Chemistry 155 Introduction to Instrumental Analytical Chemistry

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Chem 155 Unit 1 Page 36 of 3136. You need to make a primary stock solution,the concentration of which you know accuratelyand precisely. This solution will usually bemore than twice as concentrated as your mostconcentrated calibration standard. You willdilute this primary stock solution to make thecalibration standards. You need to have enoughto make all of your calibration standards.7. You need to choose the pipets and volumetricflasks that you will use to perform the dilutions.This means that you plan the preparation ofeach standard. This takes some planning andcompromising and many choices – there aremany ways to do this correctly – there is morethan one right answer!8. Decide on and record a labeling system inyour notebook, collect the glassware, and dothe work. I have a labeling system for yourcaffeine, benzoic acid, iron and zinc standards –I need you to use these labels so that we cansort things out in the class.Page 36 of 313
Chem 155 Unit 1 Page 37 of 3131.10.2 Exercise in planning an analysis:Assume that you will be analyzing sucrose in cornsyrup sweetened ketchup packets. The packets arethought (i.e. expected) to contain about 0.8 grams ofcorn syrup that is about 70% sucrose by weight. TheHPLC instrument that you will be using can detectsucrose by refractive index in the 0.1-20 parts perthousand (ppth) range (this is the instrument’sdynamic range for sucrose). You have pure sucrosefor standards, and will be making five calibrationstandard solutions. The instrument requires between250 and 1000 μL of sample.You have the following glassware at your disposal:PipetsVolumetric FlasksVolume RelativePrecisionVolume RelativePrecision20-200 μL 5-1% 1 mL 1%1 mL 1% 5 mL 1%5 mL 1% 10 mL 1%10 mL 1% 25 mL 1%15 mL 1% 50 mL 1%20 mL 1% 100 mL 0.5%25 mL 0.5% 250 mL 0.5%50 mL 0.5% 1000 mL 0.25%Page 37 of 313
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Chemistry 155 Introduction to Instrumental Analytical Chemistry

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