals forbidden forbidden forbidden
45 Measures to control undesired species such as insects, required required required
rodents, arthropods
46 Selfspreading organisms:
- transport in the installation between container: container double
containment zones optional container
- note in the register not required recommended required
- decontamination of containers before transport not required required required
47 Control of contaminated run-off water optional minimise prevent
run-off 14
run-off
WASTE MANAGEMENT
48 Inactivation of biological waste and/or biological required required required
residues using an appropriate and validated method
before dumping
14
If transmission via soil is possible.
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Biosafety in the laboratory