INSTITUTUL NAÅ¢IONAL DE CERCETARE-DEZVOLTARE - ICECHIM

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1 Bioresources and biomaterials - PSCREEN PRINTED IMPEDIMETRIC IMMUNOSENSOR FOR SENSITIVEDETERMINATION OF AFLATOXIN B1Emilia OCNARU 1 , Mihaela BADEA-DONI 1 , Melania-Liliana ARSENE 1 ,Fabiana ARDUINI 2 , Andrei-Florin DANET 31 National Research & Development Institute for Chemistry & Petrochemistry - ICECHIM,Spl. Independentei 202, Bucharest, ROMANIA2 Universita degli Studi “Tor Vergata”, Dipartimento di Scienze e Techologie Chimiche, Viadella Ricerca Scientifica – 00133 Roma, ITALIA3 University of Bucharest, Faculty of Chemistry, Sos. Panduri 90-92, Bucharest, ROMANIAThe aim of this work was the development of a disposable impedimetric immunosensor forthe detection of Aflatoxin B1. The proposed immunosensor is based on indirect competitiveELISA procedure performed on screen-printed gold electrodes (Au-SPEs) in the presence ofanti-AFB1 antibodies. The immunosensor strips were assembled by covalent immobilizing ofthe biological component (the AFB1 conjugated to bovine serum albumin) to a self-assembledthiol monolayer on gold. Then, the immunosensors were incubated with samples (orstandards) and the antibody anti-AFB1 in order to have a competition between the free AFB1and the AFB1 bound to the Au-SPE surface for the antibody Anti-AFB1. The EIS signalsobtained were dependent on the free AFB1 concentration present in sample.The method proposed here avoids the use of an immunoaffinity column, which is anexpensive and time-consuming step in sample preparation for AFB1 determination by thestandard HPLC method. Preliminary data indicate that the developed electrochemicalimmunosensor is acceptable for analyses of AFB1 at the level required by the official EUregulations.[1]. B.Prieto-Simon, M. Campas, J.L. Marty, T. Noguer, Biosensors and Bioelectronics 23(2008). 995-1002[2]. J.H. Owino, A. Ignaszak, A. Al-Ahmed, P.G. Baker. H. Alemu, J. C. Ngila, E. I. Iwuoha,Anal. Bioanal. Chem (2007), 388:1069-1074
1 Bioresources and biomaterials - PIMMOBILIZATION OF ALKALINE PHOSPHATASE ON BIOPOLYMERICSUPPORTLuminita Tcacenco*, Iordachel Catalin*, Elena Berteanu*, Ioana Demetrescu **,Daniela Ionita**, Mihaela Ionica Enache*, Adina LIDIA Zuav*,*National Institute of Research and Development for Biological Sciences, Bucharest,Romania phone/Fax+4021.220.79.09; lili_berteanu@yahoo.com**Politehnica Bucharest University, Faculty of Applied Chemistry and Material ScienceDepartment of General Chemistry, Polizu Street, Bucharest, 011061Abstracts: The enzyme immobilization in general, and immobilisation of the alkalinephosphatase on a biopolymeric support especially, constitutes, at present, a much studiedtechnique which has a great importance from the point of view of its practical and scientificapplications.The objective of this study was to obtain new nanostructured composite materials,composed primarily of products based on alkaline phosphatase-ALP (EC3.1.3.1.) and naturalpolymers, collagen and chitosan.Thus we have developed a laboratory scale experimental method for alkalinephosphatase immobilization by physical adsorption on biopolymer support method.Varioustypes of scaffolds were prepared by varying compositions of alkaline phosphatase, collagenand chitosan. The scaffolds were fully characterized by Fourier transform infrared (FT-IR)spectroscopy.The results obtained are promissing regarding the use of these supports to ALPimmobilization, the enzyme having a large practical use, opening a new perspective for its usein different skin tissue engineering.(1,2)References:1. JUN F. LIANG,* YONG T. LI, VICTOR C. YANG, Biomedical Application of Immobilized Enzymes,College of Pharmacy, The University of Michigan, 15 March 2000.2.CHALONGLARP TANGSADTHAKUN1, SORADA KANOKPANONT1, NEERACHASANCHAVANAKIT2, TANOM BANAPRASERT3, SIRIPORN DAMRONGSAKKUL1, Properties ofCollagen/Chitosan Scaffolds for Skin Tissue Engineering, Journal of Metals, Materials and Minerals. Vol. 16No.1 pp.37-44, 2006.
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INSTITUTUL NAÅ¢IONAL DE CERCETARE-DEZVOLTARE - ICECHIM

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