Basics of Cell Culture

More documents

Recommendations

Info

Viability assay – DEAD CELLSTrypane blueexclusionPropidiumiodineBiologicalindicatorDetectionmethodMembraneintegrityColorimetricmicroscopyMembraneintegrityFluorescentFACSTime 2 h 30 minlimitation 24 groups max 10 6 cells/wellrequired + Imre
Viability assay – LIVING CELLSCell-TiterGlo (ATP)Cell TiterBlueresazurinCell Titer96 (MTS)MTTLDHBiologicalindicatorATPcontentReducingabilityReducingabilityReducingabilityMembrane integrityDetectionmethodbioluminescentcolorimet-ricFluorimetricorcolorimetriccolorimetricTime 10 min 1-4 h 1-4 h 4 h 10 minUVSensitivity(96 plate)limitation50 cells 390 cells 800 cells 800 cells 800 cellsLumireaderFluororeaderUVreader
Page 1 and 2: Theoretical course: Basic biochemic
Page 3 and 4: Historical events in the developmen
Page 6 and 7: Continued...• 1965: Harris and Wa
Page 8 and 9: Why is cell culture used for?Areas
Page 10 and 11: Tissue culture• In vitro cultivat
Page 12 and 13: Making a Primary Culture
Page 14 and 15: PRIMARY CARDIOMYOCYTE CULTUREWashin
Page 16 and 17: 2 days old neonatal cardiacmyocyte
Page 18 and 19: Adhesion• Majority Of Cells Adher
Page 20 and 21: Cell Surface Adhesion Molecules•
Page 22: Continous cell lines• Most cell l
Page 25 and 26: Culture media• Choice of media de
Page 27 and 28: Why sub culturing?• Once the avai
Page 29 and 30: Suspension cells• Easier to passa
Page 31 and 32: Freezing cells for storage• Remov
Page 33: Cell toxicity• Cytotoxicity cause
Page 37 and 38: Hemacytometer• Specialized chambe
Page 39 and 40: Looking at the gridunder the phasec
Page 41 and 42: Viability assay - PI fluorescence i
Page 43 and 44: SIMULATEDISCHEMIA(Li et al 2004)CON
Page 45 and 46: Effects of Biological Contamination
Page 47 and 48: Basic equipments used in cell cultu
Page 49 and 50: Water bath• To warm media andPBS
Page 51 and 52: Mechanics of phase microscopyShifti
Page 53 and 54: Basic aseptic conditions• If work
Page 55: Reference:Paras Yadav 1 , Annu Yada

Basics of Cell Culture

Create successful ePaper yourself

Delete template?

Save as template?