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Symbiotic Fungi: Principles and Practice (Soil Biology)

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14 Micro-PIXE Analysis for Localization <strong>and</strong> Quantification of Elements 237<br />

b<br />

SM<br />

P S<br />

Cl K Ca<br />

Fe<br />

Cu Zn<br />

0 High counts<br />

Fig. 14.5 (a) Element maps of Viola lutea ssp. westfalica root samples collected at the nonpolluted<br />

site. Scan size 250 250 mm 2 . SM – photograph of the sample taken under an optical stereomicroscope.<br />

(b) Element maps of Viola lutea ssp. westfalica root samples collected at the metalpolluted<br />

site. Scan size 250 250 mm 2 . SM – photograph of the sample taken under an optical<br />

stereo-microscope. An arbuscular mycorrhizal structure is indicated by an arrow<br />

characteristic X-ray counts (PIXE spectra), proton dose, sample matrix density<br />

(cellulose), <strong>and</strong> thickness of the sample as determined by STIM.<br />

The determination of concentrations in selected plant tissues allows one to<br />

compare the element distributions in differently treated plants, <strong>and</strong>/or to assess<br />

shifts in element depositions caused by mycorrhization, in a similar way to that<br />

described by Scheloske et al. (2004).

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