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Continous cell lines• Most cell lines grow for a limited number <strong>of</strong> generations afterwhich they ceases• <strong>Cell</strong> lines which either occur spontaneously or induced virally orchemically transformed into Continous cell linesCharacteristics <strong>of</strong> continous cell lines-smaller, more rounded, less adherent with a highernucleus /cytoplasm ratio-fast growth and have aneuploid chromosome number-reduced serum and anchorage dependence and grow more insuspension conditions-ability to grow upto higher cell density-different in phenotypes from donar tissue-stop expressing tissue specific genes
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