Lovis 2000 M_ME - Reference Manual.pdf

Reference ManualLovis 2000 M/MEInstrument Software Version: 2.30 - Build 245andInstrument Software Version: 2.32 - Build 32

Reference ManualLovis 2000 M/MEInstrument Software Version: 2.30 - Build 245andInstrument Software Version: 2.32 - Build 32

Anton Paar GmbH assumes no liability for technical or printing errors or omissions in this document.Nor is any liability assumed for damages resulting from information contained in the document.Anton Paar GmbH reserves the right to content changes. This also extends to changes to deliveryvolumes or any features of delivered parts.All rights reserved (including translation). This document, or any part of it, may not be reproduced,changed, copied, or distributed by means of electronic systems in any form (print, photocopy, microfilmor any other process) without prior written permission by Anton Paar GmbH.Trademarks, registered trademarks, trade names, etc. may be used in this manual without being markedas such. They are the property of their respective owner.Published by Anton PaarPrinted: Anton Paar, AustriaCopyright © 2012 Anton Paar GmbH, Graz, AustriaAddress:Anton Paar GmbHAnton-Paar-Str. 20A-8054 Graz / Austria – EuropeTel: +43 316 257-0Fax: +43 316 257-257Date: November 2012E-Mail: info@anton-paar.comWeb: www.anton-paar.comDocument number C72IB002EN-E

Contents1 About the Reference Manual ........................................................................................................ 122 Safety Instructions......................................................................................................................... 152.1 General Safety Instructions ................................................................................................... 152.2 Special Safety Instructions .................................................................................................... 172.3 Warning Signs on the Instrument .......................................................................................... 18SECTION I: Lovis 2000 M/ME Reference........................................................................................... 193 Lovis 2000 M/ME - An Overview ................................................................................................... 203.1 Intended Use of the Machinery.............................................................................................. 213.1.1 Measurement results without Density Values......................................................... 213.1.2 Density Values for Viscosity Calculation................................................................. 213.1.3 Instrument Firmware Versions................................................................................ 223.2 Lovis Measuring Principle...................................................................................................... 233.3 Available Capillaries and Filling Methods .............................................................................. 244 Checking the Supplied Parts ........................................................................................................ 254.1 Supplied Parts Lovis 2000 M ................................................................................................. 254.2 Options .................................................................................................................................. 265 Functional Components................................................................................................................ 275.1 Lovis 2000 M ......................................................................................................................... 275.1.1 Lovis 2000 M Front and Left Side View.................................................................. 275.1.2 Lovis 2000 M Right Side View ............................................................................... 285.1.3 Lovis 2000 M Rear View ........................................................................................ 285.2 Module Housing Lovis ME..................................................................................................... 295.2.1 Module Housing Front View .................................................................................. 295.2.2 Module Housing Right Side View ........................................................................... 295.2.3 Module Housing Rear View.................................................................................... 305.3 Operating Elements of the Main Screen................................................................................ 305.4 Operating Elements of the Menu Screen............................................................................... 336 Preparing the Instrument .............................................................................................................. 346.1 Installation Requirements ...................................................................................................... 346.2 Mounting Lovis 2000 M.......................................................................................................... 356.3 Switching Lovis 2000 M On/Off ............................................................................................. 366.4 Mounting Lovis 2000 ME Module in the Master Instrument or in the Module Housing Lovis ME..................................................................................................... 366.5 Adjusting the Foot of the Module Housing Lovis ME............................................................. 396.6 Connecting the Cables to Module Housing Lovis ME............................................................ 406.7 Switching Lovis 2000 ME On/Off ........................................................................................... 41C72IB002EN-E 5

7 Settings and Methods.................................................................................................................... 427.1 Measuring System Settings................................................................................................... 427.1.1 Capillary Administration.......................................................................................... 427.1.2 Capillary Detection ................................................................................................. 437.1.3 Capillary Filling Mode ............................................................................................. 447.1.4 Polymer Solvents Administration............................................................................ 447.2 Lovis Measuring Methods Settings........................................................................................ 457.2.1 Factory Predefined Methods .................................................................................. 457.2.2 Creating, Editing and Deleting Measuring Methods ............................................... 487.2.3 Measurement Mode Settings ................................................................................. 487.2.4 Formula Parameters............................................................................................... 507.2.5 Lovis Module Settings ............................................................................................ 517.2.5.1 Temperature Settings.............................................................................. 517.2.5.2 Measuring Settings ................................................................................. 517.2.5.3 Advanced Settings .................................................................................. 527.2.5.4 Density Settings ...................................................................................... 537.2.5.5 Beer/Wort Settings .................................................................................. 547.2.5.6 Polymer Settings..................................................................................... 547.2.5.7 Molar Mass Calculation Settings............................................................. 568 Checks and Adjustments for Lovis 2000 M/ME .......................................................................... 578.1 Definitions.............................................................................................................................. 578.2 Checks................................................................................................................................... 588.2.1 Editing the Check Settings for Lovis 2000 M/ME ................................................... 588.2.2 Performing Checks................................................................................................. 598.2.3 Viewing, Printing or Exporting Current Check Data................................................ 608.3 Performing a Level Adjustment.............................................................................................. 618.4 Adjusting Lovis 2000 M/ME Capillaries ................................................................................. 628.5 Temperature Adjustment........................................................................................................ 648.5.1 Performing a Manual Temperature Adjustment...................................................... 648.5.2 Performing a Temperature Adjustment with MKT 50.............................................. 668.5.3 Resetting the Temperature Factory Adjustment Data ............................................ 688.6 Viewing, Printing or Exporting Adjustment Data .................................................................... 689 Filling the Lovis 2000 M/ME .......................................................................................................... 699.1 Selecting a Capillary and a Ball.............................................................................................699.2 Filling a Standard Capillary Outside the Capillary Block........................................................ 699.3 Filling a Short Capillary.......................................................................................................... 739.4 Inserting a Filled Capillary ..................................................................................................... 779.5 Mounting a Capillary for Flow-Through Filling ....................................................................... 789.6 Hose Connections Flow-through Filling................................................................................. 809.7 Filling the Flow-Through Assembly Manually with a Syringe................................................. 8310 Performing a Measurement........................................................................................................... 8510.1 Preparing a Measurement .....................................................................................................856 C72IB002EN-E

16.2 Options ................................................................................................................................ 12216.2.1 Drip Plate.............................................................................................................. 12216.2.2 Waste Bottle ......................................................................................................... 12216.2.3 Chemical Resistant Hose Sets............................................................................. 12316.2.4 Low Volume Options ............................................................................................ 12416.2.5 Stands and Waste Containers ............................................................................. 12517 Preparing Module(s) and Master Instrument............................................................................. 12617.1 Steps to Perform the Hardware Setup................................................................................. 12617.2 Preparing the Safety Cover ................................................................................................. 12717.3 Installing the Drip Plate........................................................................................................ 13017.4 Optional Waste Vessels....................................................................................................... 13217.4.1 Waste Container 8 L HDPE.................................................................................. 13217.4.2 Waste Bottle 1 L Glass......................................................................................... 13417.4.3 Replacing the Sealing Gasket .............................................................................. 13417.5 CAN Power Supply.............................................................................................................. 13517.6 General Information for Installation...................................................................................... 13618 Lovis 2000 M + Xsample.............................................................................................................. 13718.1 Lovis 2000 M + Xsample 352/452 ....................................................................................... 13718.1.1 Preparing Master Instrument and Modules .......................................................... 13718.1.2 Connecting the Hoses ......................................................................................... 13818.1.3 Low Volume Setup ............................................................................................... 14118.2 Lovis 2000 M + Xsample 52 ................................................................................................ 14218.2.1 Preparing Master Instrument and Modules .......................................................... 14218.2.2 Connecting the Hoses ......................................................................................... 14318.3 Lovis 2000 M + Xsample 122 .............................................................................................. 14718.3.1 Preparing Master Instrument and Modules .......................................................... 14718.3.2 Connecting the Standard Hoses........................................................................... 14818.3.2.1 Filling by Pressure .............................................................................. 14918.3.2.2 Filling by Suction ............................................................................... 15318.3.3 Connecting Chemically Resistant Hoses ............................................................. 15718.3.3.1 Filling by Pressure................................................................................. 15718.3.3.2 Filling by Suction................................................................................... 16018.4 Lovis 2000 M + Xsample 22 ................................................................................................ 16318.4.1 Preparing Master Instrument and Modules .......................................................... 16318.4.2 Connecting the Standard Hoses........................................................................... 16318.4.2.1 Filling by Pressure ................................................................................ 16418.4.2.2 Filling by Suction .................................................................................. 16818.4.3 Connecting Chemically Resistant Hoses ............................................................. 17118.4.3.1 Filling by Pressure................................................................................. 17118.4.3.2 Filling by Suction................................................................................... 17319 Lovis 2000 ME + DMA M / DSA M + Xsample ............................................................................ 17619.1 Lovis 2000 ME + DMA M + Xsample 352/452..................................................................... 1768 C72IB002EN-E

19.1.1 Preparing Master Instrument and Modules .......................................................... 17619.1.2 Connecting the Hoses .......................................................................................... 17719.1.3 Low Volume Setup ............................................................................................... 18119.2 Lovis 2000 ME + DMA M + Xsample 52.............................................................................. 18219.2.1 Preparing Master Instrument and Modules .......................................................... 18219.2.2 Connecting the Hoses .......................................................................................... 18219.3 Lovis 2000 ME + DMA M / DSA M + Xsample 122 ............................................................. 18619.3.1 Preparing Master Instrument and Modules .......................................................... 18619.3.2 Connecting the Standard Hoses........................................................................... 18719.3.2.1 Filling by Pressure ................................................................................ 18819.3.2.2 Filling by Suction .................................................................................. 19319.3.3 Connecting Chemically Resistant Hoses ............................................................. 19719.3.3.1 Filling by Pressure................................................................................. 19819.3.3.2 Filling by Suction................................................................................... 20119.4 Lovis 2000 ME + DMA M /DSA M + Xsample 22 ................................................................ 20419.4.1 Preparing Master Instrument and Modules .......................................................... 20419.4.2 Connecting the Standard Hoses........................................................................... 20419.4.2.1 Filling by Pressure................................................................................. 20519.4.2.2 Filling by Suction .................................................................................. 20919.4.3 Connecting Chemically Resistant Hoses ............................................................. 21319.4.3.1 Filling by Pressure................................................................................. 21319.4.3.2 Filling by Suction................................................................................... 21520 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample.......................... 21820.1 Lovis 2000 ME + DMA M + Alcolyzer ME + HazeQC ME + pH ME + Xsample 122/22 - Vertical Setup ......................................................................................... 21920.1.1 Preparing Master Instrument and Modules .......................................................... 22020.1.2 Connecting the Hoses - Vertical Setup ................................................................ 22520.2 Lovis 2000 ME + DMA M + Alcolyzer ME + HazeQC ME + pH ME + Xsample 122/22 - Horizontal Setup ..................................................................................... 23220.2.1 Preparing Master Instrument and Modules .......................................................... 23220.2.2 Connecting the Hoses .......................................................................................... 23620.3 Lovis 2000 ME + DMA M + Alcolyzer ME or HazeQC ME + pH ME + Xsample 122/22................................................................................................................... 24120.3.1 Preparing Master Instrument and Modules .......................................................... 24120.3.2 Connecting the Hoses .......................................................................................... 24220.4 Lovis 2000 ME + DMA M + Alcolyzer ME or HazeQC ME + Xsample 122/22 .................... 24620.4.1 Preparing Master Instrument and Modules .......................................................... 24620.4.2 Connecting the Hoses .......................................................................................... 24721 Assemblies with pH ME............................................................................................................... 25121.1 DMA M/DSA M + Lovis 2000 ME + pH ME - Manually Filled by Syringe ............................ 25121.1.1 Preparing Master Instrument and Modules .......................................................... 25121.1.2 Connecting the Hoses ....................................................................................... 252C72IB002EN-E 9

21.2 DMA M/DSA M + Lovis 2000 ME + pH ME + Xsample 122/22 ........................................... 25521.2.1 Preparing Master Instrument and Modules .......................................................... 25521.2.2 Connecting the Hoses .......................................................................................... 25722 Assemblies with Abbemat .......................................................................................................... 26322.1 Lovis 2000 M + Abbemat Manual Flow-Through Filling by Syringe .................................... 26422.1.1 Preparing Master Instrument and Modules .......................................................... 26422.1.2 Connecting the Hoses .......................................................................................... 26622.2 Lovis 2000 ME + DMA M/DSA M + Abbemat Manually Filled by Syringe........................... 26822.2.1 Preparing Master Instrument and Modules .......................................................... 26822.2.2 Connecting the Hoses .......................................................................................... 27122.3 Lovis 2000 ME + DMA M/DSA M + Abbemat + Xsample 122/22........................................ 27422.3.1 Preparing Master Instrument and Modules .......................................................... 27422.3.2 Connecting the Hoses .......................................................................................... 27622.4 Lovis 2000 ME + DMA M/DSA M + HazeQC ME + Abbemat + Xsample 122/22................ 27922.4.1 Preparing Master Instrument and Modules .......................................................... 28022.4.2 Connecting the Hoses .......................................................................................... 28322.5 Lovis 2000 ME + DMA M/DSA M + Abbemat + pH ME + Xsample 122/22......................... 28822.5.1 Preparing Master Instrument and Modules .......................................................... 28822.5.2 Connecting the Hoses ......................................................................................... 29122.6 Lovis 2000 ME + DMA M/DSA M + HazeQC ME + Abbemat + pH ME + Xsample 122/22................................................................................................................... 29622.6.1 Preparing Master Instrument and Modules .......................................................... 29622.6.2 Connecting the Hoses ......................................................................................... 29822.7 Lovis 2000 ME + DMA M + Abbemat + Xsample 352/452 .................................................. 30222.7.1 Preparing Master Instrument and Modules .......................................................... 30222.7.2 Connecting the Hoses .......................................................................................... 30623 Compressed Air - Air Preparation Sets...................................................................................... 31023.1 Air Preparation Set Without Dryer ....................................................................................... 31023.1.1 Parts of the Air Preparation Set .......................................................................... 31023.1.2 Mounting the Air Preparation Set ..........................................................................31123.1.3 Connecting the Air Hoses......................................................................................31123.1.4 Setting the Pressure on the Air Preparation Unit ................................................. 31223.2 Air Preparation Set for Dew Point -40 °C............................................................................. 31323.2.1 Parts of the Air Preparation Set ........................................................................... 31323.2.2 Mounting the Air Preparation Set ......................................................................... 31423.2.3 Connecting the Air Hoses..................................................................................... 31424 Putting a Measuring Assembly into Operation......................................................................... 31625 Adjusting Lovis 2000 ME with Xsample..................................................................................... 31925.1 Level Adjustment with Xsample........................................................................................... 31925.2 Capillary Adjustment with Xsample ..................................................................................... 32026 Customer Maintenance Assemblies .......................................................................................... 32326.1 Inspection of Hoses and Hose Connectors ......................................................................... 32410 C72IB002EN-E

26.2 Leak Test of all Connected Hoses ....................................................................................... 32426.2.1 Measuring Assemblies with Xsample 352/452..................................................... 32426.2.2 Measuring Assemblies with Xsample 52.............................................................. 32526.2.3 Measuring Assemblies with Xsample 122/22....................................................... 326Appendix A: Technical Data ......................................................................................................... 328A.1 Measuring Performance .................................................................................... 328A.2 Lovis 2000 M/ME Capillary Measuring Ranges ................................................ 329A.3 Recommended Viscosity Standard Liquids ....................................................... 329A.4 Minimum Required Sample Volume Assemblies............................................... 330A.5 General Technical Data .................................................................................... 332Appendix B: Wetted Parts and Chemical Compatibility............................................................. 334B.1 Wetted Parts...................................................................................................... 334B.2 Parts Possibly in Contact with Sample Liquid ................................................... 337B.3 Improving the Chemical Resistance.................................................................. 338B.4 Overview Chemical Compatibility ..................................................................... 340Appendix C: Menu Tree................................................................................................................. 342Appendix D: Lovis Parameters .................................................................................................... 347D.1 Output Data Groups ......................................................................................... 347D.2 Lovis Quick Settings Parameters ...................................................................... 349Appendix E: Measuring under Special Conditions .................................................................... 352E.1 Measuring at High Humidity/Low Temperature Conditions ............................... 352E.2 Measuring in Harsh Environments .................................................................... 353Appendix F: Trouble Shooting..................................................................................................... 355F.1 Error Messages and Warnings.......................................................................... 355F.2 List of error messages....................................................................................... 358F.3 Problems and Solutions ................................................................................... 360Appendix G: Instrument Firmware Versions, Instruction Manuals........................................... 362Index................................................................................................................................................... 366List of Figures ................................................................................................................................... 371C72IB002EN-E 11

1 About the Reference Manual1 About the Reference ManualThis reference manual is split into two sections and an Appendix:SECTION I - Lovis Reference InformationThis section contains all chapters of the Lovis 2000 M/ME Original Instructionplus additional information to many chapters of the printed Instruction Manualsuch as• Checks• Specific measuring settings• Editing advanced method settings• Polymer application settings (for more details refer to the PolymerApplication Report, Doc. No. C72IA023EN, supplied on the USB flash drive)• Service utilitiesand more.It describes also operating Lovis 2000 ME inside a DMA M/DSA M model.Additionally read and adhere to all instructions given in a DMA M/DSA Minstruction manual.SECTION II - Flow-Through SystemsThis section informs you about the hardware setup and the operation ofmeasuring assemblies consisting of Lovis 2000 M/ME with several Xsamplesample changer models, DMA M/DSA M density/sound velocity meters and othermodules.AppendixThe Appendix with helpful information about• Measurement under special conditions• Lovis parameters• Chemical compatibility of wetted parts• Troubleshooting• Full menu structureand more completes the reference manual.Pay special attention to the safety instructions and warnings in this manual andon the product. It is a part of the product, included on the USB flash drive suppliedwith Lovis 2000 M/ME. It is available only as a pdf file in English language.Keep this documentation for the complete working life of the product and makesure it is easily accessible to all people involved with the product. If you receiveany additions or revisions to this reference manual from Anton Paar GmbH, thesemust be treated as part of the reference manual.12 C72IB002EN-E

1 About the Reference ManualConventions for safety messagesThe following conventions for safety messages are used in this instructionmanual:DANGERDanger indicates a hazardous situation which, if not avoided, will result indeath or serious injury.WARNINGWarning indicates a hazardous situation which, if not avoided, could result indeath or serious injury.CAUTIONCaution indicates a hazardous situation which, if not avoided, could result inminor or moderate injury.HazardThis symbol calls attention to the fact that other hazards can occur, e.g. duringsample handling. Follow the instructions.Wear protective glovesWear protective gloves when handling the Lovis 2000 M/ME.Wear safety gogglesWear safety goggles when handling the Lovis 2000 M/ME.Use protective clothingUse protective clothing when handling the Lovis 2000 M/ME.NOTICENotice indicates a situation which, if not avoided, could result in damage toproperty.TIPTip gives extra information about the situation at hand.C72IB002EN-E 13

1 About the Reference ManualTypographical conventionsThe following typographical conventions are used in this instruction manual:Convention"Menu Level 1 > Menu Level 2"DescriptionThe names of keys and buttons arewritten inside angle brackets.Menu paths are written in bold, insidestraight quotation marks. The menulevels are connected using a closingangle bracket.14 C72IB002EN-E

2 Safety Instructions2 Safety Instructions• Read this reference manual before using Lovis 2000 M/ME.• Follow all hints and instructions contained in this reference manual to ensurethe correct use and safe functioning of Lovis 2000 M/ME.2.1 General Safety InstructionsLiability• This reference manual does not claim to address all safety issues associatedwith the use of the instrument and samples. It is your responsibility toestablish health and safety practices and determine the applicability ofregulatory limitations.• Anton Paar GmbH only warrants the proper functioning of Lovis 2000 M/MEif no adjustments have been made to the mechanics, electronics, modulefirmware and instrument firmware.• Only use Lovis 2000 M/ME for the purpose described in this instructionmanual. Anton Paar GmbH is not liable for damages caused by incorrect useof Lovis 2000 M/ME.Installation and use• Lovis 2000 M/ME is not an explosion-proof instrument and therefore mustnot be operated in areas with risk of explosion.• The installation procedure should only be carried out by authorizedpersonnel who are familiar with the installation instructions.• Do not use any accessories or spare parts other than those supplied orapproved by Anton Paar GmbH (see Chapter 4).• Make sure all operators are trained to use the instrument safely and correctlybefore starting any applicable operations.• In case of damage or malfunction, do not continue operating Lovis 2000 M/ME. Do not operate the instrument under conditions whichcould result in damage to goods and/or injuries and loss of life.• Check Lovis 2000 M/ME for chemical resistance to the samples andcleaning liquids.C72IB002EN-E 15

2 Safety InstructionsMaintenance and service• The results delivered by Lovis 2000 M/ME not only depend on the correctfunctioning of the instrument, but also on various other factors. We thereforerecommend you have the results checked (e.g. plausibility tested) by skilledpersonnel before consequential actions are taken based on the results.• Service and repair procedures may only be carried out by authorizedpersonnel or by Anton Paar GmbH.Disposal• Concerning the disposal of Lovis 2000 M/ME, observe the legalrequirements in your country.Returns• For repairs contact your Anton Paar representative. Remove sample andsolvent liquids and clean the Lovis 2000 M/ME unit before sending it to yourAnton Paar representative.Precautions for highly inflammable samples and cleaning agents• Observe and adhere to your national safety regulations for handling themeasured samples and used cleaning liquids (e.g. use of safety goggles,gloves, respiratory protection etc.).• Keep any sources of ignition, like sparks and open flames, at a safe distancefrom Lovis 2000 M/ME.• Place Lovis 2000 M/ME on a laboratory bench with a non-flammablesurface, preferably made of bricks, ceramics or stoneware.• Only store the minimum required amount of sample, cleaning agents andother inflammable materials near the Lovis 2000 M/ME.• Do not spill sample/cleaning agents or leave their containers uncovered.Immediately remove spilled sample/cleaning agents.• Make sure that the setup location is sufficiently ventilated. The environmentof Lovis 2000 M/ME must be kept free of inflammable gases and vapors.• Connect Lovis 2000 M or the DMA M/DSA M model containingLovis 2000 ME to the mains via a safety switch located at a safe distancefrom the instrument. In an emergency, turn off the power using this switchinstead of the power switch on Lovis 2000 M or the DMA M/DSA M masterinstrument.• Supply a fire extinguisher.• Ensure the sufficient supervision of Lovis 2000 M/ME during operation.16 C72IB002EN-E

2 Safety Instructions2.2 Special Safety Instructions• Do not use Lovis 2000 M/ME respectively the measuring assembly withincluded Lovis 2000 M/ME to measure fluids of the IIC group according toEN 60079-14, such as e.g. carbon disulfide, hydrogen or acetylene.• Do not use Lovis 2000 M/ME respectively the measuring assembly withincluded Lovis 2000 M/ME to measure fluids, such as diethyl ether, abovetemperature class T4 according to ATEX Directive 94/9/EC.• If liquid was spilled over the instrument, disconnect the instrument from themains. Clean and dry the housing of the instrument. If you have a suspicionthat liquid got into the instrument, have the instrument cleaned and checkedfor electrical safety by a service technician.• Ensure that spilled liquids can not get into plug connections or venting slotsof electrical equipment.• If placing a module on top of a master instrument (Lovis 2000 M or DMA M/DSA M) with built-in Xsample model use a Drip Plate (Mat. No. 99384) toprotect the master instrument against liquid entry and to ensure electricalsafety in case of leakage. If combining a master instrument with severalmodules, it is recommended to use the Stand Measuring Assembly (Mat.No. 105451) instead of the Drip Plate. See also SECTION II.12Fig. 2 - 1 Drip Plate (1)and Stand Measuring Assembly (2)• Make sure that the capillary was removed from the Lovis capillary blockbefore you move or lift Lovis 2000 M/ME.• To move or lift Lovis 2000 M, place one hand at the back and grip the ledgeat the top. Place the other hand at the front, under the display. There is ahollow for your fingers.• To move or lift Lovis 2000 ME in the Module Housing, place one hand at theback and grip the base plate. Place the other hand at the front and grip thebase plate. To move or lift Lovis 2000 ME in a DMA M/DSA M, refer to thesafety instructions in the respective instruction manual.• When carrying the instrument or parts of the measuring assembly, keepthem close to your body.C72IB002EN-E 17

2 Safety Instructions2.3 Warning Signs on the InstrumentHazardThis symbol calls attention to the fact that other hazards can occur, e.g. duringsample handling. Follow the instructions.Warning symbol "Hazard" on the Lovis capillary block:Fig. 2 - 2Symbol "Hazard" on the Lovis capillary block• Handling caustic liquids, acids, or biologically hazardous, easily inflammableliquids etc. involves a serious health risk for the operator. Use appropriateprotective equipment (protective gloves, safety goggles, protective clothing,fume hood...).• When switching on the Lovis 2000 M/ME instrument, the Lovis capillaryblock moves into the physical end position (initialization). The moving blockcould possibly squeeze your fingers. Touching the moving block might leadto clamping or displacement of the zero position. Do not touch the movingLovis capillary block or bring parts of your body or objects near it.• Switch off the instrument before moving the Lovis capillary block by hand!• Risk of burns at measuring temperatures above 60 °C at the insert openingsof the Lovis capillary block and on the capillary. Do not remove the hotcapillary and do not touch the insert openings of the Lovis capillary blockwhen hot. Either use adequate protective gloves or cool down theLovis 2000 M/ME to a temperature significantly below 60 °C before touchingthese parts with bare hands.• Due to thermal expansion of the sample at elevated measuringtemperatures, the plugs which seal the capillary after filling manually outsidethe Lovis capillary block may pop off. Risk of burns or injury caused by hot,toxic or aggressive liquid splashes. Risk of injury caused by the popping offplug. To prevent that, always attach the closed screw cap front side and backside for this filling method. Refer to the instructions in Chapter 9.1.18 C72IB002EN-E

SECTION I : Lovis 2000 M/ME ReferenceThis section contains all chapters of the Lovis 2000 M/ME Original Instruction.Some chapters also give additional information to the Lovis 2000 M/ME OriginalInstruction.C72IB002EN-E 19

3 Lovis 2000 M/ME - An Overview SECTION I : Lovis 2000 M/ME Reference3 Lovis 2000 M/ME - An OverviewLovis 2000 M/ME is a microviscometer based on the Rolling Ball Principleexplained in Chapter 3.2. It is designed for measuring dynamic, kinematic,relative and intrinsic viscosity of liquids especially in the low viscosity range.Fig. 3 - 1Lovis 2000 M and Lovis 2000 ME with DMA M and XsampleIt is a microviscometer due to the small sample volume required (< 1mL). Thesample is filled into a glass capillary which is introduced into a temperaturecontrolled capillary block. This block can be inclined at a variable predefinedangle. Varying the inclination angle permits to extend the measuring range of acapillary and to submit a sample to different shear rates.The Lovis 2000 M is an instrument for stand-alone operation.The Lovis 2000 ME is a modular instrument for inserting into• DMA 4100/4500/5000 M density meter or• DSA 5000 M sound velocity meter.Optionally, the Lovis 2000 M/ME can also be connected to an automatic samplechanger (Xsample) and to other Anton Paar measuring modules.For combining Lovis 2000 ME with a DMA M/DSA M plus an Xsample and/oradditional modules, refer to SECTION II : Flow-Through Systems.Data management and safetyThe Lovis 2000 M/ME offers up-to-date data management and safety features tomake your work easier and help you comply with your quality managementregulations:• Print out reports.• Export your data in the format of your choice (MS Excel, text or PDF).• Choose between the interfaces: 4 x USB, Ethernet, RS-232 and CAN BUS.• Use the powerful audit trail function with tamper-proof data export(checksum).• Rely on full compliance with GLP/GMP and 21 CFR part 11.20 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference3 Lovis 2000 M/ME - An Overview3.1 Intended Use of the MachineryLovis 2000 M/ME is intended for measuring the rolling times of a ball in liquidsamples and calculating the samples’ viscosities from the obtained times. It isdesigned for use under the ambient conditions specified in Appendix A.5.• Lovis 2000 M/ME is ideally suited for samples in the low viscosity range, e.g.pharmaceutical liquids, polymer solutions, beer and wort or ink for ink-jetprinters.• The variable inclination angle predestines Lovis 2000 M/ME for testingpolymer solutions requiring extrapolation to zero shear rate.• Do not fill a sample if you do not know which cleaning liquid removes thissample. Make sure the wetted parts of Lovis 2000 M/ME are chemicallyresistant to any tested sample or cleaning liquid. Do not fill and test liquidsthat attack the instrument’s materials, e.g. hydrofluoric acid (attacks glass).3.1.1 Measurement results without Density ValuesThe liquid’s relative viscosity and intrinsic viscosity and further polymersolution parameters can be calculated from the rolling times alone. It is notnecessary to adjust the capillary plus the ball. The sample density must not beknown.These viscosities are relative values. In their equations the density values canceleach other out.3.1.2 Density Values for Viscosity CalculationTo calculate a dynamic viscosity or kinematic viscosity value from themeasured rolling times the sample’s density must be known.In addition, the capillary plus a ball needs to be adjusted with a substance ofknown viscosity and density. The density of the ball must also be known.A ball’s density is given on the label of the ball vessel, along with the ID numberof the ball batch. There are two possibilities how to obtain the sample density:Manual Input of Sample DensityThe sample density can be entered manually before starting a test withLovis 2000 M/ME. Either the value is already known or it has been testedbeforehand with a density meter.Simultaneous Density Measurement with DMA M/DSA MLovis 2000 ME is combined with a density meter or sound velocity meter of AntonPaar GmbH. Both instruments are connected with hoses and simultaneously filledwith the same sample. The density is measured at the same time as the rollingtime.C72IB002EN-E 21

3 Lovis 2000 M/ME - An Overview SECTION I : Lovis 2000 M/ME Reference3.1.3 Instrument Firmware VersionsThis reference manual applies to instrument firmware versions 2.30 and 2.32.The two versions offer different features. To show the firmware version of yourinstrument tap "Menu > Service > System Information".Instrument FirmwareVersionInstrument Firmware 2.30Build 245 and higherInstrument Firmware 2.32Supports• The use of Lovis 2000 M/MEin combination with thefollowing Anton Paarinstruments and samplehandling units:-DMA M- DSA 5000 M- Abbemat 300/350/500/550-Alcolyzer ME- Haze QC ME-pH ME- Xsample 22/122-Xsample 52- Xsample 352/452• Measuring methods andevaluation for polymerapplications. Measurementmodes "Polymer (P)" and"Zero Shear Scan (ZSS)".• In the data memory: Graphicdisplay of measurementresults for scan tests (Temp.and Temp. Table Scan, FixedAngle Scan, Time Scan andZero Shear Scan).• Selectable angle andVariation Coefficient for LevelAdjustment• The use of short capillariesfor low sample volume.• The use of Lovis 2000 M/MEin combination with thefollowing Anton Paarinstruments and samplehandling units:-DMA M- Xsample 22/122Does Not Support• Measuring methods andevaluation for polymerapplications. Measurementmodes "Polymer (P)" and"Zero Shear Scan (ZSS)".• Selectable angle andVariation Coefficient forLevel Adjustment• In the data memory: Graphicdisplay of measurementresults for scan tests (Temp.and Temp. Table Scan,Fixed Angle Scan, TimeScan and Zero Shear Scan).• The use of short capillariesfor low sample volume.• The use of Lovis 2000 M/MEin combination with thefollowing Anton Paarinstruments and samplehandling units:- DSA 5000 M- Abbemat 300/350/500/550-Alcolyzer ME- Haze QC ME-pH ME-Xsample 52- Xsample 352/45222 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference3 Lovis 2000 M/ME - An Overview3.2 Lovis Measuring PrincipleThe Rolling Ball Principle goes back to the“Falling-Ball Viscometer” designed by FritzHöppler in 1933 (see also DIN 53015, and laterISO 12058).A ball rolls through a closed, liquid-filled capillary,which is inclined at a defined angle.Three inductive sensors determine the ball'srolling time between defined marks. Thesample's viscosity is directly proportional to therolling time (the greater the viscosity the longerthe rolling time). The microviscometer registersthe rolling time.The inductive sensors allow the measurement ofopaque liquids.F G driving gravity forceF V retarding viscous forceF B retarding buoyancy forceFig. 3 - 2 Lovis measuring principleBy using these three sensors the total measuringdistance of Lovis 2000 M/ME is divided in a shortfirst part and a longer second part. When theinstrument starts measuring, the ball first passesthe short distance; the obtained time showswhether the sample is highly viscous or not.With highly viscous samples, time can be saved by measuring only the rolling timefor the short distance. The capillary block is turned around as soon as the ball haspassed sensor No. 2. This applies also to short capillaries for low sample volume.C72IB002EN-E 23

3 Lovis 2000 M/ME - An Overview SECTION I : Lovis 2000 M/ME Reference3.3 Available Capillaries and Filling MethodsThis chapter gives an overview of the capillaries and the filling methods. Finddetailed information in the Lovis 2000 M/ME Product Description List (providedon the supplied USB flash drive).Standard Capillary for Manual Filling Outside the Capillary Block3 2 19 1048Fig. 3 - 3Standard capillary and accessories for manual fillingStandard Capillary for Flow-Through Filling15 511 128Fig. 3 - 4Standard capillary and accessories for flow-through fillingShort Capillary for Low Sample Volume (Manual Filling Outside the Capillary Block)6 769 2108Fig. 3 - 5Short capillary and accessories for low sample volume1 ... Capillary2 ... Filling Adapter Luer Female3 ... Luer Plug4 ... Capillary Plug (PVDF)5 ... Filling Adapter UNF6 ... Short Capillary7 ... Capillary Plug Hollow (PCTFE)8 ... Ball9 ... Screw Cap Front Side Closed10 ... Screw Cap Back Side Closed11 ... Screw Cap Front Side Open12 ... Screw Cap Back Side Open24 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference4 Checking the Supplied Parts4 Checking the Supplied PartsLovis 2000 M/ME was tested and packed carefully before shipment. However,damage may occur during transport.1. Keep the packaging material (box, foam piece, transport protection) forpossible returns and further questions from the transport and insurancecompany.2. Check the delivery for completion by comparing the supplied parts to thoselisted in the corresponding tables. The capillary/capillaries, balls and fillingaccessories supplied with Lovis 2000 M/ME depend on which parts wereactually ordered. Compare the delivered items to the delivery list. Finddetailed information on all capillary accessories and optional equipment inthe Lovis 2000 M/ME Product Description List (provided on USB flash drive).For parts supplied with DMA M series, DSA 5000 M and other modules seethe respective instruction manuals and delivery lists.3. If a part is missing, contact your Anton Paar representative.4. If a part is damaged, contact the transport company and your Anton Paarrepresentative.TIPIt is recommended to perform an installation/operation qualification (IQ/OQ) forthe instrument. An IQ/OQ ensures that your instrument is complete and in goodworking order before starting work.4.1 Supplied Parts Lovis 2000 MThis is the list of the standard delivery range of Lovis 2000 M for "stand alone"operation. As the real delivery range depends on your order, see also the IQOQ(installation and operation qualification) document you receive during installation.For ordering spare parts refer to the Product Description List (PDL) on the USBflash drive supplied with your Lovis.Table 4.1: Supplied Parts Lovis 2000 MSymbol Pcs. Article Description Mat. No.1 Lovis 2000 M Microviscometer 94075C72IB002EN-E 25

4 Checking the Supplied Parts SECTION I : Lovis 2000 M/ME ReferenceSymbol Pcs. Article Description Mat. No.1 Lovis 2000 M/ME Instruction Manual1 Pack Protection foils for touch-screen (3 pcs.) 814021 Waste Bottle SVM 3000 0.5 L 178221 Power cord (country specific)EUR:GBR:USA:PRC:THAI:1 Accessory Kit Basic-Lovis6514661865526562701179730100219Standard contents see Chapter 16.1.4.2Accessories other than contained in this kitdepend on your orders. Compare all delivereditems to the delivery list respectively to the IQOQdocument.4.2 OptionsSome basic options see Chapter 16.2.For all available options and for ordering consumables or spare parts, consult theLovis 2000 M/ME Product Description List (Doc. No. C72IE001EN) provided onthe USB flash drive.26 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference5 Functional Components5 Functional ComponentsThis chapter contains pictures and short descriptions of all connectors andfunctional components of Lovis 2000 M/ME.5.1 Lovis 2000 MThe following pictures shows only Lovis 2000 M – without DMA M or DSA M. Forthe functional components of these instruments refer to the correspondinginstruction manuals.5.1.1 Lovis 2000 M Front and Left Side View4325671Fig. 5 - 1Lovis 2000 M front and left side1 ... Keys2 ... Color TFT touch screen with disposable protection foil (see Appendix E.2)3 ... Power on LED4 ... Lovis capillary block5 ... Blind covers for inlet and outlet of the optional cooling kit – applicable forDMA M/DSA M only6 ... USB interfaces (3x)7 ... Protection cover for USB interfacesC72IB002EN-E 27

5 Functional Components SECTION I : Lovis 2000 M/ME Reference5.1.2 Lovis 2000 M Right Side View51324Fig. 5 - 2Lovis 2000 M right side1 ... Lovis capillary block2 ... Air pump outlet3 ... Lovis 2000 ME4 ... Lovis 2000 ME fixing screws5 ... Filling Support Luer – for flowthroughfilling with syringe5.1.3 Lovis 2000 M Rear View2 15 341514679813121110Fig. 5 - 3Lovis 2000 M rear view1 ... Lovis capillary block2 ... Capillary block fan3 ... RS-232 interface (COM)4 ... USB interface5 ... VGA interface6 ... Ethernet interface7 ... CAN interface8 ... "DRY AIR IN AIR PUMP" connector – for optional drying cartridge (see Appendix E.1)9 ... "DRY AIR IN INTERNAL" connector – not required for Lovis 2000 M10 ... Power switch11 ... Fuse holder12 ... Power socket13 ... Type plate with serial number14 ... Fan15 ... Carrying ledge28 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference5 Functional Components5.2 Module Housing Lovis ME5.2.1 Module Housing Front View123Fig. 5 - 4Module Housing Lovis ME front view1 ... Power LED (green) – lights up when the power is on2 ... Status LED (orange) – blinks when booting, lights up when all connectionsbetween master instrument and Lovis 2000 ME are correct3 ... Error LED (red) – lights up in case of an error. If a supply cable isinterrupted, it lights up only for a short time. An error message on thedisplay of the master instrument follows.5.2.2 Module Housing Right Side View123Fig. 5 - 5Module Housing Lovis ME right side view1 ... Slot for Lovis 2000 ME microviscometer module2 ... Connector3 ... Threads for fixing screwsC72IB002EN-E 29

5 Functional Components SECTION I : Lovis 2000 M/ME Reference5.2.3 Module Housing Rear View4312Fig. 5 - 6Module Housing Lovis ME rear view1 ... Connector for CAN interface cable to master instrument2 ... Connector for CAN/Devicenet terminating resistor3 ... Type plate with serial number4 ... Unused threads5.3 Operating Elements of the Main Screen1237456Fig. 5 - 7Main screen example – Camera view of U-tube only in combinationwith DMA 4500/5000 M1 ... Header2 ... Output field3 ... Content area4 ... Camera view of U-tube5 ... Progress bar6 ... Buttons area7 ... Quick access buttonsHeader (Fig. 5 - 7: 1)Left side: Name of the currently active method and sample number.Right side: Clock and user indicator. The user indicator indicates the type of userthat is currently logged on (see Appendix C and Instrument Firmware Manual).30 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference5 Functional ComponentsContent area (Fig. 5 - 7: 3)In the content area, the measuring values are displayed in small, medium or largeoutput fields (2). The layout of the content area is defined in the settings of thecurrent method and can be adapted according to your needs (see Appendix Cand Appendix D).A progress bar (5) at the bottom of the content area indicates whether theinstrument is currently measuring or whether a measurement was finished.Buttons area (Fig. 5 - 7: 6)The buttons in this area have the following functions: / aOpen the main menu.Open the quick settings. / Open the current sample list.Open the method list and select a method.Start a measurement.Stop and abort a measurement.a. When a sample list is defined, the button replaces the button .Quick access area (Fig. 5 - 7: 7)Fig. 5 - 8Expanded quick access area• To expand the quick access area, tap the icon on the quick access bar inthe main screen.• To collapse the quick access area, tap the icon on the upper right cornerof the window.• To browse items, use the page navigation in the header ofthe window.• To rearrange, rename or delete the items in the quick access area, tap thebutton (Settings) in the upper left corner of the window. For details, seethe Instrument Firmware Manual.C72IB002EN-E 31

5 Functional Components SECTION I : Lovis 2000 M/ME ReferenceButtonFunctionTo open the message list (see also Appendix F.1).The general instrument status as well as all measuring errors thathave occurred during the measurements of the currently activesample list are described in this list. The button changes itsappearance depending on the current error status :Green OK sign: The general instrument status and the error statusof all measured samples of the current sample list are OK.Yellow warning sign:• The instrument (or system) has a minor problem (e.g. there is aprinter problem etc.).• One or more samples of the currently active sample list had anerror (e.g. too great forward/backward deviation of runtimes).Red error sign:• The instrument (or system) has a major problem that needs tobe fixed before you continue measuring (e.g. no ball detected).• One or more samples of the current sample list could not bemeasured.To reset the message list button to the green check, confirm all errormessages by tapping on the button on the right side of themessage. To confirm all messages in the list in one step, tap the"Delete all" icon at the end of the list.The message list button will also be reset to the green check symbolif you delete the currently active sample list (see Appendix C).U-View TM : To open the live camera view of the measuring cell(DMA 4500/5000 M and DSA 5000 M only). For Lovis 2000 M aloneor in combination with DMA 4100 M this button is inactive andgrayed out.To start the air pump (The air pump is off).To stop the air pump (The air pump is on).The screen is unfrozen, shows live measuring values.If you have started a measurement, the continuous measuringvalues are shown until the measurement is finished. The final valuesare frozen until the next measurement is started. To unfreeze thescreen, tap the below button.To unfreeze the screen after a finished measurement. (The screenis frozen after end of measurement).If you have not started a measurement yet, or if you have terminateda measurement by tapping , the instrument is in the monitormode and shows a continuous reading of the current temperature inthe Lovis capillary block.To display information on using favorites. Only available if no U-View TM -button is available and no favorites have yet been added tothe quick access area by the user.32 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference5 Functional Components5.4 Operating Elements of the Menu ScreenTo access the menu, tap in the main screen.12354687Fig. 5 - 9Menu screen examples1 ... Header2 ... Menu level 13 ... button4 ... button5 ... Content area6 ... button7 ... Buttons area8 ... Content areaOn the left side of the header, you find the navigation path (1) to your currentposition in the menu. You can go back to any menu position in your current pathby directly tapping on the respective box of the navigation path.In the content area (5, 8), you find the menu options of the current menu level andthe menu dialogs.ButtonsButtons at the bottom of screens indeeper menu levelsFunctionMoves to the next higher menu level.Returns to the main screen.Different functions depending on thecurrent menu or dialog.C72IB002EN-E 33

6 Preparing the Instrument SECTION I : Lovis 2000 M/ME Reference6 Preparing the InstrumentThis chapter informs on how to set up the instrument after unpacking it. It doesnot give instructions how to prepare a measurement (e.g. filling a capillary). Itgives specific instructions for Lovis 2000 M/ME. If using Lovis 2000 ME inside aDMA M/DSA M, also read and follow the corresponding instruction manuals.6.1 Installation RequirementsThe setup location and surroundings should meet the requirements of a typicallaboratory.Allow the equipment to reach ambient temperature before installation. This is veryimportant if the equipment has been stored or transported at lower temperatures.Place the instrument on a stable, flat lab desk which is free of vibrations.To ensure temperature stability and trouble free measurement never locate yourinstrument:• next to a heating facility• near an air conditioning, ventilation system or an open window• in direct sunlight• on a vibrating surface or close to vibrating equipmentKeep the instrument away from magnetic fields. Those could possibly influencethe inductive sensors or electronics. Read the Safety Instructions in Chapter 2.Find all Technical Data required for installation in Appendix A.5.WARNINGUsing hazardous or flammable chemicals as samples or cleaning liquids coulddestroy the instrument and cause serious injuries when not taking specialprecautions into account.• See the section "Special precautions for flammable chemicals" inChapter 2 for information on a suitable installation place.NOTICEEnsure that the power plug and the power switch are always freely accessibleso that the instrument can easily be disconnected from the mains at any time.A strong built-in cooling fan dissipates heat through the bottom and the rear ofLovis 2000 M. Ensure that the airflow is not blocked and assure a minimumdistance of 100 mm (3.9 inches) to walls behind and besides the instrument. A second built-in cooling fan dissipates heat through the top of the Loviscapillary block. Do not cover the venting slots.34 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference6 Preparing the Instrument6.2 Mounting Lovis 2000 MCarefully unpack the Lovis 2000 M and place it on the lab desk. Make sure theinstrument stands evenly and that the capillary block can turn freely. While theinstrument is switched off, turn the capillary block manually in both directions asfar as possible and check that is does not touch any obstacles or gets entangledwith hoses, cables or other objects.To connect the silicone hose at the air pump outlet1. Cut a piece of approx. 250 mm (10 in) length from the silicone hosecontained in the accessory kit.2. Attach the silicone hose to the air pump outlet.3. Attach an Adapter Luer Cone (from the accessory kit) to the other end of thesilicone hose.Fig. 6 - 1Lovis 2000 M with air hose plus Luer cone connectedTIPIf the ambient air humidity is high and the measuring temperature is lower thanthe ambient temperature, it is recommended to connect a drying cartridge to theair pump inlet to avoid condensation. See Appendix E.1.C72IB002EN-E 35

6 Preparing the Instrument SECTION I : Lovis 2000 M/ME Reference6.3 Switching Lovis 2000 M On/OffWARNINGHigh voltage at parts of the instrument can cause serious injuries or death.• Only connect the instruments to the mains via protective earthing.• Never connect the instrument to the mains via protective separation orprotective insulation.• Make sure that the non-fused earth conductor of the power cord isconnected to earth.NOTICEBefore switching the instrument on, make sure that the correct line voltage isavailable (AC 100 to 240 V, 50 to 60 Hz). If large voltage fluctuations are to beexpected, we recommend using a constant voltage source (UPS).1. Connect the power socket of the Lovis 2000 M (see Chapter 5.1.3) to themains using the supplied power cord.2. To switch the instrument on, use the power switch on the rear.The green LED on the front side of the Lovis 2000 M indicates that the poweris on. During initialization the Lovis capillary block first moves to -90°, then to0° and stops in the home position (see Chapter 7.1.3).3. After turning on the power, the instrument waits for temperature equilibration.During this time "temp. equilibration" is displayed.4. To switch the instrument off, use the power switch.6.4 Mounting Lovis 2000 ME Module in the MasterInstrument or in the Module Housing Lovis MEIf ordering a Lovis 2000 ME module plus an Xsample module, a ModuleHousing Lovis 2000 ME and a master instrument, all items are packedseparately.In the following, Lovis 2000 M and DMA M/DSA M are indicated as "masterinstrument". Lovis 2000 ME is named "module".TIPIf combining a Lovis 2000 with an Xsample module, mount the Lovis 2000 ME inthe Module Housing and the Xsample module in the master instrument.If mounting the Lovis 2000 ME inside a DMA M/DSA M, you first need to updatethe instrument firmware of the master instrument to the version on the USB flashdrive supplied with the Lovis 2000 ME. Otherwise Error 13 will occur (seeAppendix F.2). To update, refer to the instruction manual of the DMA M/DSA M.36 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference6 Preparing the InstrumentNOTICEIf retrofitting an existing Lovis 2000 M / DMA M / DSA M make sure that themaster instrument is switched off before connecting or disconnecting amodule.TIPKeep the packaging material, the cover of the slot and the bottom plate of themaster instrument (if any) for possible returns or if removing the module again.1. Unpack the master instrument or the Module Housing Lovis ME carefully andplace it on a flat lab desk.2. In case of a DMA /DSA M remove the cover (1) of the slot for the module byunscrewing the two fixing screws M4x20 (2; Torx T20 supplied with Lovisaccessory kit). See Fig. 6 - 2.The Module Housing Lovis ME and the Lovis 2000 M Base Unit comewithout cover.12Fig. 6 - 2Removing the cover of a DMA M/DSA M3. To remove the bottom plate of a DMA M/DSA M (1; Fig. 6 - 3), pull it out.The Module Housing Lovis ME and the Lovis 2000 M Base Unit comewithout bottom plate.2Fig. 6 - 3Removing the bottom plate of a DMA M/DSA M1C72IB002EN-E 37

6 Preparing the Instrument SECTION I : Lovis 2000 M/ME Reference4. Carefully unpack the module (1) and insert it into the slot (2; Fig. 6 - 4) of themaster instrument or of the Module Housing Lovis ME. Push the module slowly backwards until it is in place.12Fig. 6 - 4Inserting the Lovis 2000 ME Microviscometer ModuleWARNINGElectrostatic discharge of a module that is not correctly grounded may causesparking and fire.• To ground the module according to regulations, only use the suppliedscrews with the contact washers for fixing the module in the masterinstrument or in the Module Housing Lovis ME.5. Insert the supplied fixing screws M4x20 (Fig. 6 - 5: 1; Torx T20 supplied withLovis accessory kit) - for Lovis 2000 ME together with the contact washers(2) - into the holes of the module and fix them hand-tight.21 1Fig. 6 - 5Mounting the Lovis 2000 ME module38 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference6 Preparing the Instrument6.5 Adjusting the Foot of the Module HousingLovis MEIf placing a Lovis 2000 ME inside a Module Housing on top of a masterinstrument, the cover of the master instrument may bend slightly due to theinstrument’s weight. Then the left rear foot of the Module Housing (seen from thefront) will possibly lift a little bit. To close the gap place some distance washers (3 pcs. supplied with the Module Housing) inside the foot. Proceed as follows:1. Put the Module Housing on its side. It is not necessary to take out theLovis 2000 ME Module.2. Remove the silicone part (2) of the foot in question (1) with a small flat screwdriver (Fig. 6 - 6).12Fig. 6 - 6Taking apart the left rear foot of Module Housing Lovis ME3. Put one or more distance washers (3) on the center of the foot (Fig. 6 - 7, A).The number of distance washers required depends on how wide the gap is.AB(3 2(Fig. 6 - 7Assembling the left rear foot of Module Housing Lovis ME4. Put the silicone part (2) back onto the foot (Fig. 6 - 7, B).5. Place the Module Housing with the mounted Lovis 2000 ME on top of themaster instrument. All four feet must stand solidly on the master instrument’scover. Add or remove a distance washer on the foot if necessary.C72IB002EN-E 39

6 Preparing the Instrument SECTION I : Lovis 2000 M/ME Reference6.6 Connecting the Cables to Module HousingLovis ME42b321Fig. 6 - 82aConnecting the cables for Module Housing Lovis ME1. Place the Lovis 2000 ME on the master instrument.2. Connect the power cord (1; Fig. 6 - 8) to the power socket of the masterinstrument and to the mains supply.3. Connect the CAN interface cable (2; Fig. 6 - 8):• One end (2a) to the "CAN" interface of the master instrument.• The other end (2b) to the male CAN plug of the Module Housing Lovis ME.• Secure the connectors against accidental loosening by tightening eachsleeve nut finger-tight.• Power supply to the Module Housing Lovis ME and data transfer betweenthe master instrument and the module are performed via this CAN cable.4. Plug the supplied CAN/Devicenet terminating resistor (3; Fig. 6 - 8) to theconnector of the Module Housing Lovis ME (4; Fig. 6 - 8). Tighten the sleevenut finger tight. A measuring assembly consists of several instruments connected in series.This terminating resistor should always be connected to the last instrumentin the series to avoid signal reflections at an open end of the signal line.Note: For all measuring assemblies with more components than a master instrument,Lovis 2000 ME and sample changer, a detailed description of the cableconnections is given in the respective chapters. See SECTION II.40 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference6 Preparing the Instrument6.7 Switching Lovis 2000 ME On/OffLovis 2000 ME is switched on or off via the power switch of the master instrument(DMA M/DSA M or Lovis 2000 M). Refer to the corresponding instruction manual.The Lovis 2000 ME is initialized and waits for temperature equilibration asdescribed in Chapter 6.3.C72IB002EN-E 41

7 Settings and Methods SECTION I : Lovis 2000 M/ME Reference7 Settings and Methods7.1 Measuring System SettingsIn the Measuring System Settings for the Lovis Module you can create, edit ordelete capillary configurations, (de)activate the capillary detection and set thefilling position. A capillary configuration is a capillary with one type of ball.For instrument firmware version 2.32 you can also enter or import data of solventsfor polymer applications.TIPYou can access the measuring systems settings only with administrator ormanager rights.7.1.1 Capillary AdministrationTap "Menu > Setup > Measuring System Settings > Lovis Module > CapillaryAdministration".The list of available capillaries is displayed. Upon delivery this list is empty. Youneed to install at least one capillary to perform a measurement.To add a new capillary1. Tap . There are two possibilities:• Select "Create New Capillary" and tap . See point 2.• Select "Import Capillary Configuration" and tap . See point 3.2. Enter the following data for a new capillary:• Ball type, capillary diameter in [mm] and length, capillary serial number(engraved on the glass surface) and the name of the capillaryconfiguration. Short capillaries (only with instrument firmware 2.32 )see Chapter 3.1.3.• An internal checksum verifies that the capillary serial no. is valid.• Define whether the capillary requires an adjustment.TIPYou can store one capillary with different ball types by using different names.If measuring runtimes and relative viscosity values only, it is not necessary toadjust the capillary. Find more information in Chapter 3.1.1 and Chapter 3.1.2.• Tap to save the settings.3. To import a capillary configuration you need a data file on a USB flash drive.If you have ordered a factory capillary adjustment this file is supplied with thecapillary. You can also use data already stored in another Lovis 2000 M/ME(see below "To export a capillary from the list").42 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference7 Settings and Methods• Select the "Drive" (USB HD) and the "Target" (capillary data).• If data of more than one capillary is stored on the flash drive, select thecapillary/capillaries to be imported from the drop down list.• Tap . You can assign a new name to the capillary and definewhether the capillary requires an adjustment (see "TIP" above).• Tap . The capillary/ball configuration is added to the capillary list.To delete a capillary from the list1. Tap the capillary to highlight it.2. Tap and .To edit a capillary from the list1. Tap the capillary to highlight it and tap .2. The input window for editing a capillary comes up. You can change thecapillary name and set whether an adjustment is required.3. Tap to save your settings.To export a capillary from the list1. Tap the capillary to highlight it and tap .2. The input window for editing a capillary comes up. You can change thecapillary name and set whether an adjustment is required.3. Connect a USB flash drive to the master instrument.4. Tap . Select the "Drive" (USB HD) and tap . The capillary datais exported. A message confirms the successful export. Confirm with .7.1.2 Capillary DetectionIn this submenu you can deactivate the automatic capillary detection.Lovis 2000 M/ME ensures that a Screw Cap Front Side (open or closed) ismounted. If an inductive sensor in the Lovis capillary block does not detect ametal ring on the Screw Cap Front Side, you get the message "Cannot startmeasurement because no capillary is in the capillary block."Lovis 2000 M/ME registers whenever the Screw Cap Front Side has beenremoved and fastened again. When the capillary detection is active,Lovis 2000 M/ME asks whether the currently selected capillary is really the oneinside the Lovis capillary block. If only one capillary/ball configuration is stored inthe instrument, it always uses this one for measurement.TIPYou can always select any installed capillary in the Quick Settings (seeChapter 10.1).By default, the capillary detection is activated. It is recommended to leave it on.C72IB002EN-E 43

7 Settings and Methods SECTION I : Lovis 2000 M/ME Reference1. Tap "Menu > Setup > Measuring System Settings > Lovis Module >Capillary Detection".2. Tap on the check box to activate or deactivate the function.3. Tap to save the new setting.7.1.3 Capillary Filling ModeIn this submenu you can choose between manual capillary filling outside the Loviscapillary block and flow-through capillary filling by syringe (or with an Xsample –refer to p. 110, SECTION II : Flow-Through Systems).The Lovis capillary block assumes the position set in this submenu as homeposition. It goes to this position after activating the instrument and after everymeasurement.• By default, Lovis 2000 M/ME is set to manual filling. In this position the angleof the Lovis capillary block is -45°.• The position for flow-through filling is +20°. The front end of the Loviscapillary block is lower than the back end. During filling, potential air bubblescan rise with the liquid flow and can easily leave the capillary again.1. Tap "Menu > Setup > Measuring System Settings > Lovis Module >Capillary Filling Mode".2. Select :• or• 3. Tap to save the new setting.7.1.4 Polymer Solvents AdministrationThis item is available for instrument firmware version 2.32 only.In this submenu you can enter all solvent data required for testing polymersolutions. The data are stored for future measurements.For additional information on testing polymers with the Lovis 2000 M/ME, consultthe Application Report on Polymer Solutions (Doc. No. C72IA023EN)1. Tap "Menu > Setup > Measuring System Settings > Lovis Module >Polymer Solvents Administration".A list of all stored solvents comes up. By default, this list is empty.2. Tap on the respective parameter fields to enter or change a solvent’s name,its batch number and the measuring temperature. (After entering a newname or batch no., 20.00 °C are suggested as default temperature).44 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference7 Settings and Methods3. Tap the parameter field "Details". A list of different measuring angles for thissolvent and temperature comes up.For newly entered solvents, this list is empty.4. Enter a measuring angle, the solvent’s dynamic and/or kinematic viscosityand/or the runtime (for short/long measuring distance) at this angle.TIPIf you have already measured a polymer solvent with the Lovis 2000 M/MEbeforehand, tap to transfer the solvent’s measurement data from theinstrument’s data memory to the list of polymer solvents.• All measurement results available for the set measuring temperature (seepoint 2.) are displayed.• Scroll through the list, tap on the one you want to select and confirm with.• To enter data for more than one measuring angle, tap a parameter field ofthe empty row or tap again.• Enter or import values as described above.• To delete an angle, tap on the respective row to select it and tap .5. Tap to save your settings.If testing polymer solutions prepared with this solvent, select the stored data in theQuick Settings before starting measurement. It is not necessary to measure thesolvent again for every new Single Concentration or Multi Concentration test.7.2 Lovis Measuring Methods SettingsTo save the user’s time, parameters which need to be set before startingmeasurement are defined in measuring methods. Once a method has beendefined, any user with operator rights can select it for a test.TIPOnly users with administrator or manager rights can create and edit methods.7.2.1 Factory Predefined MethodsLovis 2000 M/ME is delivered with the following factory predefined measuringmethods, which are suitable for the most common applications.By default, the method names are in English. You can rename them in the"Method Management". See the Instrument Firmware Instruction Manual.TIPIf testing polymer solutions use method "Lovis Polymer (Single Conc.)" or "LovisPolymer (Multi Conc.)". Please consult the Application Report on PolymerSolutions (Doc. No. C72IA023EN, supplied on the USB flash drive).C72IB002EN-E 45

