12.07.2015 Views

Basics of Cell Culture

Basics of Cell Culture

Basics of Cell Culture

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Viability assay – LIVING CELLS<strong>Cell</strong>-TiterGlo (ATP)<strong>Cell</strong> TiterBlueresazurin<strong>Cell</strong> Titer96 (MTS)MTTLDHBiologicalindicatorATPcontentReducingabilityReducingabilityReducingabilityMembrane integrityDetectionmethodbioluminescentcolorimet-ricFluorimetricorcolorimetriccolorimetricTime 10 min 1-4 h 1-4 h 4 h 10 minUVSensitivity(96 plate)limitation50 cells 390 cells 800 cells 800 cells 800 cellsLumireaderFluororeaderUVreader

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