INSTITUTUL NAÅ¢IONAL DE CERCETARE-DEZVOLTARE - ICECHIM

4. Protection and environmental engineering - PPHYSIOLOGICAL AND ENVIRONMENTAL FACTORS AFFECTING THEGROWTH AND SPORULATION OF TRICODERMA SP.Iuliana RAUT 1 , Mariana CALIN 1 , Luiza JECU 1 , Tatiana Eugenia SESAN 2[1] INCDCP-ICECHIM, Spl. Independentei 202, Bucharest[2] University of Bucharest, Facultaty of Biology, Spl. Independentei 91-95, BucharestTrichoderma is considered to be one of the most versatile and effective biocontrol agentsstudied 1 . The species contains an arsenal of mechanisms and antimicrobial metabolitescapable to fight against different pathogens. Optimization of Trichoderma culture conditionsis the preliminary step towards the selection of most efficient antagonistic strains 2, 3, 4 .The objective of this study was to investigate the influence of physiological andenvironmental factors upon the growth and sporulation of antagonistic Trichoderma sp.T36.Some important parameters to be considered are: culture medium composition; influence ofcarbon and nitrogen source; the effects of temperature and pH. The influence of tested factorswas evaluated with the colony diameter of microbial culture grown on solid medium in Petriplates. The growth was favoured by PDA, MEA OM, M1 nutrients media, with best results onM1 medium. This medium was selected for further experiments concerning the study ofvarious carbon and nitrogen source. A wide range of carbohydrates as carbon source has beentested, among them the best results were obtained in media with ribose, D mannose, fructose,dextrose and arabinose, around 6.75 cm diameter of colony zone. Also citric acid was foundto be an good and rapid available carbon source. It was investigated the effect of inorganicand organic nitrogen source. The lowest diameter colony was found for KNO 3 , KNO 2 andNH 4 Cl while the highest were for NaNO 3 and NaNO 3. Trichoderma sp. T36 presented a goodgrowth and sporulation on media supplemented with valine, alanine, L lisine, isoleucine and Lserine. As regarding the environmental factors, Trichoderma growth was facilitated incultures performed at 25-27°C temperature and pH from 4.0 to 5.5.Bibliografie1. Monte ,E., 2001, Understanding Trichoderma: between biotechnology and microbial ecology, Int.Microbiol., 4:1-4.2. Jayaswal R. K., Rajesh S. and Lee Y. S., 2003. Influence of physiological and environmental factor ongrowth and sporulation of an antagonistic strain of Trichoderma viride RSR 7, Mycobiol. 31(1): 36-41.3. Sesan T. E. and Oancea F., 2010. Trichoderma viride pers.- Experimental model for biological andbiotechnological investigations of mycromyceta with importance in obtaining plant protection bioproduct. J.Plant Develop., 17: 49-62.4. Torres N. V., Vasques F.A. & Voit E. O., 2004. Introduction to the theory of metabolic modeling andoptimization of biochemical systems, in Arora. D. K., Ed.(ed.), Handbook of Fungal Biotechnology (2endedition), CRC Press/Marcel Dekker, New York, USA: 353-366.