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in Malabing Valley like rampant usage of<br />

fertilizer and pesticide.<br />

Moreover, this was supported by <strong>the</strong><br />

data in Repetition index in which <strong>the</strong> lower <strong>the</strong><br />

value observed <strong>the</strong> higher <strong>the</strong> count of each<br />

species found in <strong>the</strong> area. Kongkong Valley<br />

recorded with a value of 0.56 followed by<br />

Muta Valley with a value of 0.69 and 0.63 for<br />

Malabing Valley. Species richness is greatest<br />

at intermediate frequency and/or intensity of<br />

disturbance. This is because very frequent<br />

disturbance eliminates sensitive species,<br />

w<strong>here</strong>as very infrequent disturbance allows<br />

time for superior competitors to eliminate<br />

species that cannot compete.<br />

In-Vivo Compatibility and Colonization of<br />

VAM Species in Citrus Root System<br />

The portion pointed with <strong>the</strong> larger<br />

arrow shows <strong>the</strong> citrus root penetrated and<br />

invaded by arbuscluar mycorrhizal fungi,<br />

Glomus mosseae in satsuma seedlings. The<br />

part pointed with <strong>the</strong> smaller arrow, labeled MY,<br />

is <strong>the</strong> mycelia of mycorrhiza. The part labeled<br />

EP is <strong>the</strong> epidermal cells of <strong>the</strong> root and <strong>the</strong><br />

part labeled C is <strong>the</strong> cortical cells of <strong>the</strong> root.<br />

Ano<strong>the</strong>r penetration of mycorrhizal<br />

fungi, Glomus mosseae, to <strong>the</strong> cortical cells of<br />

<strong>the</strong> citrus root was observed.<br />

The citrus root of <strong>the</strong> control treated<br />

plants. T<strong>here</strong> are no single fungi that penetrated<br />

<strong>the</strong> root system since <strong>the</strong> soil w<strong>here</strong>in <strong>the</strong><br />

seedlings were planted was sterilized before<br />

<strong>the</strong> experimentation.<br />

Results also show that ample<br />

penetration of <strong>the</strong> mycorrhizal fungi to <strong>the</strong><br />

root system of citrus seedlings inoculated with<br />

fungi. This implies that <strong>the</strong> significant increase<br />

in stem diameter is attributed to <strong>the</strong> presence<br />

and penetration of <strong>the</strong> arbuscular mycorrhizal<br />

fungi, particularly Glomus mosseae, to <strong>the</strong> root<br />

system of <strong>the</strong> plant.<br />

J.I. Yago, et.al<br />

While <strong>the</strong> ponkan seedlings are still<br />

under observation, <strong>the</strong> roots of a seedling<br />

were taken to see and examine <strong>the</strong> penetration<br />

and colonization of <strong>the</strong> fungi. The results of<br />

<strong>the</strong> microscopic observation are shown in <strong>the</strong><br />

figures.<br />

The roots of a plant sample treated<br />

with Gigaspora gigantea was observed under<br />

photomicroscope. In <strong>the</strong> figure, mycelial<br />

penetration of <strong>the</strong> mycorrhizal fungi on <strong>the</strong><br />

epidermal cells of <strong>the</strong> citrus root is clearly seen.<br />

The portion labeled G shows <strong>the</strong> germinated<br />

mycelia of mycorrhizae.<br />

The root system of a ponkan seedling<br />

treated with an unknown fungi species<br />

presumed to be of genus Glomus. Knowing<br />

that <strong>the</strong> roots of <strong>the</strong> satsuma seedlings were<br />

observed two weeks after inoculation of <strong>the</strong><br />

fungi, while <strong>the</strong> ponkan roots were observed<br />

five days after inoculation, <strong>the</strong> results of <strong>the</strong><br />

percent colonization of <strong>the</strong> mycorrhizal fungi<br />

shows that <strong>the</strong>re is better penetration of <strong>the</strong><br />

fungi in <strong>the</strong> younger seedlings than that of <strong>the</strong><br />

older ones. Thus, arbuscular mycorrhizal fungi,<br />

as bioenhancers to citrus species, act better<br />

in <strong>the</strong> earlier stages of <strong>the</strong> development of <strong>the</strong><br />

plant than in later periods.<br />

Plant Tissue Nutrients Concentration of<br />

Citrus Seedlings Inoculated with Indigenous<br />

VAM<br />

Citrus seedlings inoculated with<br />

vesicular-arbuscular mycorrhiza, increased<br />

plant tissue nutrients concentration. Table 4<br />

shows plant tissue phosphorus, nitrogen and<br />

potassium concentration was much higher<br />

in <strong>the</strong> inoculated plants than non inoculated<br />

ones. At yield-maximizing N rates, leaf N<br />

concentrations were 3.35 to 4.50 %; 0.50 to<br />

0.70 % of P and 2.0 to 3.0 % for K which is<br />

generally accepted as critical leaf tissue NPK<br />

concentration range according to (Kallsen,<br />

2003). T he results indicate that tree N status<br />

was adequate at yield-maximizing N rates.<br />

The higher phosphorus concentration<br />

in <strong>the</strong> inoculated plants could be attributed to a<br />

7

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