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Download pdf guide - VSN International

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15 Examples 247on male and female pups was also of interest. Three litters had to be droppedfrom experiment, which meant that one dose had only 7 dams. The analysismust account for the presence of between dam variation, but must also recognisethe stratification of the experimental units (pups within litters) and that dosesand littersize belong to the dam stratum. Table 15.2 presents an indicative AOVdecomposition for this experiment.Table 15.2 Rat data: AOV decompositionstratum decomposition type df or neconstant 1 F 1damsdose F 2littersize F 1dam R 27dams.pupssex F 1dose.sex F 2errorRThe dose and littersize effects are tested against the residual dam variation, whilethe remaining effects are tested against the residual within litter variation. TheASReml input to achieve this analysis is presented below.Rats exampledose 3 !Asex 2 !Alittersizedam 27pup 18weightrats.asd !DOPATH 1 # Change DOPATH argument to select each PATH!PATH 1weight ~ mu littersize dose sex dose.sex !r dam!PATH 2weight ~ mu out(66) littersize dose sex dose.sex !r dam!PATH 3weight ~ mu littersize dose sex !r dam!PATH 4weight ~ mu littersize dose sex

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