Chemistry 155 Introduction to Instrumental Analytical Chemistry

Chem 155 Unit 1 Page 37 of 3131.10.2 Exercise in planning an analysis:Assume that you will be analyzing sucrose in cornsyrup sweetened ketchup packets. The packets arethought (i.e. expected) to contain about 0.8 grams ofcorn syrup that is about 70% sucrose by weight. TheHPLC instrument that you will be using can detectsucrose by refractive index in the 0.1-20 parts perthousand (ppth) range (this is the instrument’sdynamic range for sucrose). You have pure sucrosefor standards, and will be making five calibrationstandard solutions. The instrument requires between250 and 1000 μL of sample.You have the following glassware at your disposal:PipetsVolumetric FlasksVolume RelativePrecisionVolume RelativePrecision20-200 μL 5-1% 1 mL 1%1 mL 1% 5 mL 1%5 mL 1% 10 mL 1%10 mL 1% 25 mL 1%15 mL 1% 50 mL 1%20 mL 1% 100 mL 0.5%25 mL 0.5% 250 mL 0.5%50 mL 0.5% 1000 mL 0.25%Page 37 of 313