7 Settings and Methods SECTION I : Lovis 2000 M/ME ReferenceLovis standardFig. 7 - 1Fig. 7 - 2Example Method Lovis standardExample Method Lovis detailed• Lovis Dynamic Viscosity, Lovis KinematicViscosity, Lovis Average Runtime, LovisVariation Coefficient, Lovis Capillary State,Lovis Temperature, Lovis Density, LovisCondition• Var. Coeff. set by default: 0.10 %• Default measurement mode: S (Standard)• For use without DMA M / DSA M.• If viscosity results are required, the sampledensity must be entered manually in thesample list.• General purpose method.Lovis detailed• Lovis Runtime Details (runtimes of everyforward and backward run), Lovis Density,Lovis Capillary State, Lovis Temperature,Lovis Set Temperature, Lovis Condition,Lovis Shear Rate, Lovis VariationCoefficient, Lovis Forward/BackwardDeviation, Lovis Measuring Length, LovisDynamic Viscosity, Lovis KinematicViscosity.• Var. Coeff. set by default: 0.10 %• Default measurement mode: S (Standard)• For use without DMA M / DSA M• To get viscosity results, the sample densitymust be entered manually in the sample list.• Provides detailed measuring data.Fig. 7 - 3Example Method Lovis / DMALovis / DMA – available only in combination withDMA M/DSA M• Lovis Runtime Details (runtimes of everyforward and backward run), Lovis DynamicViscosity, Lovis Kinematic Viscosity, LovisTemperature, Lovis Capillary State, LovisVariation Coefficient, Lovis Shear Rate,Master Condition, Density, DensityTemperature, U-View• Var. Coeff. set by default: 0.10 %• Default measurement mode: S (Standard)• For use with DMA M/DSA M, the measureddensity is used automatically to calculateviscosity results.• For use with DSA M display sound velocityin one output field (e.g. instead of the shearrate). See the Instrument Firmware Manual.• General purpose method.46 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference7 Settings and MethodsFig. 7 - 4Fig. 7 - 5Example Method Lovis Polymer(Single Conc.)Example Method Lovis Polymer(Multi Conc.)Lovis Polymer (Single Conc.) – especially forpolymer applications, only for instrumentfirmware version 2.32 (see Chapter 3.1.3)• Lovis Runtime Details (runtimes of everyforward and backward run), Lovis VariationCoefficient, Lovis Temperature, LovisCapillary State, Lovis Condition, PolymerConcentration (m/v), Relative Viscosity, K-Value, Reduced Viscosity, Inherent Viscosity,Intrinsic Viscosity (Billmeyer), SpecificViscosity• Var. Coeff. set by default: 0.35 %• Default measurement mode: P (Polymer)• For use with/without DMA M. If available,measured density is used automatically tocalculate viscosity results.Lovis Polymer (Multi Conc.) – especially forpolymer applications, only for instrumentfirmware version 2.32 (see Chapter 3.1.3)• Lovis Runtime Details (runtimes of everyforward and backward run), Lovis VariationCoefficient, Lovis Temperature, LovisCapillary State, Lovis Condition, PolymerConcentration (m/v), Relative Viscosity,Reduced Viscosity, Specific Viscosity,Inherent Viscosity, Intrinsic Viscosity(Kraemer), Molar Mass (Kraemer)• Var. Coeff. set by default: 0.20 %• Default measurement mode: P (Polymer)• For use with/without DMA M. If available,measured density is used automatically tocalculate viscosity results.The following Lovis specific values are identical for the predefined methods. Themeaning of the parameters is explained in Chapter 7.2.5:Temperature Settings Temperature 20 °CTemperature Equilibration 0 sMeasuring settings Measurement Cycles 2Use manual angle check box deactivatedUse variation coefficient checkboxMeasuring distanceAdvanced settings Max. Measurement Cycles 6Auto-distance threshold 60 sAuto-angle threshold20 sMeasuring Timeout250 sactivated; variationcoefficient: for thevalue see theabove methodsAutomaticC72IB002EN-E 47

7 Settings and Methods SECTION I : Lovis 2000 M/ME ReferenceIf you need other method settings to suit your application:• Edit an existing method or• Create a new method7.2.2 Creating, Editing and Deleting Measuring Methods1. Tap "Menu > Methods > Method Management". Select a method from thelist.• Tap to copy a method. Enter a new name for this method. Edit thenew method as described in Chapter 7.2.5.• Tap to assign a new name to method.• Tap to delete a method.You cannot rename or delete the currently used method.To change method parameters that do not only affect Lovis 2000 M/ME, refer tothe Instrument Firmware Manual or to the instruction manual(s) of the otherinstruments/modules of the assembly.7.2.3 Measurement Mode SettingsThis chapter explains the measurement modes which define what kind of test isperformed.1. Tap "Menu > Methods > Method Settings". Select a method ("MethodName") from the list.2. Select a "Measurement Mode (XX)".Lovis Measurement ModesS (Standard)C (Check)MM (MultipleMeasurement)RM (RepeatedMode)To perform a single temperature / single angle test.To perform one of the predefined checks.It is only possible to select a check which has beendefined for the selected method before. Checks aresingle temperature / single angle tests. Refer to theLovis 2000 M/ME Reference Manual for details.To perform a single temperature / single angle testrepeatedly (2 to 10 times, as defined).To perform up to 5 repeated measurements withrefilling liquid from the same syringe or sample vial(if using an Xsample model) without cleaning inbetween.The repeat deviation between determinations can becalculated (see "Repeated Mode Settings").48 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference7 Settings and MethodsMF (Multi Fill) If using an Xsample model: To perform up to 5repeated measurements refilling liquid from the samesyringe or sample vial with automatic cleaning inbetween.If not using an Xsample model: To performup to 5 repeated measurements refilling liquid fromthe same syringe.TSScan)(TemperatureTTS (TemperatureTable Scan)TiS (Time Scan)FAS (Fixed AngleScan)To measure from start to end temperature at setintervals (single angle). The minimum step size is1 °C.To measure at user-defined temperatures (singleangle). It is not necessary to enter the temperaturesin a certain order. The minimum difference betweentwo consecutive temperatures is 1 °C. Do not enteridentical start and end temperatures.To measure for a defined duration at set intervals(single temperature / single angle).To measure from start to stop angle at set intervals(single temperature). The minimum step size in anglescans is 1°.Only available for instrument firmware version 2.32(see Chapter 3.1.3).To measure relative viscosity• either from a polymer solvent and one singleconcentration solutionP (Polymer) a • or from a polymer solvent and at least twosolutions of different concentration (multi conc.). Maximum number of solutions: 10.The measurement is performed at one angle or atseveral angles for extrapolating the viscosity at zeroshear rate. Maximum number of angles: 10.ZSS (Zero ShearScan)Only available for instrument firmware version 2.32(see Chapter 3.1.3).To measure a shear-dependent sample at differentautomatically selected angles. The viscosity at zeroshear rate is extrapolated. To display the Zero ShearViscosity select it as quantity (from parameter group"Lovis Polymer") for one of the output fields (seeInstrument Firmware Manual).By default, the sample is tested at three differentangles. To change the number of angles (max. 10)see Chapter 7.2.5.6.This mode is not suitable for polymer solutions forwhich the concentration is a relevant parameter!a. For details on the Polymer mode see Chapter 7.2.5.6 and Chapter 7.2.5.7 and consultthe Application Report on Polymer Solutions (Doc. No. C72IA023EN, supplied on theUSB flash drive).C72IB002EN-E 49

7 Settings and Methods SECTION I : Lovis 2000 M/ME ReferenceTIPIf defining a TS or FAS, make sure to set the step interval so that the instrumentcan reach the end value by a whole number of steps (divide the interval betweenstart and end value by whole numbers only to find appropriate step settings). Ifyou enter a unsuitable end value, the instrument suggests a different input.Repeated Mode SettingsThese settings only apply to measurement mode "(RM) Repeated Mode".Tap "Menu > Methods > Method Settings > "Method Name" > Repeated ModeSettings".Max. Number OfDeterminationsSave Mode"Active" check box(Lovis AveragesRuntime)"Use Default "check boxMax. Repeat Value[%]The max. number of determinations which shall beperformed to get a valid repeat deviation.Defines which determinations shall be saved (all, validtwo only, or final one only).If activated, the deviation of the mean values of twoconsecutive measurements (determinations) iscalculated and compared to the "Max. Repeat Value".The measurement is valid, if this value is notexceeded. If the max. allowable deviation is exceeded,the instrument can do another determination(depending on the set max. no. of determinations).If activated, the default "Max. Repeat Value" is set.This value is the maximum allowable deviationbetween two determinations. Default: 0.50 %.7.2.4 Formula ParametersThe Formula Parameters can be used if you operate with manual input of thedensity.If you enter the density as Formula Parameters the instrument calculates thedynamic and kinematic viscosity with this/these density value(s) for this method.TIPThe instrument does no longer ask for the input of a density value when startingmeasurement.Mind to enter the correct value(s) if testing samples of different density!You can always change the value(s) in the "Quick Settings" just before startingmeasurement.To set the Lovis Formula Parameters1. Tap "Menu > Methods > Method Settings > "Method Name" > FormulaParameters".2. Enter the "Lovis Density for Start Temperature" for single temperature tests.50 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference7 Settings and Methods3. Enter the "Lovis Density for End Temperature" for temperature or temp. tablescans.If you do no longer need the values in the Formula Parameters, delete them. Thefield "Parameter Select" must be completely empty.7.2.5 Lovis Module SettingsThis submenu contains the following measuring parameters that affect onlyLovis 2000 M/ME :• Temperature Settings• Measuring Settings• Advanced Settings• Density Settings – only for Lovis 2000 ME combined with DMA M/DSA M• Beer/Wort Settings• Polymer Settings – only for instrument firmware version 2.32• Molar Mass Calculation Settings – only for instrument firmware version 2.327.2.5.1 Temperature SettingsSet the measuring temperature and the temperature equilibration time.1. Tap "Menu > Methods > Method Settings > "Method Name" > LovisModule > Temperature Settings".2. Enter the "Measuring Temperature" in the selected unit [°C / °F / K].3. Define the "Temperature Equilibration" time in [s]. This is the time theinstrument waits for the sample to reach the measuring temperature afterstarting measurement and after the Lovis capillary block has reached the settemperature.TIPYou can set the temperature equilibration time to 0 s and use the variationcoefficient (Chapter 7.2.5.2) for temperature equilibration of the sample. Usingthe var. coeff. for temperature equilibrium is not recommended for highly viscoussamples. Due to the long runtimes the procedure would consume too much time.4. Tap to save your settings.7.2.5.2 Measuring Settings1. Tap "Menu > Methods > Method Settings > "Method Name" > LovisModule > Measuring Settings".C72IB002EN-E 51

7 Settings and Methods SECTION I : Lovis 2000 M/ME Reference2. Set the following parameters:• Measurement Cycles:One measuring cycle represents one forward and one backward run. Sethere the number of cycles for calculating the variation coefficient.• Use Manual Angle:If selected, you can define the measuring angle to be used for a test. If youdeactivate the check box, the first measurement will be done using a 70°angle and then the angle is automatically chosen depending on the values inthe Advanced Settings (Chapter 7.2.5.3).• Variation Coefficient:If using the variation coefficient, Lovis 2000 M/ME compares the set value tothe runtime variation coefficient calculated from the set number ofmeasurement cycles. If the calculated var. coeff. exceeds the set value,Lovis 2000 M/ME discards the first cycle and performs another cycle. Themaximum number of cycles within which the set value should be achieved isset in the Advanced Settings (Chapter 7.2.5.3). The var. coeff. provides away to ensure temperature equilibrium of the sample. As long as the sampletemperature is not stable, the runtimes vary between measurement cycles.TIP • The variation coefficient is always calculated by the instrument, but the setlimit value is only applied to a measurement if activated in these settings!• For highly viscous samples (long runtimes) it is recommended to set a fixedtemperature equilibrium time (Chapter 7.2.5.1).• Measuring Distance:Use the drop-down box to select one of the options:• Long: corresponds to 100 mm measuring distance• Short: corresponds to 25 mm measuring distance• Automatic: Lovis 2000 M/ME switches to "Short" if the runtime exceedsthe Auto-distance threshold in the Advanced Settings (Chapter 7.2.5.3).The long measuring distance is recommended for low viscous media to get along enough runtime for optimum precision. Use the short measuringdistance for more viscous samples to save time.3. Tap to save your settings.7.2.5.3 Advanced Settings1. Tap "Menu > Methods > Method Settings > "Method Name" > LovisModule > Advanced Settings".52 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference7 Settings and Methods2. Set the following parameters:• Max. Measurement Cycles:The maximum number of measuring cycles within which the set variationcoefficient (see Chapter 7.2.5.2) should be achieved.• Auto-distance threshold:The auto-distance threshold is that runtime value at which the instrumentswitches from the long measuring distance to the short measuring distance.It is applied only if the option "Automatic" is selected for the measuringdistance (see Chapter 7.2.5.2).• Auto-angle thresholdThe auto-angle threshold is that runtime value at which the instrumentswitches to flatter measuring angles (start angle = 70.00°. It is applied only ifthe option "Use Manual Angle" is not activated (see Chapter 7.2.5.2).• Measuring Timeout (for long distance)The timeout limit is that time in seconds after which a Lovis measurement isaborted if not all coils for the long measuring distance have been passed bythe ball for one measuring run. Set a value between 60 s or 1700 s.In case an application always yields short runtimes, setting this timeout to avalue slightly longer than the expected runtime avoids unnecessary waitingtimes in case of an error (e.g. ball blocked by an air bubble).For testing samples of higher viscosity, the limit must be raised accordingly.The maximum values can be calculated from the capillary measuring ranges(Appendix A.2).The following example demonstrates how to find a suitable timeout for asample:The default measuring timeout of 250 s permits to measure viscosities ofapprox. 1500 mPa.s with the 2.5 mm capillary at an 80° angle.• Extended range of 2.5 mm capillary: max. viscosity = 10 000 mPa.s• This corresponds to 1700 s runtime.• (10 000 mPa.s / 1700 s) * 250 s = 1470.58 s => approx. 1500 mPa.sTIPFor capillary adjustments and the Level Adjustment always the maximum timeoutof 1700 s is applied, independent of the value set for the current measuringmethod.3. Tap to save your settings.7.2.5.4 Density Settings1. Tap "Menu > Methods > Method Settings > "Method Name" > LovisModule > Density Settings".C72IB002EN-E 53

7 Settings and Methods SECTION I : Lovis 2000 M/ME ReferenceThis menu item is available only for Lovis 2000 ME combined with DMA M/DSA M.2. Set the following parameters:• Density Source:Select the density value for calculating dynamic or kinematic viscosity:• DMA Density (default setting) – use the measured density value• User Function – use calculated density values (see below)• Lovis Density (Manual Input) – enter the density manually• User Function:Active only if selected as "Density Source". The instrument calculatesspecial density values from the density measured by the DMA M/DSA M:• Apparent Density Steel Apparent density referring to scales adjusted with brass weights.• Apparent Density Brass Apparent density referring to scales adjusted with steel weights.3. Tap to save your settings.7.2.5.5 Beer/Wort SettingsHere you can enter a specific parameter – the Extract – for testing beer and wort.1. Tap "Menu > Methods > Method Settings > "Method Name" > LovisModule > Beer/Wort Settings".2. Open the drop down list. Select an option:• Approximate Apparent ExtractThe instrument uses a MEBAK polynomial (see MEBAK Handbook 4thedition, chapter 2.10.) to calculate the Approximate Apparent Extract fromthe density value. It does not matter whether the density was enteredmanually or taken from a DMA M/DSA M. (MEBAK is an established Middle-European association for brewing techniques).TIPThe Approximate Apparent Extract applies only to wort. Do not use it if testingbeer. Test results would be faulty.• Manual Input – to enter an Extract value manually.3. Tap to save your settings.7.2.5.6 Polymer SettingsHere you can enter specific parameters for testing polymer solutions. Consult theApplication Report on Polymer Solutions (Doc. No. C72IA023EN, supplied on theUSB flash drive) for details.54 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference7 Settings and Methods1. Tap "Menu > Methods > Method Settings > "Method Name" > LovisModule > Polymer Settings".2. Enter the following parameters:• Calculation MethodAll polymer-related calculations are based on the relative viscosity. Definehere how to calculate the relative viscosity:• by Runtime ratio• by Dynamic viscosity ratio• by Kinematic viscosity ratio• Angle Count (for Zero Shear Scan)Enter the number of measuring angles at which the polymer solution shall betested. This input serves for Zero Shear Scans to extrapolate the zero shearviscosity. If testing a polymer solution at one angle only, this input does notaffect the measurement. For a scan, a minimum of two angles is required.The maximum number of angles is 10. The default setting is 3.• By default, the instrument automatically chooses the angles that areused for measurement.• If using data from the "Polymer Solvents Administration", Chapter 7.1.4,the instruments selects angles that are available for the specifiedsolvent.TIPThe "Angle Count" setting also defines the number of angles when performing ameasurement with mode "ZSS (Zero Shear Scan)". For this mode the measuringangles are always automatically selected.Mind that the measurement mode ZSS is NOT suitable for testing polymersolutions, use the mode P (Polymer) instead!• Use Zero Shear Scan (for each solution)Check this box to perform a Zero Shear Scan for polymer solutions. If you doa Multi Concentration measurement (testing a polymer in solutions withdifferent concentrations), each solution is tested at each angle.If the box is not active, each polymer solution is tested at one single angleonly.3. Tap to continue your input.4. Select a Concentration Input Mode:• Manual inputEnter the concentration(s) of your polymer solution(s) manually in the QuickSettings before starting measurement.• Calculated by user functionC72IB002EN-E 55

7 Settings and Methods SECTION I : Lovis 2000 M/ME ReferenceSelect a "User Function for concentration" that calculates theconcentration(s) of your polymer solution(s). You can only select userfunctions that you have programmed beforehand in the "Setup > ExpertSettings > User Function Management". Refer to the Instrument FirmwareManual for entering user functions.5. Select a Concentration Unit from the drop down list. This option is only activefor manual input mode.6. Tap to continue your input.7. Select one of the calculations for the intrinsic viscosity. If required, enter the correct k (slope) constant. Only select such acalculation if the k (slope) constant is known.8. Tap to save your settings.7.2.5.7 Molar Mass Calculation SettingsIf known, you can enter the coefficients for the Mark-Houwink equation here. Thisequation calculates the molar mass of your polymer.1. Tap "Menu > Methods > Method Settings > "Method Name" > LovisModule > Molar Mass Calculation Settings".2. Enter the Mark-Houwink coefficients:• Mark-Houwink Factor (K)• Mark-Houwink Coefficient (a)3. Tap to save your settings.56 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference8 Checks and Adjustments for Lovis 2000 M/ME8 Checks and Adjustments for Lovis 2000 M/ME8.1 DefinitionsCheckingChecking the correct state of operation of an instrument by measuring a sampleof exactly known measurement properties and comparing the result with theexpected values.Lovis 2000 M/ME allows you to perform tests in the measuring mode "Check". Fordetails, refer to Chapter 8.2.AdjustingEnabling correct measurements in the future by measuring a sample of exactlyknown measurement properties (standard) and adjusting the instrumentconstants in a way that the known correct results are found by the instrument.Lovis 2000 M/ME requires the following adjustments:• Level Adjustment (Chapter 8.3)• Adjustments of capillary configurations (Chapter 8.4)• Temperature Adjustment (Chapter 8.5). The instrument is temperatureadjusted before delivery and users need not do this adjustment as a rule.CalibratingCalibrations are checking procedures which are carried out using certifiedstandards. By comparing the measured result with the standard reference value,you can validate the quality of your measurements.C72IB002EN-E 57

8 Checks and Adjustments for Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference8.2 Checks8.2.1 Editing the Check Settings for Lovis 2000 M/MEYou can edit the factory predefined water check (name, time interval andallowable tolerance) or create an entirely new custom check.NOTICEReference values and limits of custom checks are valid only for onetemperature. Make sure that the currently set measuring temperature of theLovis capillary block agrees with the reference value.If using water as check standard, Lovis 2000 M/ME automatically calculatesthe correct reference value for the currently set Lovis temperature. This is notpossible with customer reference fluids.Factory default tolerance values of the Water Check• Reference value: Kinematic viscosity [mm 2 /s] and dynamic viscosity [mPa.s]of water at the currently set temperature of the Lovis capillary block• Lower deviation: 0.01000 mm 2 /s; 0.01000 mPa.s• Upper deviation: 0.01000 mm 2 /s; 0.01000 mPa.sTo edit the settings of the Water Check1. Tap on the button and select "Setup > Expert Settings > CheckManagement" to open the check management list.2. Highlight the "Water Check" and tap to open the three-step "CheckEdit" wizard.3. Enter a name for the check. Tap .4. Use the check boxes...• to set that the check is "GxP relevant"• to activate a reminder for regular check execution. Enter a time interval forthe check execution reminder: "Remind every" xx day(s). Tap .5. Define for which modules the check should be active. There are differentfields for dynamic and kinematic viscosity. The reference values for water arepre-programmed. Set the maximum allowed lower and upper deviation(absolute values).6. Tap .58 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference8 Checks and Adjustments for Lovis 2000 M/METo create a custom check1. Tap on the button and select "Setup > Expert Settings > CheckManagement" to open the check management list.2. Tap to open the three-step "Check Administration" wizard.3. Perform the following settings:• Enter a name for the check.• Select the method with which you want to perform the check. You canchoose any pre-defined or customized method.Tap .4. Select a quantity for the check.• Select the quantity from Group "Lovis".• If there are several unit applicable to this quantity, select one. Tap .5. Enter the absolute lower and upper limit of the allowable range for the checkresult.6. If required, repeat the two above steps to add more quantities to the check.7. Tap .To edit a custom check1. Tap on the button and select "Setup > Expert Settings > ChecksManagement" to open the check administration list.2. Highlight a custom check and tap to open the three-step "CheckAdministration" wizard.3. Change the settings as required. See "To create a custom check".8.2.2 Performing ChecksWith water or custom checks, carried out in regular intervals, you can ensure ahigh and stable accuracy of your viscosity measurements.For a check, you fill your capillary with a reference standard of known viscosityand compare the measured viscosity with the reference value.The Lovis performs the check automatically at the measuring temperature of themethod that is currently active. The viscosity of water at the measuringtemperature is calculated and compared with the reference value.When to do checksUse the check to verify that the instrument is measuring with satisfactoryaccuracy. We recommend performing a check every day before starting themeasurements. Perform additional checks on demand depending on yourjudgement, e.g. when you get unexpected results.C72IB002EN-E 59

8 Checks and Adjustments for Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME ReferenceTo perform a check1. Tap . Select "Checks/Adjustments > Checks".2. Highlight a list item and tap .3. Follow the instructions on the screen.For a water check use freshly degassed ultra pure (bi-distilled or deionized) water.For a custom check use a reference standard of known viscosity.TIPIf NOT using water make sure that the currently set Lovis temperature agrees withthe reference value and limit value predefined for the check!When the check is finished, the following information is displayed:• Check type• Date and time• Used method• User name• Reference value – for all quantities of the check• Allowable range (lower and upper value) – for all quantities of the check• Measured value – for all quantities of the check• Check result ("Passed" or "Not passed") – for all quantities of the check4. Tap if you want to print or export the check results.5. Tap or to exit the density check.If the check failed, we recommend taking corrective actions until the check is validagain:• Check the quality of the water/reference standard.• Clean the capillary efficiently (see Chapter 11.2).• If the above mentioned actions do not help, perform new adjustment of thecapillary (see Chapter 8.4).8.2.3 Viewing, Printing or Exporting Current Check DataThe total number of entries in the check history is limited to 100.1. Tap and select "Data Memory > Check Data".2. Highlight a list item and tap .3. To perform a printout on paper or to a PDF file or to export the data as anMS Excel or Text file tap . Follow the instructions on thescreen.60 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference8 Checks and Adjustments for Lovis 2000 M/ME8.3 Performing a Level AdjustmentIdeally, the ball’s runtimes of a forth and back run at the same angle should beidentical when the Lovis 2000 M/ME stands levelly on a flat surface. As theinstruments cannot be levelled mechanically by adjustable feet, possibleunevenness of the location of installation should be compensated by performinga Level Adjustment.TIPPerform a Level AdjustmentPerform a Level Adjustment• when initially installing Lovis 2000 M/ME• whenever the instrument has been moved (even if only a bit)• before adjusting a capillary. In this case, ideally perform the LevelAdjustment with the capillary you plan to adjust.For a correct adjustment a correctly filled capillary with a ball is required. Thecapillary can be filled outside the capillary block or by the flow-through method(see Chapter 9). The capillary need not be adjusted for a Level Adjustment!Select a capillary/ball configuration and fill it with a suitable liquid (see AppendixA.2).NOTICEIt is not possible to perform a Level Adjustment with air, as the minimum runtime must not fall below 10 seconds. Further the ball’s friction in an emptycapillary does not represent the conditions in a liquid filled capillary.To perform a Level Adjustment1. Tap and select "Checks / Adjustments > Other Adjustments >Lovis Module > Level Adjustment".2. For instrument firmware version 2.32 only: Select an angle for the Level Adjustment and define the allowable VariationCoefficient. Tap .3. If more than one capillary/ball configuration is installed (see Chapter 7.1.1),Lovis 2000 M/ME prompts you to select a capillary from the list.During the Level Adjustment Lovis 2000 M/ME displays the currenttemperature and the progress.When finished, the instrument shows the results for short and longmeasuring distance:• Required angle correction to achieve near identical runtimes• Average forward and backward runtimes• Forward/Backward Deviation and Variation CoefficientThe Variation Coefficient is shown for instrument firmware version 2.32 only.C72IB002EN-E 61

8 Checks and Adjustments for Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference4. An arrow suggests the suitable action:• If the correction is greater than 0.5°, a message on the lower left-hand sideof the instrument screen reads "Check Result. Improve". The LevelAdjustment should be repeated with the same filling of the capillary. Tap . The first correction is applied and a new adjustment isstarted to fine tune the angle correction.• For corrections smaller than 0.5°, Lovis 2000 M/ME suggests to the Level Adjustment.• Tap to discard the displayed correction and end the adjustment.TIPThe result can be improved several times (even if Lovis 2000 M/ME suggests). It is possible to achieve angle corrections < 0.1°.5. If after improving several times Lovis does not allow you to , checkthe filling of the capillary for bubbles and check that the Lovis stands in astable position. Refill the capillary and tap .6. The Level Adjustment is valid and stored after tapping .8.4 Adjusting Lovis 2000 M/ME CapillariesLovis 2000 M/ME calculates dynamic and kinematic viscosity from theadjustment data of the capillary/ball configuration and from the measuredruntimes.TIPIf you have ordered a factory adjustment, import the adjustment from the USBflash drive delivered with the capillary. See Chapter 7.1.1!Do not lose the original adjustment ball (see below)!Adjustment BallDue to manufacturing the diameters of the supplied balls vary within a definedtolerance.• The variation is greater for gold balls because of the additional layers(undercoating + gold layer) on the steel balls.• The smaller the inner diameter of a capillary is, the narrower is the gap insidethe capillary and the more the ball diameter affects the adjustment results.The below table shows the maximum measurement deviation if changing ballsfrom the same batch.Capillary Steel Ball Gold Ball1.59 mm 0.7 % 6.0 %1.8 mm 0.2 % 1.6 %2.5 mm

SECTION I : Lovis 2000 M/ME Reference8 Checks and Adjustments for Lovis 2000 M/MEDo not change the ball unless the expected deviation is acceptable for you. Thedeviation does not influence relative viscosity results (It is cancelled out whencalculating these values).If you need to change the ball and the expected deviation exceeds your precisionrequirements, perform a measurement with the new ball and a viscosity standardliquid to check the real deviation. Use measurement mode "Standard" or "Check"(for details on mode "Check" see the Lovis 2000 M/ME Reference Manual).If the deviation of the check measurement exceeds your limits, the capillary hasto be re-adjusted with the new ball.TIPIf you plan to dispose of the balls after each measurement do not use gold balls.To adjust a capillaryTo adjust a capillary, you need a reference standard with certified values ofdynamic viscosity and density at the respective temperature. The viscosity of thereference standard must be in the core range of the capillary (see Appendix A.2).A table in Appendix A.3 lists suitable standard liquids.1. Perform a Level Adjustment. See Chapter 8.3.2. Tap and select "Checks / Adjustments > Other Adjustments >Lovis Module". Select:• "Single Point Adjustment" –> for one user-defined angle• "Standard Adjustment" –> for a range of 20° to 70° angle• "Extended Adjustment" –> for a range of 15° to 80° anglePreferably use Single Point Adjustment or Standard Adjustment. Only do anExtended Adjustment if you really need to measure at angles 70°!3. Follow the instructions on the display. Select the capillary (from the list ofstored capillary configurations, see Chapter 7.1.1). Enter the ball batch no.,the ball density (see label of the ball vessel) and the adjustment temperature.TIPYou get the most precise measuring results if adjusting a capillary at or near thattemperature you wish to measure at. To adjust the same capillary at differenttemperatures you can store it by different names in the Capillary Administration.4. Fill the capillary with your standard liquid. You can fill outside the block orwith the flow-through method.5. Enter the standard liquid ("Reference Medium Type") and its dynamicviscosity and density at the adjustment temperature. By default Lovis 2000 M/ME uses the pre-programmed values for water.6. Start the adjustment with .C72IB002EN-E 63

8 Checks and Adjustments for Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME ReferenceWhen finished, Lovis 2000 M/ME displays the ball runtimes at the adjustedangle(s) and the maximal variation coefficient that occurred during theadjustment. If one or more angles are invalid, the respective angles are notstored. The instrument displays a message.7. Tap to make sure the adjustment was correct.During a verification Lovis 2000 M/ME performs the full adjustment procedure fora second time.8. Confirm the allowed deviation between adjustment and verification (default0.5 %).9. Fill the capillary once again with your standard liquid. Do NOT change theball. Follow the instructions on the display.TIPAfter a verification the runtime deviations to the values of the preceding originaladjustment are displayed for each adjusted angle. Typically, these values arehigher than the maximal variation coefficient of the original adjustment.• For verification results within the specifications Lovis 2000 M/ME promptsyou to tap . stores the data of the verification asadjustment data of this capillary configuration.• If the maximal deviation exceeds the allowed deviation, Lovis 2000 M/ME prompts you to tap .• Fill the capillary again (using the same ball) and repeat the verification.• If still exceeding the allowed deviation after the second , clean andbrush the capillary (see Chapter 11.2), refill it and start a new adjustment.10. To print or export an adjustment report, tap .8.5 Temperature AdjustmentA temperature adjustment is required if the temperature calibrationmeasurements are repeatedly outside the limit values. The new adjustment canbe carried out by authorized service personnel or the operator.The adjustment is performed at 20 °C, 40 °C, 60 °C, 80 °C and 100 °C. For amanual adjustment the values are measured by an external thermometer andhave to be entered manually. If doing an adjustment with MKT 50, the values areentered automatically.8.5.1 Performing a Manual Temperature AdjustmentRequired thermometer specification: For temperature adjustment werecommend the CKT 100 high-precision thermometer or a similar device with Pt-100 temperature probe and an uncertainty of measurement of max. 0.01°C.64 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference8 Checks and Adjustments for Lovis 2000 M/METIPCheck the precision and the calibration of the thermometer and the temperatureprobes before starting the temperature adjustment.Required parts for the Lovis:• Pt 100 Adapter Lovis (Mat. No. 98604)• Screw Cap Front Side Open (Mat. No. 98166)To insert the temperature probe1. Lead the Pt-100 temperature probe through the hole of the Screw Cap FrontSide Open.2. Take the Pt 100 Adapter Lovis and unscrew it.3. First lead the Pt-100 temperature probe through the hole of the PEEK part ofthe Pt 100 Adapter Lovis.4. Then carefully insert the Pt-100 temperature probe into the metal part of thePt 100 Adapter Lovis.PEEK partmetal partScrew Cap Front Side OpenPt-100 temperature probeFig. 8 - 6Inserting the Pt-100 temperature probe5. Take care that the recess of the Pt-100 temperature probe fits into thecorresponding clip of the Pt 100 Adapter Lovis.clip of the PT 100Adapter Lovisrecess of Pt-100temperature probeFig. 8 - 7Temperature probe and adapter - recess fitting into the clip6. Screw the two parts (PEEK and metal) of the Pt 100 Adapter Lovis together.7. Put the Pt 100 Adapter Lovis with the inserted Pt-100 temperature probe intothe Lovis capillary block and close the capillary block with the Screw CapFront Side Open.C72IB002EN-E 65

8 Checks and Adjustments for Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference8. On the back side close the capillary block with the Screw Cap Back Side -Closed (Mat. No. 98175).9. Connect the cable of the Pt-100 temperature probe with your thermometer.10. To use the thermometer refer to the corresponding instruction manual.To perform a temperature adjustmentTap and select "Checks/Adjustments > Other Adjustments > LovisModule > Temperature Adjustment".An ongoing adjustment can always be aborted by pressing .1. The instrument prompts you to fix the external temperature sensor to thecapillary block and to tap .2. Wait until the temperatures of the Lovis and the external thermometer arestable.3. When ready, the screen displays the current "Set Temperature". Enter thevalue displayed by the external thermometer (field "ReferenceTemperature").4. The instrument heats to the second temperature value.5. Repeat this procedure until the last temperature value (100°C) is reached.6. After entering the last temperature value, the instrument displays alltemperatures that were successfully measured and prompts you to the results.• To save the temperature adjustment results tap .• To discard the temperature adjustment results tap .• To print the temperature adjustment results press .7. After the adjustment was finished the instrument automatically cools down.CAUTIONThe temperature probe and adapter could be hot after the adjustment whichcould lead to burns to your skin.To prevent injury, allow the instrument to cool down to room temperature beforeremoving these items.8.5.2 Performing a Temperature Adjustment with MKT 50The adjustment is performed at 20 °C, 40 °C, 60 °C, 80 °C and 100 °C, thetemperature values, measured by the MKT 50, are entered automatically.66 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference8 Checks and Adjustments for Lovis 2000 M/MERequired parts:• PT 100 Adapter Lovis (Mat. No. 98604)• Screw Cap Front Side Open (Mat. No. 98166)• MKT 50 Millikelvin Thermometer (Mat. No. 26878)• Sensor for temperature calibration DMA/SVM Pt 100 (Mat. No. 74557)• Ethernet cable for PC connection (Mat. No. 18597)To insert the temperature probe into the Lovis capillary blockRefer to chapter 8.5.1 "Performing a Temperature Adjustment".TIPFor details how to use the MKT 50 refer to the MKT 50 Instruction Manual.To perform a temperature adjustment with an MKT 501. Connect the MKT 50 via Ethernet cable to the Lovis.2. Connect the cable of the Pt-100 temperature probe to Channel (CH1) of theMKT 50 and switch the MKT 50 on.temperatureprobeMKT 50Fig. 8 - 8 Connecting the temperature probe with the MKT 503. To start the adjustment tap "Menu > Checks/Adjustments > OtherAdjustments > Lovis Module > Temperature Adjustment with MKT 50".4. The instrument prompts you to fix the external temperature sensor to thecapillary block and to tap . Instructions come up on the screen.You have already connected MKT 50 (see above). Set the required IPaddress at the MKT 50:• Tap for the main menu and "2 Select Display - 5 Ethernet".• The webserver must be "ON", enter "IP 010.000.000.002".Select the following settings for the MKT 50:• "1 Select Display - 4 Temperature Statistics N: 50".C72IB002EN-E 67

8 Checks and Adjustments for Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference5. Wait till the mean value is displayed on the MKT 50:CH1 20.0057 °CMean : 20.00572°CS.Dev: 0.00018°CN:50/50CH2 No:00008389SENSOR NOT CONNECTEDN:00/50 lineFig. 8 - 9Mean value on MKT 50 display (Channel 1) – Example6. Tap . The temperature adjustment is performed automatically.After measuring the last value, the instrument displays all successfullymeasured temperatures and prompts you to the results.• To save the temperature adjustment results tap .• To discard the temperature adjustment results tap .• To print the temperature adjustment results press .7. After the adjustment was finished the instrument automatically cools down.CAUTIONThe temperature probe and adapter could be hot after the adjustment whichcould lead to burns to your skin.To prevent injury, allow the instrument to cooldown to room temperature before removing these items.8.5.3 Resetting the Temperature Factory Adjustment DataYou can re-activate the factory temperature adjustment.1. Tap and select "Checks/Adjustments > Other Adjustments >Lovis Module > Reset To Temperature Factory Adjustment".2. Activate the check box "Lovis" and tap .8.6 Viewing, Printing or Exporting Adjustment DataView, print and export the detailed data of the last 50 adjustments.Tap and select "Data Memory > Adjustment Data > Lovis Module"and select "Capillary Adjustment", "Temperature Adjustment", "LevelAdjustment" or "Distance Adjustment" to open the respective adjustment data.TIPThe Distance Adjustment is done at works before delivery and need not be doneby the user. The valid results can be viewed.1. Highlight a list item (if applicable) and tap to see the detailedadjustment data.2. To perform a printout on paper or to a PDF file or to export the data as an MSExcel or Text file tap . Follow the instructions on the screen.68 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/ME9 Filling the Lovis 2000 M/METhis chapter describes the possible methods for manually filling a capillary for Lovis 2000 M/ME. How to fill a Lovis 2000 M/ME automatically with an Xsamplesample changer and how to fill Lovis measuring assemblies with other modulesis described in SECTION II : Flow-Through Systems.Either fill a Lovis capillary outside the capillary block or use the flow-through fillingsystem.TIPShort capillaries (low sample volume) can only be filled outside the capillary block.9.1 Selecting a Capillary and a BallSelect a capillary with a suitable diameter and a suitable ball (see Appendix A.2)and the desired length (standard or short).The capillary and the ball should be clean and dry (capillary: inside and outside).NOTICEAll capillaries are factory cleaned before shipping them. Still, efficient cleaningbefore the first filling and measurement is essential to condition the glasssurface! For cleaning instructions, see Chapter 11.1, Chapter 11.2.2 andChapter 11.3.WARNINGCapillaries are made of glass and will break if treated with excessive force.Sharp pieces can cut your hands and fingers. In case of hazardous sampleliquids (acid, caustic, infectious, easily inflammable...) spills and fumes are anadditional health risk. Handle the capillaries with care! Refer to the SafetyInstructions, Chapter 2, for the necessary precautions and for correct handlingof hazardous substances.9.2 Filling a Standard Capillary Outside theCapillary Block1. Take a clean new syringe. Recommended volume: 1 mL or 2 mLNOTICEDo not use syringes that contain lubricants or rubber sealings. Thesematerials can dissolve into your sample and lead to a systematicmeasurement error.C72IB002EN-E 69

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference2. Take the capillary and put a Filling Adapter Luer Female on its top end (nearthe twist protection). Press the adapter downwards and turn the capillary till itclicks into place.Fig. 9 - 1Mounting the Filling Adapter Luer Female3. Hold the free end of the capillary upwards and place the funnel (part ofstandard supply) or the ball dispenser on top of the capillary.4. Take the clean ball with the plastic tweezers and drop it into the funnel. Donot touch the ball with bare fingers.TIPIf using a factory adjustment ball (see Chapter 8.4), tip it directly from its containerinto the funnel.5. If using the ball dispenser, press the release button once to drop a ball intothe capillary.ABFig. 9 - 2 Inserting a ball with tweezers and funnel (A) or ball dispenser (B)6. Put a Filling and Cleaning Adapter Luer Male onto the free capillary end.Proceed as described for the first adapter. The ball can no longer drop fromthe capillary now.Fig. 9 - 3Empty capillary with two adapters and ball (see arrow)There are two possibilities how to fill the capillary: Either you draw in sample withthe syringe directly from a vessel or you fill the syringe first and then fill thecapillary from below, pressing the sample upwards. Consider that the lattermethod involves sample flowing out at the capillary end.70 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/MEDrawing in sample from a vessel1. Plug the tip of the syringe firmly into the FillingAdapter Luer Female.2. Plunge the inlet of the Filling And CleaningAdapter Luer Male directly into the sample liquid.Alternatively, you can fix a hollow needle to thisadapter. This way you can fill samples directlyfrom sealed vessels (by piercing the cover) orfrom narrow vials.3. Place the inlet or the needle well below theliquid’s surface in order to avoid drawing in toomany air bubbles. Slowly draw in sample bymoving the plunger upwards.Draw in sample until the liquid reaches thesyringe and no air bubbles are inside thecapillary.Fig. 9 - 4 Drawing in sample from a vessel4. Leave the syringe on the adapter and remove the capillary from the liquid.Turn the capillary upside down. Closely check the entire capillary for airbubbles.Filling the capillary from the syringe1. Fill the syringe with sample. Wipe the tip of thesyringe with a soft tissue to remove sampleresidues.2. Plug the tip of the syringe firmly into the FillingAdapter Luer Female.3. Turn the capillary upside down so that thesyringe is below it and fill it from bottom to top.Slowly fill the sample by pushing the plungerupwards.Fill sample until the liquid reaches the tip of theFilling And Cleaning Adapter Luer Male and anyair bubbles have left the capillary.Fig. 9 - 5 Filling the capillary from belowC72IB002EN-E 71

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME ReferenceChecking for air bubblesNo matter how you fill the capillary, always check it for air bubbles.1. Hold the capillary upside down (syringe beneath thecapillary).2. Turn the capillary by 360° around its longitudinal axisand look it closely up and down. Tilt it a bit if necessary.360°3. If detecting a bubble, wait till it rises to the top (lightlyknock a finger against the capillary wall if a bubble getsattached) and push the plunger upwards a bit to removeit.4. In case an air bubble is attached to the ball, also loosenit by lightly knocking against the capillary wall. If thebubble is below the ball, turn the capillary around and letthe bubble rise towards the Filling Adapter Luer Female.Wait long enough for the bubble to leave the capillary.Fig. 9 - 6 Removing air bubbles by knocking with a fingerClosing the capillary1. Hold the capillary upside down (syringe beneaththe capillary). Remove the Filling And CleaningAdapter Luer Male (plus hollow needle ifapplicable).2. Select a suitable clean plug for the capillary andclose it. Press the plug firmly into the capillary.Make sure not to insert air bubbles with the plug(tilt the plug slightly when first inserting it). Turn the plug to insert it deeper into the capillary.Fig. 9 - 7Inserting the plug72 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/ME3. Turn the capillary (twist protection up) andremove the syringe from the adapter.4. Firmly press a Luer plug (with or withoutbore) into the Filling Adapter’s opening.Fig. 9 - 8Fastening the Luer plugThe capillary should now be filled air-bubble free and closed tightly. Carefullycheck it for air bubbles again – they possibly stick to the ball or the tip of the plug.Check that the ball rolls freely in both directions. Air bubbles will slow down theball and cause faulty measurement results.Fig. 9 - 9Filled Capillary with ball (see arrow)TIPNote down the serial number of the capillary. You need it when entering thecapillary data in the instrument’s "Capillary Administration".9.3 Filling a Short CapillaryYou can fill short capillaries outside the capillary block only.Syringes and hollow needles are supplied with the low volume filling sets. Select a suitable syringe and needle diameter depending on the sample viscosityand volume.• Plastic syringes are disposable items.• For precise dosage and minimum loss of sample volume use the microliterglass syringe and the hollow needle 0.7 (gauge 22).• Pull the plunger of the microliter syringe only as far out as the µL-scale.TIPThe supplied hollow plugs for closing short capillaries are factory-cleaned beforedelivery, but there may still be particles inside that would influence themeasurement.To remove particles, hollow plugs should always be cleaned with a suitablecleaning liquid before using them for the first time (see Chapter 11.1 and p. 95).C72IB002EN-E 73

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference1. Take a clean syringe and a clean hollow needle.• Low viscous samples: Use a microliter glass syringe and a hollow needle 0.7 (gauge 22) with Luerfitting. Fix the hollow needle to the syringe tip.• Highly viscous samples: Use a 1 mL plastic syringe and a hollow needle 1.2 (gauge 18) with Luerfitting.NOTICEDo not use syringes that contain lubricants or rubber sealings. Thesematerials can dissolve into your sample and lead to a systematicmeasurement error.TIPClean the microliter glass syringe with a suitable cleaning liquid for your samplebefore using it for the first time. See Chapter 11.1 and Chapter 11.3.If the glass syringe is not dry after cleaning it, remove traces of the cleaning liquidby drawing in approx. 10 µL of sample and ejecting it into the waste vessel.2. Take the capillary and put a Filling Adapter Luer Female on its top end (nearthe twist protection). Press the adapter downwards and turn the capillary till itclicks into place.Fig. 9 - 10Mounting the Filling Adapter Luer FemaleThis adapter is required to have a hold on the capillary when inserting it intoor removing it from the capillary block.3. Fill the syringe with sample.• Low viscous samples: Slowly draw in the sample through the hollow needle. • Highly viscous samples: Proceed slowly in order not to generate air bubbles.Draw the sample into the syringe without attaching ahollow needle (the narrow channel might also generateair bubbles). Try to remove air bubbles from the syringe(turning it upside down). Wipe the tip of the syringe withtissue paper to remove sample residues. Fix the hollow needle and remove the air from it.Fig. 9 - 11Filling a microliter syringe with sample4. Hold the open end of the capillary upwards.74 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/ME5. Insert the hollow needle into the capillary as faras possible. It should touch the closed end.6. Slowly fill the sample by pushing the plungerdownwards.7. While the liquid rises, draw the hollow needleupwards. Make sure it remains below the liquidlevel and below any air bubbles.8. Fill sample until the liquid reaches the tip of thecapillary.Fig. 9 - 12 Filling the short capillary with amicroliter syringe9. Check the entire filling. The capillary must befilled without any bubbles or particles.• Turn the capillary by 360° around itslongitudinal axis and look it closely up anddown. Tilt it a bit if necessary.360°• If detecting a bubble (see arrow), wait till itrises to the top or remove it with the hollowneedle.Fig. 9 - 13Checking for and removing air bubbles• Highly viscous substances: Place the capillary into a stable, high vessel and wait till air bubbles rise tothe top and leave the capillary. Depending on the viscosity this may take upto 15 minutes or more. If required, fill some more sample and wait againuntil there are no bubbles left.10. Place a clean ball in the capillary. Do not touch the ball with bare fingers.• Possibility A: Place the funnel (part of thestandard supply) on top of the capillary anddrop the ball into the funnel. If using the balldispenser, press the release button once todrop a ball into the funnel.• Possibility B: Use the plastic tweezers toplace the ball directly in the capillary.ABFig. 9 - 14Inserting a ball with funnel (A) or directly (B)TIPIf using a factory adjustment ball (see Chapter 8.4), tip it directly from its containerinto the funnel.C72IB002EN-E 75

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME ReferenceIf the sample has got a high surface tension the ball may rest on the liquid surface.• Knock the funnel with your finger to make the ball drop into the sample.• Slowly remove the funnel in case the ball sticks to it.• If not using the funnel, push the ball into the capillary withthe tip of the hollow needle.Fig. 9 - 15Pushing the ball into the capillary• If small air bubbles are attached to the ball remove them with the hollowneedle.11. Check the filling again. The capillary must be filled without any bubbles orparticles.12. Select a suitable clean hollow plug for the capillary andclose it. Press the hollow plug firmly into the capillary.Make sure not to insert air bubbles with the plug (tilt theplug slightly when first inserting it). Turn the hollow plug to insert it deeper into the capillary. Ifnecessary, remove a little sample with the hollow needle toinsert the plug properly.The sample liquid should rise into the hollow plug, but asmall air reservoir inside the plug is necessary for pressureequilibrium. See Fig. 9 - 17.Fig. 9 - 16Inserting the hollow plug into the short capillary13. Wipe the capillary and plug on the outside to remove surplus sample. Cleanthe funnel (see Chapter 11.3).The capillary should now be filled air bubble free and closed tightly. Carefullycheck it for air bubbles again – they possibly stick to the ball or the plug. Checkthat the ball rolls freely in both directions. Air bubbles will slow down the ball andcause faulty measurement results.Fig. 9 - 17Filled short capillary with ball (see arrow) and hollow plugTIPNote down the serial number of the capillary. You need it when entering thecapillary data in the instrument’s "Capillary Administration".76 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/ME9.4 Inserting a Filled Capillary1. The Lovis capillary block should be in the manual-filling position (-45°). See Chapter 7.1.3.2. Screw the Screw Cap Back Side Closed (Fig. 9 - 20, 1) into the back end ofthe Lovis capillary block.3. Insert the filled capillary into the Lovis capillary block from the front end. Thetwist protection (Fig. 9 - 18, arrow) should point upwards. Fit it into thecorresponding recess (Fig. 9 - 19, arrow) by slightly rotating the capillary.Fig. 9 - 18 Capillary twist protection Fig. 9 - 19 Recess for twist protectionDue to the twist protection the capillary cannot turn sideways. The capillary is notheld in place by any O-rings, it sits loosely inside the Lovis capillary block.NOTICE• If the twist protection is not properly fitted into its recess, the capillary maypossibly break when fastening the Screw Cap Front Side Closed. Makesure the capillary is properly placed inside the Lovis capillary block.• The capillary is fixed inside the capillary block by the Screw Cap FrontSide Closed. It will fall out if turning the block and this cap is not mounted.4. Mount the Screw Cap Front Side Closed (2) on the Lovis capillary block.3Fig. 9 - 202Mounting the screw caps closed11 ... Screw Cap Back Side Closed2 ... Screw Cap Front Side Closed3 ... Metal ring of Screw Cap Front Side ClosedThe Lovis 2000 M/ME is now ready for measurement.C72IB002EN-E 77

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference9.5 Mounting a Capillary for Flow-Through FillingThis chapter describes how to assemble the capillary for flow-through filling.The required flow-through system consists of the capillary with the ball, two UNFadapters for connecting hoses and two open screw caps.Preparing and inserting the capillarySelect a capillary (see Chapter 9.1).1. The Lovis capillary block should be in the Flow-through filling position (+20°).See Chapter 7.1.3. In this position the front end is lower than its rear end.2. Take the capillary (1) and the Filling Adapter UNF (2). Plug the FillingAdapter UNF onto the end with the twist protection. Press the adapterdownwards and turn the capillary till it clicks into place.Fig. 9 - 21Mounting the Filling Adapter UNF on the front side of the capillaryTIPNote down the serial number of the capillary. You will need it later when enteringthe capillary data in the instrument’s "Capillary Administration".3. Hold the free end of the capillary upwards and place the funnel (part ofstandard delivery) on top of the capillary. Take a ball with the plastic tweezersand drop it into the funnel. Alternatively, place the ball dispenser on top of the capillary. Press therelease button once to drop a ball into the capillary. See Fig. 9 - 2, p. 70.TIPIf having ordered a factory adjustment, tip the adjustment ball directly from itscontainer into the funnel.4. Insert the empty capillary plus the ball (1) into the Lovis capillary block fromthe front end. The twist protection (Fig. 9 - 22, arrow) should point upwards.Fit it into the corresponding recess (Fig. 9 - 23, arrow) by slightly rotating thecapillary. Hold the capillary by the Filling Adapter UNF (2) on the front side.21Fig. 9 - 22 Capillary twist protection Fig. 9 - 23 Recess for twist protect.78 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/MENOTICEIf the twist protection is not properly fitted into its recess in the block, thecapillary may be damaged. Make sure the capillary is properly inserted intothe Lovis capillary block. Keep holding the capillary until it is fixed with theScrew Cap Front Side - Open, or it will fall out of the capillary block and break!25346Fig. 9 - 24Installing the flow-through system1 ... Capillary plus ball2 ... Filling Adapter UNF on the front side3 ... Screw Cap Front Side - Open4 ... Metal ring of Screw Cap Front Side - Open5 ... Filling Adapter UNF on the back side6 ... Screw Cap Back Side - Open5. Mount the Screw Cap Front Side - Open (3) on the Lovis capillary block.6. Plug a second Filling Adapter UNF (5) onto the back end of the capillary.7. Fasten the Screw Cap Back Side - Open (6) on the back side of the Loviscapillary block. Tighten both screw caps with your fingers.Fig. 9 - 25Flow-through system ready for hose connection, front and back side8. Next, connect the hoses. For filling with a syringe, see Chapter 9.6. For fillingwith an Xsample sample changer or for combinations with other measuringmodules, see SECTION II : Flow-Through Systems.C72IB002EN-E 79

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME Reference9.6 Hose Connections Flow-through FillingThis chapter separately describes how to connect the different hoses:• Lovis 2000 M – Filling Support to Lovis capillary block (p. 80)• Lovis 2000 ME + DMA M/DSA M – Cell outlet to Lovis capillary block (p. 80)• Lovis 2000 M/ME – Lovis capillary block to waste bottle (p. 82)Lovis 2000 M – Filling Support to Lovis capillary block1. Take an Adapter Luer 1/4" UNF (1 – Mat. No. 64792, part of the AccessoryKit Basic) and fasten it to the upper side of the Filling Support (2). Tighten itwith a 8 mm wrench (part of the Accessory Kit - Basic) to avoid accidentalloosening.2. Take the hose 350x3x2 FEP (3). Fasten one end to the Filling Support (2)from below. Bend the hose into a loop:TIPAvoid sharp bends or kinks!• Take the free end of the hose and bend itsideways towards the capillary block.Guide it counter-clockwise into a wide loop.• Fasten the free end to the Filling AdapterUNF (4) which projects from the Loviscapillary block.1 2 31 ... Adapter Luer 1/4" UNF2 ... Filling Support3 ... Hose 350x3x2 FEP4 ... Filling Adapter UNF (on front side)Fig. 9 - 26 Lovis 2000 M flow-through filling –Items listed below4Lovis 2000 ME + DMA M/DSA M – Cell outlet to Lovis capillary block1. Mount an Injection Adapter UNF 1/4" DMA or DSA (2a) to the outlet of theDMA M/DSA M. Refer to the DMA M/DSA M instruction manual for the correctprocedure.TIPIf your DMA M/DSA M has also got a Filling Support, you can fill the system witha syringe in vertical position. Connect the hoses as follows:80 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/ME• Fasten an Adapter Luer 1/4" UNF (1 – Mat. No.64792, part of the Accessory Kit - Basic) andfasten it to the upper side of the Filling Support (2).Tighten it with a 8 mm wrench (part of theAccessory Kit - Basic) to avoid accidentalloosening. • Fasten an Injection Adapter UNF 1/4" DMA or DSAto the DMA M/DSA M cell inlet (8) instead of theInjection Adapter Luer (see the respectiveinstruction manual).2178Fig. 9 - 27Hose for Filling Support – DSA 5000 M• Select a connection hose (7):DMA MHose 120x3x2 PTFE – suppliedwith the Filling support, Mat. No.106019 or 106021DSA 5000 MHose 220x3x2 FEP "CC" –Mat. No. 25540 (part of the LovisAccessory Kit - Complete)• Fasten one end of the hose (7) to the Filling Support from below and fastenthe other end to the Injection Adapter UNF 1/4".2. Take the hose 350x3x2 FEP (3). Fasten one end to the Injection AdapterUNF 1/4" DMA/DSA of the DMA M/DSA M (1a). To avoid that this hose getsentangled with the syringe, bend it into a loop:• Take the free end of the hose and bend it sideways towards the capillaryblock. Guide it counter-clockwise into a wide loop (steps I. to III.).• Fasten the free end to the Filling Adapter UNF (4) which projects from theLovis capillary block.1a1II.738I. III.4Fig. 9 - 283Hose DSA 5000 M to capillary block without / with Filling SupportFig. 9 - 27 and show the hose connections for a DSA 5000 M. Connect and bendthe hoses (3, 7) in the same way for a DMA M.1 ... Adapter Luer 1/4" UNF1a ...Injection Adapter UNF 1/4" DMA or DSA2 ... Filling Support3 ... Hose 350x3x2 FEP4 ... Filling Adapter UNF (on front side)5 ... Filling Adapter UNF (on back side)6 ... Waste hose 530x3x2 FEP7 ... Optional connection hose: Filling Support – DMA M/DSA M inlet8 ... DMA M/DSA M inletC72IB002EN-E 81

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME ReferenceTIPFasten the Adapter Luer 1/4" UNF and the hoseproperly inside the Filling Support to make sure theconnection is leak tight. The end of the hose mustpress against the adapter (see arrows). There is noadditional sealing inside the Filling Support.Fig. 9 - 29Connection inside the Filling SupportLovis 2000 M/ME – Lovis capillary block to waste bottleThe hose 530x3x2 FEP serves as connection to the waste bottle.CAUTIONA light-weight waste vessel (like the plastic vessel of the standard delivery)will move around at the end of the waste hose when the Lovis capillary blockturns. It could easily tilt and waste liquid would spill out.Use the waste bottle glass 1 L which is massive enough to preventundesirable movement and comes with a break protection.3. Fasten one end of the waste hose (6) to the Filling Adapter UNF (5) on theback side of the Lovis capillary block. Fasten the other end to the screw cap (9) of the waste bottle (10).5 ... Filling Adapter UNF (on back side)6 ... Waste hose 530x3x2 FEP9 ... Screw cap of waste bottle10 ... Waste bottle56 9Fig. 9 - 30Hose Lovis capillary blockto waste bottle104. Make sure all hose connectors are properly tightened (finger tight).• Switch off the instrument and slowly turn the capillary block from -90° to+90° angle by hand.• Check that the hoses do not get entangled or sharply bent.• Check that the hose connectors (especially the one at the DMA M/DSA Moutlet) do not become loose.The assembly is now ready for filling (see Chapter 9.7).82 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference9 Filling the Lovis 2000 M/ME9.7 Filling the Flow-Through Assembly Manuallywith a SyringeThis chapter describes how to fill a Lovis flow-through system with a syringe. It applies to• Lovis 2000 M• Lovis 2000 ME in combination with DMA M/DSA M.For filling with an Xsample sample changer or for combinations with othermeasuring modules, see SECTION II : Flow-Through Systems.TIPMake sure all parameters have been set according to your requirements beforeyou fill the capillary (see Chapter 10.1).Once filled, measurement should be started immediately, otherwise the ball willroll back to the lower end (see "Explanation", p. 84)!NOTICEDo not use syringes that contain lubricants or rubber sealings. Thesematerials can dissolve into your sample and lead to a systematicmeasurement error.1. Take a clean syringe (filling volume 10 or 20 mL) and draw in sample liquid.Proceed slowly and continuously to avoid the formation of air bubbles. Turnthe syringe upside down and remove remaining air bubbles by pressing theplunger upwards a bit (until a drop of liquid emerges from the tip).2. Connect the syringe to the instrument.Lovis 2000 M1Fig. 9 - 31Lovis 2000 M – Flow-through filling with a syringePlug the syringe firmly into the Adapter Luer (1) in the Filling Support.C72IB002EN-E 83

