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Culturing <strong>of</strong> cells• <strong>Cell</strong>s are cultured as anchorage dependent orindependent• <strong>Cell</strong> lines derived from normal tissues are consideredas anchorage-dependent grows only on a suitablesubstrate e.g. tissue cells• Suspension cells are anchorage-independent e.g.blood cells• Transformed cell lines either grows as monolayer oras suspension