Basics of Cell Culture

More documents

Recommendations

Info

Contaminant’s of cell cultureCell culture contaminants of two types• Chemical-difficult to detect caused by endotoxins,plasticizers, metal ions or traces of disinfectants thatare invisible• Biological-cause visible effects on the culture theyare mycoplasma, yeast, bacteria or fungus or alsofrom cross-contamination of cells from other cell lines
Effects of Biological Contamination’s• They competes for nutrients with host cells• Secreted acidic or alkaline by-productsceses the growth of the host cells• Degraded arginine & purine inhibits thesynthesis of histone and nucleic acid• They also produces H2O2 which is directlytoxic to cells
Page 1 and 2: Theoretical course: Basic biochemic
Page 3 and 4: Historical events in the developmen
Page 6 and 7: Continued...• 1965: Harris and Wa
Page 8 and 9: Why is cell culture used for?Areas
Page 10 and 11: Tissue culture• In vitro cultivat
Page 12 and 13: Making a Primary Culture
Page 14 and 15: PRIMARY CARDIOMYOCYTE CULTUREWashin
Page 16 and 17: 2 days old neonatal cardiacmyocyte
Page 18 and 19: Adhesion• Majority Of Cells Adher
Page 20 and 21: Cell Surface Adhesion Molecules•
Page 22: Continous cell lines• Most cell l
Page 25 and 26: Culture media• Choice of media de
Page 27 and 28: Why sub culturing?• Once the avai
Page 29 and 30: Suspension cells• Easier to passa
Page 31 and 32: Freezing cells for storage• Remov
Page 33 and 34: Cell toxicity• Cytotoxicity cause
Page 35 and 36: Viability assay - LIVING CELLSCell-
Page 37 and 38: Hemacytometer• Specialized chambe
Page 39 and 40: Looking at the gridunder the phasec
Page 41 and 42: Viability assay - PI fluorescence i
Page 43: SIMULATEDISCHEMIA(Li et al 2004)CON
Page 47 and 48: Basic equipments used in cell cultu
Page 49 and 50: Water bath• To warm media andPBS
Page 51 and 52: Mechanics of phase microscopyShifti
Page 53 and 54: Basic aseptic conditions• If work
Page 55: Reference:Paras Yadav 1 , Annu Yada

Basics of Cell Culture

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?