9 Filling the Lovis 2000 M/ME SECTION I : Lovis 2000 M/ME ReferenceLovis 2000 ME plus DMA M/DSA M8Fig. 9 - 32Lovis 2000 ME with DMA M – Flow-through filling with a syringePlug the syringe firmly into the Injection adapter Luer of the DMA M/DSA M(8; see also ).If using a Filling Support, plug the syringe firmly into the fixed Adapter Luer(1 – see Fig. 9 - 27 and ).Filling3. Push the plunger of the syringe slowly and continuously until liquid flowsfrom the end of the waste hose into the waste bottle. If using a DMA 4500/5000 M or DSA 5000 M the filling of the density measuring cell can beobserved via U-View TM (refer to the corresponding instruction manual).4. Start a measurement as soon as the measuring assembly is correctly filled!Explanation: If waiting to start measurement for longer than approx. 10 swith the Lovis capillary block in the Flow-through filling position, the ball willroll to the front end of the capillary. Depending on the sample’s viscosity theball might possibly not roll to the back end (starting position for correct balldetection) fast enough, when the measurement is finally started. In that casethe ball could not be detected.5. Leave the syringe in the filling position during the measurement.NOTICEThe waste hose is usually higher than the DMA M/DSA M inlet or the FillingSupport. The pressure of the liquid column in the hose will cause the liquid toflow out at the DMA M/DSA M inlet or at the Filling Support as soon as thesyringe is disconnected. Draw the sample liquid from the system back into thesyringe before removing the syringe!84 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference10 Performing a Measurement10 Performing a MeasurementThis chapter describes all necessary steps to obtain valid measurement results.Before starting a measurement a filled capillary must be placed inside the Loviscapillary block and all parameters should be set according to your requirements.10.1 Preparing a MeasurementSelect a capillary/ball configurationCapillary/ball configurations can be defined by an administrator or manager user(see Chapter 7.1.1). Persons with operator rights do not have access to theMeasuring System Settings!Any user can select a capillary/ball configuration from the existing list whenstarting measurement. See Chapter 10.2.Select a methodAny user can select an existing method (see Chapter 7.2) for measurement.1. Tap "Method". Select a measuring method from the list on the screen.2. Tap on the selected method and confirm with "OK".3. The instrument returns to the main screen and shows the display layoutassigned to the selected method.Enter the quick settings valuesThe values of all parameters stored in the quick settings of a method can bechanged by any operator.If a sample list has been defined, the Quick Settings button is replaced by the button.1. Tap "Quick Settings".2. Tap on a parameter to highlight it. Tap on the field in the "Parameter Select"column to alter the value or to select a different input from the drop-down list.3. Enter a value or make a selection and tap .4. Perform these steps for each quick settings parameter.5. Tap .The quick settings parameters set by default are:C72IB002EN-E 85

10 Performing a Measurement SECTION I : Lovis 2000 M/ME Reference• Sample name• Measurement Mode (see Chapter 7.2.3)• Density for Start Temperature• Density for End Temperature• Lovis CapillaryMandatory input parameters are marked as:TIP • If using the density values of a DMA M/DSA M you need not enter anydensity value.• If not performing a temperature scan or temperature table scan, only onedensity value needs to be entered ("Density for Start Temperature").• You can also enter the density value(s) as "Formula Parameters" in theMethod Settings". See Chapter 7.2.4.Once the user has altered the value of a quick settings parameter (the valuediffers from the one preset for this method), it is marked with a : *Before starting a measurement, check whether:• the hoses are connected correctly (if applicable)• the hose connections are tight (if applicable)• the waste hose leads into the waste container (if applicable)• the waste container is not full (if applicable)• the correct sample names are entered• the method settings are set correctly• suitable cleaning liquids are available.Fill the capillary or the measuring assembly (see Chapter 9).10.2 Start Measuring1. Tap .• The instrument displays all available capillary/ball configurations andprompts you to select the one actually used.• If the automatic capillary detection (see Chapter 7.1.2) is deactivated, nomessage or prompt is displayed. • In case only one capillary/ball configuration is stored in the instrument, itautomatically uses this one; no message or prompt is displayed.• In case you have not entered mandatory parameters (e.g. Lovis density orsample name) the instrument prompts you to do so.86 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference10 Performing a Measurement2. The instrument starts measuring. Wait till the measurement is finished.465321Fig. 10 - 1Method Lovis /DMA – Example: Measurement in progress• A red bar (1) shows the progress.• A message gives the sample name (2) and the measurement status (3):"Sample name: Measuring". If no sample name has been entered, themessage gives just the measurement status (3): "Measuring". • Depending on the selected method different data fields are displayed. • Available definite values (4) are displayed black.• Precalculated values (5) are gray.• Black dashes (6) – the instrument waits for a value or there is no valueavailable for this quantity (e.g. when doing Multi Concentrationmeasurements for polymer solutions, see Fig. 7 - 5, p. 47).C72IB002EN-E 87

10 Performing a Measurement SECTION I : Lovis 2000 M/ME Reference3. At the end of measurement an acoustic signal is given.8321Fig. 10 - 2Method Lovis detailed – Example: Measurement finished• The progress bar (1) turns green.• The message gives the sample name (2) and the measurement status (3):"Sample name: Finished". If no sample name has been entered, themessage gives just the measurement status (3): "Finished". • The measuring values are displayed.• The "Lovis Condition" output field shows the message "valid".• The quick access button to the diagnosis list (8) shows a green check forcorrect measurement.• The result values are frozen. The display can be unfrozen by tapping thebutton.• The result values are saved in the data memory and can be viewed,printed, exported or deleted.4. Measure the next sample or clean and dry the capillary and the densitymeasuring cell if applicable.Related topicsSafety Instructions: Chapter 2Density Values for Viscosity Calculation: Chapter 3.1.2Measuring System Settings: Chapter 7.1Lovis Measuring Methods Settings: Chapter 7.2Filling the Lovis 2000 M/ME: Chapter 9Cleaning and Storing the Instrument: Chapter 1188 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference10 Performing a Measurement10.3 Filling and Measurement Errors10.3.1 Status MessagesLovis conditionThe "Lovis Condition" output field gives information on the current status ofmeasurement or instrument and may show the following messages:temp. equilibrationmoving / inclining / turning back /startingCoil x forward/backward passedhomingvalidAppears during temperature equilibration. In thisstage no precalculated result can be determined bythe instrument.Appears when the Lovis capillary block moves duringmeasurement or waits for a run of the ball to start.Appears when the ball has passed the indicated coil(1 / 2 / 3) in the specified direction.Appears when the Lovis capillary block moves to theinitial position (manual filling or flow-through) at theend of measurement.Appears when the measuring temperature has beenreached and the measurement has been taken.Sample status iconsIn the first column of the sample list, you find an icon for each sample that hasalready been measured. The icon indicates the status of the sample:The sample was successfully measured.Reduced precision due to e.g. exceeding the set variationcoefficient, FW/BW-deviation, short runtime or a filling error.No result due to a malfunction (e.g. no ball detected).The measurement was aborted.C72IB002EN-E 89

11 Cleaning and Storing the Instrument SECTION I : Lovis 2000 M/ME Reference11 Cleaning and Storing the InstrumentTo assure constant and high accuracy of your measurements, use regular andeffective cleaning routines. Store the instrument as described in Chapter 11.7.Wipe sample spills on the instrument immediately with a soft tissue or dry cloth.Remove sample residues inside the Lovis capillary block or on the screw capsalso at once. Clean all these parts as described in Chapter 11.6.Capillaries should be cleaned and dried immediately after measurement.TIPIf measuring similar, fully miscible samples, one sample can be replaced by thenext. This requires more sample liquid, as the first sample batch must be flushedcompletely from the capillary by the following one. In this case always cleancapillaries at the end of a work shift, but at least once every 24 hours.Capillaries can be cleaned outside the Lovis capillary block or – if applying theflow-through method – assembled inside the Lovis capillary block.11.1 Selecting Cleaning LiquidsThe choice of the cleaning liquid(s) depends on the tested samples. All wettedparts of Lovis 2000 M/ME must be chemically resistant to the cleaning liquid(s).Refer to Appendix B and Appendix B.2 for an overview of concerned parts andmaterials and their chemical resistance against chemicals (see Appendix B.4)..CAUTIONCleaning liquids may involve health risks. Read Chapter 2.3 for your safety.• A suitable cleaning liquid must completely dissolve and remove the sample.It has to be a good solvent for all sample components. If this first cleaningliquid does not dry up fast and/or without leaving residues, a second cleaningliquid is required.• Cleaning liquid 2 must completely dissolve and remove cleaning liquid 1 andis easily evaporated by a stream of dry air.Recommended for aqueous samples and beverages: water (cleaning liquid 1)and ethanol (cleaning liquid 2).Recommended for polymer solutions: the polymer solvent which is contained inthe solution (cleaning liquid 1).Recommended for petrochemical samples: solvent naphtha (only one cleaningliquid).If you are not sure if a cleaning liquid is suitable for your sample, perform apreliminary test in a test vessel to see if any phase separation, precipitate oropalescence can be observed.90 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference11 Cleaning and Storing the Instrument11.2 Cleaning Standard Capillaries Outside theCapillary Block• If the sample liquid can be removed easily, just rinse the capillary withoutremoving the ball (see Chapter 11.2.1).• How to rinse flow-through systems is described in Chapter 11.5.• For a more thorough cleaning or to condition it, brush the capillary (seeChapter 11.2.2); this applies also to flow-through systems.CAUTIONRisk of burns if the Lovis 2000 M/ME is set to temperatures higher than 60 °C.Refer to the Safety Instructions, Chapter 2.11.2.1 Rinsing Standard CapillariesTaking apart – Capillary filled outside the Lovis capillary block1. Unscrew the Screw Cap Front Side Closed from the Lovis capillary block.2. Pull out the capillary by the Filling Adapter Luer Female.3. Hold the capillary with the tip upwards and remove the plug from the tip.4. To keep the ball in the capillary, fix the Filling and Cleaning Adapter LuerMale to the capillary tip.5. Turn the capillary around and hold it over a waste vessel.6. Remove the Luer plug from the Filling Adapter Luer Female.7. Plug a syringe filled with air into the Luer opening of the Filling Adapter LuerFemale and press down the plunger to remove the sample. Observe allsafety instructions applicable to this sample.Cleaning the capillary and filling accessories1. Plug a syringe filled with cleaning liquid into the Filling Adapter Luer Femaleand rinse the capillary. Move the plunger up and down to improve thecleaning effect. Rinse until no traces of sample are left.2. Wipe the capillary and the adapters on the outside.3. Wipe the filling accessories (plug or Luer plug, filling adapters; if requiredalso funnel or ball dispenser) with tissue paper. Clean these parts with somecleaning liquid.TIPIf cleaning the ball dispenser, do not take it apart! The built-in spring might get lost4. If necessary, repeat the above steps with a second cleaning liquid.C72IB002EN-E 91

11 Cleaning and Storing the Instrument SECTION I : Lovis 2000 M/ME Reference5. Wipe the plug and the Luer plug dry afterwards. Blow the adapters dry withclean compressed air or with the built-in air pump (see below instructions).Using the air pump1. Fix the Filling Adapter Luer Female (1) to thetop of the capillary (2). Plug the Adapter Luercone (3) of the silicone air hose into theadapter’s inlet.Start the pump with the buttontouch screen.on the312Fig. 11 - 1Drying a capillary with the air pump2. The drying time depends on the cleaning liquid, on the temperature and onthe ambient air humidity. The suitable drying time has to be determinedempirically. Tapto switch off the air pump.11.2.2 Brushing Standard Capillaries / ConditioningWhen to brush a capillary:• To condition a new capillary or to condition it after a special cleaning.• If rinsing alone or flow-through cleaning does not give satisfactory results.TIPFor brushing, the ball is removed from the capillary. To reuse the ball, carefullyempty the sample residues plus ball into a clean vessel first. To clean the ball seeChapter 11.4.Taking the assembly apart – Manual filling outside block1. Proceed as described in Chapter 11.2.1. Do not mount the Filling andCleaning Adapter Luer Male before emptying the capillary. Remove theFilling Adapter Luer Female.Taking the system apart – Flow-through filling method1. Make sure any remaining liquid has been evacuated from the system (drawthe liquid back into the syringe).2. The Lovis capillary block should be in position "Flow-through (hosesconnected)" (+20°). See Chapter 7.1.3.In this position the front end of the Lovis capillary block is lower than its backend and the ball will not fall out of the capillary when removing it.3. Unscrew both hoses from the capillary directly at the Lovis capillary block.4. Unscrew both screw caps from the Lovis capillary block and remove them.92 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference11 Cleaning and Storing the Instrument5. Hold the capillary at the Filling Adapter UNF on the front side. Pull thesecond Filling Adapter UNF off the back side of the capillary.6. Carefully pull the capillary from the Lovis capillary block by the FillingAdapter UNF. Take care that the capillary does not slip out and that the balldoes not drop out at the open end.7. Tip the ball into a small vessel.8. Pull the Filling Adapter UNF off the front side of the capillary.Brushing the capillary1. Rinse the inside of the capillary (1) with somecleaning liquid and brush it with the supplied brush(2). Move the brush repeatedly through the entirecapillary (see Fig. 11 - 2). Rinse the capillary again.2. Rinse the brush and wipe it with a lint-free cloth ortissue paper.3. If necessary, repeat these steps with a secondcleaning liquid.12Fig. 11 - 2Brushing a capillary4. Wipe the capillary on the outside.5. Clean the plugs and adapters and dry all items as described inChapter 11.2.1.TIPFor hard-to-remove samples, soak all items in some cleaning liquid first.If brushing does not yield satisfactory results, perform a special cleaning of thecapillary. See Chapter 12.1.11.3 Cleaning Short Capillaries11.3.1 Rinsing Short Capillaries and AccessoriesTaking apart1. Unscrew the Screw Cap Front Side Closed from the Lovis capillary block.2. Pull out the capillary by the Filling Adapter Luer Female.3. Hold the capillary with the tip upwards and remove the hollow plug from thetip.C72IB002EN-E 93

11 Cleaning and Storing the Instrument SECTION I : Lovis 2000 M/ME ReferenceRinsing the short capillary1. Empty the capillary. Observe all safety instructions applicable to this sample.• Low viscous samples:Turn the capillary around and hold it over a waste vessel.• Highly viscous samples:Draw the sample from the capillary with a syringe with a hollow needle. Theball remains inside the capillary and can only be removed later with thecleaning liquid.TIPTo reuse the ball, carefully empty the sample residues plus ball into a clean vesselfirst. To clean the ball see Chapter 11.4.2. Fill a clean syringe with a hollow needle with cleaning liquid. Insert the hollowneedle into the capillary as far as possible. Fill the capillary with cleaningliquid. It should not overflow.If necessary wait a bit for the cleaning liquid to mix with the sample.3. Move the plunger up and down rapidly to improve the cleaning effect. Drawthe cleaning liquid back into the syringe. Empty the syringe into a wastevessel.4. Repeat the two above steps two to three times until no traces of sample areleft in the capillary. If the ball is still inside, let it roll out and rinse the capillarywithout the ball once.5. If necessary, repeat the cleaning procedure with a second cleaning liquid.6. Wipe the capillary on the outside with some cleaning liquid and tissue paper.Drying the short capillary1. Plug a clean hollow needle 1.2 (gauge 18) onto the Adapter Luer cone of theair hose.Fig. 11 - 3Fixing a hollow needle to the Adapter Luer cone of the air hose2. Insert the hollow needle into the capillary as far as possible. Blow thecapillary dry with the built-in air pump:• Start the air pump with the button on the touch screen.Fig. 11 - 4Drying a short capillary with the air pump94 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference11 Cleaning and Storing the Instrument• The drying time depends on the cleaning liquid, on the temperature and onthe ambient air humidity. The suitable drying time has to be determinedempirically. Tap to switch off the air pump.TIPFor drying capillaries always use hollow needles 1.2 (gauge 18). The wider diameter speeds up the drying.Cleaning the hollow plug1. Put the hollow plug into some cleaning liquid.2. Fill a clean syringe with a hollow needle with cleaning liquid. Insert the hollowneedle into the plug and rinse the inside of the hollow plug.SuitablediametersHollow Plug 1.59 / 1.8 2.5Hollow Needle 0.7 (gauge 22) 1.2 (gauge 18)3. If necessary, repeat the above step with a second cleaning liquid.4. Take the hollow plug and wipe it with tissue paper.5. Blow the inside dry using a hollow needle and the built-in air pump (see"Drying the short capillary", p. 94).Cleaning the microliter glass syringe and hollow needleAlways clean the syringe and needles to avoid contaminating one sample withanother one.TIPHandle the microliter syringe with care to avoid damaging this precision tool. Pullthe plunger only as far out as the µL-scale. Do not use strongly alkaline cleaningliquids that will corrode the glass.1. Empty the syringe into a waste vessel.2. Draw in some cleaning liquid through the hollow needle and move theplunger up and down repeatedly. If necessary wait a bit for the cleaning liquidto mix with the sample. Empty the syringe into a waste vessel.3. Repeat the above step two to three times until no traces of sample are left.Wipe the hollow needle with tissue paper.4. If necessary, repeat the procedure with a second cleaning liquid.5. Remove the hollow needle.6. Pull the plunger up a bit and let the syringe air-dry.7. Dry the hollow needle with tissue paper. Blow it dry with the built-in air pump(see "Drying the short capillary", p. 94).C72IB002EN-E 95

11 Cleaning and Storing the Instrument SECTION I : Lovis 2000 M/ME ReferenceCleaning the funnel1. Wipe the funnel with some cleaning liquid and tissue paper.2. For hard to remove samples, soak it in some cleaning liquid and brush it.Give it a final rinse and dry it with tissue paper.11.3.2 Brushing Short Capillaries / ConditioningWhen to brush a short capillary:• To condition a new capillary or to condition it after a special cleaning.• If rinsing alone does not give satisfactory results.1. Fill some cleaning liquid into the capillary with a syringe with a hollow needle.2. Push a supplied brush as far as possible into the capillary and rotate it. Moveit up and down repeatedly.3. Pull the brush out again and rinse it with cleaning liquid. Rinse the capillaryas described above.4. If necessary, repeat the procedure.5. Dry the short capillary as described in Chapter 11.3.1.TIPFor hard-to-remove samples, soak all items in some cleaning liquid first.If brushing with the supplied brush leaves residues inside the dead end of thecapillary, perform a special brushing (Chapter 12.2) or perform a special cleaningof the capillary (see Chapter 12.1).11.4 Cleaning BallsTIPDo not handle balls with bare fingers! Finger prints influence the measurementresults. If using the plastic tweezers also clean them to avoid contaminating aclean ball with sample residues.1. Put the ball into some cleaning liquid.2. Put the ball onto a piece of tissue paper using the plastic tweezers.3. Fold a bit of tissue paper over the ball. Wet itwith some cleaning liquid. Rub the ball with thetissue paper and one finger.Wear protective gloves if required.Fig. 11 - 5Rubbing the ball with tissue paper4. Rinse the ball with some more cleaning liquid and let it dry on tissue paper.96 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference11 Cleaning and Storing the Instrument11.5 Manually Cleaning Capillaries with the Flow-Through MethodThis is a method for cleaning capillaries filled with the flow-through method (alsoin combination with DMA M/DSA M). It is not applicable to condition newcapillaries. For a more thorough cleaning take the assembly apart and clean allparts separately (see Chapter 11.2 – also for conditioning new capillaries).CAUTIONRisk of burns if the Lovis 2000 M/ME is set to temperatures higher than 60 °C.Refer to the Safety Instructions, Chapter 2.NOTICEMake sure any remaining liquid has been evacuated from the system. Drawthe liquid back into the syringe or the pressure from liquid column in the wastehose will lead to sample spilling out of the Filling Support (Lovis 2000 M) orthe DMA M/DSA M sample inlet.1. Select a suitable cleaning liquid as explained in Chapter 11.1.2. Fill a new 10 mL or 20 mL syringe with cleaning liquid and connect it to theAdapter Luer in the Filling Support or to the DMA M/DSA M inlet. Press theplunger into the syringe until the cleaning liquid flows into the waste bottle.3. Move the plunger backwards and forwards a few times to intensify thecleaning effect. Empty the syringe completely.NOTICEBefore disconnecting the syringe, draw the liquid remaining in the systemback into the syringe.4. Check the hoses for sample residues. They must be completely clean (novisible traces). If necessary, repeat the above cleaning steps.5. If required, rinse the entire system again with a second cleaning liquid (seeChapter 11.1). Proceed as described above.6. To dry the system, plug the Adapter Luer cone of the air hose into theAdapter Luer in the Filling Support or into the DMA M/DSA M inlet. Switch onthe air pump with the buttonon the touch screen.7. Select a drying time which is long enough to dry the entire system. Thedrying time depends on the cleaning liquid, on the temperature and on theambient air humidity. The suitable drying time has to be determinedempirically. Tapto switch off the air pump.C72IB002EN-E 97

11 Cleaning and Storing the Instrument SECTION I : Lovis 2000 M/ME Reference11.6 Cleaning Outer Instrument Parts11.6.1 Housing and Touch ScreenTo clean the instrument housing, the outside of the Lovis capillary block or theTouch screen, use a soft tissue that can be wetted with ethanol or warm water, ifnecessary with some mild cleaning agent (pH < 10) added.WARNINGEthanol is a highly flammable liquid.• Make sure that all safety instructions regarding the use of flammableliquids (see Chapter 2) are strictly followed.NOTICENever use:• highly nonpolar solvents (e.g. toluene, hexane, solvent naphtha)• strong acids or bases (e.g. nitric acid, sulfuric acid, hydrochloric acid,caustic soda)• abrasive cleaning agents (containing particles) or strong mechanicalaction (steel brush).To replace the protection foil of the display, refer to Appendix E.2 or to theinstruction manual of your DMA M/DSA M.11.6.2 Capillary Block and Screw CapsClean the cover of the Lovis capillary block as the housing (see Chapter 11.6.1).If there are sample residues inside the Lovis capillary block or on the screw caps,remove them at once.Clean the inside of the Lovis capillary block and the screw caps with cotton buds.For hard to remove residues use a supplied nylon brush. Select a cleaning liquidthat removes the sample and does not attack the material of the parts. Refer toChapter 11.1 "Selecting Cleaning Liquids" and Chapter B.2 "Parts Possibly inContact with Sample Liquid".11.7 Storing the InstrumentRemove the capillary from the Lovis 2000 M/ME. Empty and clean all capillaries.See Chapter 11.2, Chapter 11.3 and Chapter 11.5. Store all capillaries, fillingequipment and hoses in the case of the supplied Accessory Kit.Make sure no sample residues or spills remain on the instrument or inside theLovis capillary block. Clean these parts as described in Chapter 11.6.98 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference12 Customer Maintenance12 Customer MaintenanceTo keep your Lovis 2000 M/ME working smoothly and reliably, regularly performthe following checks and maintenance tasks. The required steps depend on theconfiguration of your measuring instrument.Filling outside the capillary block – Lovis 2000 M:What to do:Visual inspection of capillary and adapters.Special cleaning of the capillary.Replacing the O-rings of the capillary adapters.When:• Daily; before starting measurements.• After changing the capillary.• Once a year.• If cleaning outside the capillary block doesnot give satisfactory results.See Chapter 12.1.• If the capillary adapters are leaking or do nolonger hold tightly on the capillary.• If fixing Kalrez ® O-rings for better chemicalresistance. See Chapter 12.5.Flow-through filling – Lovis 2000 M or Lovis 2000 ME inside DMA M/DSA M:What to do:Visual inspection of capillaries and adapters.Visual inspection of hoses and hoseconnections.Tighten all hose connections.Leak test of all connected hoses.Special cleaning of the capillary.Regularly perform all maintenance stepsaccording to the schedule given for DMA M/DSA M, if any.Replacing the O-rings of the capillary adapters.When:If residues inside the capillary are suspectedthat cannot be removed by standard flowthroughcleaning. See Chapter 11.2 andChapter 12.1.• Daily; before starting measurements.• After changing the capillary.• Monthly.• After changing the capillary.• Monthly.• After hoses were dis- and reconnected.• If leakage is suspected.See Chapter 12.4.• Once a year.• If standard cleaning outside the capillaryblock does not give satisfactory results .See Chapter 12.1.Consult the instruction manual of your DMA M/DSA M model.• If the capillary adapters are leaking or do nolonger hold tightly on the capillary.• If fixing Kalrez ® O-rings for better chemicalresistance. See Chapter 12.5.C72IB002EN-E 99

12 Customer Maintenance SECTION I : Lovis 2000 M/ME Reference12.1 Special Cleaning of CapillariesBesides cleaning the capillary after each measurement or measurement cycle, aspecial cleaning of the glass should be performed regularly – at least once a year.The special cleaning procedure removes deposits, which have built up on theglass over time. Use e.g. hydrochloric acid 15 % or a suitable commerciallyavailable glassware cleaner.Take care when using strong alkaline cleaning agents as they damage the glass.Changes in the glass surface will influence the capillary adjustment. If you reallyneed to use a strong alkaline cleaning agent, verify the capillary adjustment witha substance of known viscosity before starting your next measurements.CAUTIONAggressive cleaning agents are a potential risk of injury. Skin contact maycause injury to bare hands. Splashes may cause injuries to the eyes.To avoid hurts, use appropriate means of personal protection like safetygoggles, gloves and apron.Prepare, use and dispose of the cleaners as indicated by the manufacturer.Follow all safety instructions given in the safety data sheet of the product.Cleaning procedure1. Place the capillary into the cleaning liquid to soak it. The duration dependson the cleaning liquid, the cleaning temperature and on the deposits on thecapillary. Brush the capillary with the supplied brush to achieve a bettercleaning of the inside of the capillary. For short capillaries perform anadditional special brushing (see Chapter 12.2).2. Rinse the capillary efficiently with deionized water, then rinse with a volatilesolvent for quick drying. Dry the capillary by blowing through with cleancompressed air or with the built-in air pump.3. Before using the capillary again, brush and rinse it with a cleaning liquidsuitable for your application to condition the glass surface (seeChapter 11.2.2 or Chapter 11.3.2). This yields better measurement resultsdue to better wetting of the glass surface.Examples for commercially available cleaning agents• Neodisher® cleaning agents. E.g. type LM3 is recommended from Schottalso for viscometer cleaning. See the Duran-Group website for furthersuitable cleaners.• Chromium-free cleaning agents like Nochromix ® . This is a substitute forchromic acid. See the Godax website.• Deconex ® laboratory glass cleaners. For a suitable cleaner see the Borerwebsite.TIPInstead of manual cleaning and brushing you can use an ultrasonic bath.100 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference12 Customer Maintenance12.2 Special Brushing of Short CapillariesThe brushes supplied with the Lovis 2000 M/ME serve to clean the inner walls ofa short capillary but have no effect on the dead end (no bristles on the wire tip).Remove sample residues inside the dead end of a short capillary as follows:1. Take a commercial interdental brush and insert it (wire end first) into the tip ofa hollow needle 1.2.Fig. 12 - 1 Interdental brush / inserting it into the hollow needle 1.22. Push the brush into the hollow needle 1.2 until it is no longer visible. (Youcan use the hollow needle 0.7).Fig. 12 - 2 Pushing the interdental brush into the hollow needle 1.23. Fill some cleaning liquid into the short capillary.4. Insert the hollow needle 1.2 containing the brush into the short capillary asfar as possible.5. Insert a hollow needle 0.7 into the Luer end of the hollow needle 1.2 andpush the brush out of the needle for about 4 mm.approx. 4 mmFig. 12 - 3Pushing the interdental brush into the dead end of the capillary6. Remove the hollow needle 0.7.Now the bristles of the interdental brush are bent into the dead end of thecapillary.7. Clean the dead end. Turn the hollow needle 1.2 with the brush around insidethe capillary, move it up and down a bit.8. Remove the hollow needle 1.2 (plus brush) from the short capillary.9. Rinse the capillary with fresh cleaning liquid. If necessary repeat theprocedure until all sample residues in the dead end are removed.10. Dry the capillary as described in Chapter 11.3.1.C72IB002EN-E 101

12 Customer Maintenance SECTION I : Lovis 2000 M/ME Reference12.3 Inspection of Hoses and Hose ConnectorsTo meet safety requirements and for proper function of the system, a regularinspection is required.1. Check all hoses and hose connectors of the measuring assembly at leastmonthly if they are connected properly. If required, tighten the connectors.2. Check the color of the FEP hoses. Replace very dark or turbid hoses.3. Check all hoses for mechanical damage (e.g. kinks). Replace damagedhoses immediately.12.4 Leak Test of all Connected HosesThe leak test works by feeding air into the system while simultaneously closingthe waste hose. This way air pressure builds up inside. Leakage can be detectedby a hissing noise from the leaky part.1. Tap to switch on the air pump. Plug the Adapter Luer cone of the airhose into the DMA M/DSA M inlet or – if not using a DMA M/DSA M – intothe Adapter Luer in the Filling Support of the Lovis 2000 M.2. Screw an adapter UNF/UNF (optional accessory, see Lovis 2000 M/MEProduct Description List) onto that end of the waste hose that is connected tothe waste bottle during operation. Screw an adapter Luer 1/4" UNF (Lovisaccessories) into this adapter. Close the adapter Luer 1/4" UNF tightly with aLuer plug (Lovis accessories).The air flows into the DMA M/DSA M measuring cell – if applicable – and thenthrough the Lovis capillary into the waste hose.3. Listen to the noise of the air pump. Make sure that the noise is not drownedout by ambient noise.• The noise disappears: The system is leak tight.• The noise does not disappear, you may hear also a hissing noise: Try tolocate the leakage.4. When ready, remove the air hose from the Filling Support or from theDMA M/DSA M inlet. Tapto switch off the air pump. Reconnect thewaste hose to the waste bottle.102 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference12 Customer MaintenanceReasons for leakiness• A hose connector may be loose - check and tighten all hose connectors.Repeat the leak test.• One of the hoses is damaged - check all hoses and replace the damagedone. Repeat the leak test.• The O-rings of the filling adapters for the Lovis capillary may be damaged.Disassemble and check the parts. If required, replace the O-rings accordingto Chapter 12.5. Repeat the leak test.• The injection adapters of the DMA M/DSA M are loose or damaged (if one ofthese models is in use). Find solutions in the instruction manual of yourDMA M/DSA M.12.5 Replacing the O-Rings of the Filling AdaptersThis procedure is applicable also for changing the standard O-rings to Kalrez ®Spectrum 6375 (refer to the Lovis 2000 M/ME Product Description List on thesupplied USB flash drive).TIPAlways replace both O-Rings of a filling adapter!Required tools and spare parts• Hook for O-ring removal (supplied with the Accessory kit basic - Lovis)• O-Ring 5x1.5 FPM Viton ® Extreme, 2 pcs. per adapter (Pack of 10 pcs.supplied with the Lovis accessories; Mat. No. 100147)Removing the old O-rings1. To pull the O-rings from the filling adapter (2), place the hook (3) below one O-ring (1), turn it as shown in Fig. 12 - 4 (using it as a lever) and pull the O-ring from the groove. It is not important which one is removed first.3112Fig. 12 - 4Removing the O-rings from a filling adapter2. Clean the filling adapter with suitable solvent and dry it. See alsoChapter 11.2.C72IB002EN-E 103

12 Customer Maintenance SECTION I : Lovis 2000 M/ME ReferenceAttaching new O-ringsTIPAttach first the lower, then the upper O-ring.1. Put an O-ring into the filling adapter.2. Use the hook for O-ring removal to push the O-ring into the lower groove.First, place one section of the O-ring into its groove. Then, slowly move thehook around the inner wall of the filling adapter pushing the O-ring bit by bitinto place.3. Repeat the procedure for the upper O-ring.104 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference13 Service Utilities13 Service UtilitiesThis chapter describes Lovis-specific utilities. General utilities find in theInstrument Firmware Manual.13.1 Viewing and Printing Live Raw DataIf the monitor mode is active, you get a live view of sensor signals including rawdata in this menu. You can export or print the live raw data.After a measurement is finished, the live raw data of the main measuring unit arefrozen as well as the quantities on the main screen. To unfreeze the values, tapthe button.To view live raw data• Tap and select "Service > Live Raw Data".• Select "Lovis", "Calculations", "DCB - Air Pump", "DCB -Environment", or "DCB - CAN Connector".LovisYou can view a list of all data of the latest measurement. This includes set values,output values as well as state values (e.g. "valid").CalculationsYou can view a list of the calculations of the currently available output quantities.DCB - Air PumpAir Pump State Status of the air pump (0 or 1).DMA DCB - EnvironmentHeatsink temperatureRoom temperatureNTC temperatureTemperature of the heat sink.Temperature measured within the instrument.Temperature measured within the instrument.DCB - CAN ConnectorCAN Connector CurrentCurrent measured at the CAN interface [A].C72IB002EN-E 105

13 Service Utilities SECTION I : Lovis 2000 M/ME ReferenceTo print live raw data1. Tap , select "Service > Live Raw Data" and choose one of thegroups of live raw data quantities.2. To perform a printout on paper or to a PDF file, tap andfollow the instructions on the screen.13.2 Lovis UtilitiesThe Lovis utilities comprise functions which help to do maintenance or service onLovis 2000 M/ME.To perform an Initialization1. Tap "Menu > Lovis Utilities > Initialization".The Lovis capillary block first moves to -90°, then to 0° and stops in theselected home position (manual filling/ -45° or flow-through filling/+20°).A red growing bar and messages on the screen indicate the procedure’sprogress.2. If required, tap or quit with .To perform a Fan Service1. Tap "Menu > Lovis Utilities > Fan Service".2. Tap to start the fan in the Lovis capillary block and to stop this fan again.TIPIf the instrument is set to a measuring temperature < 20 °C, the fan in the Loviscapillary block must work to ensure proper cooling of the block. If the fan wasstopped the block would overheat and be damaged. Therefore the button is inactive for measuring temperatures < 20 °C.106 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference14 Firmware Update14 Firmware UpdateThe instrument firmware comes as a complete package including firmware for allmaster instruments and modules of a measuring assembly. The latest firmwarepackage at delivery time is supplied on a USB flash drive with the Lovis 2000 M/ME.NOTICEThe firmware can only be updated to a newer version. Down dating to anolder version is not possible!14.1 Backing up the Instrument SettingsYou can use this utility for:• Saving the current instrument status including all method settings and yourfavorites list for safety reasons for the case that someone changes thesettings accidentally.• Saving the current settings before you perform a instrument firmwareupgrade.• Copying the instrument settings to a further Lovis 2000 M or DMA M/DSA M.A backup file contains the following instrument settings and instrumentinformation:• Global settings in the control panel (regional settings, keyboard settings,network settings)• Method settings (display layout and content, result output settings, limitsettings, module settings, check definitions)• User functions• Users• System settings (instrument settings, global module settings, sample listsettings, audit trail settings, printout settings)3. Adjustments (all adjustment data)To make a backup of instrument settings1. Tap and select "Service > Backup Instrument Settings" to openthe "Backup" dialog.2. Select the data storage location (USB flash drive) and enter a "file name" oraccept the default file name.C72IB002EN-E 107

14 Firmware Update SECTION I : Lovis 2000 M/ME Reference3. Tap .4. Select the content of your backup file in the column "Value" and tap .5. Tap to create your backup file.The backup file is stored into the root directory of your USB flash drive.14.2 Restoring the Instrument SettingsNOTICEWhen restoring the instrument settings, make sure that the backup filecorresponds with the instrument type.1. Tap and select "Service > Restore Instrument Settings" to openthe "Restore" dialog.2. Tap to scan the content of the selected backup file.3. Select the location of your backup (USB flash drive) and the "file name"using the two drop-down boxes.The backup file must be stored in the root directory of your USB flash drive.4. Tap .5. Select the settings to be restored to your instrument in the column "Value"and tap .6. Tap to restore the settings.7. Follow the instructions on the screen.14.3 Checking the Firmware VersionThe currently installed firmware version can be seen in the System Information.In this menu, you find information about the configuration of the masterinstrument’s hardware and of the module(s), if connected. The instrument type,serial number and firmware versions of master and modules are shown in a table.Tap and select "Service > System Information" to open theconfiguration overview table.Use the vertical scroll bar to scroll through the table.Check the instrument firmware version and compare it with the version number ofthe supplied instrument firmware on the USB flash drive. If the version numberdisplayed on the instrument is older, you should perform a firmware update. For further information see the Instrument Firmware Manual Platform M.108 C72IB002EN-E

SECTION I : Lovis 2000 M/ME Reference14 Firmware Update14.4 Updating the Instrument FirmwareUpdating the instrument firmwareThe instrument firmware is the complete firmware package for the instrument,containing the module firmware, language packs and instrument firmware for allmaster instruments and modules of the measuring assembly. Anyway, modulefirmware or language packs can also be updated separately, if new versions areavailable and the other parts of the instrument firmware do not change.NOTICETo update the instrument firmware, you need administrator rights withactivated auto logon function or the administrator must log on and perform thefirmware update.TIPBefore starting the system update, export or print relevant measurement data andcreate a backup of the instrument settings (see Chapter 14.1).To update the instrument firmware1. Load the latest firmware package into the root directory of your USB flashdrive.2. Insert the USB flash drive into a USB interface of your instrument.3. Tap and select "Service > Update > System Update".4. Select the location (USB flash drive) and the "file name" of the new firmware.5. Tap .6. Follow the instructions on the screen.7. Restore the instrument settings (see Chapter 14.2), if required.Updating the module firmwareConsult the instruction manual of your master instrument respectively theInstrument Firmware Manual Platform M.Installing a language packConsult the instruction manual of your master instrument respectively theInstrument Firmware Manual Platform M.C72IB002EN-E 109

SECTION II : Flow-Through SystemsSECTION II :Flow-Through SystemsThis section describes setting up the hardware for all possible measuringassemblies including a Lovis 2000 M or a DMA M/DSA M as master instrumentcombined with different modules:• installing master instrument and modules• connecting the hoses for pressure filling and suction filling incl. chemicallyresistant hoses• putting the assembly into operationand more.110 C72IB002EN-E

SECTION II : Flow-Through Systems15 Overview15 OverviewLovis 2000 M/ME is a microviscometer based on the Rolling Ball Principleexplained in SECTION I - Chapter 3.2. It can be combined with DMA M/DSA M,several sample changers from the Xsample family and other modules. Fig. 15 - 1 Lovis 2000 ME + DMA M + Xsample 122Supported combinations• Lovis 2000 M with sample changers Xsample 122/22, Xsample 352/452 andXsample 52, see Chapter 18.• With DMA M series or DSA 5000 M, Lovis 2000 ME and sample changersXsample 122/22, Xsample 352/452 and Xsample 52, see Chapter 19. Forassemblies with DSA 5000 M only Xsample 122/22 are supported.• With DMA M, Lovis 2000 M/ME, Alcolyzer ME, HazeQC ME, pH ME andpossible subsets of these instruments see Chapter 20.• With DMA M/DSA M, Lovis 2000 ME, pH ME and possible subsets of theseinstruments see Chapter 21.• With DMA M/DSA M, Lovis 2000 M/ME, Abbemat see Chapter 22.C72IB002EN-E 111

15 Overview SECTION II : Flow-Through SystemsDefinition of master instrument and moduleA measuring assembly consists of the master instrument and one or moremodules. The master instrument always controls the connected modules.The Lovis 2000 M is the master if there is no DMA M/DSA M included.Whenever a DMA M / DSA M is included in the measuring assembly, the densitymeter or density and sound velocity meter is the master.112 C72IB002EN-E

SECTION II : Flow-Through Systems16 Checking the Supplied Parts16 Checking the Supplied PartsLovis 2000 M/ME was tested and packed carefully before shipment. However,damage may occur during transport.1. Keep the packaging material (box, foam piece, transport protection) forpossible returns and further questions from the transport and insurancecompany.2. Check the delivery for completion by comparing the supplied parts to thosenoted in the tables below and in your delivery list.3. If a part is missing, contact your Anton Paar representative.4. If a part is damaged, contact the transport company and your Anton Paarrepresentative.16.1 Supplied Parts Lovis 2000 ME16.1.1 Lovis 2000 ME MicroviscometerTable 16.1: Supplied Parts Lovis 2000 MESymbol Pcs. Article Description Mat. No.1 Lovis 2000 ME Microviscometer Module 9814322Fixing screws M4x20, A4Toothed contact washer M4(To be found in a small bag fixed to the Lovis 2000 MEhousing)(19751) a (61483) a1 Accessory Kit Basic-LovisAccessory Kit Complete-Lovis100219100220Type of accessory kit and contents depend on yourorder.1 Instruction Manuala. Material numbers in parentheses cannot be ordered separately. Available only upon request. Please contact your AntonPaar distributor.C72IB002EN-E 113

16 Checking the Supplied Parts SECTION II : Flow-Through Systems16.1.2 Lovis 2000 M Base UnitSupplied, if the assembly includes no DMA M / DSA M.Table 16.2: Supplied Parts Base UnitSymbol Pcs. Article Description Mat. No.1 Lovis 2000 M Base Unit 944621 Pack Protection foils for touch-screen(1 pack = 3 pcs.)814021 Waste bottle SVM 3000 0.5 L 178221 Power cord (country specific)EUR:GBR:USA:PRC:THAI:651466186552656270117973016.1.3 Module Housing Lovis METable 16.3: Supplied Parts Module Housing Lovis MESymbol Pcs. Article Description Mat. No.1 Module Housing Lovis ME 98309114 C72IB002EN-E

SECTION II : Flow-Through Systems16 Checking the Supplied PartsTable 16.3: Supplied Parts Module Housing Lovis MESymbol Pcs. Article Description Mat. No.1 CAN interface cable 0.6m 846651 CAN/Devicenet terminating resistor 839991 Crush protection Lovis on Xsample protectivecover1008713 Flathead screws M4x8(To fix the crush protection on the safety coverXsample.)3 Distance washer 5.6x8x0.5(For levelling the module housing ME on themaster instrument)1 Angular screw driver Torx T10(for the screws of the safety cover/crushprotection)1 Edge protection, PE, l = 177 mm(84162) a7423621304(1968) a(For the hose slit in the metal part of the Xsamplesafety cover.)a. Material numbers in parentheses cannot be ordered separately. Available only upon request. Please contact your AntonPaar distributor.16.1.4 Accessory KitsGenerally, the Lovis 2000 M/ME comes with the Accessory Kit Basic Mat. No.100219, basic content see Table 16.5. Depending on your order, additionalaccessories may be delivered. The following chapters show the contents of boththe basic and the optional complete kit.C72IB002EN-E 115

16 Checking the Supplied Parts SECTION II : Flow-Through Systems16.1.4.1 Accessory Kit Complete - LovisThis kit contains all relevant parts foroperating Lovis 2000 M/ME stand alone andas a part of various measuring assemblies.The capillary/ball equipment allowsmeasurements over the complete viscosityrange of the instrument.Fig. 16 - 1Accessory Kit Complete - LovisTable 16.4: Supplied Parts Accessory Kit Complete - Lovis Mat. No. 100220Symbol Pcs. Article Description Mat. No.1 Screw Cap Back Side - Closed 981751 Screw Cap Front Side - Closed 981711 Screw Cap Back Side- Open 981771 Screw Cap Front Side - Open 981661 set O-Ring 5x1.5 FPM 75 Viton® Extreme (10 pcs.)1001475 Syringe 1 mL Luer This item can be ordered in packs of 100 pcs. bythe following material number2 Syringe 10 mL Luer This item can be ordered in packs of 100 pcs. bythe following material number---101523---6442116 C72IB002EN-E

SECTION II : Flow-Through Systems16 Checking the Supplied PartsTable 16.4: Supplied Parts Accessory Kit Complete - Lovis Mat. No. 100220Symbol Pcs. Article Description Mat. No.1 USB flash drive 2 GB 870931 Luer Plug with Bore (10 pcs.) 998491 Luer Plug w/o Bore (10 pcs.) 998501 Filling Adapter Luer Female 981721 Filling and Cleaning Adapter Luer Male 986241 Filling Adapter UNF 981741 Capillary Lovis 1.59 mm (uncalibrated) 930951 Plug 1.59 mm (20 pcs.) 998431 Capillary Lovis 1.8 mm (uncalibrated) 930961 Plug 1.8 mm (20 pcs.) 99844Capillary Lovis 2.5 mm (uncalibrated) 93097Plug 2.5 mm (20 pcs.) 998451 Funnel for 1.5 mm ball - Lovis 870891 Ball dispenser 1.5 mm(Fig. shows dispenser incl. attached vial withballs)98922C72IB002EN-E 117

16 Checking the Supplied Parts SECTION II : Flow-Through SystemsTable 16.4: Supplied Parts Accessory Kit Complete - Lovis Mat. No. 100220Symbol Pcs. Article Description Mat. No.1 Set of balls 1.5 mm (300 pcs.) 731091 Set of balls 1.5 mm 4µ gold (300 pcs.) 206592 Adapter Luer cone 638632 Adapter Luer 1/4" UNF 647922 Adapter UNF/UNF 647902 Adapter UNF/screwed tube 188072 Adapter UNF/Hose (Female/Male) 267391 Injection Adapter UNF 1/4" DSA 122142 Injection Adapter UNF 1/4" 122192 Hose 220x3x2 FEP 2x1/4"-28 UNF "CC" 25540C118 C72IB002EN-E

SECTION II : Flow-Through Systems16 Checking the Supplied PartsTable 16.4: Supplied Parts Accessory Kit Complete - Lovis Mat. No. 100220Symbol Pcs. Article Description Mat. No.1 Hose 300x3x2 FEP 1x1/4"-28 UNF "A" 1090472 Hose 350x3x2 FEP 2x1/4"-28 UNF 255252 Hose 530x3x2 FEP 2x1/4"-28 UNF "C" 25523c2 Hose 600x3x2 FEP 2x1/4"-28 UNF 255241 Hose 650x3x2 FEP 1x1/4"-28 UNF "AD" 24809AD1 Tube d=2.1 D=2.4 L=20; 1.4571 679821.2 m Hose 1.6x3.2 Tygon 675400.6 m Hose 2x4 Viton® 546280.6 m Hose 2x4 Silicone 512731.2 m Hose 3x5 Silicone 508148 Cable clip self-adhesive L20 B15 H4 gr 620721 Brush 170x25x2.7 Nylon 0.06 (5 pcs.) 997781 Hook for O-ring removal 24951C72IB002EN-E 119

16 Checking the Supplied Parts SECTION II : Flow-Through SystemsTable 16.4: Supplied Parts Accessory Kit Complete - Lovis Mat. No. 100220Symbol Pcs. Article Description Mat. No.1 Plastic tweezers 631321 Angular screwdriver Torx T20(To mount the Lovis 2000 ME module into themaster instrument or housing)1 Open wrench 8 mm154623635(For tightening hose connections)2 Fuse 5x20 5A slow ceramics 276561 Sticker "Made with Viton® Extreme" 14193For the parts supplied with DMA M series, DSA 5000 M, Xsample samplechangers and other modules see the respective instruction manuals and deliverylists.16.1.4.2 Accessory Kit Basic - LovisThis kit contains only the standard partsrequired in any case for manualmeasurement.It is standard delivery for any Lovis.Fig. 16 - 2Accessory Kit Basic - LovisTable 16.5: Supplied Parts Lovis Accessory Kit Basic Mat. No. 100219Symbol Pcs. Article Description Mat. No.1 Brush 170x25x2.7 Nylon 0.06 (5 pcs.) 997781 Hook for O-ring removal 24951120 C72IB002EN-E

SECTION II : Flow-Through Systems16 Checking the Supplied PartsSymbol Pcs. Article Description Mat. No.1 O-Ring 5x1.5 FPM 75 Viton® Extreme (10 pcs.)1001475 Syringe 1 mL Luer This item can be ordered in packs of 100 pcs. bythe following material number2 Syringe 10 mL Luer This item can be ordered in packs of 100 pcs. bythe following material number---101523---64421 Plastic tweezers 631321 Angular screwdriver Torx T20 154621 Open wrench 8 mm3635(For tightening hose connections)1.2 m Hose 3x5 mm Silicone 508141 USB flash drive 2 GB 870932 Adapter Luer cone 638632 Adapter Luer 1/4" UNF 647928 Cable clip self-adhesive L20 B15 H4 gr 620721 Funnel for 1.5 mm ball - Lovis 870892 Fuse 5x20 5A SLOW ceramics 27656C72IB002EN-E 121

16 Checking the Supplied Parts SECTION II : Flow-Through SystemsSymbol Pcs. Article Description Mat. No.1 Sticker "Made with Viton® Extreme" 1419316.2 OptionsThis section lists only the most common options, which are described in thefollowing chapters during setting up several measuring assemblies.For all available options refer to the product description list (PDL) on the suppliedUSB flash drive.16.2.1 Drip PlateThis item is a required whenever a sample changer is part of the measuringassembly and a measuring module is placed on top of the master instrument. Ifthe Stand for Measuring Assembly (Mat. No. 105451) is used for larger setups,the drip plate is obsolete. The drip plate will avoid damages to the masterinstrument in the unlikely event of a leakage. See also Chapter 17.3.1 Drip Plate99384To put on top of the master instrument. Packageincludes a waste hose with nozzle and hoseclamp. The supplied fixing bracket with knurledscrews is not applicable for the measuringassemblies described in this manual.16.2.2 Waste BottleThis glass bottle with GL45 thread allows to connect the waste hose directly to itsscrew cap. It is mainly designed for use with the flow-through filling set or forXsample 22.For Xsample 122 the volume of the bottle is too low if all vials are filled to the rim.122 C72IB002EN-E

SECTION II : Flow-Through Systems16 Checking the Supplied PartsSymbol Pcs. Article Description Mat. No.1 Waste Bottle 1 L glass101655Borosilicate glass 3.3, equipped with screw capand insert with three 1/4"-28 UNF female threadsfor connecting the waste hose, PTFE gasket andprotective netting.1 Sealing gasket PTFE GL 45 (To order as spare part; this part applies also forthe optional waste container 8 L HDPE.)97027Replacement according to Chapter 17.4. It is thesame procedure as for the waste container8 L HDPE.16.2.3 Chemical Resistant Hose SetsThese kits can be used for measuring assemblies including an Xsample 122/22with or without DMA M/DSA M.Symbol Pcs. Article Description Mat. No.Contents according to theXsample 122 ProductDescription ListContents according to theXsample 122 ProductDescription ListContents according to theXsample 22/52 ProductDescription List1 kit Retrofit kit for Xsample 122 ChemicalContains a Hastelloy needle, FEP hoses,chemical resistant pump hose "Norprenechemical" with suitable cartridge for the pump, amagazine for 12 mL vials incl. 1 pack of 12 mLglass vials, further all required clamps,connectors and tools. Especially for use with awide range of chemicals.1 kit Retrofit kit for Xsample 122 PetroThis set is prepared especially for use withpetrochemical samples. It contains the sameparts as the retrofit kit for Xsample 122Chemical, except the pump hose. This is a"Norprene A-60-G" hose.1 kit Retrofit kit for Xsample 22 ChemicalContains FEP hoses, a chemical resistant pumphose "Norprene chemical" with suitable cartridgefor the pump, all required clamps, connectorsand tools. Especially for use with a wide range ofchemicals.968789688096879C72IB002EN-E 123

16 Checking the Supplied Parts SECTION II : Flow-Through SystemsSymbol Pcs. Article Description Mat. No.Contents according to theXsample 22/52 ProductDescription List1 kit Retrofit kit for Xsample 22 PetroThis kit is prepared especially for use withpetrochemical samples. It contains the sameparts as the retrofit kit for Xsample 22 Chemical,except the pump hose. This is a "Norprene A-60-G" hose.1 O-Ring 5x1.5 Kalrez® Spectrum 6375Serves best chemical resistance against nearlyall solvents, most acids and bases.For the filling adapters of a Lovis 2000 ME youneed 4 pcs.1 Sticker "Made with Kalrez®"This sticker is delivered automatically whenordering the above Kalrez O-rings.Put it on your device to avoid that O-rings areexchanged to a less resistant quality duringmaintenance or service.9688110151910192716.2.4 Low Volume OptionsFor Xsample 352 and 452 several accessories are available to save samplevolume. Installation see Chapter 18.1 or Chapter 19.1. In combination withAbbemat the low volume hoses are obligatory, see Chapter 22.7.Symbol Pcs. Article Description Mat. No.1 Magazine for 6 mL vials 48 positions100996Not suitable for Xsample 352.1 pack Vial 6 mL polystyrene(1 pack = 1500 pcs.)For Xsample 352 on request.1 Hose 300x3x1 FEP 2x1/4"-28 UNF (low volume)10139921875B(replaces Mat. No. 3443)1 Hose 530x3x1 FEP 2x1/4"-28 UNF (low volume)75860A(replaces Mat. No. 25523)1 Hose 600x3x1 FEP 2x1/4"-28 UNF (low volume)21874B(replaces Mat. No. 25524)124 C72IB002EN-E

SECTION II : Flow-Through Systems16 Checking the Supplied Parts16.2.5 Stands and Waste ContainersSymbol Pcs. Article Description Mat. No.1 Stand Measuring Assembly 750x380x280 105451This stand allows to place several modules ontoa master instrument in horizontal setup. Underthe stand, a waste container can be placed.(Stand comes without instrument.)1 Waste Container HDPE 8 LThis waste container can be placed under theStand Measuring Assembly. The combination ofstand and waste container is an easy solution tocollect non-hazardous waste liquids. Applies foruse with Xsample 122/22. For details seeChapter 17.4.1 Stand for XsampleThis stand lifts the position of the instrument byapprox. 200 mm. Under the instrument, theoptionally available safety waste container canbe placed. This combination serves a safe andeasy solution for collecting hazardous wasteliquids. Applies for use with Xsample 352/452.1 Safety Waste Container1059798758487585The safety waste container "Asecos" is made ofstainless steel and provides a volume of 5 L,flame arrester, venting screw, connector forground cable and dosage tap.C72IB002EN-E 125

17 Preparing Module(s) and Master Instrument SECTION II : Flow-Through Systems17 Preparing Module(s) and MasterInstrument17.1 Steps to Perform the Hardware Setup1. Check the mains supply according to the specifications. See Appendix A.2. Prepare enough space on a flat lab bench. Pay attention to the requirementsfor the setup location according to Chapter 6.1.1. When retrofitting an existing density meter/sound velocity meter: Operatingthis instrument together with a Lovis 2000 ME and other modules requires atleast instrument firmware version 2.30 or higher. Perform the firmwareupdate of the master instrument before setting up the measuring assembly’shardware. The latest version you will find on the USB flash drive suppliedwith Lovis 2000 M/ME. For the firmware update see Chapter 14 or consultthe DMA M/DSA M instruction manual.NOTICEUpdate DMA M/DSA M to the instrument firmware on the flash drive suppliedwith Lovis 2000 M/ME before mounting Lovis 2000 ME! Earlier versions donot support Lovis 2000 ME and other modules, and the DMA M/DSA M willdisplay Error 13. You have then to remove / disconnect the Lovis 2000 MEmodule again before running the update.2. Mount the Xsample into the master instrument. See Chapter 17.2.3. Mount the Lovis 2000 ME into the Module Housing Lovis ME. SeeChapter 6.4.4. Drip plate: Prepare and place the drip plate on top of the master instrumentand make sure it is correctly engaged, if applicable. When using the optionalstand for measuring assembly, the drip plate is not required to use. SeeChapter 15.3.5. Optional stand: Mount the optional stand for measuring assembly accordingto its supplied instructions, if applicable.6. Place the Module Housing with Lovis 2000 ME onto the drip plate / stand formeasuring assembly on top of the master instrument and check, whether allfour feet stand solidly on the master instrument’s cover. If not, adjust the feetaccording to Chapter 6.5.7. For assemblies with more modules than Lovis 2000 ME and samplechanger: arrange all components as described in the respective chapters inthis manual, if applicable.126 C72IB002EN-E

SECTION II : Flow-Through Systems17 Preparing Module(s) and Master Instrument8. Connect the cables:For standard assemblies consisting of Lovis 2000 M and a sample changeror DMA M/DSA M, Lovis 2000 ME and a sample changer, perform accordingto Chapter 6.6.For all measuring assemblies consisting of more components, refer to thechapter describing the respective setup.9. Xsample 452/352/122: Prepare the safety cover according to Chapter 17.2.10. Select the capillary and ball configuration according to your sample viscosity.Refer to Appendix A.2.11. Install the measuring system for flow through filling according to Chapter 9.5.Ensure, that the capillary is completely clean (inside and outside) beforeplacing it in the capillary block. Note down the serial number of the capillarybefore inserting it, you will need it later for creating the capillary in the"Capillary Administration".12. Establish the hose connections according to the respective chapter in thismanual. Regarding the waste hose and waste container, refer also to theinstruction manual of your Xsample model.13. Xsample 52/352/452: fill the solvent bottle(s).14. After completing the hardware setup, continue with Chapter 24.17.2 Preparing the Safety CoverEdge protectionThe edge protection is supplied with the Module Housing Lovis ME and must bemounted to prevent damage to the hose(s) which is (are) guided through the slit(1) in the metal part of the safety cover. The edge protection comes already in thecorrect length of 177 mm.Attach the edge protection (2) and fit it onto the edge of the hose guiding slitaccording to Fig. 17 - 3.C72IB002EN-E 127

17 Preparing Module(s) and Master Instrument SECTION II : Flow-Through Systems12Fig. 17 - 3Mounting the edge protectionCrush protectionTo meet the safety requirements, the crush protection Lovis/Xsample suppliedwith the Module Housing Lovis ME must be mounted on the Xsample safetycover for use in combination with Lovis 2000 ME. The following sample changersare affected:• Xsample 352/452• Xsample 122It is not required to mount the crush protection if:• Lovis 2000 ME is placed higher than the crush protection reaches, e.g. formeasuring assemblies, where Lovis 2000 ME is placed as topmost moduleon two instruments below it, like the assembly described in Chapter 20.1.• Lovis 2000 ME is not placed on the outside right in measuring assemblieswith more than one module, like the assemblies described in Chapter 20.2 toChapter 20.4.128 C72IB002EN-E

