Oncology Probes

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Technical AppendixThe Poseidon Probe MapsAll locus specific probes are provided with a map showingthe genome region and/or genes the probe will cover. The STSmarkers indicating the most proximal and distal end coveredby the probe are added as well. Those markers can be used todescribe in situ hybridization results in accordance with theInternational System for Human Cytogenetic Nomenclature(ISCN, 2009). For more information see e.g. Genome Browserat genome.ucsc.edu or Ensemble at www.ensembl.orgRB1Most proximal marker covered by probeD13S147713q14 RFP2DLEU1D13S272650 KB220 KBTarget Gene (or Genes) covered by ProbeProbe size and color indication13D13S25450 KBProbe maps not to scaleMost distal marker covered by probeDistance to another region or gene of interestKreatech’s fluorophores and filter recommendationsStandard fluorophores used forPoseidon FISH probesFluorophore Color Excitation/EmissionPlatinumBright415 Blue 429/470PlatinumBright495 Green 495/517PlatinumBright550 Red 550/580Fluorophores used in PlatinumBright andFISHBright Labeling KitsFluorophore Color Excitation/EmissionPlatinumBright415 Blue 429/470PlatinumBright495 Green 495/517PlatinumBright505 Green 500/528PlatinumBright550 Red 550/580Technical Appendix112Fluorophores used in PGS kits(KBI-40050; 40051; 40060-40064)Fluorophore Color Excitation/EmissionPlatinumBright405 Dark Blue 410/455PlatinumBright415 Blue 429/470PlatinumBright495 Green 495/517PlatinumBright547 Light Red 547/565PlatinumBright590 Dark Red 587/612PlatinumBright647 Far Red 647/665PlatinumBright547 Light Red 547/565PlatinumBright570 Dark Red 570/591PlatinumBright647 Far Red 647/665When ordering a filter from Chroma or other suppliers pleasemake sure to either have a cube or slider available for yourspecific brand and type of microscope.Filters from other suppliers covering the same excitation andemission wavelengths as described above will also workperfectly with Poseidon probes. For further questions pleasecontact customerservice@kreatech.com
Recommended Filters:Single FiltersFluorophore Ex/Em Color Recommended Filter Excitation Filter Dichromatic Mirror Emission Filter Filter (Chroma cat#)DAPI DAPI DAPI / UV 360/20 400 LP 425 LP 11000PlatinumBright405 410/455 Dark Blue Blue 405/10 425 LP 460/50 31016 / 31037 / 31047PlatinumBright415 429/470 Blue Aqua 436/20 455 LP 480/30 31036v2PlatinumBright495 495/517 Green FITC / Green 480/30 505 LP 535/40 31001 / 41001PlatinumBright505 500/528 Green FITC / Green 480/30 505 LP 535/40 31001 / 41001PlatinumBright550 550/580 Red TRITC 540/25 565 LP 605/55 31002 / 41002PlatinumBright547 547/565 Light Red Cy3 / Gold 535/50 565 LP 610/75 31002 / 41002PlatinumBright570 570/591 Red Texas Red 560/40 595 LP 645/75 31004 / 41004PlatinumBright590 587/612 Dark Red Texas Red 560/40 595 LP 645/75 31004 / 41004PlatinumBright647 647/665 Far Red Cy5 / Far red 620/60 660 LP 700/75 41008Dual/Triple FiltersFluorophore Color Recommended Filter Excitation Filter Dichromatic Mirror Emission Filter Filter (Chroma cat#)PlatinumBright495/550 Green/Red Dual FITC/Cy3PlatinumBright 415/495/550 Blue/Green/Red DAPI/FITC/Cy3DAPI / PlatinumBright 495/550DAPI/Green/RedDAPI/FITC/TRITC or Cy3485/10563/12430/12490/12562/12396/8482/8554/14500-550580 LP446-478503-548579 LP440-472498-540573 LP521/20595 LP463/10523/15585 LP450/10516/20604/22510096101061000v2Optimal template amount for using Kreatech’s ULS labeling kits250 ngAPPLICATIONS2 µgTARGETarrayCGHGenomic DNA20 µgGeneExpressionmicroRNA1 µg1 µg5 µg10 µg10 µgaRNAtotalRNAtotalRNA100 µg DNA/RNATechnical Appendix113
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KREATECH DiagnosticsProduct Guide
Page 4 and 5:
Product Selection GuideI want to us
Page 8 and 9:
HUGO Gene SymbolsThe table below gi
Page 10 and 11:
