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Why sub culturing?• Once the available substrate surface iscovered by cells (a confluent culture) growthslows & ceases.• <strong>Cell</strong>s to be kept in healthy & in growing statehave to be sub-cultured or passaged• It’s the passage <strong>of</strong> cells when they reach to80-90% confluency in flask/dishes/plates• Enzyme such as trypsin, collagenase incombination with EDTA breaks the cellularglue that attached the cells to the surface
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