Oncology Probes

FISHgrade CotFISHgrade C 0 t DNA is extracted from human placental DNAtreated under conditions that enrich for repetitive DNAsequences (1,2). These conditions have been optimized byKreatech Diagnostics for the specific use in FISH experiments.FISHgrade C 0 t DNA suppresses cross-hybridization to humanrepetitive DNA. Amounts of FISHgrade C 0 t DNA needed in aFISH experiment should be determined empirically, but willbe in the order of 12.5 – 50 times excess to the amount oflabeled genomic DNA.• High quality human C 0 t-1 DNA optimized for use in FISHapplication• Specific quality control with FISH analysis• Works perfectly for probes labeled with FISHBright ULS-Kits.Cat.# KB-COT FISHgrade CotFISH image on metaphase spread performed using a classical FISHprobe labeled in red/green colors using a FISHBright labelingkit. FISHgrade C0t DNA has been used to block all aspecific DNAsequences. The probe specifically recognizes deletions at 20qchromosome that have been related to myelodysplastic syndromes.References1. Weiner, A.M., et al. (1986) Ann. Rev. Biochem. 55, 631.2. Britten, R.J., et al. (1974) Methods Enzymol. 29, 363.Ordering informationCat#FISHgrade Cot 500 μg KB-COTFISHBright Labeling Kits101