Monitoring_Lynx-lynx-carpathicus

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4. Posudzovanie zdravotného stavu a genetika / Health screening and genetics________________________________________________________________________________________parametrov populácie a oportunistickédáta), stopovanie na snehu, vizuálnepozorovania, telemetria. Najvhodnejšievýsledky je možné získať kombinácioutelemetrie a monitoringu s fotopascami.Obr. 1. Proporcia alel v rôznych triedach alelickejfrekvencie rysov na Slovensku na základe 20mikrosatelitov (n=31, period 1).Fig.1. Proportion of alleles in the different allelefrequency classes for lynx in Slovakia based on 20microsatellites (n=31, period 1).________________________________________________________________________________________Monitoring Lynx lynx carpathicus, Rigg & Kubala (2015) 74
4. Posudzovanie zdravotného stavu a genetika / Health screening and genetics________________________________________________________________________________________Assessing genetic health of lynx in SlovakiaCHRISTINE BREITENMOSER-WÜRSTEN & GABRIELA OBEXER-RUFFKORA, Thunstrasse 31, CH 3074 Muri (Bern), Switzerland; ch.breitenmoser@kora.chPopulation monitoring should include not onlyperiodical assessment of numbers, abundance anddistribution, but also of health and genetic status.Monitoring these two parameters was thereforealso included in the project Living with CarpathianSpirits.The lynx population in Slovakia was the sourcefor re-introduction projects in Central and WesternEurope in the 1970s and 1980s. Today several ofthese populations need a genetic remedy asinbreeding has become a serious problem. Ideallythis remedy would happen with lynx from theCarpathian Mts. as they are considered to be adistinct conservation unit. However it is importantto understand the current status before anyfurther animals can be removed.Here we present the results of genetic analysesof 11 samples collected during the project period2013–2014 (period 2) and compare them to 31samples from 1999–2001 (period 1). Our questionswere: 1) How is the genetic variability nowcompared to 12–14 years ago? 2) Does thepopulation show any signs of a bottleneck in thepast? 3) Do we see signs of genetic drift in thecurrent samples that could be caused byfragmentation?Besides answering these questions, the twosets of samples set a baseline for future geneticmonitoring in Slovakia.MethodsIn the frame of a project on lynx genetics calledPopulation and conservation genetics of two reintroducedlynx (Lynx lynx) populations inSwitzerland – a molecular evaluation 30 years aftertranslocation, a panel of 20 microsatellites wasdeveloped to measure genetic variability andpopulation structure across the whole of Europe.We have analysed samples from 8 autochthonousand 5 re-introduced populations to assess theimpact of the artificially created bottlenecks in there-introduced populations (Breitenmoser-Würsten& Obexer-Ruff 2003). The establishedmicrosatellite panel allowed us to run samplescollected during the project Living with CarpathianSpirits with relatively little effort and to comparethem to a set of samples collected 12 years earlier.Extraction: We used the Roche Diagnostics® HighPure PCR Template Preparation Kit for extraction.PCR amplification was performed in a PE 9600thermocycler following the protocol of Menotti-Raymond & O'Brien (1995). The amplifiedfragments were detected using capillary ABIPrism® 3700 Genetic Analyzer. Allele calling wasperformed with GeneMapper 4.0 software(Applied Biosystems, Life Technologies, Carlsbad,California, USA).Statistics: We used the programme GENTIX (Belkhiret al. 1996) to analyse genetic variability(heterozygosity and number of alleles per locus) aswell as differentiation (Fst) between the two timeperiods. We used SPAGeDi (Hardy & Vekemans2002) for the relatedness within the populationusing the r coefficient of Wang (2002). To checkwhether the population shows signs of a formerbottleneck we applied BOTTLENECK (Piry et al.1999), implementing Luikart & Cornuet (1998).Results and discussionGenetic variability: Heterozygosity was similar forboth time periods (0.63 ± 0.11 and 0.61 ± 0.16), thenumber of alleles/locus was higher for period 1________________________________________________________________________________________Monitoring Lynx lynx carpathicus, Rigg & Kubala (2015) 75
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Monitoring stavu karpatského rysav
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Vydané v rámci projektu Spolužit
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1. Úvod / Introduction____________
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2. Princípy monitoringu rysa / Pri
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Page 88 and 89: Literatúra / Literature___________
Page 90 and 91: 1/1Morfologické dáta rysaRys ID:D
Page 92 and 93: 2/3Post-mortem vyšetrenie rysaMakr
Page 94 and 95: 1/6Pitva rysa - patologické vyšet
Page 100 and 101: 1/2Pitva rysa - zoznam vzoriekRys I
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