SECTION II : Flow-Through Systems17 Preparing Module(s) and Master InstrumentRefer to Fig. 17 - 4:2213 4Fig. 17 - 4Mounting the crush protection1. Unscrew the two M4x6 screws (1; Torx T10) from the top of the safety cover(2).2. Attach the crush protection Lovis/Xsample (3) and fix it with two screwsM4x8 (4; Torx T10).C72IB002EN-E 129

17 Preparing Module(s) and Master Instrument SECTION II : Flow-Through Systems17.3 Installing the Drip PlateNOTICEIn case an additional measuring module is placed on top of the masterinstrument with a built-in Xsample, install the drip plate. This additionalaccessory will avoid damages to the master instrument in the unlikely event ofa leakage.Supplied parts:21345Fig. 17 - 5Drip plate - supplied parts1 Drip plate2 Hose Viton 5x8 Iso Versinic3 Hose clamp Dmin 9.3 - Dmax 9.9 mm4 Drain nozzle5Fixing bracket with knurled screws - not applicable for measuring assembliesdescribed in this manualInstalling the drip plate1. Screw the supplied drain nozzle (1) into the thread at the back side of thedrip plate (2; open spanner 9 mm may be helpful). See Fig. 17 - 7.2. Place the drip plate on top of the master instrument and make sure it iscorrectly engaged. See Fig. 17 - 6.130 C72IB002EN-E

SECTION II : Flow-Through Systems17 Preparing Module(s) and Master InstrumentFig. 17 - 6Attaching the drip plate onto the master instrument3. Refer to Fig. 17 - 7: Plug the hose clamp (3) onto the waste hose (4).Connect the hose to the drain nozzle (1) on the back side of the drip plate (2)and secure it with the hose clamp. See Fig. 17 - 6. Trim the hose lengthdepending on the waste vessel and its location during installation of themeasuring assembly.3 142Fig. 17 - 7Connecting the waste hose to the drip plate4. Connect the hose to your waste container and secure it against slipping off.You can connect this hose e.g.• to the optional waste container 8 L HDPE together with the waste hose of theassembly• to the optional waste bottle SVM 0.5 L separately• to any other suitable vessel which allows to lead the hose always downwardsC72IB002EN-E 131

17 Preparing Module(s) and Master Instrument SECTION II : Flow-Through Systems5. Using an adapter Luer/UNF (3; supplied with Lovis accessory kit) or asuitable nozzle with 1/4"-28 male thread, the hose (1) can be properlyconnected to the optional available waste containers. Use a hose clamp (2)to secure the hose against slipping off.1 23Fig. 17 - 8Connecting the waste hose to the waste containerTIPWhen connecting the waste hose to the optional waste container 8 L HDPE,ensure that the adapter Luer/UNF is fixed in the insert of the screw cap. Thenattach the hose with hose clamp.17.4 Optional Waste Vessels17.4.1 Waste Container 8 L HDPETo collect non-hazardous waste liquids, an 8 L HDPE waste container is available.The container comes complete with screw cap and insert to connect several hosetypes. Further it is equipped with a stop cock on the outlet for easy draining orpouring the liquid. Additionally, two adapters UNF/screwed tube and an adapterLuer/UNF is supplied.The waste container can be placed either besides or behind the measuringassembly or - when using the optional stand for measuring assembly - under thestand besides the master instrument.How to connect the hoses• To connect a waste hose 2x4 mm or 2.5x4 mm, plug it onto one of theadapters UNF/screwed tube.• To connect a waste hose with 1/4"-28 UNF thread, remove one of theadapters from the insert and screw in the hose connector.132 C72IB002EN-E

SECTION II : Flow-Through Systems17 Preparing Module(s) and Master Instrument• To connect a waste hose 3x5 mm, attach the supplied adapter Luer coneonto the hose. Then plug the hose into an adapter Luer/UNF on the insert ofthe screw cap.• To connect the draining hose 5x8 from the drip plate, attach a hose clamponto the hose and plug the hose onto the adapter Luer/UNF. Secure thehose with the clamp against slipping off.13627485Fig. 17 - 9Waste container - supplied parts and connectors1 Waste container2 Stop cock (allows easily draining the waste container)3 Nozzle to connect a hose to the stop cock4 Screw capInsert with three 1/4"-28 UNF female threads (same part as for the waste bottle51 L glass)6 Adapter UNF/screwed tube (1 pc.)7 Adapter Luer cone (2 pcs.)8 Adapter Luer/1/4"-28 UNF male (2 pcs.)C72IB002EN-E 133

17 Preparing Module(s) and Master Instrument SECTION II : Flow-Through Systems17.4.2 Waste Bottle 1 L GlassTo collect also chemically aggressive waste liquids, a 1 L glass bottle is available.The bottle comes complete with screw cap and insert to connect several hosetypes.The waste bottle is intended for use with manually filled flow-through systems orwith systems including an Xsample 22. It can be placed either besides or behindthe measuring assembly.NOTICEDo not use this bottle with other Xsample systems. The filling volume is notenough to hold the contents (sample and solvent) of an entire magazine. Youcould use a 2 L glass bottle with GL45 thread instead (not available fromAnton Paar GmbH) or the Safety Waste Container (Mat. No. 87585).How to connect the hosesThe waste bottle glass 1 L serves the same insert with three 1/4"-28 UNF femalethreads as the waste container 8 L HDPE. See pos. 5 in Fig. 17 - 9.You can connect hoses with 1/4"-28 UNF male connectors directly to this insert,other hoses can be connected using the adapters supplied with Lovis or severalhose sets available for the measuring assemblies. Refer to Fig. 17 - 9.17.4.3 Replacing the Sealing GasketThis procedure applies for both, the waste container 8 L HDPE and the wastebottle 1 L glass.To ensure leak tightness of the closure, the bottom side of the insert is equippedwith a PTFE gasket. If required, this gasket can be replaced easily (sealing gasketPTFE GL45, Mat. No. 97027).134 C72IB002EN-E

SECTION II : Flow-Through Systems17 Preparing Module(s) and Master InstrumentJust remove the old gasket, clean the insert (1) and attach the new gasket (2).Ensure that the gasket is well engaged in the groove (see arrow). See Fig. 17 -10.1 2Fig. 17 - 10 Replacing the sealing gasket for the waste container/waste bottle17.5 CAN Power SupplyWhen setting up an assembly with external modules that require more power thanthe master instrument can deliver (see technical data), a CAN power supply (Mat.No. 100655) is required to ensure proper power supply and function of allcomponents.Modules and instruments on the CAN chain installed before the CAN powersupply are powered by the master, the rest is powered by the CAN power supply.This item comes completely with a 24 VDC power supply and a CAN cable 0.6 m.1234Fig. 17 - 11 CAN power supply1 CAN out (female plug)2 CAN in (male socket)power LED - lights green when the 24 VDC power supply is connected and3plugged to the mains supply4 24 VDC in - to connect the power supplyC72IB002EN-E 135

17 Preparing Module(s) and Master Instrument SECTION II : Flow-Through Systems17.6 General Information for InstallationHow to read the parts tables in the setup description:No. Item Supplied with Purpose Mat. No.1 Hose 530x3x2 FEP 2x1/4"-28 UNF LovisXsample needle to Lovis capillaryblock front25523• No.: number of the part in the figure above the table.• Item: describes the part• Supplied with: gives information, in which accessories the item can be found.Lovis means, that the part either can be found in the Lovis accessory kit or inone of the hose sets for the measuring assemblies as indicated.• Purpose: describes, where the part is mounted.• Mat. No.: this is the Anton Paar material number.Ordering hoses and adaptersFor each type of measuring assembly, a preconfigured hose set is available. Ifyou need only single hoses or adapters for replacement, they can also be orderedseparately. See the respective chapter in this manual and consult the LovisProduct Description List on the USB flash drive supplied with your Lovis.136 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample18 Lovis 2000 M + XsampleFor operating Lovis 2000 M together with a sample changer, the sample changeris positioned in the Lovis 2000 M Base Unit. The Lovis 2000 ME is mounted inthe additional Module Housing Lovis ME, which must be placed onto the baseunit.The Lovis 2000 M Base Unit works as "master" in such an assembly.NOTICEFor easy hose connection, the Lovis capillary block can be turned manually.To do this, ensure that the master instrument is switched off. Do not turn theLovis capillary block when the power is on, as this may cause damage to itsdrive.18.1 Lovis 2000 M + Xsample 352/452By default, this setup uses hoses with an outer diameter of 3 mm and an innerdiameter of 2 mm. Optionally, some hoses are available in a low volume designwith the same outer diameter, but an inner diameter of 1 mm. Fig. 18 - 1 showsthe standard hoses, but indicates where to connect the low volume hoses. For acomplete low volume setup see Fig. 19 - 4.Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessory kit)• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)18.1.1 Preparing Master Instrument and Modules1. Mount the Xsample 352/452 into the Lovis 2000 M Base Unit according toChapter 6.4.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.5. Connect the cables according to Chapter 6.6.6. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.C72IB002EN-E 137

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems7. Prepare the Xsample safety cover (edge protection, crush protection)according to Chapter 17.2.18.1.2 Connecting the HosesWARNINGLiquids leaking from the instrument due to improper installation may causeinjuries and risk of fire.• The sample changer may only be connected by authorized personnel whois fully trained to use the sample changer correctly and safely.138 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample2 / 2a1 / 1a849675waste vessel drain hose:(for example)5 3 210approx. 30 mm114Fig. 18 - 1Lovis + Xsample 452 hose connectionsNo. Item Supplied with Purpose Mat. No.1or1aHose 530x3x2 FEP 2x1/4"-28 UNF"C"or for low volume setup:Hose 530x3x1 FEP 2x1/4"-28 UNF(low volume)Lovis aoptional bXsample needle to Lovis capillaryblock front2552375860C72IB002EN-E 139

18 Lovis 2000 M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.2 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis25524or for low volume setup:Lovis capillary block rear toor2aHose 600x3x1 FEP 2x1/4"-28 UNF(low volume)optional Xsample sensor hose218743 Adapter UNF-UNF LovisConnects Xsample sensor hose andhose No. 2647904 Cable clip self-adhesive Lovis Hose guidance 62072a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 352/452(Mat. No. 101900).b. Optional: Item is part of the Low Volume Hose Set Lovis Xsample 352/452 (Mat. No. 101906)On Xsample 352/452 most hoses are pre-assembled. See the Xsample 352/452instruction manual.Needle - Lovis inlet1. Connect one end of the hose 530x3x2 (1) to the top of the Xsample 452needle. Bend the hose at this connected end into the shape of a slope. Itmay be helpful to attach the safety cover for this purpose. Then screw theother end to the Filling Adapter UNF Front Side at the Lovis capillary block.See Fig. 18 - 2.1Fig. 18 - 2Lovis + Xsample 352/452: mounting the hose between needle andLovis inletLovis outlet - Xsample sensor hose2. Lead the free end of the pre-assembled hose "BA" (sensor hose) of theXsample 352/452 (5) to the rear side and attach an adapter UNF-UNF (3).3. Connect one end of the hose 600x3x2 (2) to the Filling Adapter UNF BackSide Complete at the Lovis capillary block. Screw the other end into theadapter UNF-UNF (3).4. Fix the cable clip (4) on the master instrument’s casing approx. 30 mm belowthe upper edge.140 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample5. Fit the hose 600x3x2 (2) into this cable clip (4).Drip plate - waste container6. Trim the hose (10) coming from the drip plate to the required length, connectit to a waste vessel (15; e.g. as indicated in Fig. 18 - 1) and secure it againstslipping off.Air hose7. Connect the air hose of the Xsample 352/452 (6) to the AIR OUT connectorof the Lovis 2000 M Base Unit.8. Connect the waste hose (7) either to the optional safety waste container or tothe customer’s waste container according to the Xsample 352/452instruction manual.Rinsing hoses9. Prepare and connect the rinsing hose(s) (8) and the solvent bottle(s) (9)according to the Xsample 352/452 instruction manual.Completing the installation10. Attach the magazine according to the Xsample 452 instruction manual.11. Ensure that the edge protection and the crush protection are mounted on theXsample safety cover; see Chapter 17.2. Attach and use the safety coveraccording to the Xsample 352/452 instruction manual. Avoid to squeeze orkink the hose leading to the Lovis capillary block.12. To put the measuring assembly into operation, continue with Chapter 24.18.1.3 Low Volume SetupRequired equipment:• Low Volume Hose Set Lovis Xsample 352/452• Magazine for 6 mL vials 48 positions• Vials 6 mL polystyreneFor further details see the product description list (PDL) on the USB flash drivesupplied with your Lovis 2000 M/ME.Connect the low volume hoses as indicated in Fig. 18 - 1.Xsample 352: The 6 mL PS vials are not suitable for Xsample 352. For thisinstrument only the combination of low volume hoses and the standard 12 mLvials can be used.C72IB002EN-E 141

18 Lovis 2000 M + Xsample SECTION II : Flow-Through SystemsXsample 452: You can use only the low volume hoses or these hoses incombination with the low volume magazine and the 6 mL PS vials. Use the lowvolume hoses and the standard 12 mL glass vials, if your samples chemicallyattack polystyrene (PS).NOTICEDo not use the low volume magazine and vials if your samples chemicallyattack polystyrene (PS). In this case use the 12 mL glass vials together withthe standard 48 pos. magazine.To mount the low volume hoses, refer to Chapter 18.1.2. Chapter 19.1.2. Forassemblies with DMA M/DSA M see Chapter 19.1.2.A complete low volume setup with DMA M is shown in Fig. 19 - 4.Minimum required sample volume see Appendix A.4.18.2 Lovis 2000 M + Xsample 52Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessory kit)• Cutter or a pair of scissors• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)18.2.1 Preparing Master Instrument and Modules1. Mount the Xsample 52 into the Lovis 2000 M Base Unit according toChapter 6.4.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.5. Connect the cables according to Chapter 6.6.6. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.142 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample18.2.2 Connecting the HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample filling and rinsing unit may only be connected by authorizedpersonnel who is fully trained to use this unit correctly and safely.423approx.10 mm68A19cable clip on capillaryblock bottom front side:4 5approx.15 mmwaste vessel drain hose:(for example)approx. 30 mm4647Fig. 18 - 3Lovis + Xsample 52 hose connectionsC72IB002EN-E 143

18 Lovis 2000 M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis a Lovis filling adapter UNF to Loviscapillary block front (sample filling 25523hose)2 Hose 300x3x2 FEP 1x1/4"-28 UNF Lovissample vial to Lovis filling adapterUNF (sample filling hose)1090473 Hose 600x3x2 FEP 2x1/4"-28 UNF LovisLovis capillary block rear to "Cell"connector on valve block Xsample 25524524 Cable clip self-adhesive Lovis hose guidance, 3 pcs. 6207256Hose Silicone 2x4l = approx. 200 mmHose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerXsample 52Drip plate7 Waste Bottle SVM 3000 0.5 L Lovisair hose, Lovis AIR connector to AIRIN on the Xsample valve blockdrain hose from drip platewaste vessel for drain hose; can also be used to elevate theposition of the sample vessel51273725462130717822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 52 (Mat. No. 101901).1. Mount the cable clips as indicated in Fig. 18 - 3: Fix one cable clip (4) on themaster instrument’s casing, the second cable clip (4) on the module housingME and the third cable clip (4) on the front bottom side of the capillary block.Sample vessel - Lovis inlet (sample filling hose)2. The filling adapter (A) mounted on the Lovis has a continuous UNF thread.Connect the hose 300x3x2 FEP (1) to the bottom of this filling adapter andtighten it.3. Screw one end of the hose 600x3x2 (2) to the Filling Adapter UNF Front Sideof the Lovis capillary block.4. Carefully bend the hose as shown in Fig. 18 - 4 and place it into the cableclips (4) on the bottom of the capillary block and on the module housing ME.Connect the free end of the hose (2) to the top of the Lovis filling adapter. (A)Tighten the connector.144 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample244A1Fig. 18 - 4Lovis + Xsample 52: Connecting the sample filling hoseTIPFor low viscous samples (below approx. 5 mPa.s), elevate the position of thesample vessel. If not connected to the drip plate, use e.g. the waste bottlesupplied with the Lovis to place the sample vessel on. Or take something similarwith a height of approx. 10 cm, e.g. the box of a standard bottle. This improvesthe stability of the liquid column in the system and allows therefore a betterrepeatability of results. See Fig. 18 - 5.C72IB002EN-E 145

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systemsapprox. 10 cmFig. 18 - 5Lovis + Xsample 52: sample vessel on elevated positionLovis outlet - Xsample valve block5. Screw one connector of the hose 600x3x2 FEP (3) into the Filling AdapterUNF Back Side Complete of the Lovis capillary block, the other one in to the"Cell" connector on the Xsample 52 valve block.6. Fit the hose 600x3x2 (3) into the cable clip (4) on the master instrument’shousing.Drip plate - waste container7. Trim the hose (6) coming from the drip plate to the required length, connect itto a waste vessel (7; e.g. the waste vessel supplied with Lovis 2000 M/ME ora different vessel as indicated in Fig. 18 - 1) and secure it against slippingoff.Air hose8. Cut a piece of approx. 200 mm from the Silicone hose 2x4 (5).9. Connect this hose to the AIR nozzle of the Lovis 2000 M Base Unit and tothe "AIR IN" connector on the Xsample 52 valve block.Rinsing hose(s)10. Prepare and connect the rinsing hose(s) (8) and the solvent bottle(s) (9)according to the Xsample 52 instruction manual.Completing the installation11. To put the measuring assembly into operation, continue with Chapter 24.146 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample18.3 Lovis 2000 M + Xsample 122There are two filling options for sample changers with peristaltic pump:Filling by pressure: The sample goes first from the needle to the pump and thenfrom the pump to the instrument. Use this option for highly viscous or volatilesamples.Filling by suction: The sample goes first to the instrument and then through thepump.Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessory kit)• Cutter or a pair of scissors• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)18.3.1 Preparing Master Instrument and Modules1. Mount the Xsample 122 into the Lovis 2000 M Base Unit according to theXsample 122 instruction manual.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.5. For assemblies with pressurized filling: Mount the cable clips on the rearside of the Module Housing Lovis ME according to Fig. 18 - 6. All other clipsmount as indicated in the respective sections.approx. 45 mmapprox. 35 mmapprox. 110 mmFig. 18 - 6Lovis Module Housing ME, mounting the cable clipsC72IB002EN-E 147

18 Lovis 2000 M + Xsample SECTION II : Flow-Through SystemsFor assemblies with filling by suction: Mount the cable clips as indicatedin the respective description.6. Connect the cables according to Chapter 6.6.7. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.8. Prepare the Xsample safety cover (edge protection, crush protection)according to Chapter 17.2.18.3.2 Connecting the Standard HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample changer may only be connected by authorized personnel whois fully trained to use the sample changer correctly and safely.Note: Fig. 18 - 7 and Fig. 18 - 9 show two possibilities how to connect the drainhose from the drip plate to a waste container. See also the information in thedescription of this step.148 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample18.3.2.1 Filling by Pressure65129141011135 4 333 2 16 9 7 9 8optional waste container:8131213Fig. 18 - 7Lovis + Xsample 122, standard hoses, pressurized fillingC72IB002EN-E 149

18 Lovis 2000 M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1Hose Tygon 2375, 1.6x3.2 l = approx. 270 mm2 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1223Hose Silicone 2x4 alternativelyHose 2x4 Viton® l = approx. 400 mmNeedle - pump - Lovis inletXsample 122 needle to pump hose 67540Xsample 122Lovisconnector between Tygon hose andpump hosepump hose1. Cut a piece of approx. 270 mm from the Tygon hose 1.6x3.2.2. Connect the steel tube 2.1x2.4x20 mm (2) to the Tygon hose (1).6798251273546284 Adapter UNF/Hose (Female/Male) Lovis a connector for filling hose 267395 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis filling hose 255246 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis7 Adapter UNF/UNF LovisLovis capillary block rear side toadapter UNF/UNFconnects the hose 350 mm comingfrom Lovis capillary block rear sidewith the hose 600 mm to waste25525647908 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovisadapter UNF/UNF to wastecontainer255249 Cable clip self-adhesive Lovis hose guidance 6207210Hose Silicone 3x5 l = approx. 200 mmLovis air hose 5081411 Adapter Luer cone Lovis tip for air hose 6386312Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130713 Waste container 8 L HDPE Optional waste container 10597914 Waste Bottle SVM 3000 0.5 L Loviswaste vessel for drain hose;can be used alternatively if you donot want to connect the drain hoseto the large waste container17822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 22/122(Mat. No. 101902).3. Cut a piece of approx. 400 mm from the Silicone hose 2x4 (3; from theXsample 122 accessories). Alternatively, if you need a better chemicalresistance, you can also use the Viton® hose 2x4 (from the Lovisaccessories).4. Plug the Silicone (or alternatively Viton®) hose (3) onto the free end of thesteel tube (2).150 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample5. Attach an adapter UNF/Hose (4) onto the free end of the Silicone (or Viton®)hose.6. Plug the Tygon end of this assembled hose onto the top of the needle.TIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.7. Turn the pump lever into horizontal position and remove the cartridge. Coilthe Silicone (or Viton®) part of this assembled hose around the peristalticpump. Attach the cartridge, hold it in place and pull the two hose endsslightly downwards, so that they are positioned in the groove of the cartridge.Then turn the pump lever to vertical position.8. To hold the Silicone (or Viton®) hose in position, place it in the tube holderbelow the Xsample needle.9. Refer to Fig. 18 - 8: Connect the hose 530x3x2 FEP 2x1/4"-28 UNF (5) tothe adapter UNF/hose of the pump hose. Lead the hose 530X3X2 upwards.Carefully bend this hose into the shape of a loop and connect the free end tothe Filling Adapter UNF Front Side on the Lovis capillary block. It may behelpful to attach the safety cover for this purpose.5Fig. 18 - 8Connecting the hose between pump hose and LovisLovis outlet - waste container10. Connect the hose 350x3x2 FEP 2x1/4"-28 UNF (6) and the hose 600x3x2FEP 2x1/4"-28 UNF (8) with an adapter UNF/UNF (7).11. Screw the free end of the hose 350x3x2 into the Filling Adapter UNF BackSide Complete of the Lovis capillary block. Bend the hose as indicated inFig. 18 - 7 and secure it by placing the hose connectors with their springs inthe cable clips.C72IB002EN-E 151

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems12. Lead the free end of the hose 600x3x2 to a waste container and secure thehose against slipping off. When using the optional waste container 8 LHDPE, you can screw the connector of the hose directly into the screw capof this container.Drip plate - waste container13. Trim the hose (12) coming from the drip plate to the required length andconnect it either to the waste container 8 L HDPE (13). If you want toconnect it to a separate vessel, you can use e.g. the optional available wastebottle SVM 0.5 L (14) or any other suitable vessel. Secure the hose againstslipping off.Air hose14. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (7). Attach anadapter Luer cone (8) onto one end.15. Connect this hose to the AIR outlet of the Lovis 2000 M Base Unit.Completing the installation16. Attach the magazine and the safety cover according to the Xsample 122instruction manual. Ensure that the edge protection and the crush protectionare mounted on the Xsample safety cover; see Chapter 17.2. Lead the hose,which is connected between needle, pump and the front side of the Loviscapillary block, through the slit in the metal part of the safety cover. Avoid tosqueeze or kink the hose.17. To put the measuring assembly into operation, continue with Chapter 24.152 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample18.3.2.2 Filling by Suction13210412768approx. 30 mm6 5 34Optionalwaste container:691161011Fig. 18 - 9Lovis + Xsample 122 standard hoses, filling by suctionC72IB002EN-E 153

18 Lovis 2000 M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Adapter UNF/screwed tube Lovis a for connecting the Tygon filling hose 188072Hose Tygon 2375, 1.6x3.2 mm;l = approx. 450 mmXsample 122Lovisfilling hose, needle - Lovis capillaryblock675403 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis Lovis capillary block - pump hose 255244 Cable clip self-adhesive Lovis hose guidance 620725 Adapter UNF/Hose (Female/Male) Lovis connector for filling hose 267396Hose Silicone 2x4 mmalternativelyHose 2x4 Viton® l = min. 600 mm, depending onlocation of the waste containerXsample 122Lovispump and waste hose51273546287Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 508148 Adapter Luer cone Lovis tip for air hose 638639 Adapter UNF/screwed tube10Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerLovis;opt. wastecontainerDrip plateto connect the pump hose to theoptional waste containerdrain hose from drip plate18807725462130711 Waste container 8 L HDPE Optional waste container 10597912 Waste Bottle SVM 3000 0.5 L Lovis13Tube d=2.1 D=2.4 L=20; 1.4571Loviswaste vessel for drain hose;can be used alternatively if you donot want to connect the drain hoseto the large waste containeroptional use to split the Siliconehose 2x4 used as pump and wastehose.1782267982a.Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 22/122(Mat. No. 101902).Needle - Lovis inlet1. Screw an adapter UNF/screwed tube (1) into the Filling Adapter UNF FrontSide of the Lovis capillary block.2. Cut a piece of approx. 450 mm from the Tygon hose 1.6x3.2.154 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample3. Refer to Fig. 18 - 10: Plug the Tygon hose (2) onto the needle top ofXsample 122. Bend the hose into the shape of a loop and plug the free endonto the adapter UNF/screwed tube (1) on the front side of the Loviscapillary block. It may be helpful to attach the safety cover for this purpose.12Fig. 18 - 10 Mounting the hose between Xsample needle and LovisTIPTo remove the Tygon hose, do not pull it. This may damage the hose and the hosecannot be re-used. Move the hose from the connector using your finger tips.Lovis outlet - pump - waste container4. Screw an adapter UNF/hose (5) onto one end of the hose 600x3x2 FEP 2x1/4"-28 UNF (3).5. Connect this hose to the Filling Adapter UNF Back Side Complete of theLovis capillary block.6. Cut a piece of min. 600 mm (or the required length to reach your wastecontainer) from the Silicone hose 2x4.TIPYou can split the Silicone hose 2x4 (6) into two parts of approx. 250 mm and thelength you require to reach the waste container. Connect the two pieces with atube d=2.1 D=2.4 L=20 (13). Advantages: easy replacement of the hose part usedin the peristaltic pump, less consumption of hose material. See Fig. 18 - 11.pump hose6to waste container613Fig. 18 - 11 Optional splitting the waste hoseC72IB002EN-E 155

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems7. Plug one end of this hose (or if you have splitted to hose the free end of thehose with l = 250 mm) onto the adapter UNF/hose (5).8. Turn the pump lever to the horizontal position and remove the cartridge. Coilthe Silicone hose around the peristaltic pump as shown in Fig. 19 - 11.Attach the cartridge, hold it in place and pull the two hose ends slightlydownwards, so that they are positioned in the groove of the cartridge. Thenturn the pump lever to vertical position.9. Lead the free end of the Silicone hose to the waste container. Secure thehose against slipping off.If you use the optional waste container 8 L HDPE (11): Screw an adapterUNF/screwed tube (9) into the insert of the bottle’s screw cap. Plug the hose(6) to this adapter.10. Fix the cable clip (4) on the master instrument’s housing as indicated inFig. 18 - 9.11. Fit the hose 600x3x2 (3) into the cable clip (4).Drip plate - waste container12. Trim the hose (10) coming from the drip plate to the required length andconnect it either to the waste container 8 L HDPE (11). If you want to connectit to a separate vessel, you can use e.g. the optional available waste bottleSVM 0.5 L (12) or any other suitable vessel. Secure the hose againstslipping off.Air hose13. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (7). Attach anadapter Luer cone (8) onto one end.14. Connect this hose to the AIR outlet of the Lovis 2000 M Base Unit.Completing the installation15. Attach the magazine according to the Xsample 122 instruction manual.Ensure that the edge protection and the crush protection are mounted on theXsample safety cover; see Chapter 17.2. Lead the hose, which is connectedbetween the needle and the front side of the Lovis capillary block, throughthe slit in the metal part of the safety cover. Avoid to squeeze or kink thehose. Attach the safety cover (refer to the instruction manual of Xsample122).16. To put the measuring assembly into operation, continue with Chapter 24.156 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample18.3.3 Connecting Chemically Resistant HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample changer may only be connected by authorized personnel whois fully trained to use the sample changer correctly and safely.Required equipment:• Retrofit kit for Xsample 122 Chemical (Mat. No. 96878) or• Retrofit kit for Xsample 122 Petro (Mat. No. 96880)• Xsample 122 instruction manual• Angular screwdriver Torx T10 (supplied with the chemical retrofit kits andwith module housing ME)TIPIf your application requires an improved chemical resistance, a Lovis chemicalresistance upgrade set (Mat. No. 101899) is available. See Appendix B.3.Refer to the Xsample 122 instruction manual:Follow and adhere to all safety instructions and additional informations given insection "Installing the Optional Kit for Resistant Hoses.1. Switch off the master instrument.2. Replace the standard steel needle by the chemically resistant Hastelloyneedle according to the Xsample 122 instruction manual, section "Installingthe Optional Kit for Resistant Hoses - Exchanging the Needle".3. In case of retrofitting, remove all standard hoses except the hose connectedto the rear side of the Lovis capillary block.18.3.3.1 Filling by PressureConnect the hoses according to Fig. 18 - 12 and the table of parts below. Referalso to Chapter 18.3.2, further to the Xsample 122 instruction manualC72IB002EN-E 157

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems78261164pump hose:12 13755434cartridge:X68 11 9 11 10optional waste container:10131415Fig. 18 - 12 Lovis + Xsample 122, chemical resistant hoses, pressurized filling158 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + XsampleNo. Item Supplied with Purpose Mat. No.1 Hastelloy needle Retrofit kitchemical resistant needle with UNFthread924212 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis a needle to pump hose 255253Hose Norprene Chemical 3.2x6.4 l = approx. 250 mm)orHose Norprene A-60-G 3.2x6.4 l = approx. 250 mm)Retrofit kitpump hose, chemical resistant96878or968804 Adapter UNF/hose Retrofit kit hose connectors, pump hose 267395 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kit6 Cartridge PVDF 3.2x6.4 Retrofit kit7 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis8 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis9 Adapter UNF/UNF Lovisto secure the pump hose on theadapter UNF/hosepump cartridge for chemicalresistant hosespump hose - Lovis capillary blockfront sideLovis capillary block rear side toadapter UNF/UNFconnects the hose 350 mm comingfrom Lovis capillary block rear sidewith the hose 600 mm to waste215319408625524255256479010 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovisadapter UNF/UNF to wastecontainer2552411 Cable clip self-adhesive Lovis hose guidance 6207212Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081413 Adapter Luer cone Lovis tip for air hose 6386314Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130715 Waste container 8 L HDPE Optional waste container 10597916 Waste Bottle SVM 3000 0.5 L Loviswaste vessel for drain hose;can be used alternatively if you donot want to connect the drain hoseto the large waste container17822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 22/122(Mat. No. 101902).C72IB002EN-E 159

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems18.3.3.2 Filling by SuctionConnect the hoses according to Fig. 18 - 13 and the table of parts below. Referalso to Chapter 18.3.2, further to the Xsample 122 instruction manual.160 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample23814111614812pump hose:13566approx. 30 mm55cartridge:1147X14optional waste container:1513810159Fig. 18 - 13 Lovis + Xsample 122, chemical resistant hoses, filling by suctionC72IB002EN-E 161

18 Lovis 2000 M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.chemical resistant needle with UNF1 Hastelloy needle Retrofit kitthread2 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis a needle to Lovis capillary block frontside3Hose 600x3x2 FEP 2x1/4"-28 UNFLoviscapillary rear front side to pumphose9242125523255244Hose Norprene Chemical 3.2x6.4 l = approx. 250 mmorHose Norprene A-60-G 3.2x6.4 l = approx. 250 mmRetrofit kitpump hose, chemical resistant96878or968805 Adapter UNF/hose Retrofit kit hose connectors, pump hose 267396 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kit7 Cartridge PVDF 3.2x6.4 Retrofit kit8 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Adapter UNF/UNF Lovisto secure the pump hose on theadapter UNF/hosepump cartridge for chemicalresistant hoseswaste hose; pump hose adapterUNF/hose to adapter UNF/UNFconnects the hose 530 mm comingfrom the pump with the hose600 mm to waste2153194086255236479010 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; adapter UNF/UNF towaste container2552411 Cable clip self-adhesive Lovis hose guidance 6207212Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081413 Adapter Luer cone Lovis tip for air hose 6386314Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130715 Waste container 8 L HDPE Optional waste container 10597916 Waste Bottle SVM 3000 0.5 L Loviswaste vessel for drain hose;can be used alternatively if you donot want to connect the drain hoseto the large waste container17822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 22/122(Mat. No. 101902).162 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + Xsample18.4 Lovis 2000 M + Xsample 22There are two filling options for sample changers with peristaltic pump:Filling by pressure: The sample goes first from the needle to the pump and thenfrom the pump to the instrument.Filling by suction: The sample goes first to the instrument and then through thepump.Required equipment:• Angular screwdriver Torx T20 (from Lovis accessories)• Cutter or a pair of scissors18.4.1 Preparing Master Instrument and Modules1. Mount the Xsample 22 into the Lovis 2000 M Base Unit according to theXsample instruction manual.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.5. Connect the cables according to Chapter 6.6.6. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.18.4.2 Connecting the Standard HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample filling unit may only be connected by authorized personnelwho is fully trained to use the sample filling unit correctly and safely.C72IB002EN-E 163

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems18.4.2.1 Filling by Pressure6 78118122191054matches withfilling adapter6542 3 48waste vessel drain hose:(for example)11approx. 10 mm8approx. 30 mm138Fig. 18 - 14 Lovis + Xsample 22, standard hoses, pressurized filling164 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + XsampleNo. Item Supplied with Purpose Mat. No.1 Needle Xsample 222Hose Tygon 2375, 1.6x3.2 mml = approx. 250 mmXsample 223 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 224Hose Silicone 2x4 mm; alternativelyHose 2x4 Viton® l = approx. 250 mmXsample 22Lovis1. Fix the cable clips (8) on the module housing ME and on the Lovis 2000 Mbase unit’s housing as indicated in Fig. 18 - 14.Needle - pump - Lovis inlet2. Cut a piece of approx. 270 mm from the Tygon hose 1.6x3.2 (2).3. Attach one end of this hose to the needle (1).sample filling needle, to be placed inthe sample beakerhose between needle and pumphoseconnector between Tygon hose andpump hosepump hose67394675406798251273546285 Adapter UNF/Hose (Female/Male) Lovis a connector for filling hose 267396 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis filling hose 255247 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis waste hose 255248 Cable clip self-adhesive Lovis hose guidance, 3 # 620729Hose Silicone 3x5 mm; i = approx.200 mmLovis air hose 5081410 Adapter Luer cone Lovis tip for air hose 6386311Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130712 Waste bottle 1 L glass Optional waste container 10165513 Waste Bottle SVM 3000 0.5 L Lovis waste vessel for drain hose; 17822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 22/122(Mat. No. 101902).4. Connect the tube 2.1x2.4x20 mm (3) to the other end of the Tygon hose (2).TIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.5. Cut a piece of approx. 250 mm from the Silicone hose 2x4 (4).6. Connect the Silicone hose (4) to the free end of the tube 2.1x2.4x20 mm (3).7. Attach an adapter UNF/hose (5) onto the free end of the Silicone hose (4).C72IB002EN-E 165

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems8. Turn the pump lever to the horizontal position and remove the cartridge. Coilthe Silicone hose around the peristaltic pump as shown in Fig. 18 - 14.Attach the cartridge, hold it in place and pull the two hose ends slightlydownwards, so that they are positioned in the groove of the cartridge. Thenturn the pump lever to vertical position.9. Connect a hose 600x3x2 FEP (6) to the adapter UNF/hose.10. Refer to Fig. 18 - 15: Lead the end of the hose 600x3x2 (6) you haveconnected to the adapter UNF/hose upwards and bend it carefully aroundthe edge of the Lovis 2000 M Base Unit. Place the hose into cable clipmounted on the Lovis 2000 M Base Unit (8). Then bend the hose in front ofthe Lovis capillary block into the shape of a loop. Screw the hose connectorinto the Filling Adapter UNF Front Side at the Lovis capillary block. To holdthe hose in place, fit it into the cable clip (8) mounted on the module housingME.868Fig. 18 - 15 Lovis + Xsample 22 - connecting the FEP hose to Lovis11. Fit the hose 600x3x2 (6) into the cable clips (8).Lovis outlet - waste container12. Connect a further hose 600x3x2 FEP (7) to the Filling Adapter UNF BackSide Complete of the Lovis capillary block.13. Fit the hose 600x3x2 (7) into the cable clip (8) and lead it into the wastevessel. Secure the hose, to prevent it from slipping off. When using the optional waste bottle glass 1 L, you can connect this hosedirectly to the cap of this bottle.166 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + XsampleDrip plate - waste container14. Trim the hose (11) coming from the drip plate to the required length. Connectit e.g. to the optional available waste bottle SVM 0.5 L (13) or any othersuitable vessel. Secure the hose against slipping off.Air hose15. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (9). Attach anadapter Luer cone (10) onto one end.16. Connect this hose to the AIR outlet of the Lovis 2000 M Base Unit.Completing the installation17. To put the measuring assembly into operation, continue with Chapter 24.C72IB002EN-E 167

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems18.4.2.2 Filling by Suction16311771028945Optional waste bottle:4456matches withfilling adapter10712waste vessel drain hose:(for example)approx. 10 mm117approx. 30 mm137Fig. 18 - 16 Lovis + Xsample 22, standard hoses, filling by suction168 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + XsampleNo. Item Supplied with Purpose Mat. No.1 Adapter UNF/screwed tube Lovis a hose connector front side of Lovis18807capillary block2 Needle Xsample 223Hose Tygon 2375, 1.6x3.2 mml = approx. 500 mmXsample 22sample filling needle, to be placed inthe sample beakerhose between needle and pumphose6739467540Hose Silicone 2x4 mmXsample 22512734alternativelypump/waste hoseHose 2x4 Viton® Lovis54628l = approx. 600 mm)5 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267396 Hose 600x3x2 FEP 2x1/4"-28 UNF LovisLovis capillary block rear side topump hose255247 Cable clip self-adhesive Lovis hose guidance, 3 # 620728Hose Silicone 3x5 mmi = approx. 200 mmLovis air hose 508149 Adapter Luer cone Lovis tip for air hose 6386310 Adapter UNF/screwed tube Lovis11Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip plateto connect the waste hose to theoptional waste bottle glass 1 Ldrain hose from drip plate18807725462130712 Waste bottle 1 L glass Optional waste container 10165513 Waste Bottle SVM 3000 0.5 L Lovis waste vessel for drain hose; 17822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 22/122(Mat. No. 101902).1. Fix the cable clips (7) on the module housing ME and on the masterinstrument’s housing as indicated in Fig. 18 - 16.Needle - Lovis inlet2. Screw an adapter UNF/screwed tube (1) into the Filling Adapter UNF FrontSide of the Lovis capillary block.3. Cut a piece of approx. 500 mm from the Tygon hose 1.6x3.2 (3).4. Refer to Fig. 18 - 17: Plug this Tygon hose (3) onto the adapter UNF/screwed tube (1). Bend the hose in front of the Lovis capillary block into theshape of a loop. To hold the hose in place, fit it into the cable clip (7)mounted on the module housing ME. Connect the free end of the hose to theneedle (2). Place the hose into cable clip mounted on theLovis 2000 M Base Unit (8).C72IB002EN-E 169

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems1377Fig. 18 - 17 Lovis + Xsample 22 - connecting the Tygon hose between needleand LovisTIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.Lovis outlet - pump - waste container5. Connect a hose 600x3x2 FEP (6) to the Filling Adapter UNF Back SideComplete of the Lovis capillary block.6. Cut a piece of approx. 600 mm from the Silicone hose 2x4 (4).7. Attach an adapter UNF/hose (5) to one end of this hose.8. Connect this end of the Silicone hose (4) to the hose 600x3x2 FEP (6) whichcomes from the rear side of the Lovis capillary block.9. Turn the pump lever to the horizontal position and remove the cartridge. Coilthe Silicone hose around the peristaltic pump as shown in Fig. 18 - 16.Attach the cartridge, hold it in place and pull the two hose ends slightlydownwards, so that they are positioned in the groove of the cartridge. Thenturn the pump lever to vertical position.10. Fit the hose 600x3x2 (6) into the cable clip (7).11. Lead the free end of the Silicone hose (4) to the waste container. Fit the hoseinto the same cable clip (7) where you have placed the hose 600x3x2 (6).Secure the hose against slipping off.If you use the optional waste bottle glass 1 L (10): Screw an adapter UNF/screwed tube (10) into the insert of the bottle’s screw cap. Plug the Siliconehose (4) onto this adapter.170 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + XsampleDrip plate - waste container12. Trim the hose (11) coming from the drip plate to the required length. Connectit e.g. to the optional available waste bottle SVM 0.5 L (13) or any othersuitable vessel. Secure the hose against slipping off.Air hose13. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (8). Attach anadapter Luer cone (9) onto one end.14. Connect this hose to the AIR outlet of the Lovis 2000 M Base Unit.Completing the installation15. To put the measuring assembly into operation, continue with Chapter 24.18.4.3 Connecting Chemically Resistant HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample filling unit may only be connected by authorized personnelwho is fully trained to use the sample filling unit correctly and safely.Required equipment:• Retrofit kit for Xsample 22 Chemical (Mat. No. 96879) or• Retrofit kit for Xsample 22 Petro (Mat. No. 96881)• Xsample 22 instruction manualTIPIf your application requires an improved chemical resistance, a Lovis chemicalresistance upgrade set (Mat. No. 101899) is available. See Appendix B.3.Refer to the Xsample 22 instruction manual:Follow and adhere to all safety instructions and additional informations given insection "Installing the Optional Kit for Resistant Hoses".1. Switch off the master instrument.2. In case of retrofitting, remove all standard hoses except the FEP hoseconnected to the rear side of the Lovis capillary block.18.4.3.1 Filling by PressureConnect the hoses according to Fig. 18 - 18 and the table of parts below. Referalso to Chapter 18.4.2, further to the Xsample 22 instruction manual.C72IB002EN-E 171

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systems6 710885111924pump hose:43matches withfilling adapter8323approx. 10 mm8waste vessel drain hose:(for example)approx. 30 mm108cartridge:125XFig. 18 - 18 Lovis + Xsample 22, chemical resistant hoses, pressurized filling172 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + XsampleNo. Item Supplied with Purpose Mat. No.12Hose 190x3x2 PTFE 1x1/4"-28 UNForHose 650x3x2 FEP 1x1/4"-28 UNFHose Norprene Chemical 3.2x6.4 l = approx. 250 mmorHose Norprene A-60-G 3.2x6.4 l = approx. 250 mmXsample 22LovisRetrofit kitsample vessel to pump hosehose 650x3x2 can be trimmed to therequired length depending on sizeand position of the sample vesselpump hose, chemical resistant784532480996878or968803 Adapter UNF/hose Retrofit kit hose connectors, pump hose 267394 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kit5 Cartridge PVDF 3.2x6.4 Retrofit kit6 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovisto secure the pump hose on theadapter UNF/hosepump cartridge for chemicalresistant hosespump hose to Lovis capillary blockfront side2153194086255247 Hose 600x3x2 FEP 2x1/4"-28 UNF LovisLovis capillary block rear side towaste container255248 Cable clip self-adhesive Lovis hose guidances, 3 # 62072910Hose Silicone 3x5 mml = approx. 200 mmHose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerLovis air hose 50814Drip platedrain hose from drip plate725462130711 Waste bottle 1 L glass Optional waste container 10165512 Waste Bottle SVM 3000 0.5 L Lovis waste vessel for drain hose; 1782218.4.3.2 Filling by SuctionConnect the hoses according to Fig. 18 - 18 and the table of parts below. Referalso to Chapter 18.4.2, further to the Xsample 22 instruction manual.Note - Preparing and connecting the sample filling hose:Connect the hose 190x3x2 PTFE (or 650x3x2 FEP - trim this hose to the requiredlength depending on the size and position of your sample beaker) (1) to thebottom of the Lovis filling adapter (A). Connect the hose 530x3x2 FEP to the topof the filling adapter and tighten the connector. Bend the hose as shown inFig. 18 - 18. Connect the free end of this hose to the Filling Adapter UNF FrontSide at the Lovis capillary block. To hold the hose in place, fit it into the cable clip(9) mounted on the bottom of the capillary block.C72IB002EN-E 173

18 Lovis 2000 M + Xsample SECTION II : Flow-Through Systemscable clip on capillaryblock bottom front side:2 7approx.15 mm1289A911210pump hose:9365 455443approx. 10 mm9waste vessel drain hose:(for example)approx. 30 mm119cartridge:136XFig. 18 - 19 Lovis + Xsample 22, chemical resistant hoses, filling by suction174 C72IB002EN-E

SECTION II : Flow-Through Systems18 Lovis 2000 M + XsampleNo. Item Supplied with Purpose Mat. No.filling hose, sample vessel to hose1 Hose 190x3x2 PTFE 1x1/4"-28 UNF Xsample 22(2)2 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis a hose from hose (1) to Lovis capillaryblock front side3Hose Norprene Chemical 3.2x6.4 l = approx. 250 mmorHose Norprene A-60-G 3.2x6.4 l = approx. 250 mmRetrofit kitpump hose, chemical resistant784532552396878or968804 Adapter UNF/hose Retrofit kit hose connectors, pump hose 267395 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kit6 Cartridge PVDF 3.2x6.4 Retrofit kit7 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovisto secure the pump hose on theadapter UNF/hosepump cartridge for chemicalresistant hosesreplaces the standard cartridgeLovis capillary block rear side topump hose2153194086255248 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; pump hose to wastecontainer255249 Cable clip self-adhesive Lovis hose guidance, 3 # 620721011Hose Silicone 3x5 mm l = approx. 200 mmHose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerLovis air hose 50814Drip platedrain hose from drip plate725462130712 Waste bottle 1 L glass Optional waste container 10165513 Waste Bottle SVM 3000 0.5 L Lovis waste vessel for drain hose 17822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+Xsample 22/122(Mat. No. 101902).C72IB002EN-E 175

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M +XsampleFor operating Lovis 2000 ME together with a DMA M / DSA M and a samplechanger, the sample changer must always be mounted into the density meter.The Lovis 2000 ME is to be mounted into the additional ModuleHousing Lovis ME, which must be placed onto the density meter.In such an assembly the density meter works as "master".NOTICEFor more convenient connection of the hoses, the Lovis capillary block can beturned manually. To do this, ensure that the master instrument is switched off.Do not turn the Lovis capillary block when the power is on, as this may causedamage to its drive.19.1 Lovis 2000 ME + DMA M + Xsample 352/452Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)19.1.1 Preparing Master Instrument and Modules1. Mount the Xsample 352/452 into the DMA M according to the Xsample 352/452 instruction manual.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Prepare the Xsample safety cover (edge protection, crush protection)according to Chapter 17.2.5. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.6. Connect the cables according to Chapter 6.6.7. Prepare the Xsample safety cover according to Chapter 17.2.8. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.176 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample19.1.2 Connecting the HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample changer may only be connected by authorized personnel whois fully trained to use the sample changer correctly and safely.4 / 4a3 / 3a2 / 2a9611112810approx. 30 mm7546Fig. 19 - 1Lovis + DMA M + Xsample 352/452 hose connectionsC72IB002EN-E 177

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4"2or2a3or3a4or4aHose 300x3x2 PTFE 2x1/4"-28UNF, marked "AA"or for low volume setup:Hose 300x3x1 FEP 2x1/4"-28 UNF;marked "B"Hose 530x3x2 FEP 2x1/4"-28 UNF"C"or for low volume setup:Hose 530x3x1 FEP 2x1/4"-28 UNF(low volume)Hose 600x3x2 FEP 2x1/4"-28 UNFor for low volume setup:Hose 600x3x1 FEP 2x1/4"-28 UNF(low volume)Xsample 352/452Xsample 352/452optional aLovis boptionalLovisoptionalFilling adapters for DMA M 12219Hose between needle and densitycell inletdensity cell outlet to Lovis capillaryblock front sideLovis capillary block rear side toXsample sensor hose344321875255237586025524218745 Adapter UNF/UNF LovisConnects Xsample sensor hose andhose No. 4647906 Cable clip self-adhesive Lovis Hose guidance 62072a. Optional: Item is part of the Low Volume Hose Set Lovis+Xsample 352/452 (Mat. No. 101906)b. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 352/452(Mat. No. 101903).On Xsample 352/452 most hoses are pre-assembled. See the Xsample 352/452instruction manual.1. Replace the currently installed injection adapters of the DMA M by theinjection adapters UNF (1) supplied with the Xsample 352/452. To do thisrefer to the Xsample 352/452 instruction manual.Needle - master instrument inlet2. Connect one end of the hose 300x3x2 PTFE (2) to the top of the Xsampleneedle, the other end to the inlet of the density cell.Master instrument outlet - Lovis inlet3. Connect one end of the hose 530x3x2 (3) to the outlet of the density cell.Bend the hose at this connected end upwards and in front of the Loviscapillary block into the shape of a slope. It may be helpful to attach the safetycover for this purpose. Then screw the other end to the Filling Adapter UNFFront Side at the Lovis capillary block. See Fig. 19 - 2.178 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample3Fig. 19 - 2Lovis + DMA M + Xsample 452 - Connecting the hose betweenDMA M and LovisLovis outlet - Xsample sensor hose4. Lead the free end of the pre-assembled hose "BA" (sensor hose) of theXsample 352/452 (7) to the rear side and attach an adapter UNF-UNF (5).5. Connect one end of the hose 600x3x2 (4) to the Filling Adapter UNF BackSide Complete of the Lovis capillary block. Screw the other end into theadapter UNF-UNF (5).6. Fix the cable clip (6) on the master instrument’s casing approx. 30 mm belowthe upper edge.7. Fit the hose 600x3x2 (4) into the cable clip (6).Air hose8. Connect the air hose of the Xsample 352/452 (8) to the AIR connector of theDMA M.Waste hose9. Connect the waste hose (10) either to the optional safety waste container orto an other waste container. Refer to the Xsample 352/452 instructionmanual.Rinsing hose(s)10. Prepare and connect the rinsing hose(s) (9) and the solvent bottle(s)according to the Xsample 352/452 instruction manual.C72IB002EN-E 179

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsDrip plate - waste container11. Connect the drain hose (11) coming from the rear side of the drip plate (12)either to the waste container where you have connected already the wastehose or to a separate waste vessel and secure it against slipping off. As aseparate vessel you can use e.g. the waste vessel 500 mL (Mat. No. 6210)supplied with the DMA M/DSA M, see Fig. 19 - 17.Completing the installation12. Attach the magazine (1) and the safety cover (2) according to the Xsample352/452 instruction manual. See Fig. 19 - 3. Ensure that the edge protectionand the crush protection are mounted on the Xsample safety cover; seeChapter 17.2. When attaching the safety cover, lead the hose which isconnected between the density cell and the front side of the Lovis capillaryblock through the slit in the metal part of the safety cover (see arrow).21Fig. 19 - 3Lovis + DMA M + Xsample 352/452 complete13. To put the measuring assembly into operation, continue with Chapter 24.180 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample19.1.3 Low Volume SetupRequired equipment:• Low Volume Hose Set Lovis Xsample 352/452• Magazine for 6 mL vials 48 positions• Vials 6 mL polystyreneFor further details see the product description list (PDL) on the USB flash drivesupplied with your Lovis 2000 M/ME.Fig. 19 - 4Lovis + DMA M + Xsample 452 with low volume hoses, low volumemagazine and conical 6 mL PS vialsXsample 352: The 6 mL PS vials are not suitable for Xsample 352. For thisinstrument only the combination of low volume hoses and the standard 12 mLvials can be used.Xsample 452: You can use only the low volume hoses or these hoses incombination with the low volume magazine and the 6 mL PS vials.NOTICEDo not use the low volume magazine and vials if your samples chemicallyattack polystyrene (PS). In this case use the 12 mL glass vials together withthe standard 48 pos. magazine.To mount the low volume hoses, refer to Chapter 19.1.2. For assemblies withoutDMA M/DSA M see Chapter 18.1.2.Minimum required sample volume see Appendix A.4.C72IB002EN-E 181

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through Systems19.2 Lovis 2000 ME + DMA M + Xsample 52Required equipment:• Angular screwdriver Torx T20 (from Lovis accessories)• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)• Cutter or a pair of scissors19.2.1 Preparing Master Instrument and Modules1. Mount the Xsample 52 into the DMA M according to the Xsample 52instruction manual.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.5. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.19.2.2 Connecting the HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample filling and rinsing unit may only be connected by authorizedpersonnel who is fully trained to use the sample filling and rinsing unitcorrectly and safely.182 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample3277 89614 577approx.10 mmapprox.10 mmFig. 19 - 5Lovis + DMA M + Xsample 52 hose connectionsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" Xsample 52 Filling adapter for DMA M 122192 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis a density cell outlet to Lovis capillaryblock front side255233 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis4 Hose 300x3x2 PTFE 1x1/4"-28 UNF LovisLovis capillary block rear side to"Cell" connector on valve blockXsample 52sample filling hose; sample vessel -density cell inlet255241090475Hose Silicone 2x4 mml = approx. 200 mmXsample 52 air hose 512736 Hose 750x3x2 PTFE 1x1/4"-28 UNF Xsample 52 hose(s) for rinsing liquid(s) 212307 Cable clip self-adhesive Lovis hose guidance, 2 pcs. 62072a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 52(Mat. No. 101904).C72IB002EN-E 183

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through Systems1. Replace the currently installed injection adapters of the DMA M by theinjection adapters UNF (1) supplied with the Xsample 52. To do this refer tothe Xsample 52 instruction manual.2. Fix the cable clips (7) on the module housing ME as indicated in Fig. 19 - 5.Sample vessel - master instrument inlet3. Connect the hose 300x3x2 (4) to the inlet of the density cell. Bend itaccording to the shape and size of your sample vessel.TIPFor low viscous samples (below approx. 5 mPa.s), elevate the position of thesample vessel to improve the stability of the liquid column in the system. Thisleads to better repeatable results. If not connected to the drip plate, use e.g. thewaste vessel 500 mL supplied with the DMA M to place the sample vessel on. Ortake something similar with a height of approx. 10 cm, e.g. the box of a standardbottle. Best results can be achieved, if the bottom of the sample vessel is on thesame height as the connecting nozzles of the density meter (approx. 12.5 cm).See Fig. 19 - 6.Fig. 19 - 6Lovis + DMA M + Xsample 52: elevated sample vessel184 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleMaster instrument outlet - Lovis inlet4. Connect one end of the hose 530x3x2 (2) to the outlet of the density cell.Carefully bend the hose into the shape shown in Fig. 19 - 7. Screw the otherend to the Filling Adapter UNF Front Side of the Lovis capillary block. Fit thehose into the cable clip (7).72Fig. 19 - 7Lovis + DMA M + Xsample 52 - Mounting the hose between DMA Mand LovisLovis outlet - Xsample valve block5. Connect one end of the hose 600x3x2 (3) to the Filling Adapter UNF BackSide Complete of the Lovis capillary block. Screw the other end into the"Cell" connector on the Xsample 52 valve block, then fit the hose 600x3x2(3) into the cable clip (7).Air hose6. Cut a piece of approx. 200 mm from the Silicone hose 2x4 (5).7. Connect this hose to the AIR outlet of the DMA M and to the "AIR IN" nozzleof the Xsample 52 valve block.Rinsing hose(s)8. Prepare and connect the rinsing hose(s) (6) and the solvent bottle(s)according to the Xsample 52 instruction manual.C72IB002EN-E 185

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsDrip plate - waste container9. Connect the drain hose (8) coming from the rear side of the drip plate (9)either to the waste container where you have connected already the wastehose or to a separate waste vessel and secure it against slipping off. As aseparate vessel you can use e.g. the waste vessel 500 mL supplied with theDMA M, see Fig. 19 - 17.Completing the installation10. To put the measuring assembly into operation, continue with Chapter 24.19.3 Lovis 2000 ME + DMA M / DSA M + Xsample 122There are two filling options for sample changers with peristaltic pump:Filling by pressure: The sample goes first from the needle to the pump and thenfrom the pump to the instrument.Filling by suction: The sample goes first to the instrument and then through thepump.Required equipment:• Angular screwdriver Torx T20 (from Lovis accessories)• Cutter or a pair of scissors• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)19.3.1 Preparing Master Instrument and Modules1. Mount the Xsample 122 into the DMA M or DSA M according to theXsample 122 instruction manual.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Prepare the Xsample safety cover (edge protection, crush protection)according to Chapter 17.2.5. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.186 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample6. For assemblies with pressurized filling: Mount the cable clips on the rearside of the Module Housing Lovis ME according to Fig. 19 - 8 and all otheras described in the respective sections.approx. 45 mmapprox. 35 mmapprox. 110 mmFig. 19 - 8Mounting the cable clipsFor assemblies with filling by suction: Mount the cable clips as indicatedin the respective description.7. Connect the cables according to Chapter 6.6.8. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.19.3.2 Connecting the Standard HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample changer may only be connected by authorized personnel whois fully trained to use the sample changer correctly and safely.C72IB002EN-E 187

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through Systems19.3.2.1 Filling by PressureDMA M891DSA M31 27514137765543optionalwaste container:11911121016121516Fig. 19 - 9Lovis + DMA M/DSA M + Xsample 122, standard hoses; pressurizedfilling188 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a in-/outletDSA M: filling adapter density cellDMA M: filling adapter density celloutlet2 Injection adapter UNF 1/4" DSA Lovis b filling adapter sound velocity cellinlet3Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1224 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 122hose between needle and pumphoseconnector between Tygon hose andpump hose12219122146754067982Hose Silicone 2x4 mmXsample 122512735alternatively:pump hoseHose 2x4 Viton®Lovis54628l = approx. 250 mm6 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267397 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis8 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis10 Adapter UNF/UNF Lovispump hose to density cell inlet orsound velocity cell inletdensity cell outlet to Lovis capillaryblock front sideLovis capillary block rear side toadapter UNF/UNFconnects the hose 350 mm comingfrom Lovis capillary block rear sidewith the hose 600 mm to waste2554025523255256479011 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; adapter UNF/UNF towaste container2552412 Cable clip self-adhesive Lovis hose guidance 6207213Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081414 Adapter Luer cone Lovis tip for air hose 6386315Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130716 Waste container 8 L HDPE Optional waste container 105979a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).b. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).1. When using a DMA M: Replace the currently installed injection adapters ofthe DMA M by the injection adapters UNF (1). To do this refer to the Xsample122 instruction manual.C72IB002EN-E 189

