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SIMULATEDISCHEMIA(Li et al 2004)CONTROL SOLUTIONMolecularweightmM For 1 L (g) For 100 ml(mg)NaCl 58,44 125 7,305 730KCl 74,56 5,4 0,402 40,2NaH 2 PO4.1 H 2 O 137,99 1,2 0,1655 16,6MgCl 2 (saturated95,23 0,5 126 ul 12,6 ulsolution)HEPES 238,3 20 4,766 476,6glucose 198,18 15 2,972 297,2taurine 125,1 5 0,6255 62,6CaCl 2 .2 H 2 O 174,02 1 0,174 17,4(Creatine P.Na 2 .6 H 2 O) 363,2 2,5 0,908 90,8creatine 131,14 2,5 0,328 32,79BSA 0,1% 100pH: set to 7,4 (pH 6 was after mixing components)MgCl 2 (saturated solution): 1 ml contains 0,3774 gHYPOXIC SOLUTIONMolecularweightmM For 1 L (g) For 100 ml(mg)NaCl 58,44 119 6,954 695,4KCl 74,56 5,4 0,402 40,2NaH 2 PO4.1 H 2 O 137,99 1,2 0,1655 16,6MgCl 2 (saturated95,23 0,5 126 ul 12,6 ulsolution)HEPES 238,3 5 1,1915 119,2MgSO 4 .7 H 2 O 246,48 1,3 0,320 32CaCl 2 .2 H 2 O 174,02 0,9 0,1566 15,7Na-lactate 112,1 20 2,242 224BSA 0,1% 100pH: set to 6,4 (pH 6,4 was after mixing components)MgCl 2 (saturated solution): 1 ml contains 0,3774 g
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