Oncology Probes

THE ULS LABELING TECHNOLOGYIntroductionONE LABELING Technology for all Microarray and FISH applicationsThe proprietary ULS (Universal Linkage System) technology provides the basis for KREATECH’s broad rangeof labeling applications. ULS labeling is based on the stable coordinative binding properties of platinum tonucleic acids and proteins. The ULS molecule consists of a platinum complex, a detectable molecule and aleaving group which is displaced upon reaction with the target. This reaction results in a coordinative bond,firmly coupling the ULS to the target. ULS labels DNA and RNA by binding to the N7 position of guanine. Inproteins, ULS binds to sulfur and nitrogen containing side chains of methionine, cysteine and histidine. ULS isavailable coupled to a variety of labels and haptens, including fluorochromes and biotin. The labeling reactionallows a wide range of conditions and produces a highly stable bond.Principle of the ULS technologyTargets labeled with ULSNucleic AcidsGuanineULS labels DNA andRNA by forming acoordinative bondon the N7 positionof guanineProteinsMethionine, Cysteine, HistidineULS labels proteins by forminga coordinative bond on thesulfur atoms of methionine,cysteine and the nitrogen atomof histidineUnique features of the ULS Labeling Technology• Time saving: Entire labeling procedure in only 30 minutesfor DNA and 15 minutes for RNA• Easy to use• Robust and reproducible – no enzymes involved• Superior labeling technology for FFPE samples• One technology for all applications• Compatible with all microarray platformsThe ULS Labeling Technology103