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through Systems2. When using a DSA M: Replace the currently installed injection adapters byan injection adapter UNF (1) for the outlet of the density cell and an injectionadapter UNF 1/4" DSA (2) for the inlet of the sound velocity cell. To do thisrefer to the Xsample 122 and DSA M instruction manual.Needle - pump - master instrument3. Cut a piece of approx. 270 mm from the Tygon hose 1.6x3.2 (3).4. Connect the steel tube 2.1x2.4x20 mm (4) to the Tygon hose (3).5. Cut a piece of approx. 250 mm from the Silicone hose 2x4 (5). Alternatively,if you need a better chemical resistance, you can also use the Viton® hose2x4 supplied with the Lovis accessories.6. Plug the Silicone (or alternatively Viton®) hose (5) onto the free end of thesteel tube (4).7. Attach an adapter UNF/Hose (6) onto the free end of the Silicone (or Viton®)hose.8. Plug the Tygon end of this assembled hose onto the top of the needle.TIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.9. Turn the pump lever into horizontal position and remove the cartridge. Coilthe Silicone (or Viton®) part of this assembled hose around the peristalticpump. Attach the cartridge, hold it in place and pull the two hose endsslightly downwards, so that they are positioned in the groove of the cartridge.Then turn the pump lever to vertical position.10. Connect the hose 220x3x2 FEP 2x1/4"-28 (7) between the adapter UNF/hose (6) and the inlet of the density cell. When using a DSA M, connect thishose to the inlet of the sound velocity cell. Position the marker rings "C" thisway, that they fit into the hose holder on the Xsample 122. Secure the hoseby placing it into the hose holder.190 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleMaster instrument outlet - Lovis inlet11. Refer to Fig. 19 - 10: Connect one end of the hose 530x3x2 (8) to the outletof the density cell. Bend the hose at this connected end upwards and in frontof the Lovis capillary block into the shape of a loop. It may be helpful toattach the safety cover for this purpose (as shown in Fig. 19 - 12). Thenscrew the other end to the Filling Adapter UNF Front Side at the Loviscapillary block.8Fig. 19 - 10 Lovis + DMA M + Xsample 122 pressure filling - connecting the hosebetween Lovis and DMALovis outlet - waste container12. Connect the hose 350x3x2 FEP 2x1/4"-28 UNF (9) and the hose 600x3x2FEP 2x1/4"-28 UNF (11) with an adapter UNF/UNF (10).13. Screw the free end of the hose 350x3x2 into the Filling Adapter UNF BackSide Complete of the Lovis capillary block. Bend the hose as indicated inFig. 19 - 9 and secure it by placing the hose connectors with their springs inthe cable clips.14. Lead the free end of the hose 600x3x2 to a waste container and secure thehose against slipping off. When using the optional waste container 8 LHDPE, you can screw the connector of the hose directly into the screw capof this container.Drip plate - waste container15. Trim the hose (15) coming from the drip plate to the required length andconnect it either to the waste container or to a separate vessel and secure itagainst slipping off. As a separate vessel you can use e.g. the waste vesselsupplied with the DMA M, see Fig. 19 - 17.Air hose16. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (11). Attach anadapter Luer cone (12) onto one end.C72IB002EN-E 191

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through Systems17. Connect this hose to the AIR outlet of the DMA M or DSA M.Completing the installation18. Attach the magazine and the safety cover according to the Xsample 122instruction manual. Ensure that the edge protection and the crush protectionare mounted on the Xsample safety cover; see Chapter 17.2. Lead the hose,which is connected between the density cell and the front side of the Loviscapillary block, through the slit in the metal part of the safety cover. For anassembly with attached magazine and safety cover see Fig. 19 - 14.19. To put the measuring assembly into operation, continue with Chapter 24.192 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample19.3.2.2 Filling by Suction56DSA M491 2DMA M101181 3approx. 30 mm876Optionalwaste container:981214 81314Fig. 19 - 11 Lovis + DMA M/DSA M + Xsample 122, standard hoses; filling bysuctionC72IB002EN-E 193

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell outlet 122192 Injection adapter fitting DSA DSA Mfilling adapter sound velocity cellinlet122133 Injection adapter fitting Xsample 122 filling adapter density cell inlet 122314Hose Tygon 2375, 1.6x3.2 mm;approx. 270 mm5 Hose 530x3x2 FEP 2x1/4"-28 UNF LovisXsample 122filling hose, needle - density cellinlet (DMA M) or sound velocity cellinlet (DSA M)density cell outlet to Lovis capillaryblock front side67540255236 Hose 600x3x2 FEP 2x1/4"-28 UNF LovisLovis capillary block rear side -pump hose255247 Adapter UNF/hose (Female/Male) Lovis connector for filling hose 267398Hose Silicone 2x4 mm;alternatively:Hose 2x4 Viton®l = min. 600 mm but depends onwhere the waste container is placedXsample 122Lovispump and waste hose51273546289 Cable clip self-adhesive Lovis hose guidance 6207210Hose Silicone 3x5 mm l = approx. 200 mmLovis air hose 5081411 Adapter Luer cone Lovis tip for air hose 6386312 Adapter UNF/screwed tube13Hose Viton 5 x 8 withHose clamp Dmin 9.3, Dmax. 9.9length depends on where the wastecontainer is placedLovis;optional wastecontainerDrip plateto connect the pump hose to theoptional waste bottledrain hose from the drip plate18807725462130714 Waste container 8 L HDPE Optional waste container 10597915Tube d=2.1 D=2.4 L=20; 1.4571Lovisoptional use to split the Siliconehose 2x4 used as pump and wastehose.67982a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).1. When using a DMA M: Replace the currently installed injection adapters ofthe DMA M by an injection adapter UNF (1) for the outlet and an injectionadapter fitting (3) for the inlet of the density cell. Refer to the Xsample 122instruction manual.2. When using a DSA M: Replace the currently installed injection adapters byan injection adapter UNF (1) for the outlet of the density cell and an injectionadapter fitting DSA (2) for the inlet of the sound velocity cell. Refer to theXsample 122 and DSA M instruction manual.194 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleNeedle - master instrument3. Cut a piece of approx. 270 mm from the Tygon hose 1.6x3.2.4. Plug the Tygon hose (4) onto the needle top of Xsample 122.5. When using a DMA M: Connect the other end of the Tygon hose to theinjection adapter fitting on the DMA (3).When using a DSA M: Connect the other end of the Tygon hose to theinjection adapter fitting DSA (2).TIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.Master instrument - Lovis inlet6. Refer to Fig. 19 - 12: Connect one end of the hose 530x3x2 (5) to the outletof the density cell. Bend the hose at this connected end upwards and in frontof the Lovis capillary block into the shape of a loop. It may be helpful toattach the safety cover for this purpose. Then screw the other end to theFilling Adapter UNF Front Side at the Lovis capillary block.5Fig. 19 - 12 Lovis + DMA M + Xsample 122 suction filling - connecting the hosebetween Lovis and DMALovis outlet - pump - waste container7. Fix the cable clip (9) on the master instrument’s housing approx. 30 mmbelow the upper edge.8. Screw an adapter UNF/hose (7) onto one end of the hose 600x3x2 FEP 2x1/4-28" UNF (6). Connect the other end to the Filling Adapter UNF Back SideComplete of the Lovis capillary block.9. Cut a piece of min. 600 mm or the length you require to reach your wastecontainer from the Silicone hose 2x4 (8).C72IB002EN-E 195

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsTIPYou can split the Silicone hose 2x4 (8) into two parts of approx. 250 mm and thelength you require to reach the waste container. Connect the two pieces with atube d=2.1 D=2.4 L=20 (15). Advantages: easy replacement of the hose part usedin the peristaltic pump, less consumption of hose material. See Fig. 19 - 13.pump hose8to waste container815Fig. 19 - 13 Optional splitting the waste hose10. Plug one end of this hose (or if you have splitted to hose the free end of thehose with l = 250 mm) onto the adapter UNF/hose (7).11. Turn the pump lever to the horizontal position and remove the cartridge. Coilthe Silicone hose around the peristaltic pump as shown in Fig. 19 - 11.Attach the cartridge, hold it in place and pull the two hose ends slightlydownwards, so that they are positioned in the groove of the cartridge. Thenturn the pump lever to vertical position.12. Lead the free end of the Silicone hose to the waste container and secure itagainst slipping off. If you use the optional waste container 8 L HDPE (14): Plug the hose ontothe adapter UNF/screwed tube (12) on the screw cap of this container.13. Fit the hose 600x3x2 (6) into the cable clip (9).Drip plate - waste container14. Trim the hose (13) coming from the drip plate to the required length andconnect it either to the waste container or to a separate vessel and secure itagainst slipping off. As a separate vessel you can use e.g. the waste vessel500 mL supplied with the DMA M, see Fig. 19 - 17.Air hose15. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (10). Attach anadapter Luer cone (11) onto one end.16. Connect this hose to the AIR outlet of the DMA M / DSA M.196 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleCompleting the installation17. Attach the magazine (1) and the safety cover (2) according to theXsample 122 instruction manual. See Fig. 19 - 14. Ensure that the edgeprotection and the crush protection are mounted on the safety cover; seeChapter 17.2. Lead the hose, which is connected between the density celland the front side of the Lovis capillary block, through the slit in the metalpart of the safety cover. Avoid to squeeze or kink the hose.21Fig. 19 - 14 Lovis + DMA M + Xsample 122 complete18. To put the measuring assembly into operation, continue with Chapter 24.19.3.3 Connecting Chemically Resistant HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample changer may only be connected by authorized personnel whois fully trained to use the sample changer correctly and safely.Required equipment:• Retrofit kit for Xsample 122 Chemical (Mat. No. 96878) or• Retrofit kit for Xsample 122 Petro (Mat. No. 96880)• Xsample 122 instruction manual• Angular Screwdriver Torx T10 (supplied with the chemical retrofit kits and themodule housing ME)TIPIf your application requires an improved chemical resistance, a Lovis chemicalresistance upgrade set (Mat. No. 101899) is available. See Appendix B.3.C72IB002EN-E 197

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsRefer to the Xsample 122 instruction manual:Follow and adhere to all safety instructions and additional informations given insection "Installing the Optional Kit for Resistant Hoses.1. Switch off the master instrument.2. Replace the standard steel needle by the chemically resistant Hastelloyneedle according to the Xsample 122 instruction manual, section "Installingthe Optional Kit for Resistant Hoses - Exchanging the Needle".3. In case of retrofitting, remove all standard hoses except the hose connectedto the rear side of the Lovis capillary block.19.3.3.1 Filling by PressureConnect the hoses according to Fig. 19 - 15 and the table of parts below. Refer toChapter 19.3.2, further to the Xsample 122 and the DMA M / DSA M instructionmanual.198 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleDMA M:111041DSA M:3981 21516pump hose:5977cartridge:6568X1413optional waste container:11131418121718Fig. 19 - 15 Lovis + DMA M/DSA M + Xsample 122, chemical resistant hoses,pressurized fillingC72IB002EN-E 199

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Retrofit kitDMA M: filling adapter density cellin-/outletDSA M: filling adapter density celloutlet2 Injection adapter UNF 1/4" DSA Lovis a filling adapter sound velocity cellinlet3 Hastelloy needle Xsample 122 Retrofit kitchemically resistant needle withUNF thread, replaces the standardneedle1221912214924214 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis b needle to pump hose 255255Hose Norprene Chemical 3.2x6.4 (approx. 250 mm)orHose Norprene A-60-G 3.2x6.4 (approx. 250 mm)Retrofit kitpump hose, chemically resistant96878or968806 Adapter UNF/hose Retrofit kit connectors for the pump hose 267397 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kit8 Cartridge PVDF 3.2x6.4 Retrofit kit9 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis10 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis11 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis12 Adapter UNF/UNF Lovisto secure the pump hose on theadapter UNF/hosepump cartridge with large groove forchemical resistant hoses 3.2x6.4pump hose to density cell inlet(DMA M) or sound velocity cell inlet(DSA M)density cell outlet to flow-throughadapter on the Lovis capillary blockfront sideLovis capillary block rear side toadapter UNF/UNFconnects the hose 350 mm comingfrom Lovis capillary block rear sidewith the hose 600 mm to waste21531940862554025523255256479013 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; Lovis capillary blockrear side to waste container2552414 Cable clip self-adhesive Lovis hose guidance 6207215Hose Silicone 3x5 mmi = approx. 200 mmLovis air hose 5081416 Adapter Luer cone Lovis tip for air hose 6386317Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130718 Waste container 8 L HDPE Optional waste container 105979a. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).b. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).200 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample19.3.3.2 Filling by SuctionConnect the hoses according to Fig. 19 - 16 and the table of parts below. Referalso to Chapter 19.3.2, further to the Xsample 122 and the DMA M / DSA Minstruction manual.C72IB002EN-E 201

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsDMA M:5641DSA M:312101511161 2pump hose:78approx. 30 mm9988cartridge:147X1017optional waste container:181311131812Fig. 19 - 16 Lovis + DMA M/DSA M + Xsample 122, chemical resistant hoses,filling by suction202 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Retrofit kitDMA M: filling adapter density cellin-/outletDSA M: filling adapter density celloutlet2 Injection adapter UNF 1/4" DSA Lovis a filling adapter sound velocity cellinlet3 Hastelloy needle Xsample 122 Retrofit kitchemically resistant needle withUNF thread, replaces the standardneedleneedle to density cell inlet (DMA M)4 Hose 300x3x2 FEP 2x1/4"-28 UNF Retrofit kitor sound velocity cell inlet (DSA M)5 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis b density cell outlet to Lovis capillaryblock front side6 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis7Hose Norprene Chemical 3.2x6.4 l = approx. 250 mmorHose Norprene A-60-G 3.2x6.4 l = approx. 250 mmRetrofit kitLovis capillary block rear side topump hosepump hose, chemically resistant1221912214924213443255232552496878or968808 Adapter UNF/hose Retrofit kit connectors for the pump hose 267399 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kit10 Cartridge PVDF 3.2x6.4 Retrofit kit11 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis12 Adapter UNF/UNF Lovisto secure the pump hose on theadapter UNF/hosepump cartridge with large groove forchemical resistant hoses 3.2x6.4waste hose: pump hose to adapterUNF/UNFconnects the hose 350 mm comingfrom the pump hose with the hose600 mm to waste2153194086255256479013 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; adapter UNF/UNF towaste container2552414 Cable clip self-adhesive Lovis hose guidance 6207215Hose Silicone 3x5 mm l = approx. 200 mmLovis air hose 5081416 Adapter Luer cone Lovis tip for air hose 6386317Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130718 Waste container 8 L HDPE Optional waste container 105979a. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).b. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).C72IB002EN-E 203

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through Systems19.4 Lovis 2000 ME + DMA M /DSA M + Xsample 22There are two filling options for sample changers with peristaltic pump:Filling by pressure: The sample goes first from the needle to the pump and thenfrom the pump to the instrument.Filling by suction: The sample goes first to the instrument and then through thepump.Required equipment:• Angular screwdriver Torx T20 (from Lovis accessories)• Cutter or a pair of scissors• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)19.4.1 Preparing Master Instrument and Modules1. Mount the Xsample 22 into the DMA M or DSA M according to theXsample 22 instruction manual.2. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.3. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.4. Place the Module Housing Lovis ME onto the drip plate on top of the masterinstrument and check, whether it stands solidly on all four feet. Otherwiserefer to Chapter 6.5.5. Connect the cables according to Chapter 6.6.6. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.19.4.2 Connecting the Standard HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample filling unit may only be connected by authorized personnelwho is fully trained to use the sample filling unit correctly and safely.The standard hoses are supplied with the Xsample 22 and with theLovis 2000 ME accessories.204 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample19.4.2.1 Filling by PressureDMA M:8910101 2DSA M:1371 3111254waste vessel drain hose:(for example)76514approx. 30 mm1015approx. 10 mm10Fig. 19 - 17 Lovis + DMA M /DSA M + Xsample 22, standard hoses, pressurizedfillingC72IB002EN-E 205

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell outlet 122192 Injection adapter fitting Xsample 22 filling adapter density cell inlet 122313 Injection adapter fitting DSA DSA M4 Needle Xsample 225Hose Tygon 2375, 1.6x3.2 l = approx. 200 mmXsample 226 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 227Hose Silicone 2x4 mmalternatively:Hose 2x4 Viton®l = approx. 400 mmXsample 22Lovis8 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovisfilling adapter sound velocity cellinletsample filling needle, to be placed inthe sample vesselhose between needle and pumphoseconnector between Tygon hose andpump hosepump hose; between Tygon hoseand density cell inletdensity cell outlet to Lovis capillaryblock front side122136739467540679825127354628255239 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; Lovis capillary blockrear side to the waste container2552410 Cable clip self-adhesive Lovis hose guidance, 2 # 6207211Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081412 Adapter Luer cone Lovis tip for air hose 6386313 Waste bottle 1 L glass Optional waste vessel 10165514Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainer15 Waste vessel 500 mLDrip plate drain hose from drip plate 7254621307DMA M/DSA Mwaste vessel for drain hose 6210a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).1. When using a DMA M: Replace the currently installed injection adapters ofthe DMA M. Mount the injection adapter UNF (1) to the front connector of thedensity cell. Mount the injection adapter fitting (2) to the rear connector of thedensity cell. Refer to the Xsample 22 instruction manual.2. When using a DSA M: Replace the currently installed injection adapters ofthe DSA M. Mount the injection adapter UNF (1) to the front connector of thedensity cell. Mount the injection adapter fitting DSA (3) to the inlet of thesound velocity cell. Refer to the Xsample 22 and to the DSA M instructionmanual.3. Fix the cable clips (10) on the module housing ME and on the housing of themaster instrument as indicated in Fig. 19 - 17.206 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleNeedle - pump - master instrument4. Cut a piece of approx. 200 mm from the Tygon hose 1.6x3.2 (5).5. Attach one end of this hose to the needle (4).6. Connect the tube 2.1x2.4x20 mm (6) to the other end of the Tygon hose (5).TIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.7. Cut a piece of approx. 400 mm from the Silicone hose 2x4 (7).8. Connect the Silicone hose (7) to the free end of the tube 2.1x2.4x20 mm (6).9. Turn the pump lever to the horizontal position and remove the cartridge. Coilthe Silicone hose around the peristaltic pump as shown in Fig. 19 - 17.Attach the cartridge, hold it in place and pull the two hose ends slightlydownwards, so that they are positioned in the groove of the cartridge. Thenturn the pump lever to vertical position.10. Plug the free end of the Silicone (7) hose onto the injection adapter fitting (2)on the DMA M respectively to the injection adapter fitting DSA M (3).Master instrument outlet - Lovis inlet11. Refer to Fig. 19 - 18: Connect one end of the hose 530x3x2 (8) to the outletof the density cell. Bend the hose at this connected end upwards and in frontof the Lovis capillary block into the shape of a loop. Then screw the otherend to the Filling Adapter UNF Front Side at the Lovis capillary block. To holdthe hose in place, fit it into the cable clip (10) mounted on the modulehousing ME.810Fig. 19 - 18 Lovis + DMA M + Xsample 22 - connecting the hose between Lovisand DMAC72IB002EN-E 207

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsLovis outlet - waste vessel12. Connect the hose 600x3x2 FEP (9) to the Filling Adapter UNF Back SideComplete of the Lovis capillary block.13. Fit the hose 600x3x2 (9) into the cable clip (10) and lead it into the wastevessel. Secure the hose, to prevent it from slipping off. When using the optional waste bottle glass 1 L (13), you can connect thishose directly to the cap of this bottle.Drip plate - waste container14. Trim the hose (14) coming from the drip plate to the required length, connectit to a waste vessel (15; e.g. as indicated in Fig. 19 - 17) and secure itagainst slipping off.Air hose15. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (11). Attach anadapter Luer cone (12) onto one end.16. Connect this hose to the AIR outlet of the DMA M / DSA M.Completing the installation17. To put the measuring assembly into operation, continue with Chapter 24.208 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample19.4.2.2 Filling by SuctionDMA M:67101 2DSA M:5104141 3optional waste bottle:991311 12987waste vessel drain hose:(for example)1015approx. 10 mmapprox. 30 mm1610Fig. 19 - 19 Lovis + DMA M/DSA M + Xsample 22, standard hoses, filling bysuctionC72IB002EN-E 209

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell outlet 122192 Injection adapter fitting Xsample 22 filling adapter density cell inlet 122313 Injection adapter fitting DSA DSA4 Needle Xsample 225Hose Tygon 2375, 1.6x3.2 mm;approx. 200 mm6 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovisfilling adapter sound velocity cellinletsample filling needle, to be placed inthe sample vessel1221367394Xsample 22 needle to density cell inlet 67540outlet of density cell to Loviscapillary block front side255237 Hose 600x3x2 FEP 2x1/4"-28 UNF LovisLovis capillary block rear side topump hose255248 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 26739Hose Silicone 2x4Xsample 22512739alternatively:pump and waste hoseHose 2x4 Viton® Lovis54628approx. 600 mm10 Cable clip self-adhesive Lovis hose guidance, 2 # 6207211Hose Silicone 3x5 (approx. 200mm)Lovis air hose 5081412 Adapter Luer cone Lovis tip for air hose 6386313 Adapter UNF/screwed tube Lovis connector for waste hose 1880714 Waste bottle 1 L glass Optional waste vessel 10165515Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainer16 Waste vessel 500 mLDrip plateDMA M/DSA Mdrain hose from drip plate7254621307waste vessel for drain hose 6210a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).Refer to Fig. 19 - 19:1. When using a DMA M: Replace the currently installed injection adapters ofthe DMA M:Mount the injection adapter UNF (1) to the front connector of the density cell.Mount the injection adapter fitting (2) to the rear connector of the density cell.Refer to the Xsample 22 instruction manual.2. When using a DSA M: Replace the currently installed injection adapters ofthe DSA M. Mount the injection adapter UNF (1) to the front connector of the density cell.210 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleMount the injection adapter fitting DSA (3) to the inlet of the sound velocitycell. Refer to the Xsample 22 instruction manual and to the DSA Minstruction manual.3. Fix the cable clips (10) on the module housing ME and on the housing of themaster instrument as indicated in Fig. 19 - 19.Needle - master instrument inlet (sample suction hose)4. Cut a piece of approx. 200 mm from the Tygon hose 1.6x3.2 (5).5. Plug the Tygon hose (5) onto the needle (4). Connect the other end to theinjection adapter fitting (2) on the DMA M or to the injection adapter fittingDSA (3).TIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.Note: When using the chemical resistant hose set, this combination of needle andTygon hose is replaced by the hose 190x3x2 PTFE 1x1/4"-28 UNF as shown inFig. 19 - 22.Master instrument outlet - Lovis inlet6. Refer to Fig. 19 - 20: Connect one end of the hose 530x3x2 (6) to the outletof the density cell. Bend the hose at this connected end upwards and in frontof the Lovis capillary block into the shape of a loop. Then screw the otherend to the Filling Adapter UNF Front Side at the Lovis capillary block. To holdthe hose in place, fit it into the cable clip (10) mounted on the modulehousing ME.610Fig. 19 - 20 Lovis + DMA M + Xsample 22 - connecting the hose between Lovisand DMAC72IB002EN-E 211

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsLovis outlet - waste container7. Screw an adapter UNF/hose (8) onto one end of the hose 600x3x2 FEP 2x1/4-28" UNF (7). Connect the other end to the Filling Adapter UNF Back SideComplete of the Lovis capillary block.8. Cut a piece of approx. 600 mm from the Silicone hose 2x4 (9).9. Plug one end of the Silicone hose 2x4 onto the adapter UNF/hose (8).10. Turn the pump lever to the horizontal position and remove the cartridge. Coilthe Silicone hose around the peristaltic pump as shown in Fig. 19 - 19.Attach the cartridge, hold it in place and pull the two hose ends slightlydownwards, so that they are positioned in the groove of the cartridge. Thenturn the pump lever to vertical position.11. Lead the free end of the Silicone hose to the waste container.If you use the optional waste bottle glass 1 L (14): Screw an adapter UNF/screwed tube (13) into the insert of the bottle’s screw cap. Plug the hose (9)to this adapter.12. Fit the hose 600x3x2 (7) into the cable clip (10) mounted on the masterinstrument’s casing.Drip plate - waste container13. Trim the hose (15) coming from the drip plate to the required length, connectit to a waste vessel (16; e.g. as indicated in Fig. 19 - 19) and secure itagainst slipping off.Air hose14. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (11). Attach anadapter Luer cone (12) onto one end.15. Connect this hose to the AIR outlet of the DMA M / DSA M.Completing the installation16. To put the measuring assembly into operation, continue with Chapter 24.212 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + Xsample19.4.3 Connecting Chemically Resistant HosesWARNINGLiquids leaking from the instrument because of improper installation maycause injuries and risk of fire.• The sample filling unit may only be connected by authorized personnelwho is fully trained to use the sample filling unit correctly and safely.Required equipment:• Retrofit kit for Xsample 22 Chemical (Mat. No. 96879) or• Retrofit kit for Xsample 22 Petro (Mat. No. 96881)• Xsample 22 instruction manualTIPIf your application requires an improved chemical resistance, a Lovis chemicalresistance upgrade set (Mat. No. 101899) is available. See Appendix B.3.Refer to the Xsample 22 instruction manual:Follow and adhere to all safety instructions and additional informations given insection "Installing the Optional Kit for Resistant Hoses.1. Switch off the master instrument.2. In case of retrofitting, remove all standard hoses except the hose connectedto the rear side of the capillary block.19.4.3.1 Filling by PressureConnect the hoses according to Fig. 19 - 21 and the parts list below. Refer alsoto Chapter 19.4.2, further to the Xsample 22 and the DMA M /DSA M instructionmanual.C72IB002EN-E 213

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsDMA M:91011111DSA M:81331241 2pump hose:65465approx. 30 mm11approx. 10 mmwaste vessel drain hose:(for example)1114cartridge:7X15Fig. 19 - 21 Lovis + DMA M/DSA M + Xsample 22, chemical resistant hoses,pressurized filling214 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleNo. Item Supplied with Purpose Mat. No.DMA M filling adapter density cell1 Injection adapter UNF 1/4" DMA Retrofit kit12219in-/outlet2 Injection adapter UNF 1/4" DSA Lovis a filling adapter sound velocity cell12214inlet3 Hose 190x3x2 FEP 1x1/4"-28 UNF Retrofit kit sample vessel to pump hose 784534Hose Norprene Chemical 3.2x6.4 l = approx. 250 mmorHose Norprene A-60-G 3.2x6.4 l = approx. 250 mmRetrofit kitpump hose, chemically resistant96878or968805 Adapter UNF/hose Retrofit kit connectors for the pump hose 267396 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kitto secure the pump hose on theadapter UNF/hose215317 Cartridge PVDF 3.2x6.4 Retrofit kitpump cartridge with large groove forchemical resistant hoses 3.2x6.4940868 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis b pump hose to density cell inlet 255409 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to Lovis capillaryblock front side2552310 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; Lovis capillary blockrear side to the waste container2552411 Cable clip self-adhesive Lovis hose guidance, 2 # 6207212Hose Silicone 3x5 (approx. 200mm)Lovis air hose 5081413 Waste bottle 1 L glass Optional waste vessel 10165514Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainer15 Waste vessel 500 mLDrip plateDMA M/DSA Mdrain hose from drip plate7254621307waste vessel for drain hose 6210a. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).b. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).19.4.3.2 Filling by SuctionConnect the hoses according to Fig. 19 - 22 and the table of parts below. Referalso to Chapter 19.4.2, further to the Xsample 22 and the DMA M /DSA Minstruction manual.C72IB002EN-E 215

19 Lovis 2000 ME + DMA M / DSA M + Xsample SECTION II : Flow-Through SystemsDMA M:4 511101DSA M:119312131 2pump hose:6786786approx. 10 mm11waste vessel drain hose:(for example)approx. 30 mm1411cartridge:X159Fig. 19 - 22 Lovis + DMA M/DSA M + Xsample 22, chemical resistant hoses,filling by suction216 C72IB002EN-E

SECTION II : Flow-Through Systems19 Lovis 2000 ME + DMA M / DSA M + XsampleNo. Item Supplied with Purpose Mat. No.DMA M: filling adapter density cell1 Injection adapter UNF 1/4" DMA Retrofit kit12219in-/outlet2 Injection adapter UNF 1/4" DSA Lovis a filling adapter sound velocity cell12214inlet3 Hose 190x3x2 PTFE 1x1/4"-28 UNF Retrofit kit sample vessel to density cell inlet 784534 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis b density cell outlet to Lovis capillary25523block front side5 Hose 600x3x2 FEP 2x1/4"-28 UNF LovisLovis capillary block rear side topump hose255246 Adapter UNF/hose Retrofit kit connectors for the pump hose 267397Hose Norprene Chemical 3.2x6.4 l = approx. 250 mmorHose Norprene A-60-G 3.2x6.4 l = approx. 250 mmRetrofit kit8 Hose clip Dmin 5.8, Dmax 6.5 Retrofit kit9 Cartridge PVDF 3.2x6.4 Retrofit kitpump hose, chemically resistantto secure the pump hose on theadapter UNF/hosepump cartridge with large groove forchemical resistant hoses 3.2x6.496878or96880215319408610 Hose 600x3x2 FEP 2x1/4"-28 UNF Loviswaste hose; from pump hose towaste container2552411 Cable clip self-adhesive Lovis hose guidance, 2 # 6207212Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081413 Waste bottle 1 L glass Optional waste vessel 10165514Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainer15 Waste vessel 500 mLDrip plateDMA M/DSA Mdrain hose from drip plate7254621307waste vessel for drain hose 6210a. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).b. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905).C72IB002EN-E 217

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-20 Assemblies with DMA M,Alcolyzer ME, Haze QC ME, pH MEand XsampleSupported combinations:DMA 4500/5000 M + Xsample 22/122 + Alcolyzer Beer ME + HazeQC ME +Lovis 2000 ME + pH ME.Besides the full measuring assembly any subset of the above modules issupported.The measuring assembly can also be filled manually when using at least two20 mL syringes per sample.Fig. 20 - 1 shows a complete horizontal setup consisting of DMA M,Lovis 2000 ME, Alcolyzer ME, Haze QC ME, pH ME and Xsample 122, furtherthe optional Waste Container HDPE 8 L and the optional Stand For MeasuringAssembly. Such an assembly can be used e.g. for both beer and wortmeasurement.Fig. 20 - 1Assembly for beer and wort measurement218 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,NOTICEWhen retrofitting an existing DMA M/DSA M with a pH ME unit: Check yourmaster instrument. The pH ME module can only be used with instrumentswhich have a small removable cover on the rear side interface panel forinserting the electronic board of pH ME. If in doubt, contact your local AntonPaar distributor.CAN power supply: When setting up an assembly with external modules thatrequire more power than the master instrument can deliver (see technical data),a CAN power supply (Mat. No. 100655) is required to ensure proper power supplyand function of all components. Modules and instruments on the CAN chaininstalled before the CAN power supply are powered by the master, the rest ispowered by the CAN power supply. See Chapter 15.To achieve the best possible measuring results, consider the following tips:TIPS • Connect the hoses always as described for pressure filling. This reducesgenerally bubble formation, it is the best solution for setups with large heightusing several components placed one above the other. Or for setups with along liquid column due to many instruments in a line.• Xsample filling mode: Set the filling mode to "Forward only" (see yourXsample instruction manual). Filling "Forth and back" or "Forward with airgap" may lead to a bad filling quality in large setups with long liquid lines.• If applicable: The Alcolyzer ME must always be the first module after thedensity meter. Never place the Alcolyzer ME behind other modules in theline as this may lead to bad results.• If applicable: The pH ME module should always be the last in the linebecause of its cell geometry.20.1 Lovis 2000 ME + DMA M + Alcolyzer ME +HazeQC ME + pH ME + Xsample 122/22 - VerticalSetupRequired equipment:• Angular screwdriver Torx T20 (from Lovis accessories)• Cutter or a pair of scissors• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)C72IB002EN-E 219

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-20.1.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Mount the Xsample 122/22 into the DMA M according to the Xsample 122 orXsample 22 instruction manual.3. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.Arranging master instrument and modulesRefer to Fig. 20 - 2:4. Place the pH ME measuring cell unit (1) according to the pH ME instructionmanual.5. Attach the drip plate (6) onto the top of the master instrument and makesure, it is correctly engaged. See Chapter 17.3.6. Xsample 122 only: Prepare the Xsample safety cover (edge protection)according to Chapter 17.2.7. Place the Alcolyzer ME module (3) onto the drip plate on the right side of theDMA 4500/5000 (2; = master instrument).8. Place the Haze QC ME turbidity meter (4) onto the drip plate on the left sideof the master instrument.9. Place the Module Housing Lovis ME (5) on top of the two modules andcheck, whether it stands solidly on all four feet. Otherwise refer toChapter 6.5.220 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,543621Fig. 20 - 2Placing master instrument and modules - vertical setup1 pH ME2 DMA M3 Alcolyzer ME4 HazeQC ME5 Lovis 2000 ME6 Drip plateConnecting the cables10. Connect the cables according to Fig. 20 - 3C72IB002EN-E 221

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-.A41DB2E5C63Fig. 20 - 3Connecting the cables and the CAN power supply - vertical setupA Lovis 2000 MEB Haze QC MEC CAN power supply (Mat. No. 100655)D Alcolyzer MEE Master instrument1 CAN/Devicenet terminating resistor to CAN socket on Lovis ME2interface cable between Haze QC ME CAN socket and plug of CAN powersupplyinterface cable between Alcolyzer Beer ME CAN plug and socket of CAN3power supply4 interface cable between Alcolyzer ME CAN socket and Lovis ME CAN plug5 temperature sensor cable from pH ME6 cable of pH ME, to BNC connector of the master instrument222 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,Mounting the cable clips11. Fix the cable clips on the instrument housings according to Fig. 20 - 4.approx. 35 mmapprox. 15 mmapprox. 195 mmapprox. 15 mmapprox. 10 mmapprox. 10 mmmaster instrumentright side:clip matches withedgeFig. 20 - 4Positioning the cable clips (hose guidances)C72IB002EN-E 223

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-Attaching the capillary12. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.224 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,20.1.2 Connecting the Hoses - Vertical Setup10918 (6 #)87DMA M:1211221172241614 1520 191354 3 26Optionalwaste container:5 6 411 13 14172223232117Fig. 20 - 5Connecting the hoses - complete system, vertical setupC72IB002EN-E 225

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-No. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a DMA M: filling adapter density cell12219in-/outlet2Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1223 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1224Hose Silicone 2x4 mm, l = approx.250 mmXsample 122Lovishose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512735 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267396 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255407 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis8 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis10 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis11 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to Haze QC lowerconnectorHaze QC upper connector toAlcolyzer ME lower connectorAlcolyzer ME upper connector toLovis capillary block front sideLovis capillary block rear side toadapter UNF/UNF (direction to pHME)from adapter UNF/UNF to hose pHME255232554025524255242552512 Adapter UNF/UNF Lovisconnects the hose 600 mm comingfrom Lovis capillary block rear side 64790with the hose 350 mm13 Adapter UNF/Hose (Female/Male) Lovis connector for hose to pH ME 2673914Hose Silicone 2x4 mm, l = approx. 340 mmXsample 12215 Plug with compression fitting pH ME16 Jack with compression fitting pH ME17Hose PU 2.5x4 mm, length depends on position of wastecontainerpH MEfrom adapter UNF/Hose to the lowerconnector (inlet) of the pH MEmoduleto connect the filling hose to themeasuring cell jack (lowerconnector, inlet) of the pH moduleto connect the waste hose to themeasuring cell plug (upperconnector, outlet) of the pH modulewaste hose, from measuring cellplug of the pH module to the wastecontainer5127389234892331527218 Cable clip self-adhesive Lovis hose guidance 6207219Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081420 Adapter Luer cone Lovis tip for air hose 6386321 Adapter UNF/screwed tubeLovis;opt. wastecontainerto connect the waste hose to theoptional waste container18807226 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,No. Item Supplied with Purpose Mat. No.22Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130723 Waste container 8 L HDPE Optional waste container 10597924 Waste vessel 500 mL DMA Mwaste vessel for drain hose, if youdo not want to connect the drainhose to the large waste containera. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis Modular Assembly(Mat. No. 105880).62101. If required, replace the currently installed injection adapters of the DMA M bythe injection adapters UNF (1). Refer to the DMA M instruction manualrespectively to the instruction manual of your Xsample model. The procedureof dismounting/mounting is the same for all types of DMA M injectionadapters.For connecting the hoses refer to Fig. 20 - 5 and the detail pictures given withinthe text, further to the list of parts:Needle - pump - master instrument inlet64654432Fig. 20 - 6Assembling the hose between needle and inlet of master instrumentTIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.Refer to Fig. 20 - 6:2. Cut a piece of approx. 270 mm from the Tygon hose 1.6x3.2 (2).C72IB002EN-E 227

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-3. Connect the steel tube 2.1x2.4x20 mm (3) to the Tygon hose (2).TIPTo remove a Tygon hose from a tube, do not pull it. This will damage the hose andit cannot be re-used. Push the hose from the connector using your finger tipsrespectively your finger nails.4. Cut a piece of approx. 250 mm from the Silicone hose 2x4 (4).5. Plug the Silicone hose (4) onto the free end of the steel tube (2).6. Attach an adapter UNF/Hose (5) onto the free end of the Silicone hose (4).7. Carefully bend a hose 220x3x2 FEP 2x1/4"-28 (6) and connect it to theadapter UNF/hose (5).Refer to Fig. 20 - 5:8. Xsample 122: Plug the Tygon end (2) of this assembled hose onto the top ofthe needle.Xsample 22: Plug the Tygon end (2) of this assembled hose onto the steelneedle supplied with Xsample 22.9. Turn the pump lever into horizontal position and remove the cartridge. Coilthe Silicone part of this assembled hose around the peristaltic pump. Attachthe cartridge, hold it in place and pull the two hose ends slightly downwards,so that they are positioned in the groove of the cartridge. Then turn the pumplever to vertical position.10. Connect the free end of the hose 220x3x2 FEP 2x1/4"-28 (6) to the inlet ofthe density cell. Position the marker rings "C" this way, that they fit into thehose holder on the Xsample 122. Secure the hose by placing it into the hoseholder.Master instrument outlet - HazeQC ME - Alcolyzer ME inlet87Fig. 20 - 7Hoses master instrument outlet to Alcolyzer ME228 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,Refer to Fig. 20 - 7, see also the complete assembly in Fig. 20 - 5:11. Connect the hose 530x3x2 FEP 2x1/4"-28 (7) to the outlet of the density cell(see arrow).12. Carefully bend this hose into its shape as shown in Fig. 20 - 7, then connectit to the lower connector of HazeQC ME (inlet).13. Carefully bend the hose 220x3x2 FEP 2x1/4"-28 (8) into its shape andconnect it between the upper connector (outlet) of Haze QC ME and thelower connector (inlet) of the Alcolyzer ME.Alcolyzer ME outlet - Lovis 2000 ME inletRefer to Fig. 20 - 8:189Fig. 20 - 8Hose Alcolyzer ME to Lovis 2000 ME inlet14. Connect one end of the hose 600x3x2 FEP 2x1/4"-28 (9) to the upperconnector (outlet) of the Alcolyzer ME (see arrow in Fig. 20 - 7).15. Carefully bend the hose as indicated in Fig. 20 - 8. Then connect the freeend of this hose to the Lovis 2000 ME capillary block front side.16. Fit the hose into the two cable clips (18) to guide it and to minimize vibrationsof the hose when the capillary block is turning.Lovis 2000 ME outlet - pH ME inlet17. Connect one end of the hose 600x3x2 FEP 2x1/4"-28 (10) to theLovis 2000 ME rear side.18. Screw an adapter UNF/UNF (12) onto the free end of this hose and connectthe hose 350x3x2 FEP 2x1/4"-28 (11).C72IB002EN-E 229

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-19. Bend the hose 600x3x2 (10) according to Fig. 20 - 9 and fit it into the cableclips (18).1018121811Fig. 20 - 9Fitting the hose connected to Lovis 2000 ME rear side20. Place the adapter UNF/UNF (12) directly into the cable clip (18) mounted onthe Alcolyzer ME to fix the hose in place.TIPThe shape of the cable clips may vary. If a cable clip is too small to place theadapter UNF/UNF in it or the adapter cannot be held by the cable clip, place thespring of the hose connector into the cable clip.21. Cut a piece of l = approx. 340 mm from the Silicone hose 2x4 (14).22. Attach an adapter UNF/hose (13) onto the free end of this hose.23. Connect the silicone hose 2x4 to the FEP hose coming from Lovis 2000 MEby screwing the connector of the 350x3x2 FEP hose (11) into the adapterUNF/hose (12).24. Plug the free end of the silicone hose through the outer opening of thepH ME base plate.230 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,25. Attach the sleeve nut (15a) onto the silicone hose 2x4 (14). Fit the hose ontothe plug (15) and secure it with the sleeve nut. See Fig. 20 - 10. Thenconnect it to the inlet of the pH ME (lower connector).141515a14Fig. 20 - 10 Mounting the plug to the inlet hose for the pH ME modulepH ME - waste container26. Cut a piece of the required length to reach your waste container from thehose 2.5x4 PU (17).27. Mount the jack (16) to the PU hose in the same way as shown for the plug(15) in Fig. 20 - 10. Then connect it to the outlet of the pH ME (upperconnector).28. Lead the PU hose (17) to your waste vessel and secure it against slippingoff. When using the optional Waste Container HDPE 8 L - see Fig. 22 - 15and Fig. 22 - 16 - you can plug the free end of this hose directly to thiscontainer using an adapter UNF/screwed tube (21).Drip plate - waste container29. Trim the hose (22) coming from the drip plate to the required length andconnect it either to the waste container 8 L HDPE (23). Alternatively you canconnect it to a separate vessel, use e.g. the waste vessel 500 mL (24) or anyother suitable vessel. Secure the hose against slipping off.C72IB002EN-E 231

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-Air hose30. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (19). Attach anadapter Luer cone (20) onto one end.31. Connect this hose to the AIR outlet of the master instrument.Completing the installation32. For Xsample 122 only: Attach the magazine according to the Xsample 122instruction manual. Ensure that the edge protection is fixed on the Xsamplesafety cover; see Chapter 17.2. Lead the two hoses (hose between Alcolyzerand outlet of density cell; hose between Lovis capillary block rear side topH ME), through the slit in the metal part of the safety cover and attach thesafety cover (refer also to the instruction manual of Xsample 122). Avoid tosqueeze or kink the hoses.33. To put the measuring assembly into operation, continue with Chapter 24.20.2 Lovis 2000 ME + DMA M + Alcolyzer ME +HazeQC ME + pH ME + Xsample 122/22 -Horizontal SetupRequired equipment:• Angular screwdriver Torx T20 (from Lovis accessories)• Cutter or a pair of scissors• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)• Stand for measuring assembly 750x380x280 (Mat. No. 105451)20.2.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Mount the Xsample 122/22 into the DMA M according to the Xsample 122 orXsample 22 instruction manual.3. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.4. Xsample 122 only: Prepare the Xsample safety cover (edge protection)according to Chapter 17.2.232 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,Arranging master instrument and modules5. Place the stand for measuring assembly (1) onto the top of the masterinstrument (2) according to Fig. 20 - 11. The guides (3) on the right and rearside should touch the master instrument’s housing. This way the stand isheld correctly in position and the Xsample safety cover can be attachedeasily. If required, level the stand with the adjustable feet (open spanner10 mm).33Fig. 20 - 11 Positioning the stand for horizontal setup of a measuring assemblyRefer to Fig. 20 - 12:1 26. Place all modules onto the stand (2) as shown in Fig. 20 - 12. For pH MEmeasuring cell unit put the left front foot of Lovis 2000 ME into the openingon the base plate of pH ME. Refer also to the instruction manual of pH ME. Ifpossible, put your waste container under the stand besides the masterinstrument. When using the optional Waste Container HDPE 8 L, the wastehose can be directly plugged or screwed to this container.7. Check whether Lovis 2000 ME stands solidly on all four feet. Otherwise referto Chapter 6.5643521Fig. 20 - 12 Placing master instrument and modules - horizontal setupC72IB002EN-E 233

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-1 Master instrument2 Stand for measuring assembly3 Alcolyzer ME4 Haze QC ME5 Lovis 2000 ME6 pH MEConnecting the cables8. Connect the cables according to Fig. 20 - 13.BCAD1F526E73 4Fig. 20 - 13 Connecting the cables and the CAN power supply - horizontal setupA Alcolyzer MEB Haze QC MEC Lovis 2000 MED pH MEE CAN power supply (Mat. No. 100655)F Master instrument1 interface cable between master instrument and Alcolyzer ME (CAN plug)23interface cable between Alcolyzer ME (CAN socket) and CAN power supply(plug)interface cable between CAN power supply (socket) and HazeQC ME (CANplug)234 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,interface cable between HazeQC ME (CAN socket) and Lovis 2000 ME (CAN4plug)5 CAN/Devicenet terminating resistor on Lovis ME6 temperature sensor cable from pH ME7 cable of pH ME, to BNC connector of the master instrumentMounting the cable clips9. Mount the cable clips according to Fig. 20 - 14.Lovis 2000 MEapprox. 45 mmapprox. 35 mmapprox. 110 mmHazeQC MEapprox. 70 mmFig. 20 - 14 Mounting the cable clips on Lovis 2000 ME rear side and on HazeQCME - horizontal setupAttaching the capillary10. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.C72IB002EN-E 235

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-20.2.2 Connecting the HosesDMA M:1018 12 1818119198167211431317 14152019651098177Optionalwaste container:171421Fig. 20 - 15 Connecting the hoses - complete system, horizontal setup236 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,No. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a DMA M: filling adapter density cell12219in-/outlet2Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1223 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1224Hose Silicone 2x4 mm, l = approx.250 mmXsample 122Lovishose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512735 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267396 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255407 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis8 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis10 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis11 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to Alcolyzer MElower connectorAlcolyzer ME upper connector toHazeQC_ME lower connectorAlcolyzer ME upper connector toLovis capillary block front sideLovis capillary block rear side toadapter UNF/UNFadapter UNF/UNF to silicone hosefor pH ME255232554025523255252552512 Adapter UNF/UNF Lovisconnects the hose 350 mm comingfrom Lovis capillary block rear side 64790with the hose 350 mm to pH ME13 Adapter UNF/Hose (Female/Male) Lovis connector for hose to pH ME 2673914Hose Silicone 2x4 mm, l = approx. 340 mmLovis,Xsample 12215 Plug with compression fitting pH ME16 Jack with compression fitting pH ME17Hose PU 2.5x4 mm, length depends on position of wastecontainerpH MEfrom adapter UNF/Hose to the lowerconnector (inlet) of the pH MEmoduleto connect the filling hose to themeasuring cell jack (lowerconnector, inlet) of the pH moduleto connect the waste hose to themeasuring cell plug (upperconnector, outlet) of the pH modulewaste hose, from measuring cellplug of the pH module to the wastecontainer5127389234892331527218 Cable clip self-adhesive Lovis hose guidances, 3 pcs. 6207219Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081420 Adapter Luer cone Lovis tip for air hose 6386321 Waste container 8 L HDPE Optional waste container 105979a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis Modular Assembly(Mat. No. 105880).C72IB002EN-E 237

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-Needle - pump - master instrument inlet1. For Xsample 122: Prepare and connect the hose between needle and inlet ofthe density cell according to Chapter 20.1.2, steps 2 - 10, Fig. 20 - 6 andFig. 20 - 15. For Xsample 22: Plug the free Tygon end of this assembled hose to the steelneedle supplied with Xsample 22. Perform all other steps for this hose asgiven for Xsample 122.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.Master instrument outlet - Alcolyzer ME - HazeQC ME inlet2. Bend and connect the hose 530x3x2 FEP 2x1/4"-28 (7) between the outlet ofthe density cell and the Lovis 2000 ME front side. Refer to Fig. 20 - 16.87Fig. 20 - 16 Hoses between master instrument outlet Alcolyzer ME and HazeQCME outlet3. Carefully bend the hose 220x3x2 FEP 2x1/4"-28 (8) into its shape andconnect it between the upper connector (outlet) of Alcolyzer ME and thelower connector (inlet) of the Haze QC ME according to Fig. 20 - 16.HazeQC ME outlet - Lovis 2000 ME inlet4. Refer to Fig. 20 - 17: Carefully bend the hose 530x3x2 FEP 2x1/4"-28 (9)into its shape. Connect the hose to the upper connector of HazeQC ME(outlet), then place the hose in the cable clip (18). Now lead the hose inshape of the loop as shown and connect it to the Lovis 2000 ME capillaryblock front side.238 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,9189Fig. 20 - 17 Hose between HazeQC ME and Lovis 2000 MELovis 2000 ME outlet - pH ME inlet5. Prepare the hose between Lovis 2000 ME and HazeQC ME: Connect thetwo hoses 350x3x2 FEP 2x1/4"-28 (10, 11) using an adapter UNF/UNF (12).Connect this assembled hose to the Lovis 2000 ME rear side. Fix it inposition by putting the springs of the hose connectors into the cable clips(18) on the rear side of Lovis 2000 ME as shown in Fig. 20 - 18.18 1810 12 11Fig. 20 - 18 Assembling the hose between Lovis 2000 ME and Haze QC ME6. Cut a piece of l = approx. 340 mm from the Silicone hose 2x4 (14).7. Attach an adapter UNF/hose (13) onto the free end of this hose.8. Connect the silicone hose 2x4 to the FEP hose coming from Lovis 2000 MEby screwing the connector of the 350x3x2 FEP hose (12) into the adapterUNF/hose (13).9. Plug the free end of the silicone hose through the outer opening of thepH ME base plate.C72IB002EN-E 239

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-10. Attach the sleeve nut (15a) onto the silicone hose 2x4 (14). Fit the hose ontothe plug (15) and secure it with the sleeve nut. See Fig. 20 - 19. Thenconnect it to the inlet of the pH ME (lower connector).161117131514141515aFig. 20 - 19 Connecting the hoses to pH MEpH ME outlet - waste container11. Cut a piece of the required length to reach your waste container from thehose 2.5x4 PU (17).12. Mount the jack (16) to the PU hose (17) in the same way as shown for theplug (15) in Fig. 20 - 19. Then connect it to the outlet of the pH ME (upperconnector).13. Lead the PU hose (17) to your waste vessel and secure it against slippingoff. When using the optional Waste Container HDPE 8 L (21) - see alsoFig. 22 - 15 and Fig. 22 - 16 - you can plug the free end of this hose directlyto this container.Air hose14. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (19). Attach anadapter Luer cone (20) onto one end. Connect this hose to the AIR outlet ofthe master instrument. See Fig. 20 - 15.240 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,Completing the installation15. For Xsample 122 only: Attach the magazine according to the Xsample 122instruction manual. Ensure that the edge protection is fixed on the Xsamplesafety cover; see Chapter 17.2. Lead the hose between Alcolyzer ME andoutlet of density cell through the slit in the metal part of the safety cover andattach the safety cover (refer also to the instruction manual of Xsample 122).Avoid to squeeze or kink the hose.16. To put the measuring assembly into operation, continue with Chapter 24.20.3 Lovis 2000 ME + DMA M + Alcolyzer ME orHazeQC ME + pH ME + Xsample 122/22The setups and requirements are very similar to the complete system in horizontalsetup described in Chapter 20.2. So this chapter does not describe all steps indetails - refer to the respective sections as indicated.20.3.1 Preparing Master Instrument and Modules1. For preparation, arrangement of master instrument, stand and modules referto Chapter 20.2.1, further to Fig. 20 - 21.Connecting the cables2. Connect the cables according to Fig. 20 - 20.BAC13E42D56C72IB002EN-E 241

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-Fig. 20 - 20 Connecting the cables - DMA M + Lovis 2000 ME + HazeQC ME +pH ME + Xsample 122A Alcolyzer ME or HazeQC MEB Lovis 2000 MEC pH MED CAN power supply (Mat. No. 100655)E Master instrument12interface cable between master instrument and Alcolyzer ME or HazeQC ME(CAN plug)interface cable between Alcolyzer ME or HazeQC ME (CAN socket) and CANpower supply (plug)interface cable between CAN power supply (socket) and Lovis 2000 ME (CAN3plug)4 CAN/Devicenet terminating resistor on Lovis 2000 ME5 temperature sensor cable from pH ME6 cable of pH ME, to BNC connector of the master instrumentMounting the cable clips3. Mount the cable clips according to Chapter 20.2.1, step 8. Depending onyour setup, mount the single cable clip either on the housing of Haze QC MEor Alcolyzer ME.Attaching the capillary4. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.20.3.2 Connecting the HosesFig. 20 - 21 shows an assembly with Alcolyzer ME. The installation for anassembly with HazeQC ME is the identical procedure.242 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,DMA M: 91717 11 17108181572104312516 131419 1869816147Optionalwaste container:16181920Fig. 20 - 21 All hoses connected - DMA M + Lovis 2000 ME +Alcolyzer ME +pH ME + Xsample 122C72IB002EN-E 243

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-No. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a DMA M: filling adapter density cell12219in-/outlet2Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1223 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1224Hose Silicone 2x4 mm, l = approx.250 mmXsample 122Lovishose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512735 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267396 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255407 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis8 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis10 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to Alcolyzer ME orHazeQC ME lower connectorAlcolyzer ME or Haze QC ME upperconnector to Lovis capillary blockfront sideLovis capillary block rear side toadapter UNF/UNFadapter UNF/UNF to silicone hosefor pH ME2552325523255252552511 Adapter UNF/UNF Lovisconnects the hose 350 mm comingfrom Lovis capillary block rear side 64790with the hose 350 mm to pH ME12 Adapter UNF/Hose (Female/Male) Lovis connector for hose to pH ME 2673913Hose Silicone 2x4 mm, l = approx. 340 mmLovis,Xsample 12214 Plug with compression fitting pH ME15 Jack with compression fitting pH ME16Hose PU 2.5x4 mm, length depends on position of wastecontainerpH MEfrom adapter UNF/Hose to the lowerconnector (inlet) of the pH MEmoduleto connect the filling hose to themeasuring cell jack (lowerconnector, inlet) of the pH moduleto connect the waste hose to themeasuring cell plug (upperconnector, outlet) of the pH modulewaste hose, from measuring cellplug of the pH module to the wastecontainer5127389234892331527217 Cable clip self-adhesive Lovis hose guidances, 3 pcs. 6207218Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081419 Adapter Luer cone Lovis tip for air hose 6386320 Waste container 8 L HDPE Optional waste container 105979a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis Modular Assembly(Mat. No. 105880).244 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,Needle - pump - master instrument inlet1. For Xsample 122: Prepare and connect the hose between needle and inlet ofthe density cell according to Chapter 20.1.2, steps 2 - 10, Fig. 20 - 6 andFig. 20 - 21. For Xsample 22: Plug the free Tygon end of this assembled hose to the steelneedle supplied with Xsample 122. Perform all other steps for this hose asgiven for Xsample 122.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.Master instrument outlet - Alcolyzer ME or HazeQC ME inlet2. Bend and connect the hose 530x3x2 FEP 2x1/4"-28 between the outlet ofthe density cell and the Lovis 2000 ME front side. Refer to Fig. 20 - 16.Alcolyzer ME or HazeQC ME outlet - Lovis 2000 ME inlet3. Prepare and connect the hose according to Chapter 20.2.2, step 4.Lovis 2000 ME outlet - pH ME inlet4. Prepare and connect the hoses in the same way as described inChapter 20.2.2, steps 5 - 10. Refer also to Fig. 20 - 21 and the item list.pH ME outlet - waste container5. Prepare and connect the hose according to Chapter 20.2.2, steps 11 - 13.Air hose6. Prepare and connect the hose according to Chapter 20.2.2, step 14.Completing the installation7. For Xsample 122 only: Attach the magazine according to the Xsample 122instruction manual. Ensure that the edge protection is fixed on the Xsamplesafety cover; see Chapter 17.2. Lead the hose between Alcolyzer ME orHazeQC ME and outlet of density cell through the slit in the metal part of thesafety cover and attach the safety cover (refer also to the instruction manualof Xsample 122). Avoid to squeeze or kink the hose.8. To put the measuring assembly into operation, continue with Chapter 24.C72IB002EN-E 245

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-20.4 Lovis 2000 ME + DMA M + Alcolyzer ME orHazeQC ME + Xsample 122/22The setups and requirements are very similar to the complete system in horizontalsetup described in Chapter 20.2. So this chapter does not describe all steps indetails - refer to the respective sections as indicated.20.4.1 Preparing Master Instrument and Modules1. For preparation, arrangement of master instrument, stand and modules referto Chapter 20.2.1, further to Fig. 20 - 23.Connecting the cables2. Connect the cables according to Fig. 20 - 22.AB14D23CFig. 20 - 22 Connecting the cables - DMA M + Lovis 2000 ME + HazeQC ME +Xsample 122A Alcolyzer ME or HazeQC MEB Lovis 2000 MEC CAN power supply (Mat. No. 100655)D Master instrument12interface cable between master instrument and Alcolyzer ME or HazeQC ME(CAN plug)interface cable between Alcolyzer ME or HazeQC ME (CAN socket) and CANpower supply (plug)interface cable between CAN power supply (socket) and Lovis 2000 ME (CAN3plug)4 CAN/Devicenet terminating resistor on Lovis 2000 ME246 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,Mounting the cable clips3. Mount the cable clips according to Chapter 20.2.1, step 8. Depending onyour setup, mount the single cable clip either on the housing of Haze QC MEor Alcolyzer ME.Attaching the capillary4. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.20.4.2 Connecting the HosesFig. 20 - 21 shows an assembly with Alcolyzer ME. The installation for anassembly with HazeQC ME is the identical procedure.C72IB002EN-E 247

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-.DMA M:872112483514 136912 11121098Optionalwaste container:107131415Fig. 20 - 23 All hoses connected - DMA M + Lovis 2000 ME + Alcolyzer ME +Xsample 122248 C72IB002EN-E

