12.07.2015 Views

Basics of Cell Culture

Basics of Cell Culture

Basics of Cell Culture

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animalNEONATALNewborn- cheap (limitedcell number can beachieved)ADULTAdult –expensive, butmore cellspreparation simple more complicatedsensitivity relatively easy to handle very sensitivelife time couple <strong>of</strong> days, max 2weeks (long termexperiments as well)max 2-3 days (onlyshort term experiments)contamination yes, fibroblasts not observedcoated surface not required exclusively laminincoated surface can beusedmedium DMEM Medium 199 +additional compidentification IH <strong>of</strong> cardiomyocytes microscopic morphologyadherent yes yes

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