Oncology Probes

ON PML/RARA t(15;17) FusionA structural rearrangement involving chromosomes 15 and 17in acute promyelocytic leukemia (APL) was first recognized in1977. The critical junction is located on the der(15) chromosomeand consists of the 5’ portion of PML fused to virtually all ofthe RARA gene. The PML/RARA fusion protein interacts witha complex of molecules known as nuclear co-repressors andhistone deacetylase. This complex binds to the fusion proteinand blocks the transcription of target genes. Other less commonvariant translocations fuse the RARA gene on 17q21 to thePLZF, NPM, NUMA, and STAT5b genes, respectively.The PML/RARA t(15;17) specific DNA probe is optimized todetect the reciprocal translocation t(15;17) (q24;q21) in a dualcolor,dual-fusion assay.ON MLL (11q23) BreakThe human chromosome band 11q23 is associated with a highnumber of recurrent chromosomal abnormalities includingtranslocations, insertions, and deletions. It is involved inover 20% of acute leukemias. The MLL (Myeloid-LymphoidLeukemia or Mixed-Lineage Leukemia) gene, named forits involvement in myeloid (usually monoblastic) andlymphoblastic leukemia, and less commonly in lymphoma islocated in the 11q23 breakpoint region. Leukemias involvingthe MLL gene usually have a poor prognosis.The MLL (11q23) break probe is optimized to detecttranslocations involving the MLL gene region at 11q23 in adual-color split assay.Cat.# KBI-10302 PML/RARA t(15,17) FusionCat.# KBI-10303 MLL (11q23) BreakRH 54241D17S2189D11S97615PML15q24D15S991420 KB300 KB1717q21RARARH 69878460 KBMLL (88KB)11q23D11S4538220 KB290 KB11Oncology Probes - Hematology ProbesPML/RARA t(15,17) Fusion probe hybridized to a normalmetaphase (2R2G).Literature:Schad et al, 1994, Mayo Clin Proc, 69: 1047-1053.Brockman et al, 2003, Cancer Genet Cytogenet, 145: 144-151.Ordering information Color Tests Cat#ON PML/RARA t(15,17) Fusion red/green 10 KBI-10302ON PML/RARA t(15,17) Fusion red/green 20 KBI-12302MLL (11q23) Break probe hybridized to patient materialshowing a translocation at 11q23 (1RG1R1G).Literature:Kobayashi et al, 1993, Blood, 81: 3027-3022Martinez-Climent et al, 1995, Leukemia, 9: 1299-1304.Ordering information Color Tests Cat#ON MLL (11q23) Break red/green 10 KBI-1030330