SECTION II : Flow-Through Systems20 Assemblies with DMA M, Alcolyzer ME,No. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a DMA M: filling adapter density cell12219in-/outlet2Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1223 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1224Hose Silicone 2x4 mm, l = approx.250 mmXsample 122LovisNeedle - pump - master instrument inlethose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512735 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267396 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255407 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis8 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis10 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis11 Adapter UNF/UNF Lovisdensity cell outlet to Alcolyzer ME orHazeQC ME lower connectorAlcolyzer ME or HazeQC ME upperconnector to Lovis capillary blockfront sideLovis capillary block rear side toadapter UNF/UNFadapter UNF/UNF to wastecontainerconnects the hose 350 mm comingfrom Lovis capillary block rear sidewith the hose 600 mm to the wastecontainer255232552325525255246479012 Cable clip self-adhesive Lovis hose guidances, 3 pcs. 6207213Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081414 Adapter Luer cone Lovis tip for air hose 6386315 Waste container 8 L HDPE Optional waste container 105979a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis Modular Assembly(Mat. No. 105880).1. For Xsample 122: Prepare and connect the hose between needle and inlet ofthe density cell according to Chapter 20.1.2, steps 2 - 10, Fig. 20 - 6 andFig. 20 - 21. For Xsample 22: Plug the free Tygon end of this assembled hose to the steelneedle supplied with Xsample 22. Perform all other steps for this hose asgiven for Xsample 122.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.C72IB002EN-E 249

20 Assemblies with DMA M, Alcolyzer ME, Haze QC ME, pH ME and Xsample SECTION II : Flow-Master instrument outlet - Alcolyzer ME or HazeQC ME inlet2. Bend and connect the hose 530x3x2 FEP 2x1/4"-28 between the outlet ofthe density cell and the Lovis 2000 ME front side. Refer to Fig. 20 - 16.Alcolyzer ME or HazeQC ME outlet - Lovis 2000 ME inlet3. Prepare and connect the hose according to Chapter 20.2.2, step 4.Lovis 2000 ME outlet - waste container4. Connect a hose 350x3x2 FEP 2x1/4"-28 (9) and a hose 600x3x2 FEP 2x1/4"-28 (10) using an adapter UNF/UNF (11). Connect the free end of the hose350x3x2 to the Lovis capillary block rear side. Carefully bend the hose intoits shape and fix it by placing the springs of the hose connectors in the cableclips (12) on the rear side of the module housing ME.5. Lead the free end of the hose 600x3x2 (10) to the waste container andsecure it against slipping off. When using the optionalWaste Container HDPE 8 L (15), you can screw the free end of this hosedirectly to this container.912 11 1210Fig. 20 - 24 Connecting the hose between Lovis 2000 ME and waste containerAir hose6. Prepare and connect the hose according to Chapter 20.2.2, step 14.Completing the installation7. For Xsample 122 only: Attach the magazine according to the Xsample 122instruction manual. Ensure that the edge protection is fixed on the Xsamplesafety cover; see Chapter 17.2. Lead the hose between Alcolyzer ME orHazeQC ME and outlet of density cell through the slit in the metal part of thesafety cover and attach the safety cover (refer also to the instruction manualof Xsample 122). Avoid to squeeze or kink the hose.8. To put the measuring assembly into operation, continue with Chapter 24.250 C72IB002EN-E

SECTION II : Flow-Through Systems21 Assemblies with pH ME21 Assemblies with pH MESupported combinationsDMA 4100/4500/5000M, DSA 5000M + Lovis 2000 ME + pH ME + Xsample 122/22.Instead using a sample changer, the system can also be manually filled by syringe- min. two 10 mL syringes for sample displacement required.NOTICEWhen retrofitting an existing DMA M/DSA M: Check your master instrument.The pH ME module can only be used with instruments which have a smallremovable cover on the rear side interface panel for inserting the electronicboard of pH ME. If in doubt, contact your local Anton Paar distributor.21.1 DMA M/DSA M + Lovis 2000 ME + pH ME -Manually Filled by SyringeFor setting up a manually filled system, you have two options:• The pH ME module and the master instrument are connected, the Loviscapillary is filled separately.• The master instrument and all modules are connected with hoses. Thewhole system is filled with a syringe connected the master instrument. Thissetup is described in the following.Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)21.1.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Mount the Lovis 2000 ME into the DMA M/DSA M according to Chapter 6.4.3. Place the pH ME measuring cell unit according to the pH ME instructionmanual.4. Attach the cable clips onto the master instrument as indicated in Fig. 21 - 1.C72IB002EN-E 251

21 Assemblies with pH ME SECTION II : Flow-Through Systems5. Connect the cables according to Chapter 6.6.6. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.21.1.2 Connecting the HosesDMA M:2 18951211310104137 7a 55 649approx. inthe middleapprox. inthe middle141310 10Fig. 21 - 1Lovis ME + DMA M + pH - connecting the hoses252 C72IB002EN-E

SECTION II : Flow-Through Systems21 Assemblies with pH MENo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell outlet 122192 Injection adapter Luer DMA DMA M filling adapter density cell inlet 122253 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis b density cell outlet to Lovis capillaryblock front side255254 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis5Hose Silicone 2x4 mm, l = approx. 410 mm6 Adapter UNF/hose Lovis7 Plug with compression fitting pH ME8 Jack with compression fitting pH ME9Hose PU 2.5x4 mm, length depends on position of wastecontainerLovis capillary block rear side tofilling hose pH ME module25524Lovis from hose 600x3x2 to pH ME 51273pH MEconnects the hose 600x3x2 FEPand the hose silicone 410x2x4to connect the filling hose to themeasuring cell jack (lowerconnector, inlet) of the pH moduleto connect the waste hose to themeasuring cell plug (upperconnector, outlet) of the pH modulewaste hose, from measuring cellplug of the pH module to the wastecontainer2673989234892331527210 Cable clip self-adhesive Lovis hose guidance, 2 # 6207211Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081412 Adapter Luer cone Lovis tip for air hose 6386313 Waste bottle 1 L glass Optional waste vessel 101655a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set pH Option Lovis (Mat. No. 105880).b. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Flow-through filling set .Refer to Fig. 21 - 1:1. Replace the currently installed rear injection adapter of the DMA M by theinjection adapter UNF (1) supplied with Lovis 2000 ME. For replacement ofinjection adapters refer to the instruction manual of the DMA M. When using a DSA M: The injection adapter Luer for the DSA M must bemounted to the inlet of the sound velocity cell (the lower connector). Refer tothe instruction manual of the DSA M.2. If required, replace the currently installed front injection adapter of theDMA M by the injection adapter Luer DMA (2) supplied with this instrument.When using a DSA M: The injection adapter UNF for the DSA must bemounted on the outlet of the density cell (upper connector). Refer to theinstruction manual of the DSA M.C72IB002EN-E 253

21 Assemblies with pH ME SECTION II : Flow-Through SystemsMaster instrument outlet - Lovis inlet3. Refer to Fig. 21 - 2. Connect the hose 350x3x2 FEP (3) to the injectionadapter UNF (1) on the DMA M. Bend this hose into the shape of a loop.Now connect it to the Lovis capillary block front side.13Fig. 21 - 2Connecting the hose between DMA M and LovisLovis outlet - pH ME4. Connect the hose 600x3x2 FEP (4) to the Lovis capillary block rear side andbend it carefully as indicated.5. Cut a piece of l = 410 mm from the silicone hose 2x4 (5) and plug an adapterUNF/hose onto one end of this hose.6. Plug the free end of the silicone hose (5) through the outer opening of thepH ME base plate.7. Attach the sleeve nut (7a) onto the silicone hose 2x4 (5). Fit the hose ontothe plug (7) and secure it with the sleeve nut. See Fig. 21 - 1. Then connect itto the inlet of the pH ME (lower connector).pH ME - waste container8. Cut a piece of the required length to reach your waste container from thehose 2.5x4 PU (9).9. Mount the jack (8) to the PU hose in the same way as shown for the plug (7)in Fig. 21 - 1. Then connect it to the outlet of the pH ME (upper connector).10. Plug the free end of the PU hose through the inner opening of the pH MEbase plate. Fit the hose into the two cable clips mounted on the DMA M.Lead the hose to your waste vessel and secure it against slipping off.If you use the optional waste bottle glass 1 L (13): Screw an adapter UNF/screwed tube (14) into the insert of the bottle’s screw cap. Plug the hose (8)to this adapter.254 C72IB002EN-E

SECTION II : Flow-Through Systems21 Assemblies with pH MEAir hose11. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (11). Attach anadapter Luer cone (12) onto one end.12. Connect this hose to the AIR outlet of the master instrument.Completing the installation13. To put the measuring assembly into operation, continue with Chapter 24.21.2 DMA M/DSA M + Lovis 2000 ME + pH ME +Xsample 122/22Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Flow-through filling set (according to your order; further sets see the LovisProduct Description List on the USB flash drive supplied with your Lovis)21.2.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Mount the Xsample 122/22 into the DMA M according to the Xsample 122 orXsample 22 instruction manual.3. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.4. Place the pH ME measuring cell unit according to the pH ME instructionmanual.5. Attach the drip plate onto the top of the master instrument and make sure, itis correctly engaged. See Chapter 17.3.6. Place the Module Housing Lovis ME on top of the master instrument andcheck whether it stands solidly on all four feet. Otherwise refer toChapter 6.57. For Xsample 122 only: Prepare the Xsample safety cover (edge protection,crush protection) according to Chapter 17.2.8. Attach the cable clips as indicated in Fig. 21 - 5.C72IB002EN-E 255

21 Assemblies with pH ME SECTION II : Flow-Through Systems9. Connect the cables according to Fig. 21 - 3.51324Fig. 21 - 3Connecting the cables1 CAN/Devicenet terminating resistor to CAN socket on Lovis ME2 Interface cable between DMA M/DSA MCAN socket and Lovis ME CAN plug3 Temperature sensor cable from pH ME4 Cable of pH ME, to BNC connector of the master instrument5 Drip plate10. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.256 C72IB002EN-E

SECTION II : Flow-Through Systems21 Assemblies with pH ME21.2.2 Connecting the HosesFor a better filling performance and less bubble formation the hoses must beconnected for filling by pressure.Refer to Fig. 21 - 4:1. Replace the currently installed rear injection adapter of the DMA M by theinjection adapter UNF (1). For replacement of injection adapters refer to theinstruction manual of the DMA M. The procedure of dismounting/mounting isthe same for all types of DMA M injection adapters.When using a DSA M: The injection adapter Luer for the DSA M must bemounted to the inlet of the sound velocity cell (the lower connector). Refer tothe instruction manual of the DSA M. When using an Xsample 22: An injection adapter fitting for the DMA M/DSA M on the inlet of the density cell respectively the sound velocity cellmust be mounted. Refer to the instruction manual of Xsample 22 and of themaster instrument.C72IB002EN-E 257

21 Assemblies with pH ME SECTION II : Flow-Through SystemsDMA M:7 816141513161921621410111218179532 3 4916108waste vessel drain hose:(for example)65419waste container:approx. 30 mm15202116Fig. 21 - 4Lovis ME + DMA M + pH + Xsample 122 - connecting the hoses258 C72IB002EN-E

SECTION II : Flow-Through Systems21 Assemblies with pH MENo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell outlet and12219inlet2Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1223 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 122hose between needle and pumphoseconnector between Tygon hose andpump hose67540679824Hose Silicone 2x4 mm, l = approx.250 mmLovis pump hose 512735 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267396 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255407 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis8 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis10 Adapter UNF/UNF Lovis11Hose Silicone 2x4 mm, l = approx. 340 mmLovisdensity cell outlet to Lovis capillaryblock front sideLovis capillary block rear side tofilling hose pH ME modulebetween hose 600x3x2 coming fromLovis capillary block rear side andSilicone hose 2x4 to pH MEconnects the hose 600 mm comingfrom Lovis capillary block rear sidewith the hose 350 mmfrom adapter UNF/Hose to the lowerconnector (inlet) of the pH MEmodule255232552425525647905127312 Adapter UNF/hose (Female/Male) Lovis connector for hose to pH ME 2673913 Plug with compression fitting pH ME14 Jack with compression fitting pH ME15Hose PU 2.5x4 mm, length depends on position of wastecontainerpH MEto connect the filling hose to themeasuring cell jack (lowerconnector, inlet) of the pH moduleto connect the waste hose to themeasuring cell plug (upperconnector, outlet) of the pH modulewaste hose, from measuring cellplug of the pH module to the wastecontainer89234892331527216 Cable clip self-adhesive Lovis hose guidance 6207217Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081418 Adapter Luer cone Lovis tip for air hose 6386319Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip platedrain hose from drip plate725462130720 Waste container 8 L HDPE Optional waste container 10597921 Waste vessel 500 mLDMA M/DSA Mwaste vessel for drain hose, can beused alternatively to the large wastecontainera.Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905). When using a DSA M, these items can be found in the Hose Set Lovis+DSA+Xsample 22/122 (Mat.No. 102591).6210C72IB002EN-E 259

21 Assemblies with pH ME SECTION II : Flow-Through SystemsRefer to Fig. 21 - 4 and to the detail pictures in the text:Needle - pump - master instrument inlet1. Prepare and connect the hose between needle and the inlet of the densitycell of the DMA M (parts no. 2 - 6) in the (respectively the sound velocity cellfor a DSA M) according to Chapter 20.1.2, steps 2 - 10 and Fig. 20 - 6.When using an Xsample 22, refer also to Chapter 19.4.2.1.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.Master instrument outlet - Lovis inlet2. Bend the hose 530x3x2 FEP 2x1/4"-28 UNF (7) as indicated in Fig. 21 - 5.Connect it to the outlet of the density cell and then to the Lovis capillary blockfront side.7Fig. 21 - 5Connecting the hose between DMA M and Lovis 2000 MELovis outlet - pH ME3. Carefully bend the hose 600x3x2 FEP 2x1/4"-28 UNF as shown inChapter Fig. 21 - 6 and connect it to the Lovis capillary block rear side.this end to LovisFig. 21 - 6Preparing the hose 600x3x2260 C72IB002EN-E

SECTION II : Flow-Through Systems21 Assemblies with pH ME4. Screw an adapter UNF/UNF onto the free end of this hose and connect thehose 350x3x2 FEP 2x1/4"-28 UNF to this adapter.5. Prepare and connect the silicone hose 2x4 to the pH ME inlet and to thehose coming from the lovis capillary block rear side according toChapter 20.1.2, steps 21 - 25, Fig. 20 - 10 and Fig. 21 - 4.6. When this long hose between Lovis capillary block rear side and pH ME isreadily connected, place the hose 600x3x2 FEP into the cable clip mountedon the capillary block.pH ME - waste container7. Prepare and connect the waste hose to the outlet of the pH ME according toChapter 20.1.2, steps 26 - 28. Connect the other end to your wastecontainer. When using the optional Waste Container HDPE 8 L (19), you canplug the free end of this hose directly to this container.8. Place first the waste hose (15) in the cable clip (16) on the masterinstrument, then place the hose connector of the hose 350x3x2 (9) with itsspring into the cable clip to fix it in this position.91615Fig. 21 - 7Guiding the hosesAir hose9. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (19). Attach anadapter Luer cone (20) onto one end.10. Connect this hose to the AIR outlet of the master instrument.Drip plate - waste container11. Trim the hose (19) coming from the drip plate to the required length. Connectit e.g. to the waste vessel 500 mL (21) or any other suitable vessel. Securethe hose against slipping off. Or connect it to the same waste container asyour waste hose.C72IB002EN-E 261

21 Assemblies with pH ME SECTION II : Flow-Through SystemsCompleting the installation12. For Xsample 122 only: Attach the magazine according to the Xsample 122instruction manual. Ensure that the edge protection and the crush protectionare mounted on the safety cover; see Chapter 17.2. Lead the hoseconnected between the master instrument’s outlet and the Lovis capillaryblock front side through the slit in the metal part of the safety cover andattach the safety cover (refer also to the instruction manual of Xsample 122).Avoid to squeeze or kink the hose.13. To put the measuring assembly into operation, continue with Chapter 24.262 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat22 Assemblies with AbbematSupported combinations:Lovis 2000 ME + DMA 4100/4500/5000 M or DSA 5000 M + HazeQC ME +Abbemat 300/500/350/550 + pH ME + Xsample 22/122. Besides the fullmeasuring assembly any subset of the above modules is supported.Lovis 2000 ME + DMA 4100/4500/5000 M + Abbemat 300/500/350/550 +Xsample 352/452Lovis 2000 M +Xsample 22/122 + Abbemat 300/500/350/550The measuring assemblies can also be filled manually when using at least two20 mL syringes per sample (for sample displacement).Note:• Xsample 52 is not supported.• Abbemat models of the Heavy Duty line are not supportedNOTICEWhen retrofitting an existing DMA M with a pH ME unit: Check your masterinstrument. The pH ME module can only be used with instruments which havea small removable cover on the rear side interface panel for inserting theelectronic board of pH ME. If in doubt, contact your local Anton Paardistributor.CAN power supply: When setting up an assembly with external modules thatrequire more power than the master instrument can deliver (see technical data),a CAN power supply (Mat. No. 100655) is required to ensure proper power supplyand function of all components. Modules and instruments on the CAN chaininstalled before the CAN power supply are powered by the master, the rest ispowered by the CAN power supply. See Chapter 15.The Abbemat must also be connected both to the master instrument or to othermodules with a CAN cable and to the mains supply with its separate power supply.To achieve the best possible measuring results, consider the following tips:TIPS • Xsample 122/22: Connect the hoses always as described for pressure filling.This reduces generally bubble formation, it is the best solution for setupswith large height using several components placed one above the other. Orfor setups with a long liquid column due to many instruments in a line.• Xsample 122/22 filling mode: Set the filling mode to "Forward only" (see yourXsample instruction manual). Filling "Forth and back" or "Forward with airgap" may lead to a bad filling quality in large setups with long liquid lines.C72IB002EN-E 263

22 Assemblies with Abbemat SECTION II : Flow-Through Systems• If applicable: The HazeQC ME shall always be the first module after thedensity meter. Do not place the HazeQC ME behind other modules in theline as this may lead to bad results.• If applicable: The pH ME module should always be the last in the linebecause of its cell geometry.• Xsample 352/452: Never replace a low volume hose by a standard hose.22.1 Lovis 2000 M + Abbemat Manual Flow-ThroughFilling by SyringeTIPIf you need to save sample volume, you can fill the Lovis capillary and theAbbemat independent from each other. In this case you do not need theconnection kit Lovis-Abbemat flow-through.Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)• Connection kit Lovis-Abbemat flow-through (includes the micro flow cell andall required hoses and connectors)• Instruction Manuals for the master instrument and for all modules combinedin this measuring assembly22.1.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Mount the Lovis 2000 ME into the Lovis 2000 M Base Unit according toChapter 6.4.Mounting the micro flow cell3. Mount the micro flow cell to the Abbemat according to the Abbematinstruction manual, section "Micro Flow Cell".Arranging master instrument and modules4. Place the Abbemat on top of the Lovis 2000 M according to Chapter 22.2.1,step 4, Fig. 22 - 3.264 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematMounting the cable clips5. Mount the cable clips to the rear of Lovis 2000 M according toChapter 22.2.1, step 5, Fig. 22 - 1.Connecting the cablesConnect the cables according to Chapter 22.2.1, step 6, Fig. 22 - 2.Attaching the capillary6. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.C72IB002EN-E 265

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.1.2 Connecting the Hoses451279854109112 6 3 6Fig. 22 - 8Lovis 2000 M + Abbemat - All hoses connectedNote: Fig. 22 - 8 shows the assembly with optional drip plate on top of the Lovis2000 M. For manually filled assemblies this item is an option, for assembliesoperated with a sample changer it is a requirement. For more information seeChapter 17.3.266 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematNo. Item Supplied with Purpose Mat. No.1 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis a Lovis filling support to Lovis25525capillary block front side2 Hose 350x3x2 FEP 2x1/4"-28 UNF LovisLovis filling support - Lovis inlet1. Connect one end of the hose 350x3x2 FEP 2x1/4"-28 UNF (1) to the Lovisfilling support, the other end to the flow through adapter on the Loviscapillary block front side.Lovis outlet - Abbemat inletLovis capillary block rear side toadapter UNF/UNF255253 Adapter UNF/UNF Lovisto connect the hose 350x3x2 fromLovis and the hose 530x3x2 to 64790Abbemat4 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis Adapter UNF/UNF to Abbemat inlet 255235 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis Abbemat outlet to waste container 255246 Cable clip self-adhesive Lovis hose guidance 620727Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 508148 Adapter Luer cone Lovis tip for air hose 638639 Waste bottle 1 L glass Optional waste vessel 10165510Hose Viton 5x8with hose clamp Dmin 9.3 - Dmax9.9 mmlength depends on place of wastecontainerDrip plate(optional)drain hose from drip plate725462130711 Waste Bottle SVM 3000 0.5 L Lovis waste vessel for drain hose 17822a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Lovis Flow-Through Filling Set respectively inthe Connection Kit Lovis-Abbemat Flow-Through (Mat. No. 107879).2. Connect a hose 350x3x2 FEP 2x1/4"-28 (2) and a hose 530x3x2 FEP 2x1/4"-28 (4) using an adapter UNF/UNF (3). Connect the free end of the hose350x3x2 to the Lovis capillary block rear side. Carefully bend the hose intoits shape and fix it by placing the springs of the hose connectors in the cableclips (6) on the rear side of the Lovis 2000 M.3. Connect the free end of the hose 530x3x2 (4) to the inlet (= take one of thetwo connectors, preferably the right one seen from the front) of the Abbemat.C72IB002EN-E 267

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsAbbemat outlet - waste container4. Connect the hose 600x3x2 FEP 2x1/4"-28 (5) between the outlet of theAbbemat (= the remaining free connector) and your waste container. When using the optional waste bottle 1 L glass (9), you can connect the freeend of this hose directly to this bottle.Drip plate (option)5. When using the optional drip plate, refer to Chapter 21.2.2, step 11 and toFig. 21 - 4 for connecting the drain hose to the waste vessel.Air hose6. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (10). Attach anadapter Luer cone (11) onto one end.7. Connect this hose to the AIR outlet of the Lovis 2000 M.Completing the installation8. To put the measuring assembly into operation, continue with Chapter 24.22.2 Lovis 2000 ME + DMA M/DSA M + AbbematManually Filled by SyringeRequired equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)• Connection kit Lovis-Abbemat flow-through (includes the micro flow cell andall required hoses and connectors)• Instruction Manuals for the master instrument and for all modules combinedin this measuring assembly22.2.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Mount the Lovis 2000 ME into the DMA M/DSA M according to Chapter 6.4.Mounting the micro flow cell3. Mount the micro flow cell to the Abbemat according to the Abbematinstruction manual, section "Micro Flow Cell".268 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematArranging master instrument and modules4. Place the Abbemat on top of the DMA M/DSA M as shown in Fig. 22 - 3.Mounting the cable clips5. Mount the cable clips (9) to the rear of DMA M/DSA M according to Fig. 22 -1.approx. 45 mmapprox. 40 mm9 9Fig. 22 - 1Lovis 2000 M/DMA M/DSA M rear side - Mounting the cable clipsConnecting the cables6. Connect the cables according to Fig. 22 - 6.A132BFig. 22 - 2Lovis 2000 ME + DMA M + Abbemat - connecting the cables1 CAN/Devicenet terminating resistor to CAN socket on Abbemat2 interface cable between DMA M/DSA MCAN socket and Abbemat CAN plug3 mains cable AbbematA Abbemat (Performance/Performance Plus)B master instrument DMA M/DSA MC72IB002EN-E 269

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsNote: The Abbemat always needs power supply from the mains, independentfrom the CAN connection as it is an instrument, which can also be operated standalone.Attaching the capillary7. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.270 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat22.2.2 Connecting the HosesDMA M:7 82 1125DSA M:9 6 91 387541011Fig. 22 - 3Lovis 2000 ME + DMA M + Abbemat - All hoses connectedC72IB002EN-E 271

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell outlet 122192 Injection adapter Luer DMA DMA MRefer to Fig. 22 - 3:1. Replace the currently installed rear injection adapter of the DMA M by theinjection adapter UNF (1). For replacement of injection adapters refer to theinstruction manual of the DMA M. When using a DSA M: The injection adapter Luer for the DSA M (3) must bemounted to the inlet of the sound velocity cell (the lower connector). Refer tothe instruction manual of the DSA M.2. If required, replace the currently installed front injection adapter of theDMA M by the injection adapter Luer DMA (2) supplied with this instrument.When using a DSA M: The injection adapter UNF for the DSA (1) must bemounted on the outlet of the density cell (upper connector). Refer to theinstruction manual of the DSA M.Master instrument outlet - Lovis 2000 ME inletfilling adapter DMA M density cellinletfilling adapter DSA M sound velocity3 Injection adapter DSA (Luer) DSA Mcell4 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovis b density cell outlet to Lovis capillaryblock front side5 Hose 350x3x2 FEP 2x1/4"-28 UNF LovisLovis capillary block rear side toadapter UNF/UNF122251221125525255256 Adapter UNF/UNF Lovisto connect the hose 350x3x2 fromLovis and the hose 530x3x2 to 64790Abbemat7 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis Adapter UNF/UNF to Abbemat inlet 255238 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis Abbemat outlet to waste container 255249 Cable clip self-adhesive Lovis hose guidance, 2 # 6207210Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081411 Adapter Luer cone Lovis tip for air hose 6386312 Waste bottle 1 L glass Optional waste vessel 101655a. Lovis: Item can be found in the Lovis Accessory Kit Complete.b. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Lovis Flow-Through Filling Set respectively inthe Connection Kit Lovis-Abbemat Flow-Through (Mat. No. 107879).3. Refer to Fig. 22 - 4: Connect the hose 350x3x2 FEP (4) to the injectionadapter UNF (1) on the DMA M/DSA M. Bend this hose into the shape of aloop. Now connect it to the Lovis capillary block front side.272 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat14Fig. 22 - 4Connecting the hose between DMA M and LovisLovis outlet - Abbemat inlet4. Connect a hose 350x3x2 FEP 2x1/4"-28 (5) and a hose 530x3x2 FEP 2x1/4"-28 (7) using an adapter UNF/UNF (6). Connect the free end of the hose350x3x2 to the Lovis capillary block rear side. Carefully bend the hose intoits shape and fix it by placing the springs of the hose connectors in the cableclips (9) on the rear side of the master instrument.5. Connect the free end of the hose 530x3x2 (7) to the inlet (= take one of thetwo connectors, preferably to the right one seen from the front; see Fig. 22 -6) of the Abbemat.Abbemat outlet - waste container6. Connect the hose 600x3x2 FEP 2x1/4"-28 (8) between the outlet of theAbbemat (= the remaining free connector) and your waste container. When using the optional waste bottle 1 L glass (12), you can connect thefree end of this hose directly to this bottle.Air hose7. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (10). Attach anadapter Luer cone (11) onto one end.8. Connect this hose to the AIR outlet of the master instrument.Completing the installation9. To put the measuring assembly into operation, continue with Chapter 24.C72IB002EN-E 273

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.3 Lovis 2000 ME + DMA M/DSA M + Abbemat +Xsample 122/22Note: This assembly can also be set up with a Lovis 2000 M as masterinstrument.Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)• Connection kit Lovis-Abbemat flow-through (includes the micro flow cell andall required hoses and connectors)• Instruction Manuals for the master instrument and for all modules combinedin this measuring assembly22.3.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Mount the Xsample 122/22 into the master instrument according to theXsample 122 or Xsample 22 instruction manual.3. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.4. Prepare the Xsample safety cover (edge protection, crush protection)according to Chapter 17.2.Mounting the micro flow cell5. Mount the micro flow cell to the Abbemat according to the Abbematinstruction manual, section "Micro Flow Cell".Mounting the cable clips6. Attach the cable clips onto the rear side of the Module Housing Lovis ME asindicated in Fig. 22 - 8, upper part.Arranging master instrument and modules7. Place the stand onto the DMA M/DSA M or Lovis 2000 M according toFig. 20 - 11.8. Place the Lovis 2000 ME on the optional stand on top of the DMA M/DSA Mor Lovis 2000 M and check whether it stands solidly on all four feet.Otherwise refer to Chapter 6.5.274 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat9. Place the Abbemat on the left side of Lovis 2000 ME.10. If possible, you can put your own waste container into the free space underthe stand. Or use the optional Waste Container HDPE 8 L and place it under the standbesides the master instrument. This container supplies connectors to fix thewaste hose.Connecting the cables11. Connect the cables according to Fig. 22 - 5.BA132C 4Fig. 22 - 5Connecting the cables1 CAN/Devicenet terminating resistor to CAN socket on Abbemat2 interface cable between Lovis CAN socket and Abbemat CAN plug3 interface cable between DMA M/DSA MCAN socket and Lovis CAN plug4 mains cable AbbematA Abbemat (Performance/Performance Plus)B Lovis 2000 MEC master instrument DMA M/DSA M or Lovis 2000 MNote: The Abbemat always needs power supply from the mains, independentfrom the CAN connection as it is an instrument, which can also be operated standalone. An extra CAN power supply for this assembly is not required.Attaching the capillary12. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.C72IB002EN-E 275

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.3.2 Connecting the HosesDMA M:121182 1DSA M:16981 21211374514156optionalwaste container:12913121610111316Fig. 22 - 6Lovis 2000 ME + DMA M + Abbemat + Xsample 122 - all hosesconnected276 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell 122192 Injection adapter UNF 1/4" DSA Lovis b filling adapter sound velocity cellinlet122143Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1224 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1225Hose Silicone 2x4 mm, l = approx.250 mmXsample 122Lovishose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512736 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267397 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255408 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to Lovis capillaryblock front sideLovis capillary block rear side toadapter UNF/UNF255232552510 Adapter UNF/UNF Lovisconnects the hose 350 mm comingfrom Lovis capillary block rear side 64790with the hose 530 mm to Abbemat11 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis adapter UNF/UNF to Abbemat inlet 2552312 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovisoutlet of Abbemat to wastecontainer2552413 Cable clip self-adhesive Lovis hose guidances, 3 pcs. 6207214Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081415 Adapter Luer cone Lovis tip for air hose 6386316 Waste container 8 L HDPE Optional waste container 105979a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122(Mat. No. 101905) or in the Hose set Lovis+Xsample 22/122 (Mat. No. 101902), respectively in the Connection KitLovis-Abbemat Flow-Through (Mat. No. 107879).b. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).1. If required, replace the currently installed injection adapters of the DMA M bythe injection adapter UNF (1) supplied with Lovis 2000 ME. For replacementof injection adapters refer to the instruction manual of the DMA M. When using a DSA M: The injection adapter UNF for the DSA M must bemounted to the inlet of the sound velocity cell (the lower connector). Refer tothe instruction manual of the DSA M.Refer to Fig. 22 - 2 and to the detail pictures in the text:C72IB002EN-E 277

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsNeedle - pump - DMA M inlet (Lovis 2000 ME inlet)2. When using a DMA M with Xsample 122: Prepare and connect the hosebetween needle and the inlet of the density cell of the DMA M (parts no. 3 - 7in the above list) respectively the sound velocity cell for a DSA M accordingto Chapter 20.1.2, steps 2 - 10 and Fig. 20 - 6.When using a DMA M with Xsample 22, refer also to Chapter 19.4.2.1. When using a Lovis 2000 M with Xsample 122: Prepare and connect thehose between needle and the flow-through adapter of the Lovis according toChapter 18.3.2.1, steps 1 - 9, Fig. 18 - 4.When using a Lovis 2000 M with Xsample 22: Attach the hose clips andprepare and connect the hose according to Chapter 18.4.2.1, steps 2 - 9,Fig. 18 - 14.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.DMA M outlet - Lovis 2000 ME inlet3. Refer to Fig. 22 - 7: Connect one end of the hose 530x3x2 FEP 2x1/4"-28 (8)to the outlet of the density cell. Bend the hose at this connected end upwardsand in front of the Lovis capillary block into the shape of a loop. It may behelpful to attach the safety cover for this purpose (as shown in Fig. 19 - 12).Then screw the other end to the Filling Adapter UNF Front Side at the Loviscapillary block.8Fig. 22 - 7Connecting the hose between Lovis and DMA M278 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematLovis outlet - Abbemat inlet4. Connect a hose 350x3x2 FEP 2x1/4"-28 (9) and a hose 530x3x2 FEP 2x1/4"-28 (11) using an adapter UNF/UNF (10). Connect the free end of the hose350x3x2 to the Lovis capillary block rear side. Carefully bend the hose intoits shape and fix it by placing the springs of the hose connectors in the cableclips (13) on the rear side of the DMA M/DSA M.5. Connect the free end of the hose 530x3x2 (11) to the inlet of the Abbemat(=> take one of the two connectors, preferably to the right one seen from thefront, see Fig. 22 - 6).Abbemat outlet - waste container6. Connect the hose 600x3x2 FEP 2x1/4"-28 (12) between the outlet of theAbbemat (= the remaining free connector, see ) and your waste container. When using the optional Waste Container HDPE 8 L (16), you can connectthe free end of this hose directly to this container.Air hose7. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (14). Attach anadapter Luer cone (15) onto one end.8. Connect this hose to the AIR outlet of the master instrument.Completing the installation9. For Xsample 122 only: Attach the magazine and the safety cover accordingto the Xsample 122 instruction manual. Ensure that the edge protection andthe crush protection are mounted on the Xsample safety cover; seeChapter 17.2. When mounting the safety cover, guide the hose connectedbetween the master instrument’s outlet and the Lovis capillary block frontside through the slit in the metal part of the safety cover. Avoid to squeeze orkink the hose.10. To put the measuring assembly into operation, continue with Chapter 24.22.4 Lovis 2000 ME + DMA M/DSA M + HazeQC ME +Abbemat + Xsample 122/22Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)• Connection kit Lovis-Abbemat flow-through (includes the micro flow cell andall required hoses and connectors)• Instruction Manual Abbemat Performance/Performance Plus LineC72IB002EN-E 279

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsThis setup and its requirements are very similar to setups with Abbemat describedin Chapter 22.5 and Chapter 22.6 and other. So this chapter does not describe allsteps in details - refer to the respective sections as indicated.22.4.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Prepare the master instrument and modules and mount the micro flow cellaccording to Chapter 22.3.1, steps 2 - 5.Mounting the cable clips3. Mount the cable clips according to Fig. 22 - 8.Lovis 2000 MEapprox. 45 mmapprox. 35 mmapprox. 110 mmapprox. 20 mmapprox. in themiddle of thehousing’s heightat the edgeFig. 22 - 8Mounting the cable clips onto Module Housing Lovis ME280 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematArranging master instrument and modules4. Place the stand onto the DMA M/DSA M housing according to Fig. 20 - 11.Refer to Fig. 22 - 9:5. Place the Lovis 2000 ME Module Housing on the optional stand on top of theDMA M/DSA M and check whether it stands solidly on all four feet.Otherwise refer to Chapter 6.5.6. Place the other modules according to Fig. 22 - 9.7. If possible, you can put your own waste container into the free space underthe stand. Or place the optional Waste Container HDPE 8 L under the standbesides the master instrument. This container supplies connectors to fix thewaste hose.453261Fig. 22 - 9 Lovis 2000 ME + DMA M + HazeQC ME + Abbemat + Xsample 122- Placing master instruments and modules1 Master instrument DMA M/DSA M2 Optional stand for measuring assembly3 Lovis 2000 ME4 HazeQC ME5 Abbemat (Performance/Performance Plus)6 Optional waste container 8 L HDPEC72IB002EN-E 281

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsConnecting the cables8. Connect the cables according to Fig. 22 - 5.BA415C26DE3Fig. 22 - 10 Lovis 2000 ME + DMA M + HazeQC ME + Abbemat + Xsample 122- Connecting the cables1 CAN/Devicenet terminating resistor to CAN socket on Abbemat2 interface cable between Lovis CAN socket and Abbemat CAN plug3 interface cable between Lovis CAN plug and socket of CAN power supply4interface cable between plug of CAN power supply and CAN socket of HazeQCMEinterface cable between HazeQC ME CAN plug and DMA M/DSA MCAN5socket6 mains cable AbbematA Abbemat (Performance/Performance Plus)B HazeQC MEC Lovis 2000 MED master instrument DMA M/DSA ME CAN power supplyAttaching the capillary9. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.282 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat22.4.2 Connecting the HosesDMA M:141015111DSA M:1910161 215149161211163874171856optionalwaste container:1512151614191319Fig. 22 - 11 Lovis 2000 ME + DMA M + HazeQC ME + Abbemat + Xsample 122- all hoses connectedC72IB002EN-E 283

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell 122192 Injection adapter UNF 1/4" DSA Lovis b filling adapter sound velocity cellinlet122143Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1224 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1225Hose Silicone 2x4 mm, l = approx.250 mmXsample 122Lovishose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512736 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267397 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255408 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Adapter UNF/UNF Lovis10 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis11 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis12 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to adapter UNF/UNFconnects the hose 220x3x2 comingfrom DMA M/DSA M with the hose530x3x2 to HazeQC MEadapter UNF/UNF to HazeQC MElower connectorHazeQC ME upper connector toLovis capillary block front sideLovis capillary block rear side toadapter UNF/UNF255236479025540255242552513 Adapter UNF/UNF Lovisconnects the hose 350 mm comingfrom Lovis capillary block rear side 64790with the hose 530 mm to Abbemat14 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis adapter UNF/UNF to Abbemat inlet 2552315 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovisoutlet of Abbemat to wastecontainer2552416 Cable clip self-adhesive Lovis hose guidances, 4 pcs. 6207217Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081418 Adapter Luer cone Lovis tip for air hose 6386319 Waste container 8 L HDPE Optional waste container 105979a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+ DMA+Xsample 122/22 (Mat.No. 101905), or in the Hose Let Lovis + Xsample 122/22 (Mat. No. 101902) respectively in the Connection Kit Lovis-Abbemat Flow-Through (Mat. No. 107879).b. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).1. If required, replace the currently installed injection adapters of the DMA M bythe injection adapter UNF (1) supplied with Lovis 2000 ME. For replacementof injection adapters refer to the instruction manual of the DMA M. 284 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematWhen using a DSA M: The injection adapter UNF for the DSA M (2) must bemounted to the inlet of the sound velocity cell (the lower connector). Refer tothe instruction manual of the DSA M.Refer to Fig. 22 - 11 and to the detail pictures in the text:Needle - pump - master instrument inlet2. When using a DMA M with Xsample 122: Prepare and connect the hosebetween needle and the inlet of the density cell of the DMA M (parts no. 3 - 7in the above list) respectively the sound velocity cell for a DSA M accordingto Chapter 20.1.2, steps 2 - 10 and Fig. 20 - 6.When using a DMA M with Xsample 22, refer also to Chapter 19.4.2.1.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.Master instrument outlet - HazeQC ME inlet3. Prepare this hose: Connect the hose 530x3x2 (8) and the hose 220x3x2 (10)with an adapter UNF/UNF (9). Bend it into the shape as shown in Fig. 22 -12.1089Fig. 22 - 12 Preparing the hose between master instrument outlet andHazeQC ME inlet4. Connect the free end of the hose 530x3x2 (8) to the outlet of the DMA M/DSA M. If required corrects its shape as indicated in Fig. 22 - 13.5. Connect the free end of the hose 220x3x2 (10) to the lower connector ofHazeQC ME. See Fig. 22 - 13.C72IB002EN-E 285

22 Assemblies with Abbemat SECTION II : Flow-Through Systems6. To keep the hose in place, fit it into the cable clips (16) as shown in Fig. 22 -13:1016916118Fig. 22 - 13 Mounting the hoses between master instrument outlet, HazeQC MEand Lovis capillary block front sideHazeQC ME outlet - Lovis 2000 ME inlet7. Bend the hose 600x3x2 FEP 2x1/4"-28 (11) into its shape as shown inFig. 22 - 14. Then screw it into the HazeQC ME upper connector andconnect it to the Lovis capillary block front side as shown in Fig. 22 - 14.10this end to LovisFig. 22 - 14 Preparing the hose between HazeQC ME and Lovis capillary blockfront side286 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat8. Guide the hose by placing it into the cable clip (16). See Fig. 22 - 13.Lovis 2000 ME outlet - Abbemat inlet9. Connect the hose 350x3x2 FEP 2x1/4"-28 (12) and the hose 530x3x2 FEP2x1/4"-28 (14) with an adapter UNF/UNF (13).10. Bend the free end of the hose 350x3x2 (12) and connect it to the Loviscapillary block rear side.11. Connect the free end of the hose 530x3x2 (14) to the inlet of the Abbemat(=> take one of the two connectors, preferably to the right one seen from thefront; detail picture of Abbemat connectors see ).12. Fix the hose by placing it with the connector springs in the cable clips (16)mounted on the rear side of Lovis 2000 ME Module Housing.Abbemat outlet - waste container13. Connect the hose 600x3x2 FEP 2x1/4"-28 (15 between the outlet of theAbbemat (= the remaining free connector, see ) and your waste container. When using the optional Waste Container HDPE 8 L (19), you can connectthe free end of this hose directly to this container.Air hose14. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (17). Attach anadapter Luer cone (18) onto one end.15. Connect this hose to the AIR outlet of the master instrument.Completing the installation16. For Xsample 122 only: Attach the magazine and the safety cover accordingto the Xsample 122 instruction manual. Ensure that the edge protection andthe crush protection are mounted on the Xsample safety cover; seeChapter 17.2. When mounting the safety cover, guide the hose connectedbetween the master instrument’s outlet and the HazeQC ME through the slitin the metal part of the safety cover. Avoid to squeeze or kink the hose.17. To put the measuring assembly into operation, continue with Chapter 24.C72IB002EN-E 287

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.5 Lovis 2000 ME + DMA M/DSA M + Abbemat + pHME + Xsample 122/22Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)• Connection kit Lovis-Abbemat flow-through (includes the micro flow cell andall required hoses and connectors)• Hose set Lovis + DMA + Xsample 22/122• Instruction Manual Abbemat Performance/Performance Plus LineThis setup and its requirements are very similar to setups with Abbemat describedin Chapter 22.4, Chapter 22.6 and other. So this chapter does not describe allsteps in details - refer to the respective sections as indicated.22.5.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Prepare master instrument and modules and mount the micro flow cellaccording to Chapter 22.3.1, steps 2 - 5.Mounting the cable clips3. Attach the cable clips onto the rear side of the Module Housing Lovis ME asindicated in Fig. 22 - 8, upper part.Arranging master instrument and modules4. Place the stand onto the DMA M/DSA M housing according to Fig. 20 - 11.5. Place the Lovis 2000 ME Module Housing on the optional stand on top of theDMA M/DSA M and check whether it stands solidly on all four feet.Otherwise refer to Chapter 6.5.288 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat6. Place the other modules according to Fig. 22 - 15. For pH ME measuring cellunit put the left front foot of Lovis 2000 ME into the opening on the base plateof pH ME. Refer also to the instruction manual of pH ME.435261Fig. 22 - 15 Lovis 2000 ME + DMA M + pH ME + Abbemat + Xsample 122 -Placing master instruments and modules1 Master instrument DMA M/DSA M or Lovis 2000 M2 Optional stand for measuring assembly3 Lovis 2000 ME4 pH ME5 Abbemat (Performance/Performance Plus)6 Optional waste container 8 L HDPE7. If possible, you can put your own waste container into the free space underthe stand. Or use the optional Waste Container HDPE 8 L and place it under the standbesides the master instrument. This container supplies connectors to fix thewaste hose.C72IB002EN-E 289

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsConnecting the cables8. Connect the cables according to Fig. 22 - 16.CA31B46D25Fig. 22 - 16 Lovis 2000 ME + DMA M + Abbemat + pH ME + Xsample 122 -Connecting the cables1 CAN/Devicenet terminating resistor to CAN socket on Abbemat2 interface cable between Lovis CAN socket and Abbemat CAN plug3 interface cable between DMA M/DSA MCAN socket and Lovis CAN plug4 temperature sensor cable from pH ME5 cable of pH ME, to BNC connector of the master instrument6 mains cable AbbematA pH MEB Abbemat (Performance/Performance Plus)C Lovis 2000 MED master instrument DMA M/DSA M or Lovis 2000 MAttaching the capillary9. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.290 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat22.5.2 Connecting the HosesDMA M:12111DSA M:981 211 121331671920456121415179 11optionalwaste container:17 18102121Fig. 22 - 17 Lovis 2000 ME + DMA M + Abbemat + pH ME + Xsample 122 - Allhoses connectedC72IB002EN-E 291

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell 122192 Injection adapter UNF 1/4" DSA Lovis b filling adapter sound velocity cellinlet122143Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1224 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1225Hose Silicone 2x4 mm, l = approx.250 mmXsample 122Lovishose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512736 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267397 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255408 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to Lovis capillaryblock front sideLovis capillary block rear side toadapter UNF/UNF255232552510 Adapter UNF/UNF Lovisconnects the hose 350 mm comingfrom Lovis capillary block rear side 64790with the hose 530 mm to Abbemat11 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis adapter UNF/UNF to Abbemat inlet 2552312 Hose 350x3x2 FEP 2x1/4"-28 UNF LovisAbbemat outlet to adapter UNF/hose2552513 Adapter UNF/hose (Female/Male) Lovis connector for hose to pH ME 2673914Hose Silicone 2x4 mm, l = approx. 340 mmLovis15 Plug with compression fitting pH ME16 Jack with compression fitting pH ME17Hose PU 2.5x4 mm, length depends on position of wastecontainerpH MEfrom adapter UNF/Hose to the lowerconnector (inlet) of the pH MEmoduleto connect the filling hose to themeasuring cell jack (lowerconnector, inlet) of the pH moduleto connect the waste hose to themeasuring cell plug (upperconnector, outlet) of the pH modulewaste hose, from measuring cellplug of the pH module to the wastecontainer5127389234892331527218 Cable clip self-adhesive Lovis hose guidances, 4 pcs. 6207219Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081420 Adapter Luer cone Lovis tip for air hose 6386321 Waste container 8 L HDPE Optional waste container 105979a. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122 (Mat.No. 101905) or in the Hose Set Lovis + Xsample 122/22 (Mat. No. 101902) or in the Hose Set pH Option Lovis(Mat. No. 105880) or in the Connection Kit Lovis-Abbemat Flow-Through (Mat. No. 107879).b. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).292 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat1. If required, replace the currently installed injection adapters of the DMA M bythe injection adapter UNF (1). For replacement of injection adapters refer tothe instruction manual of the DMA M. When using a DSA M: The injection adapter UNF for the DSA M (2) must bemounted to the inlet of the sound velocity cell (the lower connector). Refer tothe instruction manual of the DSA M.Refer to Fig. 22 - 11 and to the detail pictures in the text:Needle - pump - DMA M inlet (Lovis 2000 ME inlet)2. When using a DMA M with Xsample 122: Prepare and connect the hosebetween needle and the inlet of the density cell of the DMA M (parts no. 3 - 7in the above list) respectively the sound velocity cell for a DSA M accordingto Chapter 20.1.2, steps 2 - 10 and Fig. 20 - 6When using a DMA M with Xsample 22, refer also to Chapter 19.4.2.1. When using a Lovis 2000 M with Xsample 122: Prepare and connect thehose between needle and the flow-through adapter of the Lovis according toChapter 18.3.2.1, steps 1 - 9, Fig. 18 - 4.When using a Lovis 2000 M with Xsample 22: Attach the hose clips andprepare and connect the hose according to Chapter 18.4.2.1, steps 2 - 9,Fig. 18 - 14.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.DMA M outlet - Lovis 2000 ME inlet3. Bend and connect the hose 530x3x2 FEP 2x1/4"-28 according toChapter 22.3.2, step3, Fig. 22 - 7.Lovis 2000 ME outlet - Abbemat inlet4. Prepare and connect this hose according to Chapter 22.3.2, steps 3 - 4.Abbemat outlet - pH MEFor steps 6 - 9 refer to Fig. 22 - 18.5. Cut a piece of l = approx. 340 mm from the Silicone hose 2x4 (14).6. Attach an adapter UNF/hose (13) onto the free end of this hose.7. Connect the silicone hose 2x4 to the FEP hose 350x3x2 FEP 2x1/4"-28 byscrewing the connector of the 350x3x2 FEP hose (12) into the adapter UNF/hose (13).C72IB002EN-E 293

22 Assemblies with Abbemat SECTION II : Flow-Through Systems8. Plug the free end of the silicone hose through the outer opening of thepH ME base plate.9. Attach the sleeve nut (15a) onto the silicone hose 2x4 (14). Fit the hose ontothe plug (15) and secure it with the sleeve nut. Then connect it to the inlet ofthe pH ME (lower connector).121113161415 17141515a12 13 14Fig. 22 - 18 Connecting the hoses between Abbemat and pH MEpH ME outlet - waste container10. Cut a piece of the required length to reach your waste container from thehose 2.5x4 PU (17).294 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat11. Mount the jack (16) to the PU hose (17) in the same way as shown for theplug (15) in Fig. 22 - 18. Then connect it to the outlet of the pH ME (upperconnector).12. Lead the PU hose (17) to your waste vessel and secure it against slippingoff. When using the optional waste container HDPE 8L (21), use the suppliedadapter UNF/screwed tube to connect the waste hose to this container.Air hose13. Cut a piece of approx. 200 mm from the Silicone hose 3x5 (19). Attach anadapter Luer cone (20) onto one end. Connect this hose to the AIR outlet ofthe master instrument. See Fig. 22 - 17.Completing the installation14. For Xsample 122 only: Attach the magazine and the safety cover accordingto the Xsample 122 instruction manual. Ensure that the edge protection andthe crush protection are mounted on the safety cover; see Chapter 17.2.When mounting the safety cover, guide the hose connected between themaster instrument’s outlet and the HazeQC ME through the slit in the metalpart of the safety cover. Avoid to squeeze or kink the hose.15. To put the measuring assembly into operation, continue with Chapter 24.C72IB002EN-E 295

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.6 Lovis 2000 ME + DMA M/DSA M + HazeQC ME +Abbemat + pH ME + Xsample 122/22Required equipment:• Angular screwdriver Torx T20 (from the Lovis accessories)• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)• Connection kit Lovis-Abbemat flow-through (includes the micro flow cell andall required hoses and connectors)• Hose set Lovis + DMA + Xsample 122/22 or Hose set Lovis + DSA +Xsample 122/22• Instruction Manual Abbemat Performance/Performance Plus LineThis setup and its requirements are very similar to setups with Abbemat describedin Chapter 22.5, Chapter 22.4 and other. So this chapter does not describe allsteps in details - refer to the respective sections as indicated.22.6.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (2.30 or higher) is installed on the master instrument. Thelatest version you will find on the USB flash drive supplied with Lovis 2000 M/ME. For the firmware version check and update refer to the instructionmanual of the master instrument.2. Prepare master instrument and modules and mount the micro flow cellaccording to Chapter 22.3.1, steps 2 - 5.Mounting the cable clips3. Attach the cable clips according to Chapter 22.4.1, Fig. 22 - 8.Arranging master instrument and modules4. Place the stand onto the DMA M/DSA M housing according to Fig. 20 - 11.5. Place the Lovis 2000 ME Module Housing on the optional stand on top of theDMA M/DSA M and check whether it stands solidly on all four feet.Otherwise refer to Chapter 6.5.6. Place all other modules according to Fig. 22 - 19. For pH ME measuring cellunit put the left front foot of Lovis 2000 ME into the opening on the base plateof pH ME. Refer also to the instruction manual of pH ME.7. If possible, you can put your own waste container into the free space underthe stand. Or place the optional Waste Container HDPE 8 L under the standbesides the master instrument. This container supplies connectors to fix thewaste hose.296 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat5463271Fig. 22 - 19 Lovis 2000 ME + DMA M + HazeQC ME + pH ME + Abbemat +Xsample 122 - Placing master instruments and modules1 Master instrument DMA M/DSA M or Lovis 2000 M2 Optional stand for measuring assembly3 Lovis 2000 ME4 HazeQC ME5 pH ME6 Abbemat (Performance/Performance Plus)7 Optional waste container 8 L HDPEConnecting the cablesConnect the cables between master instrument and Lovis 2000 ME, HazeQC MEand Abbemat according to Fig. 22 - 5.Connect the cables of pH ME according to Fig. 22 - 16.Attaching the capillary8. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.C72IB002EN-E 297

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.6.2 Connecting the HosesDMA M:1514111091DSA M:810211 214151692112111921387417 18optionalwaste container:20201222235614152116241320Fig. 22 - 20 Lovis 2000 ME + DMA M + Abbemat + pH ME + Xsample 122 - Allhoses connected298 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematNo. Item Supplied with Purpose Mat. No.1 Injection adapter UNF 1/4" DMA Lovis a filling adapter density cell 122192 Injection adapter UNF 1/4" DSA Lovis b filling adapter sound velocity cellinlet122143Hose Tygon 2375, 1.6x3.2 i = approx. 270 mmXsample 1224 Tube d=2.1 D=2.4 L=20; 1.4571 Xsample 1225Hose Silicone 2x4 mm, l = approx.250 mmXsample 122Lovishose between needle and pumphoseconnector between Tygon hose andpump hose6754067982pump hose 512736 Adapter UNF/Hose (Female/Male) Lovis connector for pump hose 267397 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis pump hose to density cell inlet 255408 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis9 Adapter UNF/UNF Lovis10 Hose 220x3x2 FEP 2x1/4"-28 UNF Lovis11 Hose 600x3x2 FEP 2x1/4"-28 UNF Lovis12 Hose 350x3x2 FEP 2x1/4"-28 UNF Lovisdensity cell outlet to adapter UNF/UNFconnects the hose 220x3x2 comingfrom DMA M/DSA M with the hose530x3x2 to HazeQC MEadapter UNF/UNF to HazeQC MElower connectorHazeQC ME upper connector toLovis capillary block front sideLovis capillary block rear side toadapter UNF/UNF255236479025540255242552513 Adapter UNF/UNF Lovisconnects the hose 350 mm comingfrom Lovis capillary block rear side 64790with the hose 530 mm to Abbemat14 Hose 530x3x2 FEP 2x1/4"-28 UNF Lovis adapter UNF/UNF to Abbemat inlet 2552315 Hose 350x3x2 FEP 2x1/4"-28 UNF LovisAbbemat outlet to adapter UNF/hose2552516 Adapter UNF/hose (Female/Male) Lovis connector for hose to pH ME 2673917Hose Silicone 2x4 mm, l = approx. 340 mmLovis18 Plug with compression fitting pH ME19 Jack with compression fitting pH ME20Hose PU 2.5x4 mm, length depends on position of wastecontainerpH MEfrom adapter UNF/Hose to the lowerconnector (inlet) of the pH MEmoduleto connect the filling hose to themeasuring cell jack (lowerconnector, inlet) of the pH moduleto connect the waste hose to themeasuring cell plug (upperconnector, outlet) of the pH modulewaste hose, from measuring cellplug of the pH module to the wastecontainer5127389234892331527221 Cable clip self-adhesive Lovis hose guidances, 4 pcs. 6207222Hose Silicone 3x5 mml = approx. 200 mmLovis air hose 5081423 Adapter Luer cone Lovis tip for air hose 6386324 Waste container 8 L HDPE Optional waste container 105979C72IB002EN-E 299

22 Assemblies with Abbemat SECTION II : Flow-Through Systemsa. Lovis: Item can be found in the Lovis Accessory Kit Complete or in the Hose Set Lovis+DMA+Xsample 22/122 (Mat.No. 101905) or in the Hose Set Lovis + Xsample 122/22 (Mat. No. 101902) or in the Hose Set pH Option Lovis(Mat. No. 105880) or in the Connection Kit Lovis-Abbemat Flow-Through (Mat. No. 107879).b. Lovis: DSA specific items can be found in the Lovis Accessory Kit Complete or in the Hose SetLovis+DSA+Xsample 22/122 (Mat. No. 102591).1. If required, replace the currently installed injection adapters of the DMA M bythe injection adapter UNF (1) supplied with Lovis 2000 ME. For replacementof injection adapters refer to the instruction manual of the DMA M. When using a DSA M: The injection adapter UNF for the DSA M (2) must bemounted to the inlet of the sound velocity cell (the lower connector). Refer tothe instruction manual of the DSA M.Refer to Fig. 22 - 20 and to the pictures indicated in the text:Needle - pump - master instrument inlet2. When using a DMA M/DSA M with Xsample 122: Prepare and connect thehose between needle and the inlet of the density cell of the DMA M (parts no.3 - 7 in the above list) respectively the sound velocity cell for a DSA Maccording to Chapter 20.1.2, steps 2 - 10 and Fig. 20 - 6.When using a DMA M/DSA M with Xsample 22, refer also toChapter 19.4.2.1.TIPIf your application requires chemically resistant hoses for the peristaltic pump,refer to Chapter 19.3.3.1 for Xsample 122 or to Chapter 19.4.3.1 for Xsample 22.Master instrument outlet - HazeQC ME inlet3. Prepare and connect the hose (parts No. 8 - 10 in the above list) according toChapter 22.4.2, steps 3 - 6, Fig. 22 - 13.HazeQC ME outlet - Lovis 2000 ME inlet4. Bend and connect the hose (part No. 11 in the above list) according toChapter 22.4.2, step 7, Fig. 22 - 13.Lovis 2000 ME outlet - Abbemat inlet5. Prepare and connect this hose (parts No. 12 - 14 in the above list) accordingto Chapter 22.3.2, steps 3 - 4.Abbemat outlet - pH ME inlet6. Prepare and connect the hose (parts No. 15 - 18 in the above list) accordingto Chapter 22.5.2, steps 5 - 9 and Fig. 22 - 18.300 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematpH ME outlet - waste container7. Prepare and connect the hose (parts No. 19 - 20 in the above list) accordingto Chapter 22.5.2,steps 10 - 12.Air hose8. Prepare and connect the air hose (parts No. 21 - 22 in the above list)according to Chapter 22.5.2, step 13.Completing the installation9. For Xsample 122 only: Attach the magazine and the safety cover accordingto the Xsample 122 instruction manual. Ensure that the edge protection andthe crush protection are mounted on the Xsample safety cover; seeChapter 17.2. When mounting the safety cover, guide the hose connectedbetween the master instrument’s outlet and the HazeQC ME through the slitin the metal part of the safety cover. Avoid to squeeze or kink the hose.10. To put the measuring assembly into operation, continue with Chapter 24.C72IB002EN-E 301

