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PRIMARY CARDIOMYOCYTE CULTUREWashing and cell collection:•centrifuge the cell suspension in 2000 rpm for 15 min•remove the supernatant and resuspend the cells in growing medium(DMEM + 10% fetal calf serum (FCS) + gentamicin) – high glucose andglutamine content in the mediumPreplating: fibroblast elimination•plate the cells into 6-well plates for 30 min•collect the supernatant (non-attached cells) into a Falcon tubePlating:•6-well plate we plate 5x10 5 cells/ well•keep them at 37 and 5% CO 2 and change the medium next day (24h)
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