UROP Proceedings 2010-11

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After a month of screening, some putative mutants with extended body length were isolated.On the basis of a change of body length, the next phase is to map the gene by genetic linkageand molecular mapping procedures. We hope to confirm the exact location of the mutation onspecific chromosome and eventually elucidate its molecular identity to understand how theTGF beta pathway is being modulated.Mechanosensitive Ion Channels in Human Embryonic and InducedPluripotent Stem CellsAdvisor : HUANG Pingbo / LIFSStudent : LIN Qiaojin / BIOL(UROP1100, Spring 2011)This project mainly focuses on investigating mechanosensitivity of ion channels by applyingpatch-clamp technique. Mechanosensitivity has been found in mechanosensitive ion channels,which can be stimulated by touch, hearing and balance. Scientific research suggests thesechannels play important roles in osmotic homeostasis and cardiovascular regulation.During the process, I gained lab skills in handling mammalian cells, and I had an opportunityto have a taste in doing scientific research. In the next phase, I will continue with the patchclamp experiment on T84 cells, with an aim to record steadier channel activity.Mechanosensitive Ion Channels in Human Embryonic and InducedPluripotent Stem CellsAdvisor : HUANG Pingbo / LIFSStudent : LIN Qiaojin / BIOL(UROP1200, Summer 2011)This is the second semester for me to participate in this project, and I have learnt much morein research techniques. I have been mainly working with T84 cell line, which is an excellentmodel system for studying electrolyte transport processes and their regulation by peptidehormones and neurotransmitters. Another part of my project is the cloning of shRNA, whichis a small fragment of RNA that makes a tight hairpin shape, and it shows the ability tosilence gene expression applying RNA interference mechanism. I am planning to continue inthis project in the Fall semester, and I look forward to getting hands-on practice on Ussingchamber, and to further develop my molecular cloning technique.3
The Effect of Adhesion on Cell Polarization and Cloning of shRNAAdvisor : HUANG Pingbo / LIFSStudent : MOK Ka Yi / MBMS(UROP1000, Summer 2011)Several studies have been done on investigating the effect of adhesion on cell polarization.Inverted culture on dissociated mouse hippocampal neurons was proven to have improvedneuronal culture condition. Hippocampal neurons undergo polarization and forms dendriticspines in inverted culture. Nowadays, shRNA was commonly used to knockdown the targetgene, and it is an effective and stable system to investigate the loss of function of the targetgene.This project consists of two parts. In Part A, the mechanosensitive response of 293T cell lineand Calu-3 cell line other than neuron cell are investigated. In Part B, cloning of shRNA isdone to knockdown the tumor suppressor gene.Die-off Rate of Sewage-associated Bacteria in the Marine Environmentof Hong KongAdvisor : LAU Stanley C K / LIFSStudent : TAI Man Yi / BIOL(UROP1100, Spring 2011)The quality of recreational seawater depends on the number of pathogenic bacteria present.The chances that beach goers contract gastrointestianl diseases are directly proportional to theamount pathpgenic bacteria present in seawater. Sewage effluents are an imporat source ofpathogenic bacteria. This project aims to find out the relationship between spatial andseasonal factors and the die-off rate of sewage-associated bacteria, and to suggest ways tomodify the monitoring method of seawater in Hong Kong.Decay Rate of Sewage-associated Bacteria in the Marine Environment ofHong KongAdvisor : LAU Stanley C K / LIFSStudent : WONG Klaine / BIOL(UROP1100, Spring 2011)Enterococci is used as an indicator of seawater quality in many countries such as the US andSingapore. On the contrary, Hong Kong relies on Escherichia coli. This project investigatedthe die-off rates of enterococci in the marine environment of Hong Kong. The aim was toevaluate if enterococci would be a suitable pollution indicator for the marine environment ofHong Kong.4
Page 4 and 5: Message from the PresidentUROP is a
Page 6 and 7: School ofScience
Page 10: Water Quality Survey for Hong Kong'
Page 14 and 15: Study of Blood Cell Development usi
Page 16 and 17: Department of MathematicsSpace Orbi
Page 18 and 19: Report on Lunar Crater ProjectAdvis
Page 20 and 21: Efficient Numerical Methods for Dyn
Page 22 and 23: Department of PhysicsNear-Field Sca
Page 24 and 25: Kinetic Monte Carlo Simulation of S
Page 26 and 27: Random Walk on Complex Network and
Page 28 and 29: Manufacture of Photoic Crystals and
Page 30 and 31: School ofEngineering
Page 32 and 33: Techno-economic Analysis of Hydroge
Page 34 and 35: Heavy Metal Adsorption from Seawate
Page 36 and 37: Reformation of Antimicrobial Coatin
Page 38 and 39: Innovative Iron-based Process for O
Page 40 and 41: Department of Computer Science and
Page 42 and 43: An Investigation to Simplex Minimiz
Page 44 and 45: Distributive Name ServiceAdvisor :
Page 46 and 47: “Where To”: A Collaborative And
Page 48 and 49: Light Carving: Rich Object Scanning
Page 50 and 51: Sparse Matrix CompletionAdvisor : Y
Page 52 and 53: Optical Property Investigation of N
Page 54 and 55: Opto-Mechanical Oscillator with Dir
Page 56 and 57: Capture the FlagAdvisor : SHI Ling
Page 58 and 59:
Building a Servo Robot SystemAdviso
Page 60 and 61:
Department of Industrial Engineerin
Page 62:
Digital Image Correlation Method fo
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Department of AccountingSchool of B
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Department of EconomicsThe Microeco
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Department of Information Systems,
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Financial Risk ManagementAdvisor :
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Consumer Shopping Behavior in Inter
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Consumer NostalgiaAdvisor : HONG Ji
Page 77 and 78:
Lay Theory of ObesityAdvisor : MUKH
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Multiculturalism, Social Judgments
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School of Humanities and Social Sci
Page 83 and 84:
China/Africa Links ProjectAdvisor :
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Housing Policy, Home Ownership Sche
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Psychology of HappinessAdvisor : TA
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Facial Expressions of EmotionsAdvis
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UROP Proceedings 2010-11

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