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.7 Lovis 2000 ME + DMA M + Abbemat +Xsample 352/452This measuring assembly can also be set up with a Lovis 2000 M as masterinstrument.Required equipment:• Lovis flow-through filling set (according to your order; further sets see theLovis Product Description List on the USB flash drive supplied with yourLovis)• Connection kit Lovis-Abbemat Xsample 352/452 (includes the micro flow celland all required low volume hoses and connectors)• Compressed air supply as specified in the Xsample instruction manual oroptionally an "Air preparation set", according to Chapter 23.1TIPFor the Xsample 452 also the low volume magazine with PS vials may beapplicable. Refer to Chapter 19.1.3 and to the Product Description List on theUSB flash drive supplied with your Lovis.NOTICE• This measuring assembly can only be used with low volume hoses andcompressed air supply for filling and drying the measuring system.• The measuring range of this assembly is limited to 500 mPa.s!Note: Low volume hoses have a smaller inner diameter (1 mm instead of 2 mm) thanthe standard hoses included in Lovis sets and accessory kits. The more viscousa sample is, the longer becomes the filling time and the greater is the risk of fillingproblems!22.7.1 Preparing Master Instrument and Modules1. Before setting up the measuring assembly, ensure that the latest instrumentfirmware version (V2.30 build 245 or higher) is installed on the masterinstrument. The latest version you will find on the USB flash drive suppliedwith Lovis 2000 M/ME. For the firmware version check and update refer tothe instruction manual of the master instrument.2. Mount the Xsample 352/452 into the DMA M according to the Xsample 352/452 instruction manual.3. Mount the Lovis 2000 ME into the Module Housing Lovis ME according toChapter 6.4.302 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with Abbemat4. Prepare the Xsample safety cover (edge protection, crush protection)according to Chapter 17.2.Mounting the micro flow cell5. Mount the micro flow cell to the Abbemat according to the Abbematinstruction manual, section "Micro Flow Cell".Mounting the cable clips6. Attach the 3 cable clips onto the rear side of the Module Housing Lovis MEas indicated in Fig. 22 - 21.approx. 45 mmapprox. 35 mmapprox. 110 mmlower left edgeFig. 22 - 21 Lovis 2000 ME rear side - Mounting the cable clipsArranging master instrument and modules7. Place the optional stand onto the DMA M according to Fig. 22 - 22.8. Place the Lovis 2000 ME Module Housing on the stand on top of the DMA Mand check whether it stands solidly on all four feet. Otherwise refer toChapter 6.5.9. Place the Abbemat on the left side of Lovis 2000 ME.C72IB002EN-E 303

22 Assemblies with Abbemat SECTION II : Flow-Through Systems3241Fig. 22 - 22 Lovis 2000 ME + DMA M + Abbemat + Xsample 352/452 - Placingmaster instrument and modules1 Master instrument DMA M with Xsample 352/4522 Lovis 2000 ME3 Abbemat (Performance/Performance Plus)4 Optional stand for measuring assembly304 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematConnecting the cables10. Connect the cables according to Fig. 22 - 23.AB132C4Fig. 22 - 23 Connecting the cables1 CAN/Devicenet Terminating resistor to CAN socket on Abbemat2 interface cable between Lovis CAN socket and Abbemat CAN plug3 interface cable between DMA M CAN socket and Lovis CAN plug4 mains cable AbbematA Abbemat (Performance/Performance Plus)B Lovis 2000 MEC master instrument DMA MNote: The Abbemat always needs power supply from the mains, independentfrom the CAN connection as it is an instrument, which can also be operated standalone. An extra CAN power supply for this assembly is not required.Attaching the capillary11. Attach the capillary with flow-through adapters to the Lovis according toChapter 9.5.C72IB002EN-E 305

22 Assemblies with Abbemat SECTION II : Flow-Through Systems22.7.2 Connecting the Hoses563214653628101112138 7 641471456Fig. 22 - 24 Lovis 2000 ME + DMA M + Abbemat + Xsample 452306 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematNo. Item Supplied with Purpose Mat. No.123456Injection adapterUNF 1/4" DMAHose 300x3x2 PTFE"AA"Hose 530x3x1 FEP2x1/4"-28 UNFHose 350x3x1 FEP2x1/4"-28 UNF "C"Hose 530x3x1 FEP2x1/4"-28 UNFHose 1000x3x1 FEP2x1/4"-28 UNF7 Adapter UNF/UNF8Hose 740x3x2 FEP2x1/4"-28 UNF "BA"9 Hose 2x4 Silicone1011121314Hose 750x3x2 PTFE1x1/4"-28 UNF "AD"Hose 2.5x4 PUtransparentbottle(s) for rinsingliquidHose 5x8 Viton-BKISO VersinicCable clip selfadhesiveXsample 352/452Connection kit Lovis-Abbemat Xsample 352/452Xsample 352/452Connection kit Lovis-Abbemat Xsample 352/452filling adapters density cell 12219hose between needle and DMA M 3443density cell outlet to Lovis capillaryblock front sideLovis capillary block rear side toadapter UNF/UNF75860109491adapter UNF/UNF to Abbemat inlet 75860Abbemat outlet to adapter UNF/UNFconnects the hose 350 mm comingfrom Lovis capillary block rear sidewith the hose 530 mm to Abbematand the hose 1000 mm coming fromAbbemat to Xsample 352/452sensor hose10949264790Sensor Hose Xsample 352/452 48722"Air IN" of the valve block to the "Air"nozzle of DMA M51273Hose(s) for rinsing liquids 21230Hose from the compressed airsupply to the "Pressure" connectorof the valve block15272solvent bottle(s) 21312Waste hose to a waste container a 72546hose guidances, 3 pcs. 62072a. Connect the hose to a customer’s waste container or to the optional safety waste containerOn Xsample 352/452 most hoses are pre-assembled. See the Xsample 352/452instruction manual.Needle - master instrument inlet1. Connect one end of the hose 300x3x2 PTFE (2) to the top of the Xsampleneedle, the other end to the inlet of the density cell. C72IB002EN-E 307

22 Assemblies with Abbemat SECTION II : Flow-Through SystemsMaster instrument outlet - Lovis 2000 ME inlet2. Connect one end of the hose 530x3x1 FEP 2x1/4"-28 UNF (3) to the outlet ofthe density cell. Bend the hose at this connected end upwards and in front ofthe Lovis capillary block into the shape of a slope (4). It may be helpful toattach the safety cover to verify whether the hose is positioned correctly.Then screw the other end to the Filling Adapter UNF Front Side at the Loviscapillary block. See Fig. 22 - 25.When using a Lovis 2000 M as master instrument, connect this hosebetween the top of the Xsample needle and the front of the capillary block.3Fig. 22 - 25 Lovis + Xsample 352/452: mounting the hose between needle andLovis inletLovis 2000 ME outlet - Abbemat inlet3. Connect a hose 350x3x1 FEP 2x1/4"-28 "C" (4) and a hose 530x3x1 FEP2x1/4"-28 (5) using an adapter UNF/UNF (7). Connect the free end of thehose 350x3x1 "C" to the Lovis capillary block rear side. Carefully bend thehose into its shape and fix it by placing the springs of the hose connectors inthe cable clips on the rear side of the Lovis 2000 ME.4. Connect the free end of the hose 530x3x1 to the inlet of the Abbemat (takeone of the two connectors, preferably the right one seen from the front).Abbemat outlet - Xsample sensor hose5. Lead the free end of the pre-assembled hose "BA" (8, sensor hose) of theXsample 352/452 to the rear side and attach an adapter UNF-UNF (7).6. Connect one end of the hose 1000x3x1 FEP 2x1/4"-28 (6) to the outlet of theAbbemat. Screw the other end into the adapter UNF-UNF of theXsample 352/452.7. Carefully bend the hose into its shape and fix it by placing the hose in thecable clip at the lower left edge on the rear side of the Lovis 2000 ME. 308 C72IB002EN-E

SECTION II : Flow-Through Systems22 Assemblies with AbbematAir hose8. Connect the air hose (9) of the Xsample 352/452 to the AIR connector (airpump outlet) of the DMA M master instrument.Compressed air9. Connect a hose either from your compressed air supply or the optional airpreparation set to the "Pressure" connector on the valve block (11). Furtherinformations see Chapter 23.NOTICEIf the pressure supplied to Xsample 352/452 is higher than 1.5 bar (21.7 psi),the valves in the valve block may not close correctly. This may causeXsample 452 to malfunction or be damaged. Only use compressed air withthese specifications: Maximum allowed pressure for incoming air: 1.5 bar(21.7 psi). The air must be dry and free of oil, water and dust.TIPIf you want to reduce the drying time significantly, we recommend using anoptional air preparation set with membrane air dryer, see Chapter 23.2respectively refer to the Lovis Product Description List on the USB flash drivesupplied with your Lovis.Rinsing hose(s)10. Prepare and connect the rinsing hose(s) (10) and the rinsing liquid bottle(s)(12) according to the Xsample 352/452 instruction manual.Waste hose11. Connect the waste hose (13) to the waste container according to theXsample 352/452 instruction manual.Completing the installation12. Attach the magazine according to the Xsample 352/452 instruction manual.13. Ensure that the edge protection and the crush protection are mounted on theXsample safety cover; see Chapter 17.2. Attach and use the safety coveraccording to the Xsample 352/452 instruction manual. Avoid to squeeze orkink the hose leading to the Lovis capillary block.14. To put the measuring assembly into operation, continue with Chapter 24.C72IB002EN-E 309

23 Compressed Air - Air Preparation Sets SECTION II : Flow-Through Systems23 Compressed Air - Air Preparation SetsXsample 352/452 can be operated (filling, cleaning) with compressed air. Themaximum air pressure must not exceed 1.5 bar / 0.15 MPa (21.7 psi) as theXsample valves then cannot switch correctly. Consult the Xsample 462/452instruction manual.For the measuring assembly DMA M + Lovis 2000 ME + Abbemat + Xsample352/452 it is required to use compressed air in combinations with one of thefollowing air preparation sets.23.1 Air Preparation Set Without DryerThis air preparation set is an accessory especially for Lovis 2000 M/ME withsample changers with magazine in the normal temperature range to increase thesample throughput by minimizing the drying times.NOTICEThe air preparation set without dryer shall not be used for measurementsbelow ambient temperature. Air humidity may condense in the system andlead to wrong readings or malfunctions.For such applications see Chapter 23.2.23.1.1 Parts of the Air Preparation Set6 5 412378Fig. 23 - 1 Air preparation set without air dryer (Mat. No. 21903)Parts of the air preparation set (without air dryer)1 Air filter 5 µm, AF40-F04 5 Manometer (pressure gauge) 0 - 0.2 MPa,G46-2-022 Mist filter 0.3 µm, AFM40-F04 6 Pressure hose 8x14; 3 m with connector3 Pressure regulator 0.85 MPa, AR40-F04H 7 Adapter Luer cone PTFE; 4 pcs.310 C72IB002EN-E

SECTION II : Flow-Through Systems23 Compressed Air - Air Preparation Sets4 Distributor AR40 OAJ00149 8 Hose 2.5x4 (PU transparent); 10 mThe kit includes also a set of fixing screws, washers and plugs.23.1.2 Mounting the Air Preparation SetParts see Fig. 23 - 1.1. Fix the air preparation unit (assembly of 1 - 6) e.g. to a wall aside or behindthe measuring assembly but not too far away from the instrument.2. Connect the pressure hose (6) to your compressed air supply.The outlet of your compressed air supply should be equipped with a stopcock,which allows to switch off the air preparation set easily.23.1.3 Connecting the Air HosesParts see Fig. 23 - 1 and Fig. 23 - 2.The distributor (4) is equipped with two adjustable outlets (4b) and a stop cock(4a). On each adjustable outlet you can set a continuous air flow, the stop cock isfor switching the air flow on and off (see Fig. 23 - 2). All outlets are equipped witha plug connector (4c). The two adjustable outlets provide regulating screws (4d)and locknuts (4e) to fix each setting.44b 4b 4aONOFF4e4c4dFig. 23 - 2Connecting air hoses to the distributor1. Cut a piece in your required length from the supplied air hose 2.5x4.C72IB002EN-E 311

23 Compressed Air - Air Preparation Sets SECTION II : Flow-Through Systems2. Connect this hose (8) between the stop cock (4a) on the distributor and tothe "Pressure" connector on the valve block of the Xsample 352/452.air preparation set w/o dryerto compressedair supply64a8"pressure" connectoron the distributor plateDMA M withX452/352Fig. 23 - 3 Connecting the pressure hose to Xsample 352/45223.1.4 Setting the Pressure on the Air Preparation UnitRefer to Fig. 23 - 4:1. Switch on your compressed air supply.70.10.150.05MPa55aFig. 23 - 4Setting the correct air pressure2. Reduce the incoming pressure:Therefore set the pressure regulator (5) to 1.5 bar / 0.15 MPa (21.7 psi). Thisis the maximum allowed pressure for the Xsample 352/452. To do this, pull the control knob (5a) downwards (you can see now a redmarking ring on the regulator) and turn it until the correct pressure can be312 C72IB002EN-E

SECTION II : Flow-Through Systems23 Compressed Air - Air Preparation Setsseen on the manometer (7). Then push the control knob upwards again tolock it in this position. The set pressure is now available on all three outletson the distributor.23.2 Air Preparation Set for Dew Point -40 °CUsing this air preparation set enables you to operate the measuring assembly inthe temperature range below ambient respectively at higher air humidity same asusual at "normal" conditions. It also allows to increase the sample throughput bydecreasing drying times.23.2.1 Parts of the Air Preparation Set9 8 7 6101112345131412Fig. 23 - 5 Air preparation set dew point -40 °C (Mat. No. 21902)Air preparation set (dew point -40 °C) - parts1 ... Air filter 5 µm, AF40-F04 8 ... Humidity indicator for membrane air dryer2 ... Mist filter 0.3 µm, AFM40-F04 9 ... Pressure hose 8x14; 3 m with connector3 ... Micro mist filter 0.1 µm, AFD40-F04 10 ... Drying cartridge4 ... Membrane air dryer -40°C, IDG30L-F03 11 ... Air hose (PU black) for drying cartridge5 ... Pressure regulator 0.85 MPa, AR40-F04H 12 ... T-plug connector QST 4; 2 pcs.6 ... Distributor AR40 OAJ00149 13 ... Hose 2.5x4 (PU transparent); 10 m7 ... Manometer (pressure gauge) 0 - 0.2 MPa,G46-2-0214 ... Adapter Luer cone PTFE; 4 pcs.The set includes also a set of fixing screws, washers and plugs.C72IB002EN-E 313

23 Compressed Air - Air Preparation Sets SECTION II : Flow-Through Systems23.2.2 Mounting the Air Preparation SetParts see Fig. 23 - 5.1. Fix the air preparation unit (assembly of 1 - 9) e.g. to a wall aside or behindthe measuring assembly but not too far away from the instrument.2. Connect the pressure hose (9) to your compressed air supply.The outlet of your compressed air supply should be equipped with a stop cock,which allows to switch off the air preparation set easily.23.2.3 Connecting the Air HosesThe distributor (6) is equipped with two adjustable outlets (6b) and a stop cock(6a). On each adjustable outlet you can set a continuous air flow, the stop cock isfor switching the Air flow on and off (see Fig. 23 - 6). All outlets are equipped witha plug connector (6c). The two adjustable outlets provide regulating screws (6d)and locknuts (6e) to fix each setting.66b 6b 6a ONOFF6e6c6dFig. 23 - 6 Connecting hoses to the distributor of the air preparation kit -40 °C1. Cut a piece in your required length from the supplied air hose 2.5x4.2. Connect this hose between the stop cock (6a) on the distributor and to the"Pressure" connector on the valve block of the XSample 352/452 accordingto Fig. 23 - 7.314 C72IB002EN-E

SECTION II : Flow-Through Systems23 Compressed Air - Air Preparation Setsair preparation set dew point -40 °Cto compressedair supply96b6a6b1011 12 13131313"pressure" connectoron the distributorplateDRY AIR IN:AIRPUMPINTERNALDMA M withX452(352)Fig. 23 - 7 Connecting pressure hose and drying cartridge to Xsample 352/4523. Connect a piece of hose 2.5x4 (13) between an adjustable outlet (6b) on thedistributor (6) and the DRY AIR IN - INTERNAL nozzle on the rear of theDMA M to keep the interior of the instrument (electronic boards) dry.4. Connect a piece of hose 2.5x4 (13) between an adjustable outlet (6b) on thedistributor (6) and the T-plug connector (12).5. Connect the black PU hose (11) to the drying cartridge (10). Plug the freeend of this hose to the T-plug connector (12).6. Connect a piece of hose 2.5x4 (13) between the T-plug connector (12) andthe DRY AIR IN - AIRPUMP nozzle on the rear of the DMA M.7. Remove the protection cap from the nozzle on the top of the drying cartridge.Why including the drying cartridge?When operating the Xsample 352/452 with compressed air, it mainly uses thecompressed air for filling sample and for cleaning purposes. But for some actionsduring measurement it uses also the built-in air pump, so it is required to keepalso this item dry. The dry air flow must not be connected directly to the DRY AIRIN - AIRPUMP nozzle, this causes pressure building up in the air pump, whichmay lead to malfunctions. With this setup the air pump is not pressure loaded, itworks with dried air and also the desiccant in the cartridge is kept dry.C72IB002EN-E 315

24 Putting a Measuring Assembly into Operation SECTION II : Flow-Through Systems24 Putting a Measuring Assembly intoOperationWorking with a measuring assembly is not the same as with just a singleinstrument. Each component can influence the other. Something that has noinfluence on one measurement may have a large influence on the determinationof another result. So especially filling and cleaning is a very important factor. Andthe different time each component may need for a valid result.Start-upAfter installing the hardware, switch on the master instrument.The power LED on the master instrument lights up green, the orange status LEDon the module housing Lovis ME blinks during the boot sequence.The master instrument shows the boot screen ("Loading Anton Paar Lovis ..." orLoading Anton Paar DMA M..."). During the start-up procedure, the masterinstrument checks for installed modules and should recognise them.When the boot sequence is completed, the master instrument displays the mainwindow. The status LED lights permanently.Check of instrument firmware versionCheck the firmware version of the master instrument and if required, perform afirmware update. This is required if• the installed module(s) is/are not recognized by the master instrument, e.g.in case of retrofitting a Lovis 2000 ME and a sample changer to an existingDMA M / DSA M - this requires Firmware Version 2.30 or higher. Normally this has to be performed before setting up the hardware of themeasuring assembly. If not done yet, the Lovis 2000 ME must be removed/disconnected before running the update.• the firmware of an installed part of the assembly is not up to date. In thiscase the master instrument prompts you to perform an update.The latest instrument firmware version at delivery time is supplied on a USB flashdrive in the accessory kit basic and in the accessory kit complete - Lovis.To check the firmware version and to perform a firmware update, see Chapter 14.Checking the system for leak tightnessTo ensure that all hoses and connectors are leak tight, perform the leak testaccording to Chapter 12.4.Selecting a MethodTap to open the list of methods.Select the desired method and tap .316 C72IB002EN-E

SECTION II : Flow-Through Systems24 Putting a Measuring Assembly into Operation• Lovis 2000 M/ME provides several predefined methods by default. SeeChapter 7.2.1.• When using a DMA M or DSA M as master instrument, consult theinstruction manual of your DMA M / DSA M for the description of methodsrelated to the density meter / sound velocity meter.• For the default settings of your sample changer model and for changingthese settings, refer to the Instruction Manual of the Xsample.Adapting method settingsFor many module combinations only default methods do exist. Select themeasuring quantities according to your application for screen display and datamemory.If you need other method settings for your application you can:• edit an existing method or• create a new methodTo edit an existing or create a new method, see Chapter 7.2 in SECTION I of thismanual. Refer also to the Instruction Manual of your DMA M/DSA M, if applicable.For all general information and instructions regarding the platform M firmwareconsult the Instrument Firmware Manual Platform M on the USB flash memorysupplied with your Lovis 2000 M/ME.Creating a capillary in the capillary administrationIf you start with a new system, you must create at least one capillary. Enter theserial number of the capillary, the capillary diameter, the ball type and a capillaryname in the menu "Setup > Measuring System Settings > Lovis Module >Capillary Administration". Define also, whether the capillary needs adjustmentor not. Refer to the Lovis 2000 M/ME Instruction Manual respectively toChapter 7.1.1.TIPIf you want to measure runtimes or relative viscosity only, it is not required toadjust the capillary.Conditioning the capillaryAll capillaries have undergone a cleaning procedure in the factory to removepossible residues from the production process. Anyway, to achieve better resultsit is recommended to clean the capillary with solvents suitable to your application.Use the supplied brushes to clean the inner surface of the capillary, then rinse anddry it. This way the wetting of the measuring surface with the sample will beimproved. See Chapter 11.2.2.C72IB002EN-E 317

24 Putting a Measuring Assembly into Operation SECTION II : Flow-Through SystemsPerforming a level adjustmentThe instruments cannot be levelled mechanically by adjustable feet. Tocompensate possible unevenness of the location of installation, perform a leveladjustment. Perform measurements and check the run times. A level adjustmentis required, if the obtained run times differ repeatedly between the forward run andthe backward run. The level adjustment can be performed using the samplechanger. See Chapter 25.1.Adjusting the capillaryIf you need viscosity results, it is required to adjust the capillary. To perform theadjustment using the sample changer see Chapter 25. Refer also to Chapter 8.4for single point, standard and extended adjustment in SECTION I of this manual.Adjustment data can also be imported from a USB flash drive.Handling measurement dataTo define data memory settings, data browser settings and data export settings,refer to the Instruction Manual of the master instrument.After performing all these steps, the measuring assembly should be ready.Adjusting other modules of an assemblyRefer to the instruction manual of the respective module. If required, consultadditionally the instruction manual of the master instrument.318 C72IB002EN-E

SECTION II : Flow-Through Systems25 Adjusting Lovis 2000 ME with Xsample25 Adjusting Lovis 2000 ME withXsampleThe following adjustments for the Lovis can be performed automated withXsample• Level Adjustment• Single Point Adjustment• Standard Adjustment• Extended AdjustmentDepending on your Xsample model, the steps you need to perform are different.Xsample 452/352:These models perform a special cleaning prior to starting the adjustment and acleaning procedure after the adjustment.Xsample 122/22: These models do not support automatic cleaning. Ensure that the measuring cellsand hoses of the system are sufficiently clean and dry before starting the leveladjustment.Xsample 52:This model uses the same hose for sample filling and as waste hose. Wastevessel and sample vessel have to be changed during the adjustment procedure.25.1 Level Adjustment with XsampleThe instruments cannot be levelled mechanically by adjustable feet, as they havenone. To compensate possible unevenness of the location of installation, performa Level Adjustment. This adjustment compensates differences between forwardand backward inclination of the Lovis 2000 M/ME caused by a not perfectlylevelled place of location. The Level Adjustment can correct an angle of max. 0.5°within one run. If the angle deviation is higher, the instrument recommends toimprove the Level Adjustment. Consult also Chapter 8.3 in SECTION I of thismanual.Perform a Level Adjustment• after first installation of the measuring assembly• when you have moved the Lovis 2000 ME or the master instrument from itslocation - even if only a bit• before adjusting a capillary. In this case, ideally perform the LevelAdjustment with the capillary you plan to adjust.NOTICEIt is not possible to perform a Level Adjustment with air, as the minimum runtime must not fall below 10 seconds. Further the friction in an empty capillarywill have negative influence on the run times.C72IB002EN-E 319

25 Adjusting Lovis 2000 ME with Xsample SECTION II : Flow-Through Systems1. Make sure that a capillary with ball is placed in the Lovis capillary block.2. Prepare a vial filled with appropriate reference medium.3. Select the menu "Checks / Adjustments > Other Adjustments > LovisModule > Level Adjustment".4. Put the vial into position 1 and tap .For Xsample 122/22 ensure that the system is clean and dry, as theseinstruments do not perform automatic cleaning.5. Follow the instructions given on the display.During the Level Adjustment Lovis 2000 M/ME displays the currenttemperature and the progress.When finished, the instrument shows the results for short and longmeasuring distance:• Required angle correction to achieve near identical runtimes• Average forward and backward runtimes• Forward/Backward Deviation and Variation CoefficientThe Variation Coefficient is shown for instrument firmware version 2.32 only.6. An arrow suggests the suitable action:• If the correction is greater than 0.5°, a message on the lower left-hand sideof the instrument screen reads "Check Result. Improve". The LevelAdjustment should be repeated with the same filling of the capillary. Tap . The first correction is applied and a new adjustment isstarted to fine tune the angle correction.• For corrections smaller than 0.5°, Lovis 2000 M/ME suggests to the Level Adjustment.• Tap to discard the displayed correction and end the adjustment.TIPThe result can be improved several times (even if Lovis 2000 M/ME suggests). It is possible to achieve angle corrections < 0.1°.7. If after improving several times Lovis does not allow you to , checkthe filling of the capillary for bubbles and check that the Lovis stands in astable position. Refill the capillary and tap .8. The Level Adjustment is valid and stored after tapping .25.2 Capillary Adjustment with XsampleAll capillary adjustments for Lovis 2000 ME can be performed using the samplechanger. To perform the adjustment, you need a reference standard with certifiedvalues of dynamic viscosity and density at the respective temperature. Theviscosity of the reference standard must be in the core range of the capillary/ballconfiguration, see Appendix A.2.320 C72IB002EN-E

SECTION II : Flow-Through Systems25 Adjusting Lovis 2000 ME with XsampleThe sample changers perform the adjustment procedure in a different way. Followthe instructions on the display and consult also the instruction manual of yourXsample model, Chapter "Adjusting the Master Instrument with Xsample xxx".TIPIf you have ordered a factory adjustment, import the adjustment from the USBflash drive delivered with the capillary. See Chapter 7.1.1.Do not lose the original adjustment ball. See Chapter 8.4!1. Ensure that:• the capillary data are entered in the Capillary Administration. If not donealready, refer to Chapter 7.1.1 in SECTION I of this manual.• the capillary you want to adjust is in the Lovis capillary block.• the solvent bottles for Xsample 352/452/52 contain enough suitablesolvent.2. Prepare a vial filled with the reference standard.3. Select the desired adjustment type in the menu "Checks / Adjustments >Other Adjustments > Lovis Module":• "Single Point Adjustment" –> for one user-defined angle• "Standard Adjustment" –> for a range of 20° to 70° angle• "Extended Adjustment" –> for a range of 15° to 80° anglePreferably use Single Point Adjustment or Standard Adjustment. Only do anExtended Adjustment if you really need to measure at angles 70°!4. Enter the requested parameters and data and follow all instructions given onthe screen.TIPYou get the most precise measuring results if adjusting a capillary at or near thattemperature you wish to measure at. To adjust the same capillary at differenttemperatures you can store it by different names in the capillary configurationslist.When finished, Lovis 2000 M/ME displays the ball runtimes at the adjustedangle(s) and the maximal variation coefficient that occurred during theadjustment. If one or more angles are invalid, the respective angles are notstored. The instrument displays a message.5. Tap to make sure the adjustment was correct.During a verification Lovis 2000 M/ME performs the full adjustment procedure fora second time.6. Prepare a vial with the same reference standard you have used for theadjustment. Follow the instructions on the display.TIPAfter a verification the runtime deviations to the values of the preceding originaladjustment are displayed for each adjusted angle. Typically, these values arehigher than the maximal variation coefficient of the original adjustment.C72IB002EN-E 321

25 Adjusting Lovis 2000 ME with Xsample SECTION II : Flow-Through Systems• For verification results within the specifications Lovis 2000 M/ME promptsyou to tap . stores the data of the verification asadjustment data of this capillary configuration.• If the maximal deviation exceeds the allowed deviation, Lovis 2000 M/ME prompts you to tap .• Fill the capillary again (using the same ball) and repeat the verification.• If still exceeding the allowed deviation after the second , clean andbrush the capillary (see Chapter 11.2), refill it and start a new adjustment.7. To print or export an adjustment report, tap .322 C72IB002EN-E

SECTION II : Flow-Through Systems26 Customer Maintenance Assemblies26 Customer Maintenance AssembliesTo keep your measuring assembly working smoothly and reliably, performregularly the following checks and maintenance work. The steps depend on theconfiguration of your measuring assembly.What to do:Visual inspection of hoses and hoseconnections.Tighten all hose connections.Leak test of all connected hoses.Special cleaning of the capillary.Visual inspection of capillaries andadapters.Xsample 352/452/52:Visual inspection of Xsample valve unitand piston pump.Xsample 122/22:Check the pump hose for wear ordamage.Xsample 122/22:Visual inspection of the peristaltic pumpfor contamination caused by a damagedpump hose.Regularly perform all maintenance stepsaccording to the schedule given for yourXsample model, if any.Regularly perform all maintenance stepsaccording to the schedule given forDMA M / DSA M, if any.Regularly perform all maintenance stepsaccording to the schedule given for allother modules used with your assembly, ifany.When:• Daily; before starting measurements.• After changing the capillary.• Monthly.• After changing the capillary.• Monthly.• After hoses were dis- andreconnected.• If leakage is suspected.See Chapter 26.2.• Once a year.• If cleaning outside the capillaryblock does not give satisfactoryresults.See Chapter 12.1.If residues inside the capillary aresuspected that cannot be removed bystandard flow-through cleaning. Cleanthe capillary manually. See Chapter 11.2.If required, perform a special cleaning. See Chapter 12.1.Daily; before starting measurements.Daily; before starting measurements.Daily; before starting measurements.Consult the instruction manual of yourXsample model.Consult the instruction manual of yourDMA M / DSA M model.Consult the instruction manual of therespective module (Alcolyzer ME,HazeQC ME, pH ME, Abbemat).C72IB002EN-E 323

26 Customer Maintenance Assemblies SECTION II : Flow-Through SystemsWhat to do:Replacing the O-rings of the capillaryadaptersWhen:• If the capillary adapters are leakingor do no longer hold tightly on thecapillary.• If changing to Kalrez ® O-rings forbetter chemical resistance.(See Chapter 12.5).26.1 Inspection of Hoses and Hose ConnectorsTo meet safety requirements and for proper function of the system, a regularinspection is required.1. Check all hoses and hose connectors of the measuring assembly at leastmonthly if they are connected properly. If required, tighten the connectors.2. Check the color of the FEP and PTFE hoses, especially of the hose usedwith the liquid sensors of Xsample 352/452. If this hose becomes very darkor turbid, the function of the optical sensors can be influenced. If required,replace this hose.3. Check all hoses for mechanical damage (e.g. kinks). Replace damagedhoses immediately.4. Xsample 122/22: Check the pump hose for damage and for proper fitting inthe peristaltic pump. A hose, which is not placed correctly, will wear earlier.26.2 Leak Test of all Connected HosesGenerally, this leak test works by applying air pressure to the complete systemwhile simultaneously closing the waste hose. This way air pressure builds up.Leakage can be detected by a hissing noise from the leaky part.26.2.1 Measuring Assemblies with Xsample 352/452Check the leak tightness of these hoses by performing the following easyprocedure before starting a magazine (Xsample 452) or after the respectivenumber of measurements (Xsample 352).1. Squeeze the waste hose manually so that it is leak tight.2. Tap to switch on the air pump (or, if used, the compressed air).The air flows via the valve block through the sensor hose, the hoses connectedto the Lovis capillary block, the Lovis capillary, the DMA M / DSA M measuringcell (if applicable), the needle hose and the needle into the waste hose.324 C72IB002EN-E

SECTION II : Flow-Through Systems26 Customer Maintenance Assemblies3. Listen to the noise of the air pump or the compressed air. Make sure that thenoise is not drowned out by ambient noise.• The noise disappears: the hoses are leak tight.• The noise does not disappear, you may hear also a hissing noise. Try tolocate the leakage.4. Tap to switch off the air pump (or compressed air).Reasons for leakiness• A hose connector may be loose - check and tighten all hose connectors andrepeat this check.• One of the hoses is damaged - check and replace the damaged hose andrepeat this check.• The O-rings of the filling adapters for the Lovis capillary may be damaged.Disassemble and check the parts. If required, replace the O-rings accordingto Chapter 12.5.• The vial cap seal or the PTFE insert in the Xsample waste block is damaged.Find solutions in the Xsample instruction manual.• The injection adapters of the DMA M / DSA M are loose or damaged (ifapplicable). Find solutions in the instruction manual of your DMA M/DSA M.26.2.2 Measuring Assemblies with Xsample 52Check the leak tightness of these hoses by performing the following easyprocedure after approx. 50 measurements.1. Tap to switch on the air pump (or, if used, the compressed air).2. For a soft, flexible sample filling hose (e.g. Silicone): Squeeze the hosemanually so that it is leak tight.For hard sample filling hoses: Attach a piece of silicone hose 2x4 onto theopen end of the sample filling hose. Squeeze the silicone hose manually sothat it is leak tight.The air flows via the valve block and the hose through the Lovis capillary, theDMA M / DSA M measuring cell (if applicable) to the sample filling hose.3. Listen to the noise of the air pump or the compressed air. Make sure that thenoise is not drowned out by ambient noise.• The noise disappears: the hoses are leak tight.• The noise does not disappear, you may hear also a hissing noise. Try tolocate the leakage.C72IB002EN-E 325

26 Customer Maintenance Assemblies SECTION II : Flow-Through Systems4. Tap to switch off the air pump (or compressed air). Remove the piece of silicone hose from the filling hose (if applicable).Reasons for leakiness• A hose connector may be loose - check and tighten all hose connectors andrepeat this check.• One of the hoses is damaged - check and replace the damaged hose andrepeat this check.• The O-rings of the filling adapters for the Lovis capillary may be damaged.Disassemble and check the parts. If required, replace the O-rings accordingto Chapter 12.5.• The injection adapters of the DMA M/DSA M are loose or damaged (ifapplicable). Find solutions in the instruction manual of your DMA M/DSA M.26.2.3 Measuring Assemblies with Xsample 122/22Check the leak tightness of these hoses by performing the following easyprocedure before starting a magazine (Xsample 122) or after the respectivenumber of measurements (Xsample 22).Depending on the type of waste hose, you need to close this hose in a differentway.1. Tap to switch on the air pump. Xsample 122 only: The needle movesupwards into service position:• Xsample 122 only: remove the Xsample safety cover,• connect the air hose to the needle tip,• turn the pump lever into horizontal position and lift the cartridge to releasethe pump hose.2. For soft, flexible hoses (e.g. Silicone): Squeeze the waste hose manually sothat it is leak tight. For FEP or other hard hoses: screw an adapter UNF/UNF onto the free endof the waste hose. Screw an adapter Luer 1/4" UNF (Lovis accessories) intothis adapter. Close the adapter Luer 1/4" UNF tightly with a Luer plug (Lovisaccessories).The air flows via the needle through the hoses into the DMA M / DSA Mmeasuring cell (if applicable) and then through the Lovis capillary into the wastehose.3. Listen to the noise of the air pump or the compressed air. Make sure that thenoise is not drowned out by ambient noise.• The noise disappears: the hoses are leak tight.326 C72IB002EN-E

SECTION II : Flow-Through Systems26 Customer Maintenance Assemblies• The noise does not disappear, you may hear also a hissing noise. Try tolocate the leakage.4. When ready, remove the air hose from the needle.5. Tap to switch off the air pump (or compressed air).• Re-attach the cartridge an turn back the pump lever into vertical position.• Xsample 122 only: Re-attach the safety cover.Xsample 122 only: The needle moves back into initial position.6. If initially attached, remove the Luer plug, the adapter Luer 1/4" UNF and theadapter UNF/UNF from the waste hose. Then re-connect the waste hose toyour waste container.Reasons for leakiness• A hose connector may be loose - check and tighten all hose connectors andrepeat this check.• One of the hoses is damaged - check and replace the damaged hose andrepeat this check. A worn pump hose may be the reason for leakage.• The O-rings of the filling adapters for the Lovis capillary may be damaged.Disassemble and check the parts. If required, replace the O-rings accordingto Chapter 12.5.• The injection adapters of the DMA M / DSA M are loose or damaged (ifapplicable). Find solutions in the instruction manual of your DMA M / DSA M.C72IB002EN-E 327

Appendix A: Technical DataAppendix A:Technical DataA.1 Measuring PerformanceLovis 2000 MLovis 2000 ME &DMA MMeasuring range• Dynamic viscosity 0.3 mPa.s to 10 000 mPa.s• Inclination 15° to 80° inclination in 1° stepsLovis 2000 ME &DSA 5000 M• Shear rateapproximately 0.5 s -1 to 1000 s -1 influenced by capillarysize and inclination• Density – 0 g/cm 3 to 3 g/cm 3• Sound velocity – –1000 m/s to2000 m/sTemperature ranges• Viscosity measurement +5 °C to 100 °C (41 °C to 212 °F)• Density measurement –0 °C to 95 °C(32 °F to 203 °F)• Sound velocity measurement – –Precision of Lovis 2000 M/ME a• Temperature repeatability s.d. 0.005 °C (0.01 °F)• Temperature accuracy 0.02 °C (0.04 °F)• Inclination repeatability s.d. 0.02°• Inclination accuracy 0.1°• Measuring time resolution 0.001 s• Measuring time accuracy 0.05 %• Viscosity repeatability s.d. up to 0.1 % b0 °C to 70 °C(32 °F to 158 °F)0 °C to 70 °C(32 to 158 °F)• Viscosity accuracy up to 0.5 % b, cFurther specifications• Test duration minimal 30 s, typical 3 min.minimal 60 s,typical 4 min.• Minimum sample volume withoutusing a sample changer d- Standard capillaries 0.4 mL to 0.8 mL 1.5 mL to 3 mL 3.5 mL to 5 mL- Short capillaries 0.1 mL to0.25 mL– –a. For precision specifications of density and sound velocity measurement refer to the instruction manual of therespective DMA M/DSA M model.b. Depends on sample type and measuring settings, without changing the ballc. For the range in which the adjustment constant was determined, not including uncertainty of reference standardsd. Minimum sample volume for measuring assemblies see Appendix A.4328 C72IB002EN-E

Appendix A: Technical DataA.2 Lovis 2000 M/ME Capillary Measuring RangesCapillaryLovis 2000 M/ME capillary measuring ranges (dynamic viscosity)runtime extended range a core range blong distance 10 s 1700 s 10 s 1700 sshort distance 2.5 s 425 s 2.5 s 425 s1.59 mmsteel ball 0.3 mPa.s 90 mPa.s 1 mPa.s 20 mPa.sgold ball 0.2 mPa.s 65 mPa.s 0.7 mPa.s 15 mPa.s1.8 mm steel/gold ball 2.5 mPa.s 1700 mPa.s 13 mPa.s 300 mPa.s2.5 mm steel/gold ball 12 mPa.s 10 000 mPa.s 70 mPa.s 1700 mPa.sa. Allows measurements in an extended viscosity range, but not at all angles. Very low viscous liquids can be measuredat flat angles down to 15°, very high viscous samples can be measured at steep angles of 75° or 80°.b. Allows to measure the viscosity at all angles from 15° to 80° at measuring temperature with one capillary. Alladjustments should be performed using reference liquids within the core range of the used capillary.TIPFor short capillaries only the short distance is applicable. The viscosity measuringrange is the same as for standard capillaries with the same diameter.A.3 Recommended Viscosity Standard LiquidsCapillaryRecommendedTemp. RangeStd.LiquidFw/BwDeviationRecommended Limit [%]VariationCoeff.Repeatability aDeviation b1.59 mm 20 °C to 40 °Cwaterbi-dist.0.5 % 0.1 % 0.15 % 0.75 %1.59 mm 20 °C to 80 °C S3 oil 1 % 0.15 % 0.25 % 1 %1.59 mm 40 °C to 100 °C N7.5 oil 1 % 0.15 % 0.25 % 1 %1.8 mm 20 °C to 100 °C N26 oil 1 % 0.15 % 0.25 % 1 %2.5 mm 20 °C to 60 °C N100 oil 1 % 0.2 % 0.75 % 1 %2.5 mm 40 °C to 100 °C N415 oil 1 % 0.2 % 0.75 % 1 %a. Standard deviation [%]b. For the range in which the adjustment constant was determined, including uncertainty of ref. standard (approx.0.25%; depending on the supplier and the viscosity range).TIPIf using bi-distilled water as standard liquid, take care to degas it first.Put your sample in a covered vessel into an ultrasonic bath for approximately10 minutes. Choose a temperature higher than for the Level or CapillaryAdjustment. Fill the capillary immediately after degassing.Alternatively, boil the water for several minutes to remove dissolved air. Fill aclean glass flask full with the boiled liquid and cover it. Wait until the liquid hascooled down to the approximate adjustment temperature.C72IB002EN-E 329

Appendix A: Technical DataA.4 Minimum Required Sample Volume AssembliesManual flow-through filling by syringe, single determinationMeasuring assemblyhosesmin. volume[mL]Lovis + AbbematDMA M + Lovis + Abbematstandard 5.5low volume a2.6standard 6.5low volume a 3.6DSA M + Lovis + Abbematstandard 8.5low volume a 5.6DMA M + Lovis + pH standard 13DSA M + Lovis + pH standard 15a. Optional. See Chapter 16.2.4.Filling with sample changer, single determinationMeasuring assemblyhoses,vialsmin. volume a[mL]standard, 12 mL 7.5Lovis + Xsample 352/452Lovis + DMA M + Xsample 352/452low volume b , 12 mL 4.5low volume b ,low volume 6 mL c 2.5standard, 12 mL 9low volume b , 12 mL 5.5low volume b ,low volume 6 mL c 3.5Lovis + Xsample 52 12 mL 5 dLovis + DMA M + Xsample 52 12 mL 7 dLovis + Xsample 122Lovis + DMA M + Xsample 122Lovis + DSA M + Xsample 122Lovis + Xsample 22Lovis + DMA M + Xsample 2212 mL 850 mL 2012 mL 1050 mL 2012 mL 1250 mL 2012 mL 850 mL 2012 mL 1050 mL 20330 C72IB002EN-E

Appendix A: Technical DataLovis + DMA M + Alcolyzer ME +HazeQC ME + pH ME +Xsample 122 (Analyzing system forbeer and wort)Lovis + DMA M + HazeQC ME +Abbemat + pH ME + Xsample 122Lovis + Xsample 352/452 +AbbematLovis + DMA M + Xsample 352/452+ Abbemat50 mL 4050 mL 40low volume e , 12 mL 5low volume e , 6 mL c 4low volume e , 12 mL 7low volume e , 6 mL c 6a. Especially with Xsample 22/Xsample 122 the sample volume may be higher to avoid samplecarryover. For Xsample 22 additionally the shape of the sample vessel has an influence on thesample volume.b. Optional. See Chapter 16.2.4.c. Suitable only for Xsample 452 with optional low volume magazine. For Xsample 352 only thestandard vials 12 mL can be used.d. Depending further on the shape of the sample vessel.e. This measuring assembly can only be used with low volume hoses.C72IB002EN-E 331

Appendix A: Technical DataA.5 General Technical DataLovis 2000 MLovis 2000 ME &DMA 4100/4500/5000 Mor DSA 5000 MLovis 2000 ME inModule HousingDimensions (L x W x H) 482 x 420 x 231 mm (19.0 x 16.5 x 9. 1 in)400 x 315 x 231 mm(15.7x 12.4 x 9.1 in)Net weight 17.0 kg (37.4 lbs) 27.3 kg (60 lbs) 9.3 kg (20.5 lbs)Voltage, Frequency AC 100 V to 240 V; 50 Hz to 60 Hz DC 24 VPower 190 VA max. 40 W max.Power socketFusesAccording to IEC/EN 60320-1/C14,protection class I2 glass tube fuses 5x20 mm; IEC60127-2;AC 250 V; 5 A slow ceramics--Power supply bymaster instrumentvia CAN BusSMD fuse3.15 AFPressure range a • 1 MPa (10 bar): up to 50 °C (122 °F)• 500 kPa (5 bar): 50 to 100 °C (122 °F to 212 °F)Housing materialTop & side coverAluminum, coated with a poly acrylate resin varnishBackAluminum and stainless steelFrontStyrene/Butadiene, coated with a poly acrylate resin varnishEnvironmental conditions(EN 61010)Indoor use onlyAmbient temperature15 °C to 35 °C (59 °F to 95 °F)Air humidity10 % to 90 % relative humidity, non-condensingPollution degree 2Radiated emissionsClass A. This equipment can generate radiated disturbances inresidential environment.Overvoltage categoryIIAirborne noise emitted< 60 dB/AAltitudemax. 3000 m (9800 ft)Touch screen 6.4 in TFT, 640 x 480 px --Memory 1000 measuring values --InterfacesRS-232 printer settings• 4 x USB (2.0 full speed)• 1 x Ethernet (100 Mbit)• 1 x CAN Bus• 2 x S-Bus b• 1 x RS-232• 1 x VGABaud rate: 9600; Parity: None; Stop bit: 1;Data bits: 8a. Applicable only for flow-through systems with FEP or PTFE hoses with UNF threadsb. Only for Lovis 2000 ME in combination with DMA M/DSA M models• 2 x CAN Bus--332 C72IB002EN-E

Appendix A: Technical DataWARNINGSerious injuries are possible through high voltage if the following hints are notadhered to.• Only connect devices to the interfaces that comply with PELV (protectiveextra-low voltage) according to EN 61140 or with SELV (safety extra-lowvoltage) according to EN 60950.NOTICEConnect only Anton Paar equipment to the CAN interface and adhere to thefollowing limits. The CAN interface power supply is limited to 72 W.• For Lovis 2000 M models with serial no.s lower or equal 81002856, theconsumption of modules in the base unit’s slot has to be subtracted:- If the master instrument contains an Xsample module, additionalequipment must not exceed a maximum power consumption of 40 W.- If the master instrument contains a Lovis 2000 ME module, additionalequipment must not exceed a maximum power consumption of 32 W.• From Lovis 2000 M model with serial no. 81002863 on, equipment with72 W can be connected to the external CAN interface independent of thebuilt-in module.If a module (or several) with higher power requirement shall be connected, aseparate CAN power supply (Mat. No. 100655) must be provided.C72IB002EN-E 333

Appendix B: Wetted Parts and Chemical CompatibilityAppendix B:Wetted Parts and ChemicalCompatibilityThe following materials of Lovis 2000 M/ME and its accessories are in contactwith samples and cleaning liquids. If using Lovis 2000 ME combined with aDMA M / DSA M model and with Xsample sample changers, refer to therespective instruction manual for the materials of the density meter/sound velocityanalyzer or sample changer.B.1 Wetted PartsTIPFor detailed information to changes of Lovis 2000 M/ME wetted parts consult theProduct Description List supplied on the USB flash drive with your Lovis.Lovis 2000 M/ME and accessories required for operationMaterialBorosilicate glass 3.3PVDF (Polyvinylidene fluoride)PCTFE (Poly Chloro Tri FluoroEthylene)PTFE (= Poly tetrafluoroethylene)FPM Viton ® Extreme (ETP600-S)Kalrez ® Spectrum 6375Part• Measuring capillary• Glass syringe (250 µL, 10 µL)• Waste bottle 1 L - optional item• Plug (for closing the capillary atthe end)Up to 11/2012:• Filling and Cleaning Adapter LuerMale• Filling Adapter UNF Front Side• Inner part of Filling Adapter UNFBack Side Complete (part that sitson the capillary)From 11/2012 on:• Filling Adapter Luer Female• Hollow plug (for closing thecapillary at the end - for use withshort capillaries, optional for thestandard capillaries if betterchemical resistance is required)• Filling and Cleaning Adapter LuerMale• Filling Adapter UNF• Plunger tip and Luer cone of glasssyringeO-rings of filling adaptersO-rings of filling adapters - optional itemsETFEtetrafluoroethylene)(Ethylene-Luer plug with and w/o bore (for closingthe capillary at the top)334 C72IB002EN-E

Appendix B: Wetted Parts and Chemical CompatibilityPEEK (black) (Polyether etherketone)Stainless steel 1.4125Stainless steel 1.4301 (SS304)Stainless steelGold (4 µm)PA 6.6 (Polyamide 6.6; Nylon),stainless steelGoat hair, stainless steelPP (Polypropylene)PE (Polyethylene)HDPE (High density polyethylene)Stainless steelChrome-plated brassFilling Support (for flow-through filling withsyringe)Standard ballsHollow needles (Luer lock) for fillingand cleaning of short capillariesLuer cone of glass syringe (outsideonly)Gold coated steel balls aBrush (for cleaning)Brush (for cleaning) - optional itemTransparent part of Luer syringeGreen plunger of Luer syringeWaste bottle 0.5 LLock device of 10 mL glass syringeMetal parts of 250 µL glass syringea. Steel balls 1.4125 with a 3 µm nickel undercoating and a 4 µm gold top coatingC72IB002EN-E 335

Appendix B: Wetted Parts and Chemical CompatibilityAccessories required for use with DMA M/DSA MMaterialFEP (Fluorinated ethylenepropylene)PTFE (Poly tetrafluoroethylene)Tefzel = modified ETFE (Ethylenetetrafluoroethylene)PartHoses for connection to DMA M/DSA M• Injection adapter UNF 1/4" DSA• Injection adapter UNF 1/4" DMA• Screw adapters of FEP hoses• Female Luer to 1/4"-28 UNF MaleAdapterFurther accessories required for use with measuring assembliesXsample models with and w/o DMA M/DSA MMaterialFEPNorprene ® ChemicalNorprene ® A-60-GTygon ® 2375SiliconeViton ® FKM 75PCTFETefzel (ETFE)Stainless steel 1.4571Part• Hose• Liner (inside) of Norprene ®Chemical hoseChemical resistant pump hose forXsample 122/22 (Chemicalsgenerally)Chemical resistant pump hose forXsample 122/22 (Petro)HoseHoseHose• Adapter UNF/UNF• Adapter UNF/Hose (Female/Male)Adapter UNF/screwed tube• Tube d=2.1 D=2.4 L=20• Tube of adapter UNF/screwedtubeAll these items are also described in the Lovis 2000 M/ME Product DescriptionList (on USB flash drive).336 C72IB002EN-E

Appendix B: Wetted Parts and Chemical CompatibilityB.2 Parts Possibly in Contact with Sample LiquidThis chapter lists parts of Lovis 2000 M/ME which may under certaincircumstances (leakage, overfilling of the capillary) also come in contact withsample. It is to be expected that these parts will become dirty if the instrument isconstantly in operation and/or is used in harsh environments. This lists also helpsto select a suitable cleaning liquid.MaterialPOM-C(Polyoxymethylene)(black)Part• Screw Cap Front Side Closed• Screw Cap Back Side Closed• Screw Cap Front Side Open• Outer part (screw cap) of FillingAdapter UNF Back Side Complete• Screw Cap Back Side Open (from 11/2012)• Funnel for 1.5 mm balls• Cover of Lovis capillary blockPA 12 (Vestamid ® ; Polyamide12)PVDF (Polyvinylidene fluoride)PEEK (Polyether ether ketone)PEEK (black; Polyether etherketone)CopperStainless steel (1.4310)Plastic tweezers for ballsTip of ball dispenser• Fixing ring inside Filling Adapter UNFBack Side Complete (up to 11/2012)• Inserts on end of Lovis capillary blockFilling Support UNF (for flow-throughfilling with syringe and for filling hoses incombination with some sample changermodels; from 11/2012)Inside of the Lovis capillary blockSpring of Filling Adapter UNF Back SideComplete (up to 11/2012)C72IB002EN-E 337

Appendix B: Wetted Parts and Chemical CompatibilityB.3 Improving the Chemical ResistanceIf your application requires a better chemical resistance, you can replace thefollowing parts by parts with improved chemical resistance:O-rings and ballsThe Lovis Chemical Resistance Upgrade Set, Mat. No. 101899, contains itemswhich offer the optimum chemical resistance against aggressive substances thatcan be measured with Lovis 2000 M/ME. Comes with:• 1 set of balls 1.5 mm 4 µm gold (300 pcs.), Mat. No. 20659• 4 pcs. O-ring 5x1.5 Kalrez® Spectrum 6375, Mat. No. 101519• 1 sticker "Made with Kalrez®", Mat. No. 101927The O-ring 5x1.5 Kalrez® Spectrum 6375 is also available separately per piece,Mat. No. 101519. When ordering the O-rings separately, one sticker "Made withKalrez®" is included in the delivery.Replace the Viton® Extreme O-rings of the capillary filling adapters by theKalrez® ones according to Chapter 12.5.Attach the sticker "Made with Kalrez®" visible on the instrument’s casing to avoidthat the Kalrez® O-rings are replaced by less resistant O-rings during service ormaintenance.TIPWhen using the short capillaries for aggressive substances it is not required tochange the O-rings as they are not in contact with the sample.Capillary filling adaptersAll capillary filling adapters and the filling and cleaning adapter delivered from 11/2012 are made of PCTFE due to a better chemical compatibility. If you havealready the previous version made of PVDF, you can replace them by the newPCTFE adapters. Consult the Product Description List on the USB flash drivesupplied with your Lovis.Capillary plugsThe standard capillary plugs are made of PVDF, they can be replaced bycapillary plugs made of PCTFE originally designed for the short capillaries:• Capillary plug hollow 1.59 (20 pcs.; PCTFE), Mat. No. 108489• Capillary plug hollow 1.8 (20 pcs.; PCTFE), Mat. No. 108490• Capillary plug hollow 2.5 (20 pcs.; PCTFE), Mat. No. 108491Consult the Product Description List on the USB flash drive supplied with yourLovis.338 C72IB002EN-E

Appendix B: Wetted Parts and Chemical CompatibilityManual flow-through fillingFor filling chemically aggressive substances that attack the disposable plasticsyringes, a precision glass/PTFE syringe 10 mL Luer Lock, Mat. No. 109045, isavailable (syringe barrel: Borosilicate glass 3.3; plunger tip and Luer cone: PTFE;lock device: stainless steel).It requires the filling support with UNF thread and an adapter Luer/UNF (Mat. No.64792, supplied with the standard delivery) screwed into the support.Note: If your instrument is not equipped with the filling support UNF, contact yourlocal Anton Paar representative.To collect chemically aggressive sample and solvent liquids, a waste bottle madeof Borosilicate glass 3.3 is available.Waste bottle glass 1 L, Mat. No. 101655, with protective netting and insert withthree 1/4"-28 UNF threads to connect hoses.Refer to Chapter 17.4.2. Hoses for flow-through filling with Xsample 122/22These kits are designed to make the Xsample 122/22 sample changer fit for usewith a wide range of chemicals ("Chemical") or for use with petrochemicalsamples ("Petro").• Retrofit kit for Xsample 122 Chemical, Mat. No. 96878• Retrofit kit for Xsample 122 Petro, Mat. No. 96880• Retrofit kit for Xsample 22 Chemical, Mat. No. 96879• Retrofit kit for Xsample 22 Petro, Mat. No. 96881Consult the Product Description List on the USB flash drive supplied with yourLovis. If questions are left, contact your local Anton Paar representative.For retrofitting your measuring assembly with these hoses, refer to the instructionmanual of your Xsample model and to the following sections in this manual:For Lovis + Xsample 122: Chapter 18.3.3For Lovis + Xsample 22: Chapter 18.4.3For Lovis + DMA M/DSA M + Xsample 122: Chapter 19.3.3For Lovis + DMA M/DSA M + Xsample 22: Chapter 19.4.3C72IB002EN-E 339

Appendix B: Wetted Parts and Chemical CompatibilityB.4 Overview Chemical CompatibilityWetted partsA ... ExcellentB ... Go o d, mino r effectC ... Fair, needs further testsD ... UnsuitableBorosilicate glass 3.3Stainless steel A4; 1.4125GoldPVDF (Polyvinylidenefluoride)PCTFE (Poly Chloro TriFluoro Ethylene)Viton® 981 Extreme (ETP600S)Water A A A A A A A A A AAceonitrile A A A A B A ACresols (o-, m-, p- methylphenols) A A A A A B/C AEthanol A A A A A A A A A AMethanol A A A B A A A A C AAcetone A A A C A B A A C ADichloromethane A B A B B A B C ADichlorophenole (2-Chloroph., OCP) A A A A A ASulfuric acid A C-D A A A A A B A AFormic acid A B A A A C A A A APhenol A B A A A A A A C ATetrachloroethane A B A A A A B C ADichloroacetic acid A B A A C A A AAmines A A A A/B B A B/C AChloroform A D A A A A A D D ADecahydronaphtalene (Decalin) A A A A A A C/D AToluene A A A B B A A B D ACyclohexanone A C A C A C A A D ATetrahydrofuran (THF) A A A B/C B C B B C AMethyl ethyl ketone (MEK) A B A D A B A A C ASolvent naphtha A B A A D A A A C An-Hexane A A A A A A A A B AGlycol A B A A A A A A C APropanole (1-Propanole, n-Propanole) A A A A A A A A A A2-Propanole (Isopropyl alcohol, IPA) A A A A A A A B A AETFE (Tefzel)PE (Polyethylene)PP (Polypropylene)Kalrez 6375 *Personal notes:* optional O-rings for the filling adapters340 C72IB002EN-E

Appendix B: Wetted Parts and Chemical CompatibilityAccessories for use with DMAM/DSA M and XsampleParts possibly in contact w ithsample liquidFEP (Fluorinated Ethylene Propylene)PTFE (Teflon)A ... ExcellentB ... Go o d, mino r effectC ... Fair, needs further testsD ... UnsuitableWater A A A A A A A A A A A BAcetonitrile A A A A D A A ACresols A A A A C-D C-D A-B AEthanol A A B A C A A D A A A AMethanol A A A A A A A D C A A BAcetone A A D A D D A A A A A ADichloromethane A A D B D B/C C ADichlorophenole (2-Chloroph., OCP) A A A BSulfuric acid A A D A D A D D D D DFormic acid A A D A B D D C D B/C A/B CPhenol A A D A B/C A D D C A DTetrachloroethane A A D A B C ADichloroacetic acid A A D A DAmines A A A/BDecahydronaphtalene (Decalin) A A A A A A AChloroform A A D A B A/B A D D D A AToluene A A D B D A B A A A ACyclohexanone A A D A D D A A A BTetrahydrofuran (THF) B B D B D A AMethyl ethyl ketone (MEK) A A D A D D B B B/C ASolvent Naphtha A A C/D A C A A A An-Hexane A A B A B A A A A A A AGlycol A A A A A C APropanole (1-Propanole, n-Propanole) A A A A A2-Propanole (Isopropyl alcohol, IPA) A A C A A A C A BSiliconePCTFE (Poly Chloro Tri Fluoro Ethylene)Tygon® 2375Viton® FKM 75POM-C (Polyoxymethylene copolymer)Vestamid® (Polyamide 12; PA 12)Nylon (Polyamide 6.6, PA 6.6)Stainless steel 1.4310PEEK (Polyether ether ketone)CopperPersonal notes:C72IB002EN-E 341

Appendix C: Menu TreeAppendix C:Menu TreeThe menu tree shows the default configuration for Lovis 2000 M/ME. Dependingon the master instrument or the module a Lovis 2000 ME is combined with,additional menu items are available. Refer to the instruction manual of therespective instrument.AdministratorAdministrator, ManagerAdministrator, Manager, OperatorThe colors show which parts of the menuare accessible for users withadministrator, manager or operator rights.Tap to access:Checks/AdjustmentsChecks Water Check (default) Select and perform a check measurement (default oruser-defined custom check if any)Other Adjustments Lovis Module Level AdjustmentStandard AdjustmentExtended AdjustmentSingle PointAdjustmentTemperatureAdjustmentTemperatureAdjustment withMKT50Reset to Temp.Factory Adjustment(for capillaries; angles20° to 70°)(for capillaries, angles15° to 80°)(for capillaries, at oneuser-defined angle)Data Memory Measured Data Select (filter), print or export measured dataStatisticSelect (filter) stored measurements and view, print or export their statistic data(mean value, std. dev.)Delete MeasuredDataSelect (filter) and delete measured dataAudit TrailView, print or export the audit trail (protocol of log on/log off, failed log on,password changes, electronic signature)Check DataView, print or export data of check measurementsDelete Audit TrailAdjustment Data Lovis Module Capillary AdjustmentPrint or ExportAdjustment DataTemperatureAdjustmentLevel AdjustmentDistance AdjustmentView, print or exportadjustment dataSelect all adjustment data of Lovis 2000 M/ME or ofany other connected instrument, print or export themLovis Utilities Initialization Initializes Lovis 2000 ME. Moves the Lovis capillary block into the default position(-45° for manually filled system, +20° for flow-through system).Fan ServiceStart/Stop the Capillary block fan342 C72IB002EN-E

Appendix C: Menu TreeMethods Method Settings a Lovis standard Activate Modules /(Current Method) b Constants cMeasurement Mode(S)Repeated ModeSettingsLovis ModuleDisplay LayoutResult OutputLimitsFormula ParametersQuick SettingsManagementa. Other methods than the factory predefined ones shown here can be created by the user.b. This is the factory default setting. All method settings are available for each method.c. Available only if a "Constant" user function was created and/or if a module (e.g. Xsample) is connectedd. Available only in combination with DMA M/DSA M.e. Available only for instrument firmware version 2.32.Select a meas. modeand set its parametersSet parameters for Lovisrepeat deviation (max.no. of determinationsand max. repeat value)Temperature Settings(Set the temperatureand temperatureequilibration time)Measuring Settings(Meas. cycles, angle,distance, variationcoefficient)Advanced Settings(max. no. of meas.cycles, thresholdvalues for automaticangle and measuringdistance setting,measuring timeout forlong distance)Density Settings dUse density meas.values or manualdensity inputBeer/Wort Setting(special setting forbrewing application)Polymer Settings e(special settings forpolymer applications)Molar Mass eCalculation Settings(special settings forpolymer applications)Set no./ size of outputfields on the screenSelect the outputquantities, their units,their order and thesignificant digitsSelect a quantity andassign limit valuesEnter density value(s)Sort, add or delete itemsin the Quick Settings listC72IB002EN-E 343

Appendix C: Menu TreeMethods Method Settings a Lovis detailedMethod ManagementMethod VisibilityLovis / DMALovis Polymer (SingleConc.) bLovis Polymer (Multi Conc.) bAvailable only in combination with DMA M/DSA MMethod for polymer applicationsMethod for polymer applicationsCopy/Rename/Delete methods. Delete/Rename not allowed for current method.Set which methods can be selected for measurement (button ) or forrenaming/deleting (in Method Management). The current method cannot bemade invisible. Arrange in which order methods shall be displayeda. Other methods than the factory predefined ones can be created by the user. The submenu items explained for method"Lovis standard" are also available for all other methodsb. Available only for instrument firmware version 2.32.SetupMeasuring SystemSettingsLovis ModuleSample ListAir PumpTemperatureExtrapolationSettings aa. Available only for instrument firmware version 2.32.CapillaryAdministrationCapillary DetectionCapillary Filling ModePolymer SolventsAdministration aSample List / SampleSettingsUser-defined DataFieldsSample List WarningsMandatory DataFieldsAdd, delete, editcapillariesEnable/disable theautomatic detectionManually or flow-throughStore measurement dataof polymer solvents forlater useEnable/Disable samplelist, set an automaticsample name withprefix/postfix, PreviewEnable/Disable fields,Name fieldsEnable/Disable: Stopprocessing if check failsWarning if list contains:- sample without name, - methods with differentmeas. temperaturesSet which data fieldsrequire mandatory inputEnable/disable automatic stop, set run timeSet temperature values and units (°C / °F) to whichmeasuring values can be extrapolated.344 C72IB002EN-E

Appendix C: Menu TreeSetup Control Panel Date and TimeRegional SettingsInput UnitsPrinter ManagementNetworkInstrument Name andLocationScreen SaverFeedback Beeps /Input Beep aCalibrate TouchScreenCalibrate ExternalTouch ScreenKeyboard and BarCode SettingsNetwork ShareAdministrationSMTP ServerSettingsSelect language, data format and keyboard layoutSelect between °C / °F / K for temperature inputSelect, edit or add printers; PDF File Printer = defaultEnter IP address, subnet mask, default gateway,primary and secondary DNSEnable/disable and set the activation timeSelect when a beep should be emitted(measurement done or when touching a screen itemor key)Enable/disable bar code readerSet Name, Path, User Name and Password forstoring data to a network instead of to USB flashdriveSet Host, Port, User Name, Password and addressfor Email notification; test the settingsDisplay Brightness Set the display backlight brightness (20 % to 100 %)System Security Security Level Low or 21 CFR Part 11, view details for each settingAudit TrailEnable/disable function and reminder for backup file,set a number of daysElectronic Signature Enable/disable electronic signature and passwordLogon Settings Enable/disable and set time for:Auto-Logoff (1 to 60 min)Password expiry time (1 to 60 days)Increased Security Enable/disable; view detailsAdvanced Security b Disable USB flash drives Service logon only with customer passwordUser Management • Administrator: Add/edit/delete users, change passwords, activate user accounts and auto logon. Define E-mail settings• Manager: Change manager password• Operator: Change operator passwordPrintout Settings Automatic Printout Activate automatic printout, set printout forsubsamplesData BrowserSettingsa. The Menu item’s name depends on the instrument firmware version.b. Available only for instrument firmware version 2.32.Report Management Edit report format, set the default reportSelect LogosReport logo – select from listReport background – select from listImport LogosImport logos from external sourceName and Address Enter report heading - max. 50 characters per fieldAdd, sort, delete quantities to be viewed in the data browserC72IB002EN-E 345

Appendix C: Menu TreeSetup Expert Settings User FunctionsManagementDelete User FunctionCalculatorGroup CalculatorCheck ManagementData MemorySettingsCreate, edit, delete user functions, e.g. calculationsSelect functions and units to verify.Calculate instrument related quantities.Water Check; edit checks / create new checks (custom type) /delete custom type checksEnable/disable: automatically delete oldmeasurement dataServiceBackup InstrumentSettingsRestore InstrumentSettingsSave settings file (.abf) to USB flash drive – file name: max. 50 characters; selectdata to be stored: Global Settings, Method Settings, User Functions, Users,AdjustmentsRead settings file (.abf) from USB flash driveUpdate System Update Read file from USB flash drive (.aup file)Module Firmware Read file from USB flash drive (.afp file)UpdateInstall Language Read file from USB flash drive (.alp file)PackLoggingFor service purposes only (.alc file)ConfigurationSystem Information Shows all versions of hardware, module and instrument firmware and serialnumbersSave an instrument Logfile hereIQ/OQ ReportPrint or export an IQ/OQ ReportExport InstrumentDataExport all capillaryconfigurationsShow Lovis CountersLive Raw DataExport measured data, last adjustments and system information to USB flashdrive in form of an Excel workbookSave all capillary configurations and corresponding adjustment data to USB flashdriveView operating hours, no. of shake test cycles and no. of measurement cyclesLovisCalculationsView list of calculations of the currently availableoutput quantitiesDCB - Air Pump View status of air pump (0 or 1)DCB - Environment View heatsink temp. and temp. inside instrumentDCB - CANView current value at CAN interfaceConnector346 C72IB002EN-E

Appendix D: Lovis ParametersAppendix D:Lovis ParametersD.1 Output Data GroupsOutput data of the Lovis 2000 M/ME are divided into groups:• System – contains system relevant information.• Lovis – contains Lovis specific quantities such as dynamic viscosity.• Lovis Polymer - contains result parameters for testing polymer solutions(only available for instrument firmware version 2.32).• Depending on the measuring assembly further groups are available, e.g. forDensity, API Functions or Sampling Unit. Refer to the manuals of therespective instruments or modules.Parameters of data group "System"DataField1/2/3 – free data fields, can be user defined (see Appendix C/Setup or Instrument Firmware Manual)Date – current date (see Appendix C or Instrument Firmware Manual)Last Check Date – see Chapter 8.2Last Check Name – see Chapter 8.2Last Check Result – see Chapter 8.2Last Check User – see Chapter 8.2Master Condition – sum of all available conditions from measuringmodules within the system (valid / error state)Measurement Mode – see Chapter 7.2.3Method – see Chapter 7.2Parent Sample Id – sample ID of the parent sample, used withmeasurement modes that perform multiple measurements with the samesample.Sample Error State – see Chapter 10.3Sample List Number – number of the current sample list entry (seeInstrument Firmware Manual)Sample Name – sample name entered by the userSample Type – information on measurement mode, parent sample ID andsub measurement number.Serial Number – instrument serial numberSub Measurement Number – sub no. of a determination, used with meas.modes that perform multiple measurements with the same sampleTime – current time see Appendix C or Instrument Firmware ManualUnique Sample ID – internal unique sample ID that can not be resetUser – current user (see Instrument Firmware Manual)C72IB002EN-E 347

Appendix D: Lovis ParametersParameters of data group "Lovis"Lovis Angle – set measuring angleLovis Average Runtime – average runtime of one measurementLovis Beer/Wort Extract – specific value for beer measurement (seeChapter 7.2.5.5)Lovis Capillary Info – shows current angle of Lovis capillary block, nameand serial no. of selected capillary and the capillary status (inside/outside)Lovis Condition – see Chapter 10.3.1Lovis Corr. Viscosity 12% – specific value for beer measurement aLovis Corr. Viscosity 8.6 % – specific value for beer measurement aLovis Density – manually entered sample density valueLovis Dyn. ViscosityLovis Fw/Bw Deviation – runtime deviation between forth and back runLovis Kin. Viscosity – kinematic viscosityLovis Meas. Distance – used measuring distanceLovis Set Temperature – set measuring temperatureLovis Shear RateLovis Temperature – actual temperature inside the Lovis capillary blockLovis Variation Coefficient – see Chapter 7.2.5.2Lovis Viscosity (%SWE) – sea water equivalent; specific value for testingbrine solutionsa. The viscosity of wort is commonly converted to a specific extract content (8.6 % or12 %Plato) for a better comparability.As the viscosities are not proportional to a dilution but follow a hyperbolic function,the viscosities are converted applying a trigonometrical function developed byZuercher (MEBAK 3.1.4.4).Parameters of data group "Lovis Polymer"These parameters are calculated result values for polymer applications.Find the equations for some of these parameters in the Application Report onPolymer Solutions (Doc. No. C72IA023EN, supplied on the USB flash drive).Inherent ViscosityIntrinsic Viscosity (Billmeyer)Intrinsic Viscosity (Deb-Chatterjee)Intrinsic Viscosity (Huggins)Intrinsic Viscosity (Kraemer)Intrinsic Viscosity (Maron)Intrinsic Viscosity (Martin)Intrinsic Viscosity (Schulz-Blaschke)348 C72IB002EN-E

Appendix D: Lovis ParametersIntrinsic Viscosity (Solomon-Ciuta)K-ValueMolar Mass (Billmeyer)Molar Mass (Deb-Chatterjee)Molar Mass (Huggins)Molar Mass (Kraemer)Molar Mass (Maron)Molar Mass (Martin)Molar Mass (Schulz-Blaschke)Molar Mass (Solomon-Ciuta)Polymer Concentration (m/m)Polymer Concentration (m/v)R 2 (Zero Shear Relative Viscosity)Reduced ViscosityRelative ViscositySpecific ViscosityZero Conc. Relative ViscosityZero Shear Viscosity – This output parameter is not only applicable forpolymer applications, but also if performing a ZSS (Zero Shear Scan).See Chapter 7.2.3.D.2 Lovis Quick Settings ParametersThe quick settings parameters set by default are:• Sample name – the user can enter a sample name• Measurement Mode – see Chapter 7.2.3• Density for Start Temperature – see Chapter 10.1 and Chapter 7.2.4• Density for End Temperature – see Chapter 10.1 and Chapter 7.2.4• Lovis Capillary – see Chapter 7.1.1Optionally, the following Lovis parameters can be added to the quick settings.How to add or remove parameters to/from the quick settings is described in theInstrument Firmware Manual.C72IB002EN-E 349

Appendix D: Lovis ParametersParameter Type Parameter Select DescriptionTemperatureTemperatureEquilibrationUse Manual AngleAngleMeasurementCyclesUse VariationCoefficientVariation CoefficientMeasuring DistanceMax. MeasuringCyclesMeasuring TimeoutLongShortAutomaticTo set the measuringtemperature. Enter a value in the range from5.00 °C to 100.00 °C.To set a temperatureequilibration time before startingwith the measurement. Enter a value in the range from0 s to 999 s.To apply an entered measuringangle.To define the measuring angle.Enter a value in the range from15° to 80°.To define the amount ofmeasurement cycles. Enter avalue between 1 and 10.To apply an entered variationcoefficient.To define the variationcoefficient. Enter a value in therange from 0.1 % to 9.99 %.To measure with a distance of100 mm.To measure with a distance of25 mm.To automatically choose theoptimum measuring distance.To define the maximum value ofmeasuring cycles. Enter a valuebetween 4 and 20.To set a time limit within whichthe ball must have passed allthree coils of the Lovis capillaryblock during a run. If thiscriterion is not fulfilled, you getan error message. Enter a value in the range from60 s to 1700 s.350 C72IB002EN-E

Appendix D: Lovis ParametersAuto-distancethresholdAuto-anglethresholdLovis AlcoholSourceAlways activeApproximateApparent Extractor Manual InputTo set the runtime forautomatically using the shortmeasuring distance. Enter avalue in the range from 20 s to1700 s.To set the runtime forautomatically using a flattermeasuring angle. Enter a valuein the range from 10 s to 60 s.see Chapter 7.2.5.5C72IB002EN-E 351

Appendix E: Measuring under Special ConditionsAppendix E: Measuring under SpecialConditionsE.1 Measuring at High Humidity/Low TemperatureConditionsIf the ambient air contains humidity and the measuring temperature is lower thanthe ambient temperature, condensation could occur in the capillary when blowingthrough air for drying. Consequently, the capillary is not really dry. The humiditygets mixed with the filled sample and causes faulty measurement results.The higher the difference between the set measuring temperature and ambienttemperature and the higher the air humidity, the easier condensation occurs.To prevent condensation in the capillaryUse a drying cartridge (Mat. No. 65085) and connect it to the "DRY AIR IN AIRPUMP" connector at the rear of the instrument.NOTICE• Never connect hoses containing liquids or moist gases to the "DRY AIR INAIR PUMP" connector as this may lead to condensations in the air pumpand consequently damage the pump.• Operate the "DRY AIR IN AIR PUMP" connector only at ambientpressure.DRY AIR INAIR PUMPdryingcartridgeFig. E - 1Drying cartridge connectedFor a measuring temperature of 20 °C, a drying cartridge should be used underthe following conditions:Ambient temperature Relative air humidity (r. h.)20 °C > 70 %25 °C > 50 %30 °C > 38 %352 C72IB002EN-E

Appendix E: Measuring under Special ConditionsTo regenerate moist ruby gel / indicator gelThe drying cartridge contains beaded ruby gel, a non-toxic drying agent, or abeaded indicator gel.Color changes:Ruby gel: When active, the color of the drying agent is red. Ruby gel which hasabsorbed liquid turns orange.Indicator gel: When active, the color of the drying agent is orange. If the dryingagent has absorbed liquid, the color turns green.Moist drying agent can be regenerated: Pour it into a glass bowl and blow hot, dryair (max. 130 °C / 266 °F) through it for approx. 5 hours or place it in a laboratoryoven for a few hours (or overnight) until it is red respectively orange again.NOTICEDo not use higher drying temperatures than 130 °C (266 °F). Otherwise theindicator function of the gel is spoiled.E.2 Measuring in Harsh Environments• Use the keys below the touch screen for operating the buttons in the lowerarea of the screen.• Use a stylus or any convenient plastic stick with a soft and round ending tooperate the touch screen.• Use a PC mouse.Protection foil for touch screenYour Lovis 2000 M is delivered with a transparent plastic protection foil coveringthe touch screen. Three spare protection foils can be ordered (Mat. No. 81402).If the touch screen has become dirty, you can exchange the used protection foilagainst a new one.To exchange the protection foil of the touch screen1. Remove the used protection foil from the display using your finger nails.2. Hold the new foil in one hand and use the thumb finger nail of the other handto peel off the back liner from a small area.3. Position the back liner free end of the foil on one edge of the display, hold theopposite end and bend the foil backwards.C72IB002EN-E 353

Appendix E: Measuring under Special Conditions4. Peel off the back liner with one hand, simultaneously rolling the foil slowlyonto the display with the other hand.5. Carefully flatten out the foil with a clean cotton cloth, pushing bubblestowards the edges.In case of bubbles between foil and display, gently push them towards the edgesor lift up the foil partly or completely and reapply the foil more slowly.Protective cover for keyboard (Mat. No. 13350)If you use an optional external keyboard (Mat. No. 80807 or 80809), attach thiscover to prevent getting dust, dirt or spills into your keyboard. See also theproduct description list (PDL) supplied on the USB flash drive.Protective cover front (Mat. No. 88856; pack of 3 pcs.)To protect the complete front - touch display and soft keys - of your Lovis 2000 M(or DMA M/DSA M) from spills and dirt in harsh environment, attach a protectivecover front.The covers come completely with adhesive transfer tapes and can easily beslipped onto the instrument’s front.Fig. E - 2Attaching the protective cover front354 C72IB002EN-E

Appendix F: Trouble ShootingAppendix F: Trouble ShootingF.1 Error Messages and WarningsWarnings or error messages can be indicated or shown:• within the main window under "Lovis Condition" or, in combination with adensity meter/sound velocity meter under "Master Condition".• in the data memory browser• within the results file or printout (measurement results, adjustment results)• in a separate windowWarnings / error messages concerning a measurement in progressIf problems come up during a measurement in progress, errors or warnings aredisplayed in the main window. They are also part of the results file. Details can beviewed in message list.21Fig. F - 1Example Warning1 ... the yellow field indicates a warning; short text message2 ... the yellow sign indicates a warning. Tap the button to open the messagelist. See Fig. F - 3.21Fig. F - 2Example: Error1 ... the red field indicates an error; short text message2 ... the red sign indicates an error. Tap the button to open the message list.C72IB002EN-E 355

Appendix F: Trouble ShootingThe message list gives more information on warnings and errors:Fig. F - 3Example view of warning and error in message listThe messages tell you:• For which sample the problem occurred• Which kind of problem occurred• Item of the measuring assembly where the problem came up (e.g. LovisModule)Warning and error signs within the data memory browser and result fileFig. F - 4Error status displayed in data browserThe symbol indicates if you aborted a measurement by tapping . Thedata browser shows date, time and sample name (if any) of that test.The warnings and error messages displayed on the screen are also part of theresult file. They are shown under "Master Condition". See below:Fig. F - 5Error displayed in result output file356 C72IB002EN-E

Appendix F: Trouble ShootingError messages / warnings coming up in a separate windowIf errors or warnings occur outside a measurement, they come up in a separatewindow as shown in the following screenshot. Such a window contains always:• The text "Error" or "Warning" and the respective number• The error messageFig. F - 6Example error not related to measurement in progressError messages within the results of an adjustmentFig. F - 7Example error message in adjustment resultsC72IB002EN-E 357

Appendix F: Trouble ShootingF.2 List of error messagesMessage Problem Solutionmeasurement errorsFW/BW deviation too highThe forward/backward deviation ishigher than the allowable value forthe selected measuring angle.• Bubbles or contaminations inthe capillary.• Instrument position on labdesk is tilted.• Check the capillary and thefilling.• Place the instrument inhorizontal position andperform a level adjustment.EXPLANATION FW/BW deviation:Lovis 2000 M/ME calculates the average value of the forward and backward runs of a measurement.If the instrument is not in a level position and if no level adjustment has been performed, the calculated averageis faulty due to the differing forth and back runtimes. To get a valid measuring value that fault must be smallerthan 0.1 %. This corresponds to a tilted position of 2.5°. The internally set FW/BW deviation is the limit whichmust not be exceeded to ensure that the resulting fault in the average runtime value is smaller 0.1 %.Flat measuring angles (inclination angles) are less influenced by a tilted position than steep measuring angles.Therefore the FW/BW deviation limit is not constant but a curve over the angle range:FW/BW deviation limit [%]Fig. F - 8Forward / backward deviation limit depending on the inclination angleAn air bubble or particle in the capillary which slows down the ball in one direction (sitting in front of the ball) canalso lead to differing forth and back runtimes.Runtime too shortThe runtime is shorter than 2.5 sfor the short distance and shorterthan 10 s for the long distance.For normal measurements:The sample viscosity is too low forthe selected measuring angle andcapillary diameter.• Measure at a flatter angle• Use capillary with smallerdiameterFor adjustments:• Use a standard liquid withhigher viscosity358 C72IB002EN-E

Appendix F: Trouble ShootingMessage Problem Solutionmeasurement errorsUnstableNo coil reachedBall blockedErrorThe set value for the variationcoefficient was always exceededwithin the maximum number ofmeasurement cycles.No ball could be detected beforemeasuring timeout was reached.A timeout limit was exceeded afterone or more valid detections (ballpassing a coil) during the currentmeasurement .• Check the capillary and thefilling.• Change the variationcoefficient to a higher value.• Increase the maximum no. ofmeasurement cycles• Check whether there is a ballinside the capillary.• The ball got stuck somewhereinside the capillary. Check thecapillary and the filling.• For highly viscous fluids theball might be close to thecapillary end and not yet havepassed a coil.• The measuring timeout setting(Chapter 7.2.5.3) is unsuitablefor the sample’s viscosity. Seta time at least 4 times longerfor this measurement.• Check the capillary and thefilling. An air bubble meetingthe ball might have stopped itin the middle of the capillary.• The ball got stuck to the filingadapter due to an air bubble.Check the capillary and thefilling.• The set measuring timeout islong enough for the ball toreach at least one coil, but notlong enough for themeasurement as such. Set asuitable measuring timeout forthis sample (Chapter 7.2.5.3).• One of the coils might bedefective. If the error persists,contact your Anton Paarrepresentative.Date and time are not correct.Error 13• The date and time are not set.• The instrument has lostinternal settings after aninstrument firmware update.• The internal battery is weak ordefective (settings get lostwhenever switching off).The instrument firmware of themaster instrument (DMA M/DSA M) does not supportLovis 2000 ME microviscometermodule.• Set the current date and time(see Appendix C or InstrumentFirmware Manual).• If the error message persistsafter setting date and time,contact your Anton Paarrepresentative for batteryreplacement. Adjustment dataare not lost when replacing thebattery.Update the instrument firmware ofthe master instrument to the latestversion (comes on the suppliedUSB flash drive).C72IB002EN-E 359

Appendix F: Trouble ShootingF.3 Problems and SolutionsTable F.1: MeasurementProblem Cause and Correction SeeI can not start anymeasurement 15 minutes ormore after booting theinstrument.Verify whether one or more modules are using olderfirmware versions than expected from theinstrument’s firmware. This information is eitherdisplayed at the start screen or in the diagnosis listof the quick access area. Tap and select"Service > Update > Module Firmware" to updatethe respective modules using the internal firmwareupdate package.Table F.2: Touch screenProblem Cause and Correction SeeThe screen elements do notreact when being tapped.The screen flickers.Bad touch screen calibration: Perform a new touchscreen calibration.Due to manufacturing tolerances of the hardwarethe screen may flicker for certain values of areduced display brightness.Change the display brightnessInstrumentFirmwareManualInstrumentFirmwareManualInstrumentFirmwareManualTable F.3: Data memoryProblem Cause and Correction SeeI can not see certain resultsin the data memory.I have deleted all results butthere are still results visible inthe data memory.The filter function is activated and the results do notmatch the filter criteria: Change the filter settings.The filter settings in the "Delete Measured Data"menu are different from the settings in the "DataBrowser Settings" menu: Perform correspondingsettings in both menus.InstrumentFirmwareManualInstrumentFirmwareManualTable F.4: Data exportProblem Cause and Correction SeeThe export to the USB flashdrive does not work.Wrong output data areexported.The USB flash drive has the old super floppyformatting and is therefore not accepted by theinstrument: Reformat to FAT 32 or try a newer USBflash drive.The settings in the "Result Output" menu arewrong: Change the settings.InstrumentFirmwareManual360 C72IB002EN-E

Appendix F: Trouble ShootingTable F.5: Password protectionProblem Cause and Correction SeeThe instrument boots upwithout logon procedure andno password is requested.A user with activated auto logon function was loggedon the instrument before it was switched off: Tap onthe user indicator icon and log on with another useraccount.Table F.6: Printout problemsProblem Cause and Correction SeeNo printout on office printer. Office printer problems: Check if the printer hasenough paper, toner etc. See the respective printerinstruction manual.No printout on POS printer.The POS printer printoutmakes no sense.Wrong output values areprinted.The office printer type is not supported by yourinstrument.Wrong interface used: Use the interface which wasdefined in the "Printers Management" menu orchange the interface settings.Wrong or no definition of the printer in the "PrintersManagement" menu: Change the definition.Printer problems: Check the printer. See therespective printer instruction manual.Wrong or no definition of the printer in the "PrintersManagement" menu: Change the definition.Chinese printer: Declare the printer as "RS-232(Chinese)" in the "Printers Management" menu.Wrong communication settings on the POS printer:Change the DIP switch settings (see the printerinstructions).The settings in the "Result Output" menu arewrong: Change the settings.InstrumentFirmwareManualInstrumentFirmwareManualInstrumentFirmwareManualInstrumentFirmwareManualInstrumentFirmwareManualInstrumentFirmwareManualAppendixA.5InstrumentFirmwareManualC72IB002EN-E 361

Appendix G: Instrument Firmware Versions, Instruction ManualsAppendix G:Instrument FirmwareVersions, InstructionManualsInstruction Manuals - Version HistoryInstrumentFirmwareReleasedateDocument numberof InstructionManuals2.21 build 26 11.2011 C72IB001EN-AC72IB002EN-AChangesInstruction manual, printed, english, first releaseInstruction manual for measuring assemblies, pdf,english, first release2.21 build 37Hotfix01.2012 No new manuals. No changes.2.21 build 51 03.2012 C72IB001EN-BC72IB002EN-BXPAIB001EN-AOriginal instructions, printed, english, shortversion.Reference manual, pdf, english. Providessupplemental information for the operation ofLovis 2000 M/ME and the installation andoperation of the measuring assemblies.Instrument firmware manual platform M, firmwareversion 2.21 build 51, pdf, english.2.30 build 231 05.2012 C72IB001EN-CC72IB002EN-CXPAIB001EN-BOriginal instructions, printed, english, shortversion.Reference manual, pdf, english. Providessupplemental information for the operation ofLovis 2000 M/ME and the installation andoperation of the measuring assemblies. Extendedby installation instructions for measuringassemblies with Alcolyzer ME, HazeQC ME andpH ME.Instrument firmware manual platform M, firmwareversion 2.30 build 231, pdf, english.07.2012 C72IB001EN-DC72IB002EN-DOriginal instructions, printed, english, shortversion.Reference manual, pdf, english. Existing chaptersrevised. Extended by installation instructions formeasuring assemblies with AbbematPerformance/Performance Plus.362 C72IB002EN-E

Appendix G: Instrument Firmware Versions, Instruction ManualsInstrumentFirmware2.30 build 245HotfixReleasedateDocument numberof InstructionManuals10.2012 No new manuals. No changes.Changes2.32 build 32 11.2012 C72IB001EN-EC72IB002EN-DXPAIB001EN-BOriginal instructions, printed, english, shortversion.Reference manual, pdf, english. Existing chaptersrevised. New: Short capillary, polymercalculations, installation instructions formeasuring assemblies with Xsample 352/452 andAbbemat Performance/Performance Plus.Instrument firmware manual platform M, firmwareversion 2.30 build 231, pdf, english.Instrument Firmware - Version HistoryThis table lists only firmware changes relevant for Lovis 2000 M/ME. For the complete tableof changes consult the Instrument Firmware Manual Platform M.VersionChanges2.21 build 26 First release supporting Lovis 2000 M/ME microviscometer.2.21 build 37HotfixLovis utility Shake test: changed from 50.000 to 500 cycles (500 cycles ~ 16 minutes)2.21 build 51Hotfix- Fixed use of Var. Coeff. for evaluating measurement repetitions.- Changed auto angle range from full range to adjusted range only.- Fixed find min/max angle calculations in firmware.- Changed waiting times after measurement cycles.- Fixed water check reference.- Improved adjustment procedure. Change of error messages and constant limits.- Fixed level adjustment cancellation when capillary detection is disabled- Changed variation coefficient calculation to use Bessel Correction.- Improved calculation of progress-information while running shake test, adjustments and measurement.- Fixed detection of fan failure. (Heat sink temperature >80°C)- Fixed error in export of capillary systems when more than one printer is installed.- Changed set shake test cycles in final inspection procedure to 10.C72IB002EN-E 363

Appendix G: Instrument Firmware Versions, Instruction Manuals2.30 build 231 - New single point adjustment allows adjusting a capillary system only at one angle.- Added new redo functionality for the verification measurement of all angle adjustmenttypes.- New input option for extract value to enable more precise beer/wort application results.- Added dynamic viscosity into check measurement result view. New input option for Lovisdensity source allows choosing manual input or function instead of DMA.- Added support of measurement modes "Repeated Mode" and "Temperature Table Scan"with Lovis.- New input option to enter exact density by starting custom checks with Lovis withoutDMA.- Added the sample name to the result output by default to all Lovis standard methods.- Support of Abbemat Performance Line, HazeQC ME, Alcolyzer Beer and pH moduleswith Lovis.- Measurement results will be displayed even if a warning occurred during measurement.- Inverted order of angles in standard and extended adjustments from ascending todescending.- Changed hold time before starting verification measurements in adjustments to 90 s.- Using dynamic degree of polynomial function in angle adjustments (minimum 3 points).- Changed user interface texts (Meas. Length > Meas. Distance, Cap. Diameter incapillary management; Max. Std. Dev. > Max. Var. Coeff. in result page of angleadjustments).- Removed high frequent whirring caused by Lovis block fan. Fixed bug in writing I-ButtonEPROM by using strong pull up resistor.- Removed bug in user interface if measuring scans were stopped.2.30 build 245Hotfix- Handling of grey implemented digits in predetermination mode fixed.- Errors during expanding the quick access area resolved.- Problems with mandatory data fields in combination with auto air check resolved.- Lovis Adjustment Verification procedures improved.- Lovis Level adjustment improved.- Redo function in all Lovis adjustments fixed.- Problem with Lovis Utility "Fan Service" fixed.- German translations improved.- Sample volume for Lovis with Xsample52 auto sampler revised.- Inhibition of cleaning auto sampler system with Lovis and Abbemat removed.- Simultaneous operation of DSA, DMA HP and Lovis ME supported.- Start-up problems of DSA M with Lovis ME in temperature scan mode fixed.2.32 build 32 - Added fully support of Polymer Solutions.- Support of short distance capillary systems for Low Volume Applications.- Implemented graphical data visualization for certain measurement modes.- Improved Level Adjustment procedure.- Introduced new measurement mode Zero Shear Scan.- Approximate calculation of shear rate although capillary is not adjusted.- Showing more detailed information while capillary adjustment.- Fixed Level Adjustment data memory.- Removed bug in Max. Angle Calculation- Fixed Lovis Fan Service.- Improvements in the user interface and data grid visualization.364 C72IB002EN-E

Appendix G: Instrument Firmware Versions, Instruction ManualsC72IB002EN-E 365

IndexIndexSymbols button 31 button 31 button 31 button 31 button 31AAbbemat 263, 275, 276, 297, 306accessory kKit 116, 120accuracy 59adapterLuer 1/4" UNF 118Luer cone 118UNF/Hose 118UNF/screwed tube 118UNF/UNF 118adjust the capillary 318adjustmentadjustment data 68definition of 57ball 62level 61, 318, 319resetting to factory adjustment 68single point 319temperature 66adjustments 319air hose 311air humidity 332, 352air preparation set dew point -40°C 313air preparation set w/o air dryer 310air pumpoutlet 27Alcolyzer ME 220, 234, 242, 247audit trail function 20automatic distance 52Bbackup file 107backup of instrument settings 107ball dispenser 117balls 118beer and wort settings 54, 56brush 119brushinga capillary 92a short capillary 96Ccable clip 119, 121, 140, 144, 156,165, 179,206, 223, 235, 247, 251, 255cables 40calibrationdefinition of 57CANbooster 219, 263interface 28, 30interface cable 40, 115power supply 135, 234, 246capillary 117, 342, 344, 346adjustment 63block 28brushing 92conditioning 92creating 317range 329filling 70import adjustment 62inserting 77special cleaning 100checkdefinition of 57history 60hoses 102,324measurement 48settings 58time interval 58checks 323chemical compatibility 340chemical resistant hoses 158, 161, 172, 174,199, 202, 214, 216compressed air 310condensation 352conditioningthe capillary 92, 317the short capillary 96core range 329crush protection 115, 128Ddata management 20date and time 60density checkcheck results 60check/SOP data 60performing 60performing a density check 59density settings 53366 C72IB002EN-E

Indexdevice software version 316displaybuttons 31clock 30content area 31output field 31progress bar 31quick access buttons 31sample number 30user indicator 30DMA M 177, 183DMA M / DSA M 99, 199, 202, 323DMA M /DSA M 205DMA M/DSA M 188, 193, 209, 214, 216drain nozzle 131drip plate 17, 122, 130drying cartridge 315, 352Eedge protection 115, 127errorball probably blocked 359date and time are not correct 359FW/BW deviation too high 358no ball detected 359runtime too short 358unstable 359error 13 359error status 32ethernet interface 28extended adjustment 319extended range 329Ffilling with a syringe 84filling adapter UNF front side 117Filling and cleaning adapter 117filling options 147filling support 28firmwareupdate 109version 108versions 22fixed angle scan 49formula parameters 50funnel 117fuse 120fuse holder 28HHazeQC ME 220, 234, 242, 247, 297high humidity/low temperature conditions 352hook for O-ring removal 119horizontal setup 233hose 118, 137, 3251.6x3.2 Tygon 119220x3x2 FEP 1182x4 Silicone 1192x4 Viton 119300x3x2 FEP 119350x3x2 FEP 1193x5 Silicone 119530x3x2 FEP 119600x3x2 119clamp 131connections 139, 143, 177, 183connector 325low volume 124Iimproved chemical resistance 338injection adapter 118, 325inspection window 27, 29instrument 109firmware 109status 32interfaceUSB 27, 28, 30CAN 28, 30CAN cable 40, 115RS-232 28Jjack 231, 240, 295KKalrez® 103, 124Llanguage pack 109leak test 99, 102,,323leak tightness 324leakiness 103, 325, 326, 327LED 29level adjustment 61, 318, 319live camera view 32live view of sensor signals 105long distance 52Lovis 2000 M Base Unit 114Lovis 2000 ME 113Lovis methodsLovis / DMA 46Lovis extended 46Lovis Polymer 47Lovis standard 46C72IB002EN-E 367

Indexlow volume 137, 141, 181hoses 181magazine 181Luer plug 117Mmagazine for 6 mL vials 124main menu 31main screen 30mains cable 26, 114maintenance 323master instrument 112maximum air pressure 309, 312measurementdata 318errors 358mode settings 48modes 48trouble shooting 360measuringcell 32cycles 53methods 45parameter 51range 328, 332temperature 51, 59, 352menuaccessing 33current position in 33options 33message list 355method settings 45, 317micro flow cell 264, 268microviscometer 20min. volume 330module 112module firmware 109module housing Lovis ME 114, 137, 142, 147,163, 176, 182, 186, 204, 220, 255monitor mode 32Mounting the air preparation kit 311, 314Multi fill measurement 49Multiple measurement 48Nnavigation path 33OO-ring 103, 325O-Ring 5x1.5 103, 116Pparameter for polymer solutions 54peristaltic pump 147, 323pH ME 220, 234, 243, 251, 255, 297plug 231, 240, 294polymer 49angle count 55molar mass calculation 56settings 55solutions 44solvents administration 44powercord 40, 114LED 316switch 28precision 328pressure 147pressure regulator 312protection foil 26, 98, 114, 353pump hose 323Rreference manual 12remove Tygon® hose 155, 170, 190, 228repeated mode measurement 48restoring instrument settings 108retrofit kitchemical 123petro 123rolling ball principle 23RS-232 interface 28ruby gel 353Ssafetycover 128messages 13waste container 125sample status 89sample vessel elevated 146, 184sample volume 330samples 21screw cap 116screwdriver 120sealing gasket 134select a method 316serial number 30set of balls 118setting the pressure 312setting up the hardware 126short capillarybrushing 96conditioning 96short distance 52368 C72IB002EN-E

Indexsingle point adjustment 319sleeve nut 231, 240, 294slot 38soft keys 27stand 233adjustable feet 233for Xsample 125for measuring assembly 125standard adjustment 319standard hoses 149, 153, 164, 168, 188, 193,205, 209standard measurement 48start-up 316status LED 316sticker 120, 124suction 147supplied parts 113supported combinations 111syringe 116Ttemperature 60adjustment 66probe 65range 328scan 49temperature table scan 49terminating resistor 40, 115, 222, 235, 242, 246,256, 269, 275, 282, 290thermometer specification 64time scan 49touch screen 27, 332tube 119tube holder 151typographical conventions 14Wwastebottle 114, 134bottle glass 123container 125container 8 L HDPE 132, 281, 297hose 141water check 59wetted parts 334XXsample 122 147, 153, 158, 161, 188, 193, 199,202Xsample 22 164, 168, 172, 174, 205, 209, 214,216Xsample 352/452 137, 177Xsample 52 142, 183ZZero Shear Scan 49UUSB flash drive 117, 121USB interface 28user function 54U-View TM 32Vvertical setup 225VGA interface 28Visual inspection 99, 323Viton® 103Viton® Extreme 116voltage 332C72IB002EN-E 369

Index370 C72IB002EN-E

List of FiguresList of FiguresFig. 2 - 1 Drip Plate and Stand Measuring Assembly ..................................................................... 17Fig. 2 - 2 Symbol "Hazard" on the Lovis capillary block ................................................................. 18Fig. 3 - 1 Lovis 2000 M and Lovis 2000 ME with DMA M and Xsample ......................................... 20Fig. 3 - 2 Lovis measuring principle ................................................................................................ 23Fig. 3 - 3 Standard capillary and accessories for manual filling ...................................................... 24Fig. 3 - 4 Standard capillary and accessories for flow-through filling .............................................. 24Fig. 3 - 5 Short capillary and accessories for low sample volume .................................................. 24Fig. 5 - 1 Lovis 2000 M front and left side ....................................................................................... 27Fig. 5 - 2 Lovis 2000 M right side .................................................................................................... 28Fig. 5 - 3 Lovis 2000 M rear view .................................................................................................... 28Fig. 5 - 4 Module Housing Lovis ME front view .............................................................................. 29Fig. 5 - 5 Module Housing Lovis ME right side view ....................................................................... 29Fig. 5 - 6 Module Housing Lovis ME rear view ............................................................................... 30Fig. 5 - 7 Main screen example – Camera view of U-tube only in combination with DMA 4500/5000 M ........................................................................................................... 30Fig. 5 - 8 Expanded quick access area ...........................................................................................31Fig. 5 - 9 Menu screen examples ................................................................................................... 33Fig. 6 - 1 Lovis 2000 M with air hose plus Luer cone connected .................................................... 35Fig. 6 - 2 Removing the cover of a DMA M/DSA M ........................................................................ 37Fig. 6 - 3 Removing the bottom plate of a DMA M/DSA M ............................................................. 37Fig. 6 - 4 Inserting the Lovis 2000 ME Microviscometer Module .................................................... 38Fig. 6 - 5 Mounting the Lovis 2000 ME module .............................................................................. 38Fig. 6 - 6 Taking apart the left rear foot of Module Housing Lovis ME ............................................ 39Fig. 6 - 7 Assembling the left rear foot of Module Housing Lovis ME ............................................. 39Fig. 6 - 8 Connecting the cables for Module Housing Lovis ME ..................................................... 40Fig. 7 - 1 Example Method Lovis standard ..................................................................................... 46Fig. 7 - 2 Example Method Lovis detailed ....................................................................................... 46Fig. 7 - 3 Example Method Lovis / DMA ......................................................................................... 46Fig. 7 - 4 Example Method Lovis Polymer (Single Conc.) .............................................................. 47Fig. 7 - 5 Example Method Lovis Polymer (Multi Conc.) ................................................................. 47Fig. 8 - 6 Inserting the Pt-100 temperature probe ........................................................................... 65Fig. 8 - 7 Temperature probe and adapter - recess fitting into the clip ........................................... 65Fig. 8 - 8 Connecting the temperature probe with the MKT 50 ....................................................... 67Fig. 8 - 9 Mean value on MKT 50 display (Channel 1) – Example ................................................. 68Fig. 9 - 1 Mounting the Filling Adapter Luer Female ....................................................................... 70Fig. 9 - 2 Inserting a ball with tweezers and funnel or ball dispenser ............................................. 70Fig. 9 - 3 Empty capillary with two adapters and ball (see arrow) .................................................. 70Fig. 9 - 4 Drawing in sample from a vessel ..................................................................................... 71Fig. 9 - 5 Filling the capillary from below ........................................................................................ 71Fig. 9 - 6 Removing air bubbles by knocking with a finger ............................................................. 72Fig. 9 - 7 Inserting the plug ............................................................................................................. 72Fig. 9 - 8 Fastening the Luer plug ................................................................................................... 73Fig. 9 - 9 Filled Capillary with ball (see arrow) ................................................................................ 73Fig. 9 - 10 Mounting the Filling Adapter Luer Female ....................................................................... 74C72IB002EN-E 371

List of FiguresFig. 9 - 11 Filling a microliter syringe with sample ............................................................................74Fig. 9 - 12 Filling the short capillary with a microliter syringe ........................................................... 75Fig. 9 - 13 Checking for and removing air bubbles ........................................................................... 75Fig. 9 - 14 Inserting a ball with funnel or directly .............................................................................. 75Fig. 9 - 15 Pushing the ball into the capillary .................................................................................... 76Fig. 9 - 16 Inserting the hollow plug into the short capillary .............................................................. 76Fig. 9 - 17 Filled short capillary with ball (see arrow) and hollow plug .............................................. 76Fig. 9 - 18 Capillary twist protection .................................................................................................. 77Fig. 9 - 19 Recess for twist protection .............................................................................................. 77Fig. 9 - 20 Mounting the screw caps closed ..................................................................................... 77Fig. 9 - 21 Mounting the Filling Adapter UNF on the front side of the capillary ................................ 78Fig. 9 - 22 Capillary twist protection .................................................................................................. 78Fig. 9 - 23 Recess for twist protect. .................................................................................................. 78Fig. 9 - 24 Installing the flow-through system ................................................................................... 79Fig. 9 - 25 Flow-through system ready for hose connection, front and back side ............................ 79Fig. 9 - 26 Lovis 2000 M flow-through filling – Items listed below .................................................... 80Fig. 9 - 27 Hose for Filling Support – DSA 5000 M ........................................................................... 81Fig. 9 - 28 Hose DSA 5000 M to capillary block without / with Filling Support ................................. 81Fig. 9 - 29 Connection inside the Filling Support .............................................................................. 82Fig. 9 - 30 Hose Lovis capillary block to waste bottle ....................................................................... 82Fig. 9 - 31 Lovis 2000 M – Flow-through filling with a syringe .......................................................... 83Fig. 9 - 32 Lovis 2000 ME with DMA M – Flow-through filling with a syringe ................................... 84Fig. 10 - 1 Method Lovis /DMA – Example: Measurement in progress ............................................ 87Fig. 10 - 2 Method Lovis detailed – Example: Measurement finished .............................................. 88Fig. 11 - 1 Drying a capillary with the air pump ................................................................................. 92Fig. 11 - 2 Brushing a capillary ......................................................................................................... 93Fig. 11 - 3 Fixing a hollow needle to the Adapter Luer cone of the air hose ..................................... 94Fig. 11 - 4 Drying a short capillary with the air pump ........................................................................ 94Fig. 11 - 5 Rubbing the ball with tissue paper ................................................................................... 96Fig. 12 - 1 Interdental brush / inserting it into the hollow needle 1.2 .............................................. 101Fig. 12 - 2 Pushing the interdental brush into the hollow needle 1.2 .............................................. 101Fig. 12 - 3 Pushing the interdental brush into the dead end of the capillary ................................... 101Fig. 12 - 4 Removing the O-rings from a filling adapter .................................................................. 103Fig. 15 - 1 Lovis 2000 ME + DMA M + Xsample 122 ...................................................................... 111Fig. 16 - 1 Accessory Kit Complete - Lovis ..................................................................................... 116Fig. 16 - 2 Accessory Kit Basic - Lovis ........................................................................................... 120Fig. 17 - 3 Mounting the edge protection ........................................................................................ 128Fig. 17 - 4 Mounting the crush protection ....................................................................................... 129Fig. 17 - 5 Drip plate - supplied parts .............................................................................................. 130Fig. 17 - 6 Attaching the drip plate onto the master instrument ...................................................... 131Fig. 17 - 7 Connecting the waste hose to the drip plate ................................................................. 131Fig. 17 - 8 Connecting the waste hose to the waste container ....................................................... 132Fig. 17 - 9 Waste container - supplied parts and connectors ......................................................... 133Fig. 17 - 10 Replacing the sealing gasket for the waste container/waste bottle ............................... 135Fig. 17 - 11 CAN power supply ......................................................................................................... 135Fig. 18 - 1 Lovis + Xsample 452 hose connections ........................................................................ 139Fig. 18 - 2 Lovis + Xsample 352/452: mounting the hose between needle and Lovis inlet ............ 140Fig. 18 - 3 Lovis + Xsample 52 hose connections .......................................................................... 143372 C72IB002EN-E

List of FiguresFig. 18 - 4 Lovis + Xsample 52: Connecting the sample filling hose .............................................. 145Fig. 18 - 5 Lovis + Xsample 52: sample vessel on elevated position ............................................. 146Fig. 18 - 6 Lovis Module Housing ME, mounting the cable clips .................................................... 147Fig. 18 - 7 Lovis + Xsample 122, standard hoses, pressurized filling ............................................. 149Fig. 18 - 8 Connecting the hose between pump hose and Lovis .................................................... 151Fig. 18 - 9 Lovis + Xsample 122 standard hoses, filling by suction ................................................ 153Fig. 18 - 10 Mounting the hose between Xsample needle and Lovis ............................................... 155Fig. 18 - 11 Optional splitting the waste hose ................................................................................... 155Fig. 18 - 12 Lovis + Xsample 122, chemical resistant hoses, pressurized filling .............................. 158Fig. 18 - 13 Lovis + Xsample 122, chemical resistant hoses, filling by suction ................................ 161Fig. 18 - 14 Lovis + Xsample 22, standard hoses, pressurized filling ............................................... 164Fig. 18 - 15 Lovis + Xsample 22 - connecting the FEP hose to Lovis .............................................. 166Fig. 18 - 16 Lovis + Xsample 22, standard hoses, filling by suction ................................................. 168Fig. 18 - 17 Lovis + Xsample 22 - connecting the Tygon hose between needle and Lovis .............. 170Fig. 18 - 18 Lovis + Xsample 22, chemical resistant hoses, pressurized filling ................................ 172Fig. 18 - 19 Lovis + Xsample 22, chemical resistant hoses, filling by suction .................................. 174Fig. 19 - 1 Lovis + DMA M + Xsample 352/452 hose connections ................................................. 177Fig. 19 - 2 Lovis + DMA M + Xsample 452 - Connecting the hose between DMA M and Lovis ..... 179Fig. 19 - 3 Lovis + DMA M + Xsample 352/452 complete .............................................................. 180Fig. 19 - 4 Lovis + DMA M + Xsample 452 with low volume hoses, low volume magazine and conical 6 mL PS vials ............................................................................................. 181Fig. 19 - 5 Lovis + DMA M + Xsample 52 hose connections .......................................................... 183Fig. 19 - 6 Lovis + DMA M + Xsample 52: elevated sample vessel ................................................ 184Fig. 19 - 7 Lovis + DMA M + Xsample 52 - Mounting the hose between DMA M and Lovis .......... 185Fig. 19 - 8 Mounting the cable clips ................................................................................................ 187Fig. 19 - 9 Lovis + DMA M/DSA M + Xsample 122, standard hoses; pressurized filling ................ 188Fig. 19 - 10 Lovis + DMA M + Xsample 122 pressure filling - connecting the hose between Lovis and DMA .............................................................................................................. 191Fig. 19 - 11 Lovis + DMA M/DSA M + Xsample 122, standard hoses; filling by suction ................... 193Fig. 19 - 12 Lovis + DMA M + Xsample 122 suction filling - connecting the hose between Lovis and DMA .............................................................................................................. 195Fig. 19 - 13 Optional splitting the waste hose ................................................................................... 196Fig. 19 - 14 Lovis + DMA M + Xsample 122 complete ..................................................................... 197Fig. 19 - 15 Lovis + DMA M/DSA M + Xsample 122, chemical resistant hoses, pressurized filling ........................................................................................................... 199Fig. 19 - 16 Lovis + DMA M/DSA M + Xsample 122, chemical resistant hoses, filling by suction ............................................................................................................. 202Fig. 19 - 17 Lovis + DMA M /DSA M + Xsample 22, standard hoses, pressurized filling ................. 205Fig. 19 - 18 Lovis + DMA M + Xsample 22 - connecting the hose between Lovis and DMA ............ 207Fig. 19 - 19 Lovis + DMA M/DSA M + Xsample 22, standard hoses, filling by suction ..................... 209Fig. 19 - 20 Lovis + DMA M + Xsample 22 - connecting the hose between Lovis and DMA ............ 211Fig. 19 - 21 Lovis + DMA M/DSA M + Xsample 22, chemical resistant hoses, pressurized filling ... 214Fig. 19 - 22 Lovis + DMA M/DSA M + Xsample 22, chemical resistant hoses, filling by suction ...... 216Fig. 20 - 1 Assembly for beer and wort measurement .................................................................... 218Fig. 20 - 2 Placing master instrument and modules - vertical setup ............................................... 221Fig. 20 - 3 Connecting the cables and the CAN power supply - vertical setup ............................... 222Fig. 20 - 4 Positioning the cable clips (hose guidances) ................................................................. 223Fig. 20 - 5 Connecting the hoses - complete system, vertical setup .............................................. 225C72IB002EN-E 373

List of FiguresFig. 20 - 6 Assembling the hose between needle and inlet of master instrument .......................... 227Fig. 20 - 7 Hoses master instrument outlet to Alcolyzer ME ........................................................... 228Fig. 20 - 8 Hose Alcolyzer ME to Lovis 2000 ME inlet .................................................................... 229Fig. 20 - 9 Fitting the hose connected to Lovis 2000 ME rear side ................................................. 230Fig. 20 - 10 Mounting the plug to the inlet hose for the pH ME module ............................................ 231Fig. 20 - 11 Positioning the stand for horizontal setup of a measuring assembly ............................. 233Fig. 20 - 12 Placing master instrument and modules - horizontal setup ........................................... 233Fig. 20 - 13 Connecting the cables and the CAN power supply - horizontal setup ........................... 234Fig. 20 - 14 Mounting the cable clips on Lovis 2000 ME rear side and on HazeQC ME -horizontal setup ............................................................................................................. 235Fig. 20 - 15 Connecting the hoses - complete system, horizontal setup .......................................... 236Fig. 20 - 16 Hoses between master instrument outlet Alcolyzer ME and HazeQC ME outlet .......... 238Fig. 20 - 17 Hose between HazeQC ME and Lovis 2000 ME ........................................................... 239Fig. 20 - 18 Assembling the hose between Lovis 2000 ME and Haze QC ME ................................. 239Fig. 20 - 19 Connecting the hoses to pH ME .................................................................................... 240Fig. 20 - 20 Connecting the cables - DMA M + Lovis 2000 ME + HazeQC ME + pH ME + Xsample 122 .................................................................................................................. 242Fig. 20 - 21 All hoses connected - DMA M + Lovis 2000 ME +Alcolyzer ME + pH ME + Xsample 122 .................................................................................................................. 243Fig. 20 - 22 Connecting the cables - DMA M + Lovis 2000 ME + HazeQC ME + Xsample 122 ....... 246Fig. 20 - 23 All hoses connected - DMA M + Lovis 2000 ME + Alcolyzer ME + Xsample 122 ......... 248Fig. 20 - 24 Connecting the hose between Lovis 2000 ME and waste container ............................. 250Fig. 21 - 1 Lovis ME + DMA M + pH - connecting the hoses .......................................................... 252Fig. 21 - 2 Connecting the hose between DMA M and Lovis .......................................................... 254Fig. 21 - 3 Connecting the cables ................................................................................................... 256Fig. 21 - 4 Lovis ME + DMA M + pH + Xsample 122 - connecting the hoses ................................. 258Fig. 21 - 5 Connecting the hose between DMA M and Lovis 2000 ME .......................................... 260Fig. 21 - 6 Preparing the hose 600x3x2 .......................................................................................... 260Fig. 21 - 7 Guiding the hoses .......................................................................................................... 261Fig. 22 - 8 Lovis 2000 M + Abbemat - All hoses connected ........................................................... 266Fig. 22 - 1 Lovis 2000 M/DMA M/DSA M rear side - Mounting the cable clips ............................... 269Fig. 22 - 2 Lovis 2000 ME + DMA M + Abbemat - connecting the cables ...................................... 269Fig. 22 - 3 Lovis 2000 ME + DMA M + Abbemat - All hoses connected ......................................... 271Fig. 22 - 4 Connecting the hose between DMA M and Lovis .......................................................... 273Fig. 22 - 5 Connecting the cables ................................................................................................... 275Fig. 22 - 6 Lovis 2000 ME + DMA M + Abbemat + Xsample 122 - all hoses connected ................ 276Fig. 22 - 7 Connecting the hose between Lovis and DMA M .......................................................... 278Fig. 22 - 8 Mounting the cable clips onto Module Housing Lovis ME ............................................. 280Fig. 22 - 9 Lovis 2000 ME + DMA M + HazeQC ME + Abbemat + Xsample 122 - Placing master instruments and modules ...................................................................... 281Fig. 22 - 10 Lovis 2000 ME + DMA M + HazeQC ME + Abbemat + Xsample 122 - Connecting the cables ................................................................................................... 282Fig. 22 - 11 Lovis 2000 ME + DMA M + HazeQC ME + Abbemat + Xsample 122 - all hoses connected283Fig. 22 - 12 Preparing the hose between master instrument outlet and HazeQC ME inlet .............. 285Fig. 22 - 13 Mounting the hoses between master instrument outlet, HazeQC ME and Lovis capillary block front side ....................................................................................... 286Fig. 22 - 14 Preparing the hose between HazeQC ME and Lovis capillary block front side ............. 286374 C72IB002EN-E

List of FiguresFig. 22 - 15 Lovis 2000 ME + DMA M + pH ME + Abbemat + Xsample 122 - Placing master instruments and modules ..................................................................... 289Fig. 22 - 16 Lovis 2000 ME + DMA M + Abbemat + pH ME + Xsample 122 - Connecting the cables .................................................................................................. 290Fig. 22 - 17 Lovis 2000 ME + DMA M + Abbemat + pH ME + Xsample 122 - All hoses connected ...................................................................................................... 291Fig. 22 - 18 Connecting the hoses between Abbemat and pH ME ................................................... 294Fig. 22 - 19 Lovis 2000 ME + DMA M + HazeQC ME + pH ME + Abbemat + Xsample 122 - Placing master instruments and modules ...................................................................... 297Fig. 22 - 20 Lovis 2000 ME + DMA M + Abbemat + pH ME + Xsample 122 - All hoses connected ...................................................................................................... 298Fig. 22 - 21 Lovis 2000 ME rear side - Mounting the cable clips ...................................................... 303Fig. 22 - 22 Lovis 2000 ME + DMA M + Abbemat + Xsample 352/452 - Placing master instrument and modules ...................................................................... 304Fig. 22 - 23 Connecting the cables ................................................................................................... 305Fig. 22 - 24 Lovis 2000 ME + DMA M + Abbemat + Xsample 452 ................................................... 306Fig. 22 - 25 Lovis + Xsample 352/452: mounting the hose between needle and Lovis inlet ............ 308Fig. 23 - 1 Air preparation set without air dryer (Mat. No. 21903) ................................................... 310Fig. 23 - 2 Connecting air hoses to the distributor .......................................................................... 311Fig. 23 - 3 Connecting the pressure hose to Xsample 352/452 ...................................................... 312Fig. 23 - 4 Setting the correct air pressure ..................................................................................... 312Fig. 23 - 5 Air preparation set dew point -40 °C (Mat. No. 21902) .................................................. 313Fig. 23 - 6 Connecting hoses to the distributor of the air preparation kit -40 °C ............................. 314Fig. 23 - 7 Connecting pressure hose and drying cartridge to Xsample 352/452 ........................... 315Fig. E - 1 Drying cartridge connected ........................................................................................... 352Fig. E - 2 Attaching the protective cover front .............................................................................. 354Fig. F - 1 Example Warning ......................................................................................................... 355Fig. F - 2 Example: Error .............................................................................................................. 355Fig. F - 3 Example view of warning and error in message list ..................................................... 356Fig. F - 4 Error status displayed in data browser ......................................................................... 356Fig. F - 5 Error displayed in result output file ............................................................................... 356Fig. F - 6 Example error not related to measurement in progress ............................................... 357Fig. F - 7 Example error message in adjustment results ............................................................. 357Fig. F - 8 Forward / backward deviation limit depending on the inclination angle ........................ 358C72IB002EN-E 375