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Basic aseptic conditions• If working on the bench use a Bunsen flame to heatthe air surrounding the Bunsen• Swab all bottle tops & necks with 70% ethanol• Flame all bottle necks & pipette by passing veryquickly through the hottest part <strong>of</strong> the flame• Avoiding placing caps & pipettes down on the bench;practice holding bottle tops with the little finger• Work either left to right or vice versa, so that allmaterial goes to one side, once finished• Clean up spills immediately & always leave the workplace neat & tidy
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