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<strong>Cell</strong> toxicity• Cytotoxicity causes inhibition <strong>of</strong> cell growth• Observed effect on the morphological alteration in thecell layer or cell shape• Characteristics <strong>of</strong> abnormal morphology is the giantcells, multinucleated cells, a granular bumpyappearance, vacuoles in the cytoplasm or nucleus• Cytotoxicity is determined by substituting materialssuch as medium, serum, supplements flasks etc. at atime