Oncology probes - Hematology Probes
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Chronic Myeloproliferative Disorder
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ON BCR/ABL t(9;22), DC, S-Fusion, E
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Other Myeloproliferative Diseases:O
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Chronic Lymphocytic Leukemia (CLL)C
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ON hTERC (3q26) / 3q11Amplification
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ON hTERT (5p15) / 5q31The hTERT / 5
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ON MDS 7q- (7q22; 7q35) / SE 7 TCTh
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Acute Myeloid Leukemia (AML)At leas
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ON RARA (17q21) BreakThis break apa
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ON ETV6 (TEL) (12p13) BreakETV6 (TE
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ON MM 1q21 / 8p21Amplifications of
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ON MM 1q21 / SRD (1p36)Frequent los
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ON IGH (14q32) BreakChromosomal rea
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ON BCL6 (3q27) BreakChromosomal tra
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Oncology probes - Solid TumorsIn so
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ON TOP2A (17q21) / Her2 (17q12) / S
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Bladder CancerON p16 (9p21) / 9q21
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NeuroblastomaAccording to the Inter
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ON p53 (17p13) / MPO (17q22) “ISO
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ON SYT (18q11) BreakThe characteris
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ON MDM2 (12q15) / SE 12Fibrosarcoma
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ON PTEN (10q23) / SE 10The gene ‘
Page 62 and 63: ON CCND1 (11q13) / SE 11CCND1 (also
Page 64 and 65: ON BCL6 (3q27) Break (tissue)Chromo
Page 66 and 67: Preimplantation Genetic ScreeningFI
Page 68 and 69: Prenatal probesPrenatal cytogenetic
Page 70 and 71: Microdeletion probesMicrodeletion s
Page 72 and 73: MD DiGeorge “TUPLE” (22q11) / 2
Page 74 and 75: MD NSD1 (5q35) / hTERT (5p15)NSD1 m
Page 76 and 77: MD Angelman UBE3A (15q11) / PML (15
Page 78 and 79: MD Wolf-Hirschhorn WHSC1 (4p16)Wolf
Page 80 and 81: MD STS (Xp22) / KAL (Xp22) / SE X T
Page 82 and 83: Sub-Telomere probesTelomeres are sp
Page 84 and 85: Satellite Enumeration probesThe pri
Page 86 and 87: Whole Chromosome probesThe Poseidon
Page 88 and 89: Arm Specific ProbesArm Specific Pro
Page 90 and 91: Mouse FISH ProbesNew applications f
Page 92 and 93: Chromogenic In Situ HybridizationCh
Page 94 and 95: cell culture mediaKREAvital cytogen
Page 96 and 97: PhytohaemagglutininPhytohaemaggluti
Page 98 and 99: Kits and ReagentsThe ready-to-use p
Page 100 and 101: FISHBright labeling kitsThe FISHBr
Page 102 and 103: Product Selection GuideULSArray CGH
Page 104 and 105: DNA LABELINGULS arrayCGH Labeling K
Page 106 and 107: RNA LABELINGAMPLIFICATION AND aRNA
Page 108 and 109: PROTEIN LABELINGPROTEIN LABELING AN
Page 110 and 111: Antibody LABELINGPlatinumLink Antib
Page 114 and 115: TERMS AND CONDITIONSTerms and Condi
Page 116 and 117: Product IndexOncology probes - Hema
Page 118 and 119: Satellite Enumeration probesSE = Sa
Page 120 and 121: Arm-Specific Probesready-to-useDesc
Page 122 and 123: Mouse FISHDescription Color Tests C
Page 124 and 125: DNA LabelingDescription Reactions C
Page 126 and 127: How To OrderHow to place an orderOr
Page 128: © 2010 KREATECH DiagnosticsPublish
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Oncology Probes

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