Oncology Probes

ON p53 (17p13) / MPO (17q22) “ISO 17q”Gain of genetic material from chromosome arm 17q (gainof segment 17q21-qter) is the most frequent cytogeneticabnormality of neuroblastoma cells. In multivariate analysis,17q gain was more strongly associated with adverseoutcome than was either stage (Stage 4 vs other combined)or 1p status.ON MDM4 (1q32) / SE1MDM4 (also known as MDMX, murine double minute gene)is a relative of MDM2 that was identified on the basis of itsability to physically interact with p53. MDM4, like MDM2,acts as a key negative regulator of p53 by interfering withits transcriptional activity. MDM4 amplification and/oroverexpression occurs in several diverse tumors. Studiesshowed an increased MDM4 copy number in 65% of humanretinoblastomas compared to other tumors, qualifyingMDM4 as a specific chemotherapeutic target for treatmentof this tumor.The MDM4 (1q32) specific DNA probe is designed as a dualcolorassay to detect amplification at 1q32. The chromosome1 Satellite Enumeration (SE 1) probe at 1qh is included tofacilitate chromosome identification.Cat.# KBI-10011 p53 (17p13) / MPO (17q23) “ISO 17q”Cat.# KBI-10736 MDM4 (1q32) / SE 11qhRH2406MDM4440 KB1q32D1S504See probe description under CML on page 15.1Oncology Probes - Solid TumorsLiterature:Bown et al, 1999, N Engl J Med, 340: 1954-1961.O’Neill, 2001, Genes Chrom Cancer, 30: 87-90.Ordering information Color Tests Cat#ON p53 (17p13) / MPO (17q22) “ISO 17q” red/green 10 KBI-10011MDM4 (1q32) / SE 1 probe hybridized to paraffin embeddedtissue (2R2G).Literature:Riemenschneider et al, 1999, Cancer Res. 59 ; 6091-6096Danovi et al, 2004, Mol.Cell.Bio. 24; 5835-5843Ordering information Color Tests Cat#ON MDM4 (1q32) / SE 1 red/green 10 KBI-1073654