QCapture PRO 7 User Manual - QImaging

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Adjusting Color Channels Correcting the Background Use the Background Correction command to make adjustments to the background of your image. This tool is used to better distinguish image background from image objects, making it easier to extract the objects during a counting or measurement operation. The Background Correction command can correct uneven background intensities, and compensate for irregularities due to uneven lighting, nonuniform camera response or minor optic imperfections. It might be used to remove evidence of dust on the lens, or to correct for bright spots caused by the light beneath the microscope's stage. Two types of background correction are provided by Q-Capture Pro 7: • Background Subtraction should be used for all images except those of transmitted light experiments which will be used to measure optical density. • Background Correction should be used for optical density applications, as it accounts for the fact that optical density is not a linear function of the gray scale. Q-Capture Pro 7 provides several tools that allow you to adjust the background of your image. They appear in the Background group on the Adjust ribbon: The Options allow you to create a new image from the background image. Extract background image displays the background image during black and white operations. To use the background commands, follow these steps: 1. Open the image that you want to correct. 2. Click the Subtract drop-down arrow . You will see the following dialog: 5-30
Adjusting Color Channels 3. Choose a dark or light background to subtract. The subtracted background will appear in the workspace. You may also use the browse button to choose a saved image to work with; or create a background image from existing points in the active image. 5-31
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USER MANUAL
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Table of Contents License Agreement
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Table of Contents GENERAL 1. You ma
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Table of Contents Table of Contents
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Table of Contents Chapter 7. Using
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Preface Before You Begin Welcome to
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Chapter 1 Installing Q-Capture Pro
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Installing Q-Capture Pro 7 Installi
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Installing Q-Capture Pro 7 The Read
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Chapter 2 Getting Started This chap
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The Q-Capture Pro 7 Application Win
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Chapter 3 Capturing Images This cha
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The Capture Tab 3. Got to Settings,
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The Capture Tab Using Advanced Sett
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The Capture Tab 4. Next, expand the
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The Capture Tab formats available.
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The Capture Tab Saving Your Capture
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The Capture Tab 3. From this panel,
Page 55 and 56: The Capture Tab 3. Draw a line over
Page 57 and 58: The Capture Tab For example, if the
Page 59 and 60: The Capture Tab If there is a featu
Page 61 and 62: The Capture Tab Applying a Calibrat
Page 63 and 64: The Capture Tab TIP: You may need t
Page 65 and 66: Chapter 4 Viewing Intensity Informa
Page 67 and 68: Viewing a Image Histogram Right-cli
Page 69 and 70: Viewing a Line Profile 3. When you
Page 71 and 72: Viewing a Line Profile Polygon/Poly
Page 73 and 74: Viewing a Line Profile The Options
Page 75 and 76: Using the Saturation Warning The Re
Page 77 and 78: Chapter 5 Working with Images This
Page 79 and 80: Opening an Image Opening an Image T
Page 81 and 82: Opening an Image Opening an Recent
Page 83 and 84: Opening an Image Using Quick Save I
Page 85 and 86: Zooming Images Zooming Images The z
Page 87 and 88: Panning Images 1. Position the curs
Page 89 and 90: Enhancing an Image Enhancing an Ima
Page 91 and 92: Enhancing an Image Rotating an Imag
Page 93 and 94: Enhancing an Image Using the Canvas
Page 95 and 96: Using Color Group Commands Using Co
Page 97 and 98: Using Color Group Commands Click Ap
Page 99 and 100: Using Color Group Commands Detailed
Page 101 and 102: Adjusting Color Channels Adjusting
Page 103 and 104: Adjusting Color Channels Changing t
Page 105: Adjusting Color Channels Gamma: Adj
Page 109 and 110: Adjusting Color Channels 4. Select
Page 111 and 112: Image Sequences Use active range ap
Page 113 and 114: Chapter 6 Using Annotations Annotat
Page 115 and 116: Creating an Annotation Creating a G
Page 117 and 118: Creating an Annotation Auto-Trace.
Page 119 and 120: Arranging Annotations The specified
Page 121 and 122: Arranging Annotations 3. Click on t
Page 123 and 124: Setting the Appearance of Annotatio
Page 125 and 126: Setting the Appearance of Annotatio
Page 127 and 128: Chapter 7 Using ROIs You can define
Page 129 and 130: Defining an ROI Defining an ROI Bef
Page 131 and 132: Delete Selected/Delete All: Use the
Page 133 and 134: Arranging ROIs If the cursor is pos
Page 135 and 136: Setting the Appearance of ROIs Sett
Page 137 and 138: Setting the Appearance of ROIs Labe
Page 139 and 140: Chapter 8 Measuring Objects in Imag
Page 141 and 142: Making Manual Measurements Making M
Page 143 and 144: Making Manual Measurements You can
Page 145 and 146: Manual Tagging 5. Save your data to
Page 147 and 148: Manual Tagging 7. Use the Outlines
Page 149 and 150: Viewing Measurement Data The Data T
Page 151 and 152: Chapter 9 Customizing the User Inte
Page 153 and 154: Using the Quick Access Toolbar To A
Page 155 and 156: Using the Quick Access Toolbar The
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Customizing the Image Strip If you
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Customizing the Image Strip Viewing
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Customizing the Image Strip Activat
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Appendix A - File Format Specificat
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Index * *.AVI, 10‐1 *.BMP, 10‐1
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Index G Gain, 3‐6 Gamma, 3‐6 ga
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Index setting the appearance of, 7
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QCapture PRO 7 User Manual